Abstract:
OBJECTIVE:To investigate the effects of red orpiment on cell morphology, expression of promyelocytic leukemia (PML) mRNA and its protein localization in NB4 and HL-60 cell lines. METHODS:Cell morphology was assayed by Wright's staining and fluorescence staining, while PML mRNA expression was determined by RT-PCR. PML protein localization by evaluated by immunofluorescence staining. RESULTS:The typical apoptosis was found in NB4 and HL-60 cells after treatment with red orpiment. The fusion protein was no longer observed in NB4 cells, PML protein was relocated, and then degraded. In HL-60 cells, PML protein underwent a similar progress. The expression of promyelocytic leukemia (PML) mRNA was not changed in the treated cells. CONCLUSION:Red orpiment inhibits the proliferation of leukemia cells by inducing them to undergo apoptosis.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Zhong L,Chen F,Han J,Shao N,Ouyang Rkeywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
148-50issue
1eissn
0366-6999issn
2542-5641journal_volume
116pub_type
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