Abstract：
:There is considerable current interest in the neuroprotective effects of flavonoids. This study focuses on the potential for dietary flavonoids, and their known physiologically relevant metabolites, to enter the brain endothelium and cross the blood-brain barrier (BBB) using well-established in vitro models (brain endothelial cell lines and ECV304 monolayers co-cultured with C6 glioma cells). We report that the citrus flavonoids, hesperetin, naringenin and their relevant in vivo metabolites, as well as the dietary anthocyanins and in vivo forms, cyanidin-3-rutinoside and pelargonidin-3-glucoside, are taken up by two brain endothelial cell lines from mouse (b.END5) and rat (RBE4). In both cell types, uptake of hesperetin and naringenin was greatest, increasing significantly with time and as a function of concentration. In support of these observations we report for the first time high apparent permeability (Papp) of the citrus flavonoids, hesperetin and naringenin, across the in vitro BBB model (apical to basolateral) relative to their more polar glucuronidated conjugates, as well as those of epicatechin and its in vivo metabolites, the dietary anthocyanins and to specific phenolic acids derived from colonic biotransformation of flavonoids. The results demonstrate that flavonoids and some metabolites are able to traverse the BBB, and that the potential for permeation is consistent with compound lipophilicity.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Youdim KA,Dobbie MS,Kuhnle G,Proteggente AR,Abbott NJ,Rice-Evans Cdoi
10.1046/j.1471-4159.2003.01652.xkeywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
180-92issue
1eissn
0022-3042issn
1471-4159pii
1652journal_volume
85pub_type
杂志文章abstract：:The binding of [3H]5-hydroxytryptamine (5-HT, serotonin) to cerebellar membranes was examined after preincubation of [3H]5-HT in the presence or absence of ascorbate. The tissue preparation was identical in all experiments and consisted of rat cerebellar homogenates in Tris-HCl buffer with 0.1% ascorbate. Cerebellar m...
journal_title：Journal of neurochemistry
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journal_title：Journal of neurochemistry
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journal_title：Journal of neurochemistry
pub_type： 杂志文章,评审
doi：10.1111/jnc.13623
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.2006.03886.x
更新日期：2006-07-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1985-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：1997-06-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1046/j.1471-4159.2001.00255.x
更新日期：2001-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.1990.tb04917.x
更新日期：1990-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1046/j.1471-4159.1996.66041717.x
更新日期：1996-04-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/jnc.13612
更新日期：2016-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.2005.03512.x
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pub_type： 杂志文章
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1993.tb13410.x
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pub_type： 杂志文章
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更新日期：2004-08-01 00:00:00
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pub_type： 杂志文章
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doi：10.1046/j.1471-4159.1999.0731181.x
更新日期：1999-09-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1986.tb13015.x
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1046/j.1471-4159.1995.65031213.x
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abstract：:Spinocerebellar ataxia type 3 (SCA3) is an autosomal dominantly inherited neurodegenerative disorder for which no curative therapy is available. The cause of this disease is the expansion of a CAG repeat in the so-called ATXN3 gene leading to an expanded polyglutamine stretch in the ataxin-3 protein. Although the func...
journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/jnc.13606
更新日期：2016-07-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1989.tb07249.x
更新日期：1989-06-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1988.tb03043.x
更新日期：1988-05-01 00:00:00
abstract：:The immediate acyl chain donor for fatty acid esterification of proteolipid protein (PLP) was identified in an in vitro system. Rat brain total membranes, after removal of crude nuclear and mitochondrial fractions, were incubated with radioactive acyl donors, extracted with chloroform/methanol, and analyzed by sodium ...
journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1986.tb13013.x
更新日期：1986-02-01 00:00:00
abstract：:In this review, we discuss the poorly explored role of calcium/calmodulin-dependent protein kinase II (CaMKII) in memory maintenance, and its influence on memory destabilization. After a brief review on CaMKII and memory destabilization, we present critical pieces of evidence suggesting that CaMKII activity increases ...
journal_title：Journal of neurochemistry
pub_type： 杂志文章,评审
doi：10.1111/jnc.14454
更新日期：2018-10-01 00:00:00
