Abstract:
PURPOSE:TNFalpha is a strong modulator expression of trabecular meshwork (TM) matrix metalloproteinase (MMP) and tissue inhibitor (TIMP). Laser trabeculoplasty appears to rely on this process to restore normal aqueous humor outflow facility. Thus, studies were conducted to determine whether the extracellular signal-regulated kinase (Erk)-mitogen-activated protein (MAP) kinase signal-transduction pathway is involved. METHODS:Porcine TM cells were treated with TNFalpha, and changes in MMPs and TIMPs were evaluated by zymography and Western immunoblot assay. Phosphospecific antibodies to proteins from the Erk pathway were used to evaluate responses to treatment with TNFalpha. Inhibitors of Mek, the kinase that activates Erk, and of protein kinase C (PKC) isoforms were used to define pathway involvement. RESULTS:Treatment with TNFalpha increased MMP-1, -3, and -9 and TIMP-1, whereas expression of MMP-2 was not affected and expression of TIMP-2 was decreased. Erk and Mek were rapidly phosphorylated after treatment with TNFalpha, and c-Raf-1 showed a significant bandshift. A specific inhibitor of Mek blocked the TNFalpha induction of the MMPs and TIMPs and the phosphorylation of Erk. An inhibitor of the PKC- micro isoform, which also blocks the effects of MMP-TIMP of TNFalpha, did not affect phosphorylation of Erk. CONCLUSIONS:The components of this MAP kinase pathway in the TM are dramatically affected by TNFalpha and inhibition of Erk's phosphorylation blocks the changes in MMP and TIMP expression. PKC micro, which is also required in this transduction process, does not appear to be upstream from Erk in the signaling cascade. Manipulation of this and related TM signal-transduction pathways may provide targets for developing improved glaucoma treatments.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Alexander JP,Acott TSdoi
10.1167/iovs.01-1201keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
164-9issue
1eissn
0146-0404issn
1552-5783journal_volume
44pub_type
杂志文章abstract:Purpose:Although normal function of the lacrimal gland is essential for vision (and thus for human well-being), the lacrimal gland remains rather poorly understood at a molecular level. The purpose of this study was to identify new genes and signaling cascades involved in lacrimal gland development. Methods:To identif...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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journal_title:Investigative ophthalmology & visual science
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更新日期:2015-08-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1999-10-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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pub_type: 杂志文章
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journal_title:Investigative ophthalmology & visual science
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更新日期:2012-01-10 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.18-25920
更新日期:2019-03-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1980-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1167/iovs.11-9034
更新日期:2012-10-17 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:2001-08-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
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更新日期:2018-08-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
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更新日期:2017-06-01 00:00:00
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更新日期:2012-05-14 00:00:00