Abstract：
:An enzyme-linked immunosorbent assay (ELISA) has been developed for a conjugated bile acid, 7alpha,12alpha,24-trihydroxy-5alpha-cholan-3-one 24-sulfate (commonly referred to as 3-keto petromyzonol sulfate [3kPZS]), a pheromone released by reproductively mature male sea lampreys to attract sexually mature females. A polyclonal antiserum against the pheromone was raised by injecting 3-keto petromyzonol 24-hemisuccinate (3kPZ-HS) conjugated to bovine serum albumin into rabbits. The enzyme label was prepared by conjugating 3kPZ-HS to acetylcholinesterase. The standard curve had a working range of 20 pg-10 ng/well. Intra- and inter-assay variations were less than 5 and 12%, respectively. The antiserum had 100% cross-reaction with 3-keto petromyzonol and 3-keto allocholic acid but less than 0.2% cross-reaction with petromyzonol, allocholic acid, cholic acid, and taurolithocholic acid sulfate. The assay was applied to water which had been conditioned for 4h by either larvae, parasitic juveniles, ovulating females, pre-spermiating males, or spermiating males. Immunoactive material (average 200 ng/ml, which is equivalent to 500 microg animal/h) was only found in water from the reproductively mature males and diluted parallel with the standard curve. Assay of water samples collected from male lampreys in bisected aquaria also established that 99.6% of the immunoactive material emanated from the front end of the fish. This assay has applications in both physiological and ecological aspects of sea lamprey reproduction.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Yun SS,Scott AP,Siefkes MJ,Li Wdoi
10.1016/s0016-6480(02)00517-8keywords:
subject
Has Abstractpub_date
2002-12-01 00:00:00pages
163-70issue
3eissn
0016-6480issn
1095-6840pii
S0016648002005178journal_volume
129pub_type
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journal_title：General and comparative endocrinology
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doi：10.1006/gcen.1994.1029
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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doi：10.1006/gcen.2001.7772
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2000.7541
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journal_title：General and comparative endocrinology
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doi：10.1016/0016-6480(91)90156-z
更新日期：1991-09-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2019.03.001
更新日期：2019-05-15 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90048-b
更新日期：1991-11-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2005.08.002
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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abstract：:In fish, melatonin is reported to be produced mainly in the pineal organ, but there is also evidence for the presence of melatonin in a number of extrapineal sites where it could act as an intracellular mediator or paracrine signal. The present study use the reverse transcription-polymerase chain reaction (RT-PCR) to ...
journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2000.7469
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1995.1087
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2007-06-01 00:00:00
abstract：:Bicuspid teeth are a morphological characteristic in adult metamorphosed urodeles. However, during the mating season the males of many species of plethodontid salamanders (Urodela, Plethodontidae) from northern America bear large monocuspid teeth on their jaws. Since androgen levels are elevated during the mating seas...
journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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doi：10.1006/gcen.1997.7051
更新日期：1998-05-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2003-03-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2013-11-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2003.08.012
更新日期：2004-01-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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abstract：:Species differences in the effect of male gonadal androgens on male growth are considered a possible mechanism allowing shifts in magnitude and even direction of sexual size dimorphism (SSD) in squamate reptiles. According to the bipotential growth regulation hypothesis, the androgen testosterone (T) enhances male gro...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2013-07-01 00:00:00
