Abstract:
:Angiogenesis in pancreatic ductal adenocarcinomas depends on the presence of angiogenic factors such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) and is thought to be stimulated by hypoxia. We tested the angiogenic potential of 9 cell lines of pancreatic ductal carcinoma origin by screening mRNA and protein expression of VEGF and bFGF and the release of VEGF into culture medium under normoxic and hypoxic (5% or 0.2% O2) conditions. Angiogenic activity was determined using 2- and 3-D endothelial cell assays. Furthermore, VEGF expression and tumor vascularization were studied in human pancreatic carcinoma tissues from orthotopic xenografts and resection specimens. All cell lines expressed (mRNA, protein) and secreted VEGF, whereas bFGF was only found in 3 cell lines and was secreted into the medium in low concentrations. In addition to the dominant isoforms VEGF121,VEGF165 and VEGF189, 2 isoforms described recently, VEGF145 and VEGF183, were detected. Severe hypoxia (0.2% O2), but not moderate hypoxia (5% O2) raised VEGF mRNA expression and protein secretion in 7/9 and 5/9 cell lines, respectively. Conditioned media from 7/9, 6/9, 8/9 and 7/9 cell lines stimulated endothelial cell proliferation under normoxic (24 and 48 hr) or hypoxic (24 hr, 0.2% and 48 hr 5% O2) conditions, respectively. Conditioned media from 4/9 cell lines also induced capillary-like sprouting under normoxic conditions and from 6/9 under hypoxic (0.2% O2) conditions. In xenografted carcinoma tissues microvessel density was found not to be increased around areas of ischemic necrosis. In resected ductal carcinomas showing tumor necrosis VEGF expression and microvessel density were only increased in 3/12 and 2/13 cases, respectively. In conclusion, in vitro most pancreatic ductal carcinomas show a distinct VEGF related angiogenic potential, as demonstrated by 2- and 3-D endothelial cell proliferation, which may be promoted by severe hypoxia. Surprisingly, perinecrotic tumor areas, which are supposed to be hypoxic, only rarely showed the expected increase in microvessel density and VEGF expression.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Sipos B,Weber D,Ungefroren H,Kalthoff H,Zühlsdorff A,Luther C,Török V,Klöppel Gdoi
10.1002/ijc.10753keywords:
subject
Has Abstractpub_date
2002-12-20 00:00:00pages
592-600issue
6eissn
0020-7136issn
1097-0215journal_volume
102pub_type
杂志文章abstract::We have addressed the role of bacterial co-infection in viral oncogenesis using as model Epstein-Barr virus (EBV), a human herpesvirus that causes lymphoid malignancies and epithelial cancers. Infection of EBV carrying epithelial cells with the common oral pathogenic Gram-negative bacterium Aggregatibacter actinomycet...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31652
更新日期:2019-01-01 00:00:00
abstract::Serum tyrosinase activity has been measured by adapting the [3]tyrosine assay for tyrosinase and significant elevations of serum tyrosinase activity were found in patients with malignant melanoma. In contrast to findings in a study which utilized [14C]tyrosine, augmented levels of tyrosinase activity were not observed...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910200507
更新日期:1977-11-15 00:00:00
abstract::We report on pigmentary characteristics, sun sensitivity and some other possible risk factors for non-Hodgkin lymphoma (NHL) in people 20-74 years of age. A statewide case-control study was conducted in New South Wales, Australia, with population-based sampling of cases (n = 704) and controls (n = 694). Risk of NHL wa...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20128
更新日期:2004-06-20 00:00:00
abstract::Since no large descriptive studies of incident cancers in HIV-infected patients are available in France, the nationwide cross-sectional ONCOVIH study aimed to prospectively report new malignancies diagnosed in HIV-infected patients in cancer centers and HIV/AIDS centers. We estimated the number of cancers in France fo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25903
更新日期:2011-07-15 00:00:00
abstract::There is a familial aggregation of prostate cancer, and 5 to 10% of all prostate cancers are estimated to be inherited in an autosomal-dominant mode. A population-based cohort study was performed in order to study familial prostate cancer and associated malignancies. A nation-wide register cohort study was conducted u...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19981029)78:3<293::AID-IJC
更新日期:1998-10-29 00:00:00
abstract::To identify characteristics of gastric cancer associated with hereditary non-polyposis colorectal cancer (HNPCC), we gathered clinical data and tumor samples relating to patients recorded in the Finnish HNPCC registry. Our series included 51 families with a characterized mutation and/or that met the Amsterdam criteria...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19971021)74:5<551::aid-ijc
更新日期:1997-10-21 00:00:00
abstract::Protocadherin 10 (PCDH10), a novel tumor suppressor gene in human cancers, is located in a common deleted region at chromosome 4q28 in colorectal cancer (CRC). This study aimed to ascertain the genetic loss of PCDH10 and its clinical relevance in CRC and to explore the tumor suppressor function of PCDH10. The genetic ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28899
更新日期:2014-12-01 00:00:00
abstract::Testicular germ cell tumors (TGCTs) are a clinically and pathologically heterogeneous disease, and little is known of its genetic basis. Only low susceptibility risk loci have been identified for both sporadic and familial cases. Therefore, we tried to identify new susceptibility genes responsible for familial testicu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31604
更新日期:2018-10-15 00:00:00
abstract::A recent clinical trial found a protective role of niacinamide, a derivative of niacin, against skin cancer recurrence. However, there is no epidemiologic study to assess the association between niacin intake and risk of skin cancer [basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and melanoma]. We prospecti...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30630
更新日期:2017-05-01 00:00:00
abstract::To better inform the next clinical trials of vascular disrupting agent combretastatin-A4-phosphate (CA4P) in patients with hepatic malignancies, this preclinical study aimed at evaluating CA4P therapeutic efficacy in rats with primary hepatocellular carcinomas (HCCs) of a full spectrum of differentiation and vasculari...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31567
更新日期:2018-10-01 00:00:00
abstract::The International Classification of Childhood Cancer (ICCC) updates the widely used Birch and Marsden classification scheme. ICCC is based on the second edition of the International Classification of Diseases for Oncology (ICD-O-2). The purpose of the new classification is to accommodate important changes in recogniti...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0215(19961211)68:6<759::AID-IJC
更新日期:1996-12-11 00:00:00
abstract::Acrylamide, a probable human carcinogen, is formed during the cooking of many commonly consumed foods. Data are scant on whether dietary acrylamide represents an important cancer risk in humans. We studied the association between acrylamide and prostate cancer risk using 2 measures of acrylamide exposure: intake from ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24175
更新日期:2009-05-15 00:00:00
abstract::The granulin-epithelin precursor (GEP/PCDGF), a 68-88 kDa secreted glycoprotein, has been shown to be an important growth and survival factor for ovarian cancer cells. Furthermore, GEP expression is a predictor of patient survival in metastatic ovarian cancer cells. Up to this point, however, the molecular mechanisms ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22559
更新日期:2007-06-01 00:00:00
abstract::Macrophages were isolated from 22 human ascitic ovarian epithelial tumors and their growth-inhibitory capacity was tested using as targets the following in vitro tumor cell lines: murine TLX9 lymphoma and FS6 sarcoma; human myeloid K562 leukemia and human E cell line derived from an ovarian carcinoma. Macrophage prepa...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910230204
更新日期:1979-02-01 00:00:00
abstract::Dose-dense adjuvant chemotherapy is standard of care in high-risk early breast cancer patients. However, its role in HER2-positive patients is still uncertain. In this exploratory analysis of the GIM2 trial, we investigated the efficacy of dose-dense chemotherapy in HER2-positive breast cancer patients with or without...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32789
更新日期:2020-07-01 00:00:00
abstract::In a retrospective analysis of 203 patients with small cell lung cancer (SCLC), we examined the prognostic value of c-kit expression on survival. Expression of c-kit was examined immunohistochemically in formalin-fixed, paraffin-embedded tissue sections. c-kit was observed in 87.7% of SCLC tumors. Using the Kaplan-Mei...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20252
更新日期:2004-08-20 00:00:00
abstract::To investigate whether human melanoma cells intrinsically resistant to autologous LAKs do exist, and whether a relationship between the level of lysis of LAKs and spontaneous drug resistance can be identified at the clonal level, we studied 44 clones obtained from a metastatic melanoma lesion. The antigenic phenotype ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910420412
更新日期:1988-10-15 00:00:00
abstract::We report a population based case-control study of exposure to pesticides as risk factor for non-Hodgkin lymphoma (NHL). Male and female subjects aged 18-74 years living in Sweden were included during December 1, 1999, to April 30, 2002. Controls were selected from the national population registry. Exposure to differe...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23589
更新日期:2008-10-01 00:00:00
abstract::The majority of small-cell lung cancers (SCLCs) express p16 but not pRb. Given our previous study showing loss of pRb in Merkel cell carcinoma (MCC)/neuroendocrine carcinoma of the skin and the clinicopathological similarities between SCLC and MCC, we wished to determine if this was also the case in MCC. Twenty-nine M...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1352
更新日期:2001-08-01 00:00:00
abstract::Eleven population-based cancer registries tabulated second cancers among 133,411 patients diagnosed with testicular cancer, ovarian cancer or Hodgkin's disease between 1945 and 1984. Overall, 3,157 second cancers were observed, as compared with 2,420 expected at least one year after the first cancer. Survivors of test...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390506
更新日期:1987-05-15 00:00:00
abstract::A goal of oncology is to predict chemosensitivity of tumors. This approach assumes that in a patient all tumor deposits are homogeneous. We have tested the heterogeneity between several samples of the same liver metastasis (LM; intrametastatic heterogeneity) or between multiple LM (intermetastatic heterogeneity) from ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25114
更新日期:2010-09-01 00:00:00
abstract::We have surveyed the incidence of adult T-cell leukemia/lymphoma (ATLL) in an endemic area of 290,464 inhabitants for 7 years. We now revise our previous results on the basis of additional findings and estimate the age- and sex-specific cumulative rate for HTLV-I carriers, the adoption of which is recommended by curre...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910430618
更新日期:1989-06-15 00:00:00
abstract::Seventy to 40% of K-RAS wild type colorectal tumors does not seem to benefit from treatment with antiepidermal growth factor receptor (anti-EGFR) monoclonal antibodies. Recent data suggested that in presence of IGF-1 system, altered activation colorectal cancer cells may escape anti-EGFR mediated cell death. The inter...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25193
更新日期:2010-10-15 00:00:00
abstract::T cell responses against malignant cells play a major role in maintaining remission and prolonging overall survival in patients after allogeneic stem cell transplantation and donor lymphocyte infusion (DLI) due to graft-versus-leukemia effect. For better characterization of the T cell responses, we assessed frequency ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31753
更新日期:2019-03-01 00:00:00
abstract::Stimulated by a report on elevated IgA levels in nasopharyngeal carcinoma (NPC), we tested a total of 372 sera from patients with NPC, other carcinomas of head and neck or elsewhere, Burkitt's lymphoma (BL), infectious mononucleosis (IM) or healthy controls. The sera were titrated in indirect immunofluorescence tests ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910170102
更新日期:1976-01-15 00:00:00
abstract::Down-regulation of E-cadherin function is characteristic of cancer cells and might involve the small G-protein Rho family, including Rac1 and Cdc42. IQGAP1 has been reported to be one of the target proteins of Rac1 and Cdc42. To elucidate the role of IQGAP1 in cancer-cell adhesion, its expression was investigated in 4...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(200002)9999:9999<::aid-ijc1121>3
更新日期:2001-03-15 00:00:00
abstract::BRCA1 and BRCA2 play essential roles in maintaining the genome stability. Pathogenic germline mutations in these two genes disrupt their function, lead to genome instability and increase the risk of developing breast and ovarian cancers. BRCA mutations have been extensively screened in Caucasian populations, and the r...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32176
更新日期:2019-08-15 00:00:00
abstract::The promoter variants of TNF-α, a major regulator of immune and inflammation responses, have been implicated in cancer development and prognosis. Thus, we investigated associations between four TNF-α promoter variants and risk of recurrence of squamous cell carcinoma of the oropharynx (SCCOP). We evaluated association...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28512
更新日期:2014-04-15 00:00:00
abstract::Genome-wide association studies have identified more than 80 risk variants for prostate cancer, mainly in European or Asian populations. The generalizability of these variants in other racial/ethnic populations needs to be understood before the loci can be used widely in risk modeling. In our study, we examined 82 pre...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29066
更新日期:2015-03-01 00:00:00
abstract::The Ontario Cancer Registry (OCR) and the Surveillance, Epidemiology and End Results (SEER) databases were used to describe temporal trends in the incidence and survival of squamous cancers of the upper aerodigestive tract (UADT) in Ontario and the US between 1984 and 2001. Between the 1984-86 and 1999-01 periods, the...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24533
更新日期:2009-11-01 00:00:00