The redox protein thioredoxin-1 regulates the constitutive and inducible expression of the estrogen metabolizing cytochromes P450 1B1 and 1A1 in MCF-7 human breast cancer cells.

Abstract:

:The oxidative metabolites of estrogen have been proposed to play an important role in the development of some human cancers. The two major pathways of estrogen metabolism, to the carcinogenic 4-hydroxyestradiol (4-OHE2) and to the non-carcinogenic 2-hydroxyestradiol (2-OHE2), are mediated by cytochromes P450 CYP1B1 and CYP1A1, respectively. The expression of CYP1A1 and CYP1B1 is regulated by the aromatic hydrocarbon receptor/Ah receptor nuclear translocator (AhR/ARNT) transcription factor complex. CYP1B1 expression is elevated in a wide range of human cancers but is not found in corresponding normal tissue. Thioredoxin-1 (Trx-1) is a small redox protein that is overexpressed in a number of human cancers. We report that the expression of CYP1B1 mRNA and protein is increased by Trx-1 transfection of MCF-7 human breast cancer cells and decreased by a redox inactive mutant Trx-1. The Trx-1 inhibitor PX-12 inhibits CYP1B1 gene expression. Trx-1 transfected MCF-7 cells show increased AhR/ARNT DNA binding activity that is not due to altered AhR or ARNT protein expression. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD, dioxin) induced expression of CYP1B1 in MCF-7 cells is increased by Trx-1. Trx-1 does not effect the basal expression of CYP1A1, but increases CYP1A1 mRNA in response to TCDD. The redox inactive mutant Trx-1 completely blocks the induction of both CYP1B1 and CYP1A1 by TCDD. Expression of CYP1A1 but not CYP1B1 has been linked to estrogen receptor (ERalpha) status. Trx-1 transfected MCF-7 cells have decreased ERalpha expression, which may account for the lack of CYP1A1 induction by Trx-1 in the absence of ligand. The results suggest that Trx-1 is involved in the constitutive expression of CYP1B1 and is required for the induction of CYP1B1 and CYP1A1 by TCDD in human MCF-7 breast cancer cells.

journal_name

Carcinogenesis

journal_title

Carcinogenesis

authors

Husbeck B,Powis G

doi

10.1093/carcin/23.10.1625

keywords:

subject

Has Abstract

pub_date

2002-10-01 00:00:00

pages

1625-30

issue

10

eissn

0143-3334

issn

1460-2180

journal_volume

23

pub_type

杂志文章
  • 32P-postlabeling analysis of adducts formed from 1-phenylazo-2-hydroxynaphthalene (Sudan I, Solvent Yellow 14) with DNA and homopolydeoxyribonucleotides.

    abstract::A 32P-postlabeling assay was employed for detection and quantitation of DNA adducts formed with carcinogenic 1-phenylazo-2-hydroxynaphthalene (Sudan I, Solvent Yellow 14) activated by a peroxidase system. Enrichment of adducts by digestion with nuclease P1 or by extraction into n-butanol prior to 32P-labeling was used...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/13.7.1221

    authors: Stiborová M,Frei E,Schmeiser HH,Wiessler M

    更新日期:1992-07-01 00:00:00

  • Fisetin inhibits various attributes of angiogenesis in vitro and in vivo--implications for angioprevention.

    abstract::Studies have shown that fisetin, a small phytochemical molecule, has antitumor activity; however, its antiangiogenic activity has not yet been examined. Accordingly, herein, we investigated the antiangiogenic efficacy and associated mechanisms of fisetin in human umbilical vein endothelial cells (HUVECs). Fisetin (10-...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgr282

    authors: Bhat TA,Nambiar D,Pal A,Agarwal R,Singh RP

    更新日期:2012-02-01 00:00:00

  • DNA polymerase, RNA polymerase and exonuclease activities on a DNA sequence modified by benzo[a]pyrene diolepoxide.

    abstract::Adducts produced by modification of DNA with benzo[a]pyrene diolepoxide (BPDE) are known to inhibit both DNA and RNA synthesis. This phenomenon has been used as a method for determining the distribution of carcinogen binding within defined DNA sequences. A critical comparison of different enzyme activities on adducted...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/13.9.1529

    authors: Thrall BD,Mann DB,Smerdon MJ,Springer DL

    更新日期:1992-09-01 00:00:00

  • Oncogenic transformation of human ovarian surface epithelial cells with defined cellular oncogenes.

    abstract::Ovarian surface epithelium (OSE) is considered to give rise to epithelial ovarian carcinomas (EOCs). To elucidate early processes contributing to the development of EOCs from the OSE, two batches of primary human OSE cells were transduced with non-viral human genes (mutant Cdk4, cyclinD1 and hTERT) so as to efficientl...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgp007

    authors: Sasaki R,Narisawa-Saito M,Yugawa T,Fujita M,Tashiro H,Katabuchi H,Kiyono T

    更新日期:2009-03-01 00:00:00

  • Oltipraz stimulates the transcription of the manganese superoxide dismutase gene in rat hepatocytes.

    abstract::Oltipraz (4-methyl-5-(2-pyrazinyl)-1,2-dithiole-3-thione) (OPZ) is recognized as a potent chemoprotective agent against chemical-induced carcinogenesis in several animal models and is thought to act mainly by inducing phase II conjugating together with inhibiting phase I detoxication enzymes. The present study was und...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/18.11.2113

    authors: Antras-Ferry J,Mahéo K,Chevanne M,Dubos MP,Morel F,Guillouzo A,Cillard P,Cillard J

    更新日期:1997-11-01 00:00:00

  • Selected base excision repair gene polymorphisms and susceptibility to biliary tract cancer and biliary stones: a population-based case-control study in China.

    abstract::Base excision repair (BER) corrects DNA damage caused by oxidative stress and chronic inflammation, putative risk factors for cancer. To understand the relationship between genetic variation in BER genes and risk of biliary tract cancer and biliary stones, we examined non-synonymous polymorphisms in three key BER gene...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgm247

    authors: Huang WY,Gao YT,Rashid A,Sakoda LC,Deng J,Shen MC,Wang BS,Han TQ,Zhang BH,Chen BE,Rosenberg PS,Chanock SJ,Hsing AW

    更新日期:2008-01-01 00:00:00

  • Antioxidant genes, diabetes and dietary antioxidants in association with risk of pancreatic cancer.

    abstract::To test the hypothesis that polymorphic variants of antioxidant genes modify the risk of pancreatic cancer, we examined seven single-nucleotide polymorphisms (SNPs) of genes coding for superoxide dismutase (SOD) 2, glutathione S-transferase alpha 4 (GSTA4), catalase and glutathione peroxidase in 575 patients with panc...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgp310

    authors: Tang H,Dong X,Day RS,Hassan MM,Li D

    更新日期:2010-04-01 00:00:00

  • Metabolic reprogramming and dysregulated metabolism: cause, consequence and/or enabler of environmental carcinogenesis?

    abstract::Environmental contributions to cancer development are widely accepted, but only a fraction of all pertinent exposures have probably been identified. Traditional toxicological approaches to the problem have largely focused on the effects of individual agents at singular endpoints. As such, they have incompletely addres...

    journal_title:Carcinogenesis

    pub_type: 杂志文章,评审

    doi:10.1093/carcin/bgv037

    authors: Robey RB,Weisz J,Kuemmerle NB,Salzberg AC,Berg A,Brown DG,Kubik L,Palorini R,Al-Mulla F,Al-Temaimi R,Colacci A,Mondello C,Raju J,Woodrick J,Scovassi AI,Singh N,Vaccari M,Roy R,Forte S,Memeo L,Salem HK,Amedei A,

    更新日期:2015-06-01 00:00:00

  • Over-expression of EphB3 enhances cell-cell contacts and suppresses tumor growth in HT-29 human colon cancer cells.

    abstract::Receptor tyrosine kinase EphB3 is expressed in cells in the bottom of intestinal crypts near stem cell niches. Loss of Ephb3 has recently been reported to produce invasive colorectal carcinoma in Apc(Min/+) mice and EphB-mediated compartmentalization was demonstrated to be a mechanism suppressing colorectal cancer pro...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgp133

    authors: Chiu ST,Chang KJ,Ting CH,Shen HC,Li H,Hsieh FJ

    更新日期:2009-09-01 00:00:00

  • The role of P-glycoprotein in intestinal tumorigenesis: disruption of mdr1a suppresses polyp formation in Apc(Min/+) mice.

    abstract::P-glycoprotein (P-gp) mediates the active transport of various substrates including xenobiotics, and it thus has a protective function in various cell types and tissues/organs including the intestinal epithelium. However, whether or not P-gp plays a positive role in the intestinal tumorigenesis is unclear. We have int...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgg073

    authors: Mochida Y,Taguchi K,Taniguchi S,Tsuneyoshi M,Kuwano H,Tsuzuki T,Kuwano M,Wada M

    更新日期:2003-07-01 00:00:00

  • Membrane-type matrix metalloproteinases mediate curcumin-induced cell migration in non-tumorigenic colon epithelial cells differing in Apc genotype.

    abstract::Colonic epithelial cell migration is required for normal differentiated cell function. This migratory phenotype is dependent upon wild-type adenomatous polyposis coli (Apc) expression. Non-tumorigenic murine colon epithelial cell lines with distinct Apc genotypes, i.e. young adult mouse colon (YAMC; Apc(+/+)) and immo...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/23.6.1065

    authors: Fenton JI,Wolff MS,Orth MW,Hord NG

    更新日期:2002-06-01 00:00:00

  • Enhancement of dimethylnitrosamine-initiated hepatocarcinogenesis in hamsters by subsequent administration of carbon tetrachloride but not phenobarbital or p,p'-dichlorodiphenyltrichloroethane.

    abstract::The effect of phenobarbital (PB), p,p'-dichlorodiphenyltrichloroethane (DDT) or carbon tetrachloride (CCl4) on dimethylnitrosamine (DMN)-induced hepatocarcinogenesis in Syrian golden hamsters was examined. Hamsters were given a single injection of DMN (6 mg/kg body wt) followed by either PB or DDT in the diet or repea...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/8.9.1171

    authors: Tanaka T,Mori H,Williams GM

    更新日期:1987-09-01 00:00:00

  • Formation of N-7-(2-carbamoyl-2-hydroxyethyl)guanine in DNA of the mouse and the rat following intraperitoneal administration of [14C]acrylamide.

    abstract::Acrylamide is an alkylating agent which reacts very slowly in direct reactions with DNA and is negative in the Ames test, but is carcinogenic in mice and rats. In order to explain the cancer-initiating properties of acrylamide we have studied DNA adduct formation in vitro with a metabolizing system and in vivo in mice...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/16.5.1161

    authors: Segerbäck D,Calleman CJ,Schroeder JL,Costa LG,Faustman EM

    更新日期:1995-05-01 00:00:00

  • Carcinogen metabolism in cultured human tissues and cells.

    abstract::A large number of chemical carcinogens require metabolic activation before they are biologically active. The metabolism of a few of these compounds has been investigated in cultured human tissues and cells and the metabolism has been compared with the metabolism in organs from experimental animals in which the compoun...

    journal_title:Carcinogenesis

    pub_type: 杂志文章,评审

    doi:10.1093/carcin/11.5.707

    authors: Autrup H

    更新日期:1990-05-01 00:00:00

  • The metabolism and activation of polycyclic aromatic hydrocarbons in epithelial cell aggregates and fibroblasts prepared from rat mammary tissue.

    abstract::The metabolism of 7,12-dimethylbenz[a]anthracene (DMBA), benzo[a]pyrene (BP) and benz[a]anthracene (BA) by epithelial cell aggregates and fibroblasts in culture has been investigated using mammary tissue obtained from female Wistar rats. The results show that: (a) both types of mammary cells metabolise all three hydro...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/3.2.203

    authors: Cooper CS,Pal K,Hewer A,Grover PL,Sims P

    更新日期:1982-01-01 00:00:00

  • Studies on the mechanism by which a tumor promoter inhibits binding of epidermal growth factor to cellular receptors.

    abstract::This study analyzes the mechanism by which the tumor promoter 12-0-tetradecanoyl-phorbol 13-acetate (TPA) inhibits binding of epidermal growth factor (EGF) to its membrane receptors in HeLa cells. Kinetic studies indicate that inhibition of EGF binding occurs within a few minutes after exposure of cells to TPA; delaye...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/1.8.669

    authors: Lee LS,Weinstein IB

    更新日期:1980-08-01 00:00:00

  • Hepatitis B virus X protein enhances the development of liver fibrosis and the expression of genes associated with epithelial-mesenchymal transitions and tumor progenitor cells.

    abstract::The hepatitis B virus X protein (HBx) has pleiotropic biological effects, which underlies its potential role in cell transformation. However, its involvement in hepatic fibrosis remains unclear. In this study, we wanted to clarify, in vivo, the role of HBx protein in the development of liver fibrosis. Mice transgenic ...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgz109

    authors: Ahodantin J,Lekbaby B,Bou Nader M,Soussan P,Kremsdorf D

    更新日期:2020-05-14 00:00:00

  • Genetic variations in the transforming growth factor-beta pathway as predictors of survival in advanced non-small cell lung cancer.

    abstract::The magnitude of benefit is variable for advanced non-small cell lung cancer (NSCLC) patients receiving platinum-based chemotherapy. The purpose of this study is to determine whether genetic variations in the transforming growth factor-beta (TGF-β) pathway are associated with clinical outcomes in NSCLC patients receiv...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgr067

    authors: Lin M,Stewart DJ,Spitz MR,Hildebrandt MA,Lu C,Lin J,Gu J,Huang M,Lippman SM,Wu X

    更新日期:2011-07-01 00:00:00

  • v-sis oncogene-induced transformation of human fibroblasts into cells capable of forming benign tumors.

    abstract::Normal human fibroblasts do not synthesize platelet-derived growth factor (PDGF), but they respond to its mitogenic action. In contrast, cells from several human fibrosarcomas have been shown to synthesize PDGF at significant levels. To investigate the possible role of PDGF expression in the development of tumors of m...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/15.10.2167

    authors: Yang D,Kohler SK,Maher VM,McCormick JJ

    更新日期:1994-10-01 00:00:00

  • Composition and turnover of phospholipids and neutral lipids in human breast cancer and reference tissues.

    abstract::The phospholipid (PL) content was 4-fold higher while the triacylglycerol (TG) content tended to be 65% lower in human breast cancer tissues as compared with non-cancerous reference parts from excised breast tissues. The variation in TG content among breast tissues was very large while that of PL was relatively small....

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/13.4.579

    authors: Sakai K,Okuyama H,Yura J,Takeyama H,Shinagawa N,Tsuruga N,Kato K,Miura K,Kawase K,Tsujimura T

    更新日期:1992-04-01 00:00:00

  • In vivo mutagenesis of the reporter plasmid pSP189 induced by exposure of host Ad293 cells to activated polymorphonuclear leukocytes.

    abstract::We measured the mutation frequency and spectrum induced by exposure of the mutation reporter plasmid pSP189 in vivo to phorbol myristate acetate (PMA)-activated human polymorphonuclear leukocytes (PMNs). The mutation frequency induced in the supF tRNA gene of pSP189 transfected into human Ad293 cells by a 30 min expos...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/17.10.2137

    authors: Akman SA,Sander F,Garbutt K

    更新日期:1996-10-01 00:00:00

  • AFF4 promotes tumorigenesis and tumor-initiation capacity of head and neck squamous cell carcinoma cells by regulating SOX2.

    abstract::Super elongation complex (SEC) controls gene transcription by releasing Pol II from pausing. Previous studies have shown that dysfunction of SEC was associated with multiple human cancers, such as leukemia and breast cancer. However, the role of SEC in head and neck squamous cell carcinoma (HNSCC) development remains ...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgy046

    authors: Deng P,Wang J,Zhang X,Wu X,Ji N,Li J,Zhou M,Jiang L,Zeng X,Chen Q

    更新日期:2018-07-03 00:00:00

  • Focal and non-focal hepatic expression of placental glutathione S-transferase in carcinogen-treated rats.

    abstract::Carcinogenesis develops in stages that have been operationally defined as initiation, promotion and progression. Although morphological end points have been described for detection and quantitation of these stages, to date initiation has been assessed only in the context of clonal growth in response to certain promoti...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/15.11.2587

    authors: Dragan YP,Campbell HA,Baker K,Vaughan J,Mass M,Pitot HC

    更新日期:1994-11-01 00:00:00

  • Anti-HPV-E7 seropositivity and risk of esophageal squamous cell carcinoma in a high-risk population in China.

    abstract::Results of previous serologic studies on the association of human papillomavirus (HPV) with esophageal squamous cell carcinoma (ESCC) have been inconsistent. From 2007 to 2010, the authors collected blood samples and relevant demographic data from 1435 patients with ESCC and 2071 age- and sex-matched normal controls f...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgt483

    authors: He Z,Xu Z,Hang D,Guo F,Abliz A,Weiss NS,Xi L,Liu F,Ning T,Pan Y,Guo C,Liang Y,Lu C,Zhang L,Cai H,Ke Y

    更新日期:2014-04-01 00:00:00

  • Prepubertal estradiol and genistein exposures up-regulate BRCA1 mRNA and reduce mammary tumorigenesis.

    abstract::Prepubertal exposure to soy or its biologically active component genistein reduces later breast cancer risk in both animal models and human populations. We investigated whether that might be due to reported estrogenic properties of genistein. Our study indicated that daily prepubertal exposures between postnatal days ...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgh065

    authors: Cabanes A,Wang M,Olivo S,DeAssis S,Gustafsson JA,Khan G,Hilakivi-Clarke L

    更新日期:2004-05-01 00:00:00

  • Suppression of 3-methylcholanthrene-induced cellular transformation by timed administration of the Bowman-Birk protease inhibitor.

    abstract::The Bowman-Birk protease inhibitor (BBI) is a legume-derived inhibitor of chymotrypsin and trypsin that has been shown to suppress cellular transformation and tumorigenesis. In the present investigation the effects of various BBI administration schedules were evaluated for suppression of 3-methylcholanthrene (3-MCA)-i...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/12.5.935

    authors: St Clair WH

    更新日期:1991-05-01 00:00:00

  • Inhibition of neoplastic development in rat liver, kidney, oesophagus and forestomach by 4,4'-diaminodiphenylmethane administration.

    abstract::The modifying effects of 4,4'-diaminodiphenylmethane (DDPM) (0.1% in diet) administration on liver carcinogenesis induced by 2-acetylaminofluorene (2-AAF) (0.02% in diet), 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) ( 0.06% in diet), diethylnitrosamine (DEN) (0.001% in drinking water) and N-ethyl-N-hydroxyethylnit...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/8.5.719

    authors: Tsuda H,Ogiso T,Hasegawa R,Imaida K,Masui T,Ito N

    更新日期:1987-05-01 00:00:00

  • Proteomic analysis of the OGT interactome: novel links to epithelial-mesenchymal transition and metastasis of cervical cancer.

    abstract::The role of O-GlcNAc transferase (OGT) in gene regulation and tumor invasion is poorly understood. Here, we have identified several previously undiscovered OGT-interacting proteins, including the PRMT5/WDR77 complex, the PRC2 complex, the ten-eleven translocation (TET) family, the CRL4B complex and the nucleosome remo...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgy097

    authors: Gao J,Yang Y,Qiu R,Zhang K,Teng X,Liu R,Wang Y

    更新日期:2018-10-08 00:00:00

  • GSTM1 and CYP2D6 genotype frequencies in patients with pituitary tumours: effects on P53, ras and gsp.

    abstract::We describe studies to determine if susceptibility to pituitary tumours is associated with the putatively high risk GSTM1 null and CYP2D6 EM genotypes. Frequency distributions of these genotypes were similar in cases and controls though the frequency of CYP2D6 PM and GSTM1 B tended to be lower (P = 0.072 and P = 0.095...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/16.7.1643

    authors: Perrett CW,Clayton RN,Pistorello M,Boscaro M,Scanarini M,Bates AS,Buckley N,Jones P,Fryer AA,Gilford J

    更新日期:1995-07-01 00:00:00

  • Diffuse-type gastric cancer cells switch their driver pathways from FGFR2 signaling to SDF1/CXCR4 axis in hypoxic tumor microenvironments.

    abstract::Cancer-associated fibroblasts (CAFs) have been considered to play an important role for tumor progression of cancer. Solid tumors contain heterogeneous distribution of oxygen in their microenvironments. This study investigated the growth signaling of gastric cancer (GC) cells in focus on the interaction with CAFs and ...

    journal_title:Carcinogenesis

    pub_type: 杂志文章

    doi:10.1093/carcin/bgv134

    authors: Kinoshita H,Yashiro M,Fukuoka T,Hasegawa T,Morisaki T,Kasashima H,Masuda G,Noda S,Hirakawa K

    更新日期:2015-12-01 00:00:00