Disruption of dog-1 in Caenorhabditis elegans triggers deletions upstream of guanine-rich DNA.

Abstract:

:Genetic integrity is crucial to normal cell function, and mutations in genes required for DNA replication and repair underlie various forms of genetic instability and disease, including cancer. One structural feature of intact genomes is runs of homopolymeric dC/dG. Here we describe an unusual mutator phenotype in Caenorhabditis elegans characterized by deletions that start around the 3' end of polyguanine tracts and terminate at variable positions 5' from such tracts. We observed deletions throughout genomic DNA in about half of polyguanine tracts examined, especially those containing 22 or more consecutive guanine nucleotides. The mutator phenotype results from disruption of the predicted gene F33H2.1, which encodes a protein with characteristics of a DEAH helicase and which we have named dog-1 (for deletions of guanine-rich DNA). Nematodes mutated in dog-1 showed germline as well as somatic deletions in genes containing polyguanine tracts, such as vab-1. We propose that DOG-1 is required to resolve the secondary structures of guanine-rich DNA that occasionally form during lagging-strand DNA synthesis.

journal_name

Nat Genet

journal_title

Nature genetics

authors

Cheung I,Schertzer M,Rose A,Lansdorp PM

doi

10.1038/ng928

keywords:

subject

Has Abstract

pub_date

2002-08-01 00:00:00

pages

405-9

issue

4

eissn

1061-4036

issn

1546-1718

pii

ng928

journal_volume

31

pub_type

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