Abstract:
:Enterovirus infections of the central nervous system (CNS) are common and important causes of morbidity in immunocompromised children and adults. In this study we identify and characterize coxsackievirus B4-induced neuronal death. To investigate the CNS pathophysiology resulting from this viral infection, cultured rat neurons were infected with coxsackievirus B4 (CVB4) and nuclear morphology, phosphatidylserine (PS) externalization, and the effects of Actinomycin D or cycloheximide (CHX) were examined. CVB4 induced neuronal cell death within 24 h while PS externalization was apparent in cell bodies 16 h after CVB4 infection. Actinomycin D or CHX significantly reduced CVB4 induced-neuronal cell death in a dose-dependent manner. Pretreatment with CHX or actinomycin D also inhibited nuclear condensation, which occurred after CVB4 infection. However, the changes were relatively unresponsive to zVAD-fmk. These results suggest that CVB4 induces CHX- and actinomycin D-sensitive, but zVAD-fmk-insensitive neuronal apoptosis.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Joo CH,Kim YK,Lee H,Hong H,Yoon SY,Kim Ddoi
10.1016/s0304-3940(02)00340-3keywords:
subject
Has Abstractpub_date
2002-07-05 00:00:00pages
175-8issue
3eissn
0304-3940issn
1872-7972pii
S0304394002003403journal_volume
326pub_type
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