Abstract：
OBJECTIVE:To use microarray analysis as an unbiased approach to identify genes involved in the induction and growth of uterine leiomyomata. DESIGN:Screen by arrays for up to 12,000 genes in leiomyoma (L) and control myometrium (M) from nine patients. SETTING:University research laboratories. PATIENT(S):Nine patients in the follicular and luteal phases of the menstrual cycle. INTERVENTION(S):mRNA from L and M was converted to biotin-labeled cRNA and hybridized to cDNA oligonucleotide sequences on the arrays. MAIN OUTCOME MEASURE(S):Greater than two-fold change in gene expression between leiomyoma and matched myometrium. RESULT(S):Prominent among the 67 genes overexpressed in L relative to M were dlk or Pref-1, doublecortin, JM27, ionotropic glutamate receptor subunit 2, apolipoprotein E3, IGF2, semaphorin F, myelin proteolipid protein, MEST, frizzled, CRABP II, stromelysin-3, and TGFbeta3. The genes dlk, IGF2, and MEST are paternally expressed imprinted genes, and the others are involved in tissue differentiation and growth. Prominent among the 78 genes down-regulated in L relative to M were alcohol dehydrogenases 1alpha-gamma, tryptase, dermatopontin, thrombospondin, coxsackievirus receptor, nur77, and c-kit. CONCLUSION(S):Arrays offer large-scale screening of mRNA expression, which will help us differentiate between the genes and metabolic pathways necessary for leiomyoma growth and those regulating myometrial contractions.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Tsibris JC,Segars J,Coppola D,Mane S,Wilbanks GD,O'Brien WF,Spellacy WNdoi
10.1016/s0015-0282(02)03191-6keywords:
subject
Has Abstractpub_date
2002-07-01 00:00:00pages
114-21issue
1eissn
0015-0282issn
1556-5653pii
S0015028202031916journal_volume
78pub_type
杂志文章abstract：OBJECTIVE:To evaluate the effectiveness of hysteroscopic selective salpingography (HSS) as a method for diagnosing the tubal proximal occlusion shown by hysterosalpingography (HSG). DESIGN:Prospective study. SETTING:Outpatient Department of Obstetrics and Gynecology, Social Insurance Saitama Chuo Hospital, Urawa, Jap...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00324-6
更新日期：1999-10-01 00:00:00
abstract：:A couple may be considered to have fertility problems if they have been trying to conceive for over 1 year with no success. Worldwide, the inability to have children affects 10% to 15% of all couples. Subfertility can be divided into either male or female factor, or both partners can be affected. However, for some cou...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2018.05.028
更新日期：2018-09-01 00:00:00
abstract：:The outcome of a series of pre-embryo transfers to 31 women with ovarian failure is described. Twenty six fertile women functioned as nonanonymous donors, providing oocytes for in vitro fertilization after undergoing controlled ovarian hyperstimulation and transvaginal ultrasound directed oocyte aspiration. Recipients...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54055-2
更新日期：1991-01-01 00:00:00
abstract：:A highly sensitive 2-hour tube test for the detection of human chorionic gonadotropin (hCG) in urine was compared with the more resource-demanding radioimmunoassay for hCG in serum as an aid in the diagnosis of ectopic pregnancy. In a first series of 107 patients with the possibility of ectopic pregnancy, the urine te...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48559-6
更新日期：1985-05-01 00:00:00
abstract：:Combined therapy with clomiphene and intranasal luteinizing hormone-releasing hormone (LHRH) was used to induce ovulation in eight chronically anovulatory patients under treatment for infertility. Clomiphene, 100 mg daily, was given from the 5th to the 9th day of the cycle. Synthetic LHRH was administered intranasally...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1980-11-01 00:00:00
abstract：OBJECTIVE:To determine if increases in core body temperature preceding most hot flashes are caused by increased metabolic rate, peripheral vasoconstriction, or central noradrenergic activation. DESIGN:Laboratory physiological study. SETTING:University medical center. PATIENT(S):Fourteen healthy, postmenopausal women...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(98)00137-x
更新日期：1998-08-01 00:00:00
abstract：:We found, by reverse transcription--real time--polymerase chain reaction, that the expression of aromatase (CYP19) in ovarian, peritoneal endometriosis, and deep endometriotic nodules is significantly different, which strengthens the theory of three distinct clinical entities. Compared with peritoneal endometriosis, o...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2005.10.041
更新日期：2006-05-01 00:00:00
abstract：OBJECTIVE:To assess the expression of the oxytocin receptor (OTR) and the role of oxytocin (OT) in the proliferation of myometrial and leiomyoma cells. DESIGN:Prospective laboratory study. SETTING:Research laboratory at the Italian National Research Council. PATIENT(S):Twenty-two women who underwent therapeutic myom...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.10.064
更新日期：2010-10-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of transforming growth factor (TGF)-beta1 on the extracellular signal-regulated kinase (ERK) and Smad pathway and the role of peroxisome proliferator-activated receptor (PPAR)-gamma in cultured human endometrial stromal cells. DESIGN:Experimental study. SETTING:Infertility center o...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.09.010
更新日期：2008-10-01 00:00:00
abstract：OBJECTIVE:To investigate the direct actions of active 1,25-dihydroxy vitamin D3 (VD3) upon primate follicular development at specific stages of folliculogenesis. DESIGN:Secondary preantral follicles were isolated from rhesus monkeys ovaries, encapsulated in alginate, and cultured for 40 days. Follicles were randomly a...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2016.08.037
更新日期：2016-12-01 00:00:00
abstract：OBJECTIVE:To determine intrafollicular hormone levels and characterize the mRNA expression of the insulin-like growth factor (IGF) receptors, IGF binding proteins (IGFBP), and pregnancy-associated plasma protein-A (PAPP-A) in granulosa cells before and after an ovulatory hCG stimulus. DESIGN:Experimental animal study....
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.12.096
更新日期：2010-03-15 00:00:00
abstract：OBJECTIVE:To review the mechanisms of T replacement therapy's inhibition of spermatogenesis and current therapeutic approaches in reproductive aged men. DESIGN:Review of published literature. SETTING:PubMed search from 1990-2012. PATIENT(S):PubMed search from 1990-2012. INTERVENTION(S):A literature review was perfo...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2012.10.052
更新日期：2013-03-01 00:00:00
abstract：:The negative correlation between the thickness of the lamina propria and Johnsen's score count in individual seminiferous tubules was observed and the tubules with thin lamina propria indicated almost complete spermatogenesis. It is suggested that the thin lamina propria may be one of the good indicators of complete s...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.10.019
更新日期：2008-10-01 00:00:00
abstract：OBJECTIVE:To determine if adult human endometrium possesses an intact müllerian-inhibiting substance (MIS) signal transduction system and, if so, whether MIS can modulate endometrial cell viability. DESIGN:Adult human endometrial tissue was subjected to reverse transcription polymerase chain reaction (RT-PCR) analysis...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.01.028
更新日期：2009-04-01 00:00:00
abstract：:In this study, both IVF-ET cycle and pregnancy outcome were compared with increases seen in P concentration during the 24-hour period from 12 hours before to 12 hours after hCG administration. A significantly higher PR (P < 0.001) was seen in women who had at least a threefold increase in P during this time period. Fu...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：OBJECTIVE:To evaluate the place of ultrasound-directed transvaginal transmyometrial ET in a protocol using both the transcervical and transmyometrial routes in a step-wise fashion. DESIGN:A prospective descriptive clinical study. SETTING:A university-based assisted conception unit. PATIENTS:Thirteen patients who had...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：:The use of 10-cm-long plastic tuberculin syringes for freezing and storage of human semen is introduced. This new method reduces the number of prefreezing and postthawing manipulations to a minimum. Postthawing motility, penetration of spermatozoa into cervical mucus, and results of the hamster ovum test were similar ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)59175-4
更新日期：1987-05-01 00:00:00
abstract：OBJECTIVE:To determine the incidence and type of mixoploidy in human blastocysts produced in vitro. DESIGN:A laboratory study of spare blastocysts from an IVF program. SETTING:University hospital laboratory. PATIENT(S):Thirty-nine couples undergoing IVF or intracytoplasmic sperm injection. INTERVENTION(S):A total o...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)04393-5
更新日期：2002-12-01 00:00:00
abstract：OBJECTIVE:To determine the meiotic behavior of a ring Y chromosome in a semen sample from a 45,X/46,X,r(Y)/46,X,dic r(Y) patient and the possible interchromosomal effects of the ring on other chromosome pairs. DESIGN:Retrospective analysis. SETTING:Universitat Autònoma de Barcelona. PATIENT:An oligoasthenoteratozoos...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)04931-2
更新日期：2003-04-01 00:00:00
abstract：OBJECTIVE:To assess the association of total isoflavone intake with ovulatory function, including sporadic anovulation in healthy premenopausal women. DESIGN:Prospective cohort study. SETTING:University. PATIENT(S):Participants included 259 healthy regularly menstruating women aged 18-44 years. INTERVENTION(S):None...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.08.002
更新日期：2013-12-01 00:00:00
abstract：OBJECTIVE:To assess public attitudes toward placing age limits on men and women seeking fertility treatment. DESIGN:Cross-sectional web-based survey. SETTING:Not applicable. PATIENT(S):A nationally representative sample of 1,427 adults in the United States. INTERVENTION(S):Not applicable. MAIN OUTCOME MEASURE(S):S...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2018.11.019
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:To determine optimal management with one or two mature follicles after stimulation. DESIGN:Retrospective analysis. SETTING:Lister fertility clinic. PATIENT(S):A total of 1,350 IVF/intracytoplasmic sperm injection cycles (7.3% of total) during 1998-2009 were found to have one or two mature follicles. INTER...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.05.030
更新日期：2011-01-01 00:00:00
abstract：:The beta-adrenergic agonist isoproterenol was infused intravenously for 4 hours during the midluteal phase of the cycle in six normal women. A rapid increase in cardiac output occurred for the entire duration of the isoproterenol infusion and serum estrogen and progesterone levels (but not luteinizing hormone levels) ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)47712-5
更新日期：1984-03-01 00:00:00
abstract：OBJECTIVE:To identify and characterize a novel sperm motility-stimulating protein (MSP) from caprine serum. DESIGN:Prospective experimental study. SETTING:Research laboratory. ANIMAL(S):Rabbits and male and female BALB/c mice. INTERVENTION(S):Protein purification by conventional methods and functional and immunolog...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.03.012
更新日期：2013-07-01 00:00:00
abstract：OBJECTIVE:To investigate differentially expressed genes in epithelial and stromal cells of eutopic endometrium from patients with deep endometriosis and women with normal pelvic cavities using laser capture microdissection and complementary DNA microarrays. DESIGN:Prospective study. SETTING:University hospital. PATI...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.04.041
更新日期：2005-10-01 00:00:00
abstract：:A retrospective review of 237 initial, fresh nondonor IVF cycles in which all embryos generated during the cycle were transferred on either day 2 (n = 109) or day 3 (n = 128) were evaluated with regards to reproductive outcomes. Patients who underwent a day 2 ET had similar conception (18% vs. 16%; relative risk [RR],...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.10.010
更新日期：2011-03-01 00:00:00
abstract：OBJECTIVE:To validate recently proposed models of ovarian nongrowing follicle (NGF) decay associated with aging within the context of an independent data set. DESIGN:Prospective investigation. SETTING:Academic medical center. PATIENT(S):Normal appearing ovaries collected from 52 women (age 28-51 years) undergoing oo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.12.008
更新日期：2014-03-01 00:00:00
abstract：OBJECTIVE:To determine the expression of nitric oxide synthases (NOSs) and their modulation by hypoxia in human peritoneal (NF) and adhesion fibroblasts (ADF). DESIGN:Prospective experimental study. SETTING:University medical center. PATIENT(S):Fibroblasts from peritoneum and adhesion tissues. INTERVENTION(S):Hypox...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.07.1313
更新日期：2008-09-01 00:00:00
abstract：OBJECTIVE:To determine the incidence of spontaneous pregnancy in women who were not actively undergoing therapy after a successful attempt at IVF/intracytoplasmic sperm injection and to characterize its pattern of occurrence. DESIGN:Retrospective postal questionnaire. SETTING:An assisted reproduction unit at a univer...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00609-3
更新日期：2000-04-01 00:00:00
abstract：OBJECTIVE:To determine the rearrangement of mitochondrial DNA (mtDNA) in unfertilized human oocytes and compromised embryos to evaluate the fertilization capacity of oocytes. DESIGN:Prospective laboratory research. SETTING:IVF laboratory in a university hospital. PATIENT(S):One hundred twenty-four unfertilized oocyt...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)02994-1
更新日期：2002-05-01 00:00:00
