Epitope mapping of mitochondrial adenine nucleotide translocase-1 in idiopathic dilated cardiomyopathy.


:Mitochondrial adenine nucleotide translocase (ANT) is a specific target for the autoantibody response in idiopathic dilated cardiomyopathy (IDCM). We have undertaken an epitope analysis of ANT in IDCM by immunoblot with recombinant GST-ANT fusion proteins and with cellulose-bound decapeptides of human ANT1. Forty-five patients with IDCM, 17 patients with ischemic left ventricle dysfunction (LVD) and 20 controls were analyzed for circulating antibodies against ANT (AAb-ANT). Sixteen of the 45 (36%) IDCM patients showed AAb-ANT above controls. In immunoblots, AAb-ANT detected purified bovine heart ANT and GST-ANT1 and GST-ANT2 isoforms and, less frequently, the GST-ANT3 isoform. A construct lacking the last 146 amino acids did not react with AAb-ANT, indicating that the main epitopes are in the C-terminal 146 amino acids. Immunodetection of decapeptides covering this region shows that AAb-ANT detects at least three epitopes, demonstrating that ANT is the primary target of AAb-ANT. The most significant epitopes belong to the M2 and M3 hydrophilic loops of ANT suggesting that apart from being essential for its activity, these loops are highly immunogenic.


J Mol Cell Cardiol


Manchado C,Orús J,Villarroya F,Roig E,Heras M,Giralt M,Iglesias R,Sanz G,Mampel T,Viñas O





Has Abstract


2002-05-01 00:00:00














  • Dual action of prajmalium on the Ca currents in frog isolated cardiomyocytes.

    abstract::The effects of N-n-propylajmaline (prajmalium) on the Na and Ca currents of single frog atrial and ventricular cells were studied by means of the whole-cell patch-clamp technique. Prajmalium (10(-9) to 10(-6) M) depressed the Na current (INa) in a dose- and use-dependent manner. In the same range of concentrations, pr...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Alvarez JL,Vassort G

    更新日期:1991-05-01 00:00:00

  • Polyamines mediate coronary transcapillary macromolecular transport in the calcium paradox.

    abstract::This study addresses the role of polyamines and their rate limiting enzyme, ornithine decarboxylase in regulation of macromolecular transport of two macromolecules, fluorescein and horseradish peroxidase, across coronary capillaries. Rat hearts were isolated and retrogradely perfused through the aorta (Langendorff met...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Trout JJ,Lu CY,Goldstone AD,Iqbal Z,Koenig H

    更新日期:1994-03-01 00:00:00

  • Inhibitory activity of antibodies against the human atrial 5-HT(4)receptor.

    abstract::Antibodies directed against the second extracellular loop of G protein-coupled receptors have been shown to exert "agonist-like" activities. In order to test the hypothesis that this is a general phenomenon, antibodies were raised in rabbits against a synthetic peptide corresponding to the second extracellular loop of...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Sallé L,Eftekhari P,Aupart M,Cosnay P,Hoebeke J,Argibay JA

    更新日期:2001-03-01 00:00:00

  • 'Calcium paradox' in the heart is modulated by cell sodium during the calcium-free period.

    abstract::We hypothesized that after a Ca2+-free period the magnitude of the Na+ gradient at the onset of Ca2+ reperfusion would grade the ensuing cell Ca2+ gain. Rabbit interventricular septa perfused with Hepes buffered solution (pH 7.4, [Ca2+] = 1.0 mM) and stimulated to contract isometrically at 60 min-1 at 30 degrees C wer...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Ruaño-Arroyo G,Gerstenblith G,Lakatta EG

    更新日期:1984-09-01 00:00:00

  • Sarcoplasmic reticulum Ca2+, Mg2+, K+, and Cl- concentrations adjust quickly as heart rate changes.

    abstract::During systole, Ca2+ is released from the sarcoplasmic reticulum (SR) through ryanodine receptors (RyRs) while, simultaneously, other ions (specifically K+, Mg2+, and Cl-) provide counter-ion flux. These ions move back into the SR during diastole through the SERCA pump and SR K+ and Cl- channels. In homeostasis, all i...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Berti C,Zsolnay V,Shannon TR,Fill M,Gillespie D

    更新日期:2017-02-01 00:00:00

  • Targeting cardiac fibroblasts to treat fibrosis of the heart: focus on HDACs.

    abstract::Cardiac fibrosis is implicated in numerous physiologic and pathologic conditions, including scar formation, heart failure and cardiac arrhythmias. However the specific cells and signaling pathways mediating this process are poorly understood. Lysine acetylation of nucleosomal histone tails is an important mechanism fo...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Schuetze KB,McKinsey TA,Long CS

    更新日期:2014-05-01 00:00:00

  • Myocardium-targeted delivery of endothelial progenitor cells by ultrasound-mediated microbubble destruction improves cardiac function via an angiogenic response.

    abstract::Application of ultrasound-mediated destruction of microbubbles (US + Bubble) to skeletal muscle creates capillary ruptures leading to leakage of the cell components. We studied whether US + Bubble combined with bone-marrow-derived mononuclear cells (BM-MNCs) infusion enables the targeted delivery of endothelial-lineag...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Zen K,Okigaki M,Hosokawa Y,Adachi Y,Nozawa Y,Takamiya M,Tatsumi T,Urao N,Tateishi K,Takahashi T,Matsubara H

    更新日期:2006-06-01 00:00:00

  • Peroxynitrite activates ERK via Raf-1 and MEK, independently from EGF receptor and p21Ras in H9C2 cardiomyocytes.

    abstract::Peroxynitrite is a potent oxidant and nitrating species proposed as a direct effector of myocardial damage in a wide range of cardiac diseases. Whether peroxynitrite also acts indirectly, by modulating cell signal transduction pathways in the myocardium, has not been investigated. Here, we examined the ability of pero...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Pesse B,Levrand S,Feihl F,Waeber B,Gavillet B,Pacher P,Liaudet L

    更新日期:2005-05-01 00:00:00

  • Extracellular matrix alterations in hypertensive vascular remodeling.

    abstract::Vascular cells are very sensitive to their hemodynamic environment. Any change in blood pressure or blood flow can be sensed by endothelial and vascular smooth muscle cells and ultimately results in structural modifications within the vascular wall that accommodate the new conditions. In the case of hypertension, the ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Lemarié CA,Tharaux PL,Lehoux S

    更新日期:2010-03-01 00:00:00

  • Sarcoplasmic reticulum Ca(2+)ATPase and cell contraction in developing rabbit heart.

    abstract::The purpose of the present study was to determine whether age-related changes in the expression and function of the cardiac isoform of the sarcoplasmic reticulum Ca(2+)-ATPase (SERCA2a) play a role in SR Ca(2+)release and cell contraction. SERCA2a protein levels and subcellular localization were compared between fetal...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Chen F,Ding S,Lee BS,Wetzel GT

    更新日期:2000-05-01 00:00:00

  • Gender influences on sarcoplasmic reticulum Ca2+-handling in failing human myocardium.

    abstract::Gender has recently been implicated as an important modulator of cardiovascular disease. However, it is not known how gender may specifically influence the Ca2+-handling deficits that characterize the depressed cardiac contractility of human heart failure. To elucidate the contributory role of gender to sarcoplasmic r...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Dash R,Frank KF,Carr AN,Moravec CS,Kranias EG

    更新日期:2001-07-01 00:00:00

  • Conditional knockout of Fgf13 in murine hearts increases arrhythmia susceptibility and reveals novel ion channel modulatory roles.

    abstract::The intracellular fibroblast growth factors (iFGF/FHFs) bind directly to cardiac voltage gated Na+ channels, and modulate their function. Mutations that affect iFGF/FHF-Na+ channel interaction are associated with arrhythmia syndromes. Although suspected to modulate other ionic currents, such as Ca2+ channels based on ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Wang X,Tang H,Wei EQ,Wang Z,Yang J,Yang R,Wang S,Zhang Y,Pitt GS,Zhang H,Wang C

    更新日期:2017-03-01 00:00:00

  • Necroptosis in cardiovascular disease - a new therapeutic target.

    abstract::Contrary to the apoptosis-necrosis binary view of cell death, recent experimental evidence demonstrates that several forms of necrosis, represented by necroptosis, are regulated or programmed in nature. Multiple death stimuli known to be associated with cardiovascular disease are capable of causing either apoptosis or...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Gupta K,Phan N,Wang Q,Liu B

    更新日期:2018-05-01 00:00:00

  • A functional role of the C-terminal 42 amino acids of SUR2A and SUR2B in the physiology and pharmacology of cardiovascular ATP-sensitive K(+) channels.

    abstract::The ATP-sensitive K(+) (K(ATP)) channel is composed of four pore-forming Kir6.2 subunits and four sulfonylurea receptors (SUR). Intracellular ATP inhibits K(ATP) channels through Kir6.2. SUR is an ABC protein bearing transmembrane domains and two nucleotide-binding domains (NBD1 and NBD2). SUR increases the open proba...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Yamada M,Kurachi Y

    更新日期:2005-07-01 00:00:00

  • NHE-1 inhibition improves impaired mitochondrial permeability transition and respiratory function during postinfarction remodelling in the rat.

    abstract::The mechanism by which Na+-H+ exchange (NHE) inhibition results in attenuation and reversal of postinfarction remodelling and heart failure remains controversial. In this study, we investigated the possible contribution of mitochondrial involvement by determining the effect of the NHE-1-specific inhibitor EMD-87580 (E...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Javadov S,Huang C,Kirshenbaum L,Karmazyn M

    更新日期:2005-01-01 00:00:00

  • Molecular mechanisms underlying the long-term impact of dietary fat to increase cardiac pyruvate dehydrogenase kinase: regulation by insulin, cyclic AMP and pyruvate.

    abstract::Previous studies have demonstrated that pyruvate dehydrogenase kinase (PDHK) activity in extracts of rat cardiac mitochondria is increased @two-fold by providing a high-fat diet for 28 days. The present study sought to establish the factor(s) that might underlie the response of cardiac PDHK to the provision of a high-...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Sugden MC,Orfali KA,Fryer LG,Holness MJ,Priestman DA

    更新日期:1997-07-01 00:00:00

  • Prevention of abnormal sarcoplasmic reticulum calcium transport and protein expression in post-infarction heart failure using 3, 5-diiodothyropropionic acid (DITPA).

    abstract::Heart failure of diverse causes is associated with abnormalities of sarcoplasmic reticulum (SR) Ca(2+)transport. The purpose of this study was to determine whether the thyroid hormone analogue, 3,5-diiodothyropropionic acid (DITPA), prevents abnormal Ca(2+)transport and expression of SR proteins associated with post-i...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Pennock GD,Spooner PH,Summers CE,Litwin SE

    更新日期:2000-11-01 00:00:00

  • The late Na current as a therapeutic target: where are we?

    abstract::In this article we review the late Na current which functionally can be measured using patch-clamp electrophysiology (INa,late). This current is largely enhanced under pathological myocardial conditions such as ischemia and heart failure. In addition, INa,late can cause systolic and diastolic contractile dysfunction v...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Maier LS,Sossalla S

    更新日期:2013-08-01 00:00:00

  • Alterations in ventricular myocyte contraction caused by C-type natriuretic peptide and nitric oxide in eNOS-/- mice.

    abstract::Lack of endothelial nitric oxide synthase (eNOS) may affect the sensitivity of cyclic GMP signaling through soluble guanylyl cyclase (sGC). We hypothesized that in eNOS knockout (eNOS-/-) mice, stimulation of guanylyl cyclase would have enhanced effects inhibiting cardiac contraction. We measured cell shortening and c...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Su J,Tse J,Scholz PM,Weiss HR

    更新日期:2005-12-01 00:00:00

  • Selective translation of mRNAs in the left ventricular myocardium of the mouse in response to acute pressure overload.

    abstract::During pressure overload hypertrophy, selective changes in cardiac gene expression occur that regulate growth and modify the structural and functional properties of the myocardium. To determine the role of translational mechanisms, a murine model of transverse aortic constriction was used to screen a set of specified ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Spruill LS,Baicu CF,Zile MR,McDermott PJ

    更新日期:2008-01-01 00:00:00

  • Quantifying antiarrhythmic drug blocking during action potentials in guinea-pig papillary muscle.

    abstract::This report examines the blocking effects that several antiarrhythmic drugs have on rapid upstroke velocities of action potentials recorded from guinea-pig papillary muscle. A mathematical analysis is developed, that measures an effective "blocking rate' that acts during the action potential with the purpose being to ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Courtney KR

    更新日期:1983-11-01 00:00:00

  • Gene signatures of postoperative atrial fibrillation in atrial tissue after coronary artery bypass grafting surgery in patients receiving β-blockers.

    abstract::Atrial tissue gene expression profiling may help to determine how differentially expressed genes in the human atrium before cardiopulmonary bypass (CPB) are related to subsequent biologic pathway activation patterns, and whether specific expression profiles are associated with an increased risk for postoperative atria...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Kertai MD,Qi W,Li YJ,Lombard FW,Liu Y,Smith MP,Stafford-Smith M,Newman MF,Milano CA,Mathew JP,Podgoreanu MV,Duke Perioperative Genetics and Safety Outcomes (PEGASUS) Investigative Team.

    更新日期:2016-03-01 00:00:00

  • Targeting the ACE2-Ang-(1-7) pathway in cardiac fibroblasts to treat cardiac remodeling and heart failure.

    abstract::Fibroblasts play a pivotal role in cardiac remodeling and the development of heart failure through the deposition of extra-cellular matrix (ECM) proteins and also by affecting cardiomyocyte growth and function. The renin-angiotensin system (RAS) is a key regulator of the cardiovascular system in health and disease and...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Iwata M,Cowling RT,Yeo SJ,Greenberg B

    更新日期:2011-10-01 00:00:00

  • Extracellular signal-regulated kinase activation during cardiac hypertrophy reduces sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) transcription.

    abstract::Pathologic cardiac hypertrophy can lead to heart failure, but the mechanisms involved are poorly understood. SERCA2 is critical for normal cardiac calcium handling and function and SERCA2 mRNA and protein levels are reduced by cardiac hypertrophy. We hypothesized that extracellular signal-regulated kinase (ERK) 1/2 ac...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Huang H,Joseph LC,Gurin MI,Thorp EB,Morrow JP

    更新日期:2014-10-01 00:00:00

  • Interleukin-1α dependent survival of cardiac fibroblasts is associated with StAR/STARD1 expression and improved cardiac remodeling and function after myocardial infarction.

    abstract:AIMS:One unaddressed aspect of healing after myocardial infarction (MI) is how non-myocyte cells that survived the ischemic injury, keep withstanding additional cellular damage by stress forms typically arising during the post-infarction inflammation. Here we aimed to determine if cell survival is conferred by expressi...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Razin T,Melamed-Book N,Argaman J,Galin I,Lowy Y,Anuka E,Naftali-Shani N,Kandel-Kfir M,Garfinkel BP,Brielle S,Granot Z,Apte RN,Conway SJ,Molkentin JD,Kamari Y,Leor J,Orly J

    更新日期:2020-10-30 00:00:00

  • Effects of hemodynamic variables on myocardial K+ balance during and after shortlasting ischemia.

    abstract::Ischemia-induced myocardial potassium loss and post-ischemic potassium reuptake was quantitated in 8 open chest pigs during control conditions and during hemodynamic alterations which have been shown to increase steady state sarcolemmal potassium fluxes. Myocardial K+ balance was continuously computed before, during a...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Aksnes G,Ellingsen O,Rutlen DL,Ilebekk A

    更新日期:1989-12-01 00:00:00

  • Cardioprotective signaling to mitochondria.

    abstract::Mitochondria are central players in the pathophysiology of ischemia-reperfusion. Activation of plasma membrane G-coupled receptors or the Na,K-ATPase triggers cytosolic signaling pathways that result in cardioprotection. Our working hypothesis is that the occupied receptors migrate to caveolae, where signaling enzymes...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Garlid KD,Costa AD,Quinlan CL,Pierre SV,Dos Santos P

    更新日期:2009-06-01 00:00:00

  • Ischemic but not reperfusion arrhythmias depend upon serum potassium concentration.

    abstract::The effects of variations in serum concentrations of potassium on the occurrence and severity of ischemia- and reperfusion-induced arrhythmias have been studied in conscious rats. Serum potassium concentrations were modified by maintaining rats on diets which varied in potassium concentration, by treatment with hydroc...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Saint KM,Abraham S,MacLeod BA,McGough J,Yoshida N,Walker MJ

    更新日期:1992-07-01 00:00:00

  • microRNA neural networks improve diagnosis of acute coronary syndrome (ACS).

    abstract:BACKGROUND:Cardiac troponins are the preferred biomarkers of acute myocardial infarction. Despite superior sensitivity, serial testing of Troponins to identify patients suffering acute coronary syndromes is still required in many cases to overcome limited specificity. Moreover, unstable angina pectoris relies on report...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Kayvanpour E,Gi WT,Sedaghat-Hamedani F,Lehmann DH,Frese KS,Haas J,Tappu R,Samani OS,Nietsch R,Kahraman M,Fehlmann T,Müller-Hennessen M,Weis T,Giannitsis E,Niederdränk T,Keller A,Katus HA,Meder B

    更新日期:2020-04-17 00:00:00

  • Metabolic inhibition activates a non-selective current through connexin hemichannels in isolated ventricular myocytes.

    abstract::Intracellular Na(+)accumulation and K(+)loss play important roles in the pathogenesis of arrhythmias and injury in the ischemic heart. We investigated the role of metabolically sensitive connexin hemichannels as a potential route for Na(+)influx and K(+)efflux during ischemia, using dye uptake and electrophysiological...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Kondo RP,Wang SY,John SA,Weiss JN,Goldhaber JI

    更新日期:2000-10-01 00:00:00