Growth suppression and immunogenicity enhancement of Hep-2 or primary laryngeal cancer cells by adenovirus-mediated co-transfer of human wild-type p53, granulocyte-macrophage colony-stimulating factor and B7-1 genes.


:Co-transfer of immunomodulatory and antiproliferative genes may be the basis for new strategies to potentiate tumor regression. In this study, we evaluated the in vitro effect of the introduction of human wild-type p53, granulocyte-macrophage colony-stimulating factor (GM-CSF), and B7-1 genes via recombinant adenovirus on the growth and immunogenicity of Hep-2 or primary laryngeal cancer cells. By the introduction of wild-type p53 gene, the growth of Hep-2 cells was inhibited via enhanced apoptosis. By the introduction of GM-CSF and B7-1 genes, the immunogenicity of cancer cells was enhanced. Significant proliferation of tumor infiltrating lymphocytes (TILs) and tumor-specific cytotoxicity of cytotoxic T lymphocytes (CTLs) were induced in vitro. Furthermore, the combinative effect of GM-CSF and B7-1 was even more evident than that of any one of them singly. These results suggest that the co-transfer of human wild-type p53, GM-CSF and B7-1 genes into tumor cells via recombinant adenovirus may be further developed into a potential combination gene therapy strategy for cancer.


Cancer Lett


Cancer letters


Qiu ZH,Wu CT,Lao MF,Pan LZ,Li YM





Has Abstract


2002-08-28 00:00:00














  • Study of postnatal effects of chemopreventive agents on offspring of ethylnitrosourea-induced transplacental carcinogenesis in rats. I. Influence of retinol acetate, alpha-tocopherol acetate, thiamine chloride, sodium selenite, and alpha-difluoromethylorn

    abstract::We studied the influence of the vitamins retinol acetate, alpha-tocopherol acetate and thiamine chloride; the antioxidant sodium selenite and an inhibitor of polyamine biosynthesis, alpha-difluoromethylornithine, on the offspring of transplacental carcinogenesis by ethylnitrosourea in rats. Ethylnitrosourea was given ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Alexandrov VA,Bespalov VG,Boone CW,Kelloff GJ,Malone WF

    更新日期:1991-11-01 00:00:00

  • Complexity, chaos and human physiology: the justification for non-linear neural computational analysis.

    abstract::Background is presented to suggest that a great many biologic processes are chaotic. It is well known that chaotic processes can be accurately characterized by non-linear technologies. Evidence is presented that an artificial neural network, which is a known method for the application of non-linear statistics, is able...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Baxt WG

    更新日期:1994-03-15 00:00:00

  • Histological comparison of the B16 melanoma and its F1 variant.

    abstract::The B16 malignant melanoma and its F1 variant line were described and compared in syngeneic C57BL/6 mice at short and long term periods after transplantation. No observable differences in gross or histological features were noted. In both lines, liver and lung metastasis was confirmed and kidney colonization proposed....

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Wosko TJ,Ferrara DT,Sartori LS

    更新日期:1984-08-01 00:00:00

  • Helicobacter pylori upregulates Nanog and Oct4 via Wnt/β-catenin signaling pathway to promote cancer stem cell-like properties in human gastric cancer.

    abstract::Helicobacter pylori (H. pylori) infection is considered a major risk factor for gastric cancer. CagA behaves as a major bacterial oncoprotein playing a key role in H. pylori-induced tumorigenesis. Cancer stem cells (CSCs) are believed to possess the ability to initiate tumorigenesis and promote progression. Although s...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Yong X,Tang B,Xiao YF,Xie R,Qin Y,Luo G,Hu CJ,Dong H,Yang SM

    更新日期:2016-05-01 00:00:00

  • Translocations involving chromosome 1 in hormone-independent GR mouse mammary tumors.

    abstract::Large marker chromosomes were found in 2 hormone-independent (HI) GR mouse mammary tumors, i.e. a tumor (TSl 75) that was already HI at the first transplantation, and a tumor (TSl 66) that became HI at the 19th passage. These marker chromosomes appear to be translocations to distal and proximal ends of chromosome 1, r...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Sluyser M,Evers P,Hilgers J

    更新日期:1985-06-01 00:00:00

  • Dacomitinib antagonizes multidrug resistance (MDR) in cancer cells by inhibiting the efflux activity of ABCB1 and ABCG2 transporters.

    abstract::The development of multidrug resistance (MDR) to chemotherapy remains a major challenge in the treatment of cancer. Numerous mechanisms have been recognized that cause MDR, but one of the most important mechanisms is overexpression of adenosine triphosphate (ATP)-binding cassette (ABC) transporters, through which the ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Fan YF,Zhang W,Zeng L,Lei ZN,Cai CY,Gupta P,Yang DH,Cui Q,Qin ZD,Chen ZS,Trombetta LD

    更新日期:2018-05-01 00:00:00

  • Tumor-initiating activity of the (+)-(S,S)- and (-)-(R,R)- enantiomers of trans-11,12-dihydroxy-11,12-dihydrodibenzo[a,l]pyrene in mouse skin.

    abstract::A single administration of enantiomerically pure 11,12-dihydrodiols of dibenzo[a,l]pyrene (DB[a,l]P) on the back of NMRI mice and subsequent chronic treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA) (initiation/promotion assay) revealed strikingly different carcinogenic activities of both enantiomers. Tumor-in...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Luch A,Friesel H,Seidel A,Platt KL

    更新日期:1999-03-01 00:00:00

  • Histone deacetylase inhibitors prevent activation of tumour-reactive NK cells and T cells but do not interfere with their cytolytic effector functions.

    abstract::Histone deacetylase inhibitors (HDIs) exert direct tumour-toxic activity and sensitise tumour cells for other therapeutic regimens as well as the cytotoxic effects of activated immune cells. However, the HDI suberoylanilide hydroxamic acid (SAHA; vorinostat) interfered with the IL-2 activation of human NK cells and th...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Schmudde M,Friebe E,Sonnemann J,Beck JF,Bröker BM

    更新日期:2010-09-28 00:00:00

  • Distinct mechanisms for opposite functions of homeoproteins Cdx2 and HoxB7 in double-strand break DNA repair in colon cancer cells.

    abstract::Homeobox genes, involved in embryonic development and tissues homeostasis in adults, are often deregulated in cancer, but their relevance in pathology is far from being fully elucidated. In colon cancers, we report that the homeoproteins HoxB7 and Cdx2 exhibit different heterogeneous patterns, Cdx2 being localized in ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Soret C,Martin E,Duluc I,Dantzer F,Vanier M,Gross I,Freund JN,Domon-Dell C

    更新日期:2016-05-01 00:00:00

  • Enhanced IkappaB kinase activity is responsible for the augmented activity of NF-kappaB in human head and neck carcinoma cells.

    abstract::The nuclear transcription factor kappaB (NF-kappaB) plays an important role in the development and progression of cancers. However, the mechanism by which cancer cells in the head and neck region acquire high NF-kappaB activity has not yet been clarified. In this study, we examined the NF-kappaB binding activity and t...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Tamatani T,Azuma M,Aota K,Yamashita T,Bando T,Sato M

    更新日期:2001-10-10 00:00:00

  • Effect of human procathepsin D on proliferation of human cell lines.

    abstract::We have used human procathepsin D isolated from supernatant of human breast cancer cell line ZR-75-1 to test its mitogenic activity for a broad spectrum of human-derived cell lines. These cell lines included: breast cancer cell lines ZR-75-1, MDA-MB-436, MBA-MD-483 and MDA-MB-231, B lymphoblastoid cell line Raji, the ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Vĕtvicka V,Vĕktvicková J,Fusek M

    更新日期:1994-05-16 00:00:00

  • Nutlin-3 inhibits epithelial-mesenchymal transition by interfering with canonical transforming growth factor-β1-Smad-Snail/Slug axis.

    abstract::Enormous efforts have been made to explore small molecules that interfere with the TGF-β signaling pathway, so as to inhibit EMT and the cancer metastasis, but few agents have been identified. In this study, we demonstrated that Nutlin-3 could abolish the down-regulation of E-cadherin induced by TGF-β1 in p53-deficien...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Wu Y,Fu Y,Zheng L,Lin G,Ma J,Lou J,Zhu H,He Q,Yang B

    更新日期:2014-01-01 00:00:00

  • Systemic alterations in ornithine decarboxylase activity caused by colon cancer in mice.

    abstract::Tumors are known to cause profound changes in host biology, but the mechanisms responsible for these changes remain unclear. Ornithine decarboxylase (ODC) is a rate-limiting enzyme that catalyzes the biosynthesis of polyamines. The purpose of this study was to examine the effects of MC-26 tumor burden on ODC activity ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Saydjari R,Beauchamp RD,Townsend CM Jr,Thompson JC

    更新日期:1991-06-14 00:00:00

  • Glioma initiating cells contribute to malignant transformation of host glial cells during tumor tissue remodeling via PDGF signaling.

    abstract:INTRODUCTION:Glioma initiating cells (GICs) play important roles in tumor initiation and progression. However, interactions between tumor cells and host cells of local tumor microenvironment are kept largely unknown. Besides GICs and their progeny cells, whether adjacent normal glial cells contribute to tumorigenesis d...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Chen Y,Wang Z,Dai X,Fei X,Shen Y,Zhang M,Wang A,Li X,Wang Z,Huang Q,Dong J

    更新日期:2015-09-01 00:00:00

  • Heat shock (hsp70) and resistance proteins in non-small cell lung carcinomas.

    abstract::The aim of the study was to prove whether or not an association exists between the heat shock protein 70 (hsp70) and drug resistance. Tumor samples of 90 patients with previously untreated non-small lung carcinomas were investigated immunohistochemically for expression of resistance related proteins. Additionally, res...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Volm M,Koomägi R,Mattern J,Stammler G

    更新日期:1995-08-16 00:00:00

  • Clastogenic and cytotoxic effects of spermine oxidation products in mouse lymphoma L5178Y cells.

    abstract::Treatment of mouse lymphoma L5178Y cells with spermine in Fischer's medium containing bovine serum lead to formation of chromatid aberrations. The cause of this effect seems to be that bovine serum contains spermine oxidase which catalyzes oxidation of spermine to unstable aldehyde derivatives (oxidized spermine) and ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Bouzyk E,Rosiek O

    更新日期:1988-02-01 00:00:00

  • Expression analysis of endogenous retroviral elements belonging to the HERV-F family from human tissues and cancer cells.

    abstract::Based on the primer binding site for Phe-tRNA, human endogenous retroviruses (HERVs) have been classified as HERV-F family members, HERV-F, HERV-FXA, HERV-Fb, and HERV-Fc. The HERV-F family was expressed in human placenta preferentially. We investigated pol fragments of HERV-F family in various human tissues and cance...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Yi JM,Kim HS

    更新日期:2004-07-28 00:00:00

  • Variability of origin for the neocentromeric sequences in analphoid supernumerary marker chromosomes of well-differentiated liposarcomas.

    abstract::Well-differentiated liposarcomas (WDLPS) and dedifferentiated liposarcomas are cytogenetically characterized by the presence of supernumerary ring or giant chromosomes containing amplified material from the 12q14-15 region. These chromosomes contain neocentromeres, which are able to bind the kinetochore proteins and t...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Italiano A,Maire G,Sirvent N,Nuin PA,Keslair F,Foa C,Louis C,Aurias A,Pedeutour F

    更新日期:2009-01-18 00:00:00

  • Pre-malignant transformation by senescence evasion is prevented by the PERK and ATF6alpha branches of the Unfolded Protein Response.

    abstract::The incidence of carcinomas highly increases with age. However, the initial steps of the age-related molecular carcinogenic processes remain poorly characterized. We previously showed that normal human epidermal keratinocytes spontaneously and systematically escape from senescence to give rise to preneoplastic emergin...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Drullion C,Marot G,Martin N,Desle J,Saas L,Salazar-Cardozo C,Bouali F,Pourtier A,Abbadie C,Pluquet O

    更新日期:2018-12-01 00:00:00

  • MELK is an oncogenic kinase essential for early hepatocellular carcinoma recurrence.

    abstract::Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related deaths worldwide. Many kinases have been found to be intimately involved in oncogenesis and the deregulation of kinase function has emerged as a major mechanism by which cancer cells evade normal physiological constraints on growth and surviv...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Xia H,Kong SN,Chen J,Shi M,Sekar K,Seshachalam VP,Rajasekaran M,Goh BKP,Ooi LL,Hui KM

    更新日期:2016-12-01 00:00:00

  • Potential implications of mesenchymal stem cells in cancer therapy.

    abstract::Mesenchymal stem cells (MSCs) are the first type of stem cells to be utilized in clinical regenerative medicine, mainly owing to their capacity for multipotent differentiation and the feasibility of autologous transplantation. More recently, the specific tumor-oriented migration and incorporation of MSCs have been dem...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Dai LJ,Moniri MR,Zeng ZR,Zhou JX,Rayat J,Warnock GL

    更新日期:2011-06-01 00:00:00

  • Use of a tetrazolium based colorimetric assay in assessing photoradiation therapy in vitro.

    abstract::The suitability of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] assay to the determination of cell viability following photoradiation therapy (PRT) of human breast and melanoma cell lines has been examined. Results have been shown to correlate with those obtained using a clonogen...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: McHale AP,McHale L

    更新日期:1988-08-30 00:00:00

  • Selective hyperexpression of c-jun oncoprotein by glass fiber- and silica-transformed BALB/c-3T3 cells.

    abstract::Mining and mineral processing are important industries in the United States. A large number of workers are potentially exposed to silica during mining and to glass fibers during manufacturing. There is a concern regarding lung cancer risk among workers exposed to silica and glass fibers. Our previous studies showed th...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Gao H,Brick J,Ong S,Miller M,Whong WZ,Ong T

    更新日期:1997-01-15 00:00:00

  • Induction of apoptosis in human lymphocytes treated with Viscum album L. is mediated by the mistletoe lectins.

    abstract::Viscum album L. (VAL) is a phytopreparation used in adjuvant cancer therapy with both immunostimulatory and DNA stabilizing properties at low drug concentrations and cytostatic/cytotoxic properties at higher concentrations. The present work examines the cytotoxic effects of VAL extracts produced from mistletoes grown ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Büssing A,Suzart K,Bergmann J,Pfüller U,Schietzel M,Schweizer K

    更新日期:1996-01-19 00:00:00

  • The predictive power of CD3+ T cell infiltration of oral squamous cell tumors is limited to non-diabetic patients.

    abstract::Diabetes mellitus type II (DM) and immune cell infiltration determine patient outcome in many tumor entities. Here we studied a possible link between the metabolic and immune cell status of OSCC patients. Glucose transporter (GLUT) 1 mRNA expression was elevated in all tumor samples, whereas other glycolytic markers s...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Spanier G,Ugele I,Nieberle F,Symeou L,Schmidhofer S,Brand A,Meier J,Spoerl S,Krupar R,Rümmele P,Siska P,Renner K,Peter K,Gerken M,Beckhove P,Reichert TE,Kreutz M,Singer K

    更新日期:2021-02-28 00:00:00

  • 5-(3-Hydroxymethyl-3-methyl-1-triazeno imidazole-4-carboxamide is a metabolite of 5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide (DIC, DTIC NSC-45388).

    abstract::The cancer chemotherapeutic drug, 5-(3,3-dimethyl-1-triazeno) imidazole-4-carboxamide (DIC, DTIC, NSC-45388), is metabolised in rats to a structurally related product which was detected by thin-layer chromatography. The novel metabolite has a lower mobility and a colour reaction that is indistinguishable from the pare...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Kolar GF,Maurer M,Wildschütte M

    更新日期:1980-09-01 00:00:00

  • Cancer heterogeneity and "the struggle for existence": diagnostic and analytical challenges.

    abstract::The notions of inter- and intra-tumour heterogeneity (ITH) have been recognised for many years but recent advances in sequencing technology are allowing the true extent of both forms of heterogeneity to be revealed in detail for the first time. In this review we examine the current evidence for ITH, the possibility of...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Horswell S,Matthews N,Swanton C

    更新日期:2013-11-01 00:00:00

  • Nemo-like kinase (NLK) primes colorectal cancer progression by releasing the E2F1 complex from HDAC1.

    abstract::Control of E2F1 activity is restricted via its interactions with RB1 and HDAC1. However, the detailed regulatory mechanisms underlying the E2F1/HDAC1 complex remain elusive. Here, we report that Nemo-like kinase (NLK) boosts cell cycle progression, which facilitates tumor development by releasing the E2F1 protein from...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Li SZ,Zeng F,Li J,Shu QP,Zhang HH,Xu J,Ren JW,Zhang XD,Song XM,Du RL

    更新日期:2018-09-01 00:00:00

  • Reduction in NaCl-enhanced gastric carcinogenesis in rats fed a high-protein diet.

    abstract::The effect of a purified, high protein diet on enhanced gastric carcinogenesis induced by oral administration of NaCl was investigated in Wistar rats. Rats were fed on a purified diet with an equalized caloric content, containing 8% NaCl and 25% casein (normal protein diet), or 50% casein (high protein diet) after ora...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Tatsuta M,Iishi H,Baba M,Uehara H,Nakaizumi A,Iseki K

    更新日期:1997-06-24 00:00:00

  • The inhibitory action of BOF-A2, a 5-fluorouracil derivative, on squamous cell carcinoma.

    abstract::Inactivation of antineoplastics is a serious problem in cancer therapy, and prevention of the inactivation is a surpassing strategy for enhancement of their therapeutic effects. BOF-A2, which contains both EM-FU, a masked form of 5-fluorouracil (5-FU), and CNDP, an inhibitor of 5-FU degradation, was developed with thi...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Yoneda K,Yamamoto T,Ueta E,Osaki T

    更新日期:1999-03-22 00:00:00