Rho/Rho-kinase is involved in the synthesis of tissue factor in human monocytes.


:Monocytes and macrophages synthesize tissue factor (TF) which plays a role in thrombogenicity in coronary artery disease. This study was conducted to investigate the effect of Rho/Rho-kinase inhibition on the synthesis of TF in cultured human monocytes. 3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (statins), C3 exoenzyme and Rho-kinase inhibitors were added to isolated peripheral blood monocytes and the synthesis of TF was assessed by reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry. Rho activity was determined by measuring the GTP-bound form of Rho A. Cerivastatin and pravastatin reduced the levels of TF antigen and mRNA. The suppressive effect of statins on TF synthesis was reversed by geranylgeranylpyrophosphate (GGPP) and the restoring effect of GGPP was eliminated by C3 exoenzyme and Y-27632. Pravastatin decreased the activity of Rho A, suggesting that the suppression of TF synthesis by statins is mediated via inhibition of the geranylgeranylation of Rho. Moreover, inhibition of Rho and Rho-kinase downregulated the synthesis of TF. Our results suggest that Rho/Rho-kinase signaling is involved in the synthesis of TF in human monocytes and that inhibition of Rho/Rho-kinase may be useful for treating thrombogenicity in coronary artery disease.






Nagata K,Ishibashi T,Sakamoto T,Ohkawara H,Shindo J,Yokoyama K,Sugimoto K,Sakurada S,Takuwa Y,Nakamura S,Teramoto T,Maruyama Y





Has Abstract


2002-07-01 00:00:00














  • The P2Y2 nucleotide receptor is an inhibitor of vascular calcification.

    abstract:BACKGROUND AND AIMS:Mutations in the 5'-nucleotidase ecto (NT5E) gene that encodes CD73, a nucleotidase that converts AMP to adenosine, are linked to arterial calcification. However, the role of purinergic receptor signaling in the pathology of intimal calcification is not well understood. In this study, we examined wh...


    pub_type: 杂志文章


    authors: Qian S,Regan JN,Shelton MT,Hoggatt A,Mohammad KS,Herring PB,Seye CI

    更新日期:2017-02-01 00:00:00

  • Arachidonic acid of platelet phospholipids is decreased after extracorporeal removal of plasma low density lipoproteins in patients with familial hypercholesterolemia.

    abstract::Platelet phospholipid composition was analyzed before and after extracorporeal removal of low density lipoproteins (LDL) by LDL apheresis in six patients with familial hypercholesterolemia. Elevated levels of total plasma cholesterol and the portion of plasma cholesterol carried by LDL were reduced by 56 and 66% after...


    pub_type: 杂志文章


    authors: Bräutigam R,Bräutigam C,Lorenz R,Richter WO,Engelmann B

    更新日期:1997-05-01 00:00:00

  • Adipose tissue triglyceride fatty acids and atherosclerosis in Alaska Natives and non-Natives.

    abstract::Essential polyunsaturated fatty acids (PUFA) of the omega-3 family are believed to protect against cardiovascular disease. A rich source of omega-3 PUFA is found in fish and marine mammals (seal, walrus, whale), which are a large part of the traditional diet of Alaska Natives (Eskimo, American Indians, Aleuts), a grou...


    pub_type: 杂志文章


    authors: McLaughlin J,Middaugh J,Boudreau D,Malcom G,Parry S,Tracy R,Newman W

    更新日期:2005-08-01 00:00:00

  • The NLRP3 inflammasome and the emerging role of colchicine to inhibit atherosclerosis-associated inflammation.

    abstract::Atherosclerosis is considered a chronic inflammatory disease of the arterial wall. Recently, compelling evidence has arisen for the role of monocytes and neutrophils and a particular protein complex that resides within these cells - the NLRP3 inflammasome - in atherosclerosis-associated inflammation. It is now also kn...


    pub_type: 杂志文章,评审


    authors: Martínez GJ,Celermajer DS,Patel S

    更新日期:2018-02-01 00:00:00

  • Comparative effects of polyunsaturated- to saturated fatty acid ratio versus polyunsaturated- and monounsaturated fatty acids to saturated fatty acid ratio on lipid metabolism in rats.

    abstract::This study examined the effects of various polyunsaturated fatty acid (PUFA, P)/saturated fatty acid (SFA, S) ratio versus PUFA + monounsaturated fatty acid (MUFA, M)/SFA ratio on lipid metabolism. The P/S ratio of dietary fat was fixed at a certain level (0.5, 1, 2, or 4, respectively) for each of four pairs of rat g...


    pub_type: 杂志文章


    authors: Chang NW,Huang PC

    更新日期:1999-01-01 00:00:00

  • Endothelial progenitor cells as therapeutic agents in the microcirculation: an update.

    abstract::This review evaluates novel beneficial effects of circulating endothelial progenitor cells (EPCs) as shown by several preclinical studies and clinical trials carried out to test the safety and feasibility of using EPCs. There are 31 registered clinical trials (and many others still ongoing) and 19 published studies. E...


    pub_type: 杂志文章,评审


    authors: Napoli C,Hayashi T,Cacciatore F,Casamassimi A,Casini C,Al-Omran M,Ignarro LJ

    更新日期:2011-03-01 00:00:00

  • Interrelationships between plasma levels of plasminogen activator inhibitor, tissue plasminogen activator, lipoprotein (a), and established cardiovascular risk factors in a north Swedish population.

    abstract::Serum lipids, lipoprotein (a), plasminogen activator inhibitor and tissue plasminogen activator levels were measured in 260 subjects, constituting a cross-section sample of 30-60-year-old men and women. For Lp(a), there were positive correlations with age and cholesterol, but not with any of other measured parameters....


    pub_type: 杂志文章


    authors: Sundell IB,Nilsson TK,Hallmans G,Hellsten G,Dahlén GH

    更新日期:1989-11-01 00:00:00

  • 18Fluoride-based molecular imaging of coronary atherosclerosis in HIV infected patients.

    abstract:BACKGROUND AND AIMS:Molecular imaging with 18Fluorodeoxyglucose (FDG) and 18F-sodium-fluoride (NaF) captures arterial inflammation and micro-calcification and can reveal potentially unstable atherosclerotic plaques. METHODS:We performed FDG and NaF PET/CT imaging in two clinically similar cohorts of patients living wi...


    pub_type: 杂志文章


    authors: Guaraldi G,Milic J,Prandini N,Ligabue G,Esposito F,Ciusa G,Malagoli A,Scaglioni R,Besutti G,Beghetto B,Nardini G,Roncaglia E,Mussini C,Raggi P

    更新日期:2020-03-01 00:00:00

  • Genetics of cardiovascular disease: Importance of sex and ethnicity.

    abstract::Sex differences in incidence and prevalence of and morbidity and mortality from cardiovascular disease are well documented. However, many studies examining the genetic basis for cardiovascular disease fail to consider sex as a variable in the study design, in part, because there is an inherent difficulty in studying t...


    pub_type: 杂志文章,评审


    authors: Winham SJ,de Andrade M,Miller VM

    更新日期:2015-07-01 00:00:00

  • CETP inhibitors downregulate hepatic LDL receptor and PCSK9 expression in vitro and in vivo through a SREBP2 dependent mechanism.

    abstract:BACKGROUND:CETP inhibitors block the transfer of cholesteryl ester from HDL-C to VLDL-C and LDL-C, thereby raising HDL-C and lowering LDL-C. In this study, we explored the effect of CETP inhibitors on hepatic LDL receptor (LDLR) and PCSK9 expression and further elucidated the underlying regulatory mechanism. RESULTS:W...


    pub_type: 杂志文章


    authors: Dong B,Singh AB,Fung C,Kan K,Liu J

    更新日期:2014-08-01 00:00:00

  • Array-based resequencing for mutations causing familial hypercholesterolemia.

    abstract:BACKGROUND:Familial hypercholesterolemia (FH) is a heterogeneous autosomal dominant disease with a prevalence of 1 in 500. To date, over 1200 unique pathogenic mutations have been identified in at least 3 genes. The large allelic and genetic heterogeneity of FH requires high-throughput, rapid, and affordable mutation d...


    pub_type: 杂志文章


    authors: Chiou KR,Charng MJ,Chang HM

    更新日期:2011-06-01 00:00:00

  • Carotid and femoral atherosclerotic plaques show different morphology.

    abstract:OBJECTIVE:Results of endovascular repair vary according to the arterial bed. We hypothesized that these differences may be related to the plaque features. To explore this hypothesis, we designed a prospective study that compared carotid and femoral atheroma. METHODS AND RESULTS:Patients that underwent femoral or carot...


    pub_type: 杂志文章


    authors: Herisson F,Heymann MF,Chétiveaux M,Charrier C,Battaglia S,Pilet P,Rouillon T,Krempf M,Lemarchand P,Heymann D,Gouëffic Y

    更新日期:2011-06-01 00:00:00

  • Plasma levels of VLDL- + LDL-cholesterol, HDL-cholesterol, triglycerides and apoproteins B and A-I in a healthy population--influence of several risk factors.

    abstract::Plasma apo B and apo A-I were determined in 477 subjects (206 males and 271 females) by laser immuno-nephelometry. Measurements of VLDL- + LDL-cholesterol, HDL-cholesterol and triglycerides were done simultaneously. VLDL- + LDL-cholesterol and apo B values were similar in males and females and increased with age. HDL-...


    pub_type: 杂志文章


    authors: Dedonder-Decoopman E,Fievet-Desreumaux C,Campos E,Moulin S,Dewailly P,Sezille G,Jaillard J

    更新日期:1980-12-01 00:00:00

  • Increased lipid peroxidation as a mechanism of methionine-induced atherosclerosis in rabbits.

    abstract::Methionine is converted by the transmethylation/transsulfuration pathway to homocysteine which may exert atherogenic effects by several mechanisms, including lipid peroxidation. Therefore, the excessive dietary methionine may induce the development of atherosclerosis. To test this hypothesis, plasma and aortic thiobar...


    pub_type: 杂志文章


    authors: Toborek M,Kopieczna-Grzebieniak E,Drózdz M,Wieczorek M

    更新日期:1995-06-01 00:00:00

  • The SH2B1 obesity locus is associated with myocardial infarction in diabetic patients and with NO synthase activity in endothelial cells.

    abstract:OBJECTIVE:Obesity and cardiovascular disease recognize a common metabolic soil and may therefore share part of their genetic background. Genome-wide association studies have identified variability at the SH2B1 locus as a predictor of obesity. We investigated whether SNP rs4788102, which captures the entire SH2B1 variab...


    pub_type: 杂志文章,多中心研究


    authors: Prudente S,Morini E,Larmon J,Andreozzi F,Di Pietro N,Nigro A,Gervino EV,Mannino GC,Bacci S,Hauser TH,Bellacchio E,Formoso G,Pellegrini F,Proto V,Menzaghi C,Frittitta L,Pandolfi A,Sesti G,Doria A,Trischitta V

    更新日期:2011-12-01 00:00:00

  • Galectin-3 binding protein, coronary artery disease and cardiovascular mortality: Insights from the LURIC study.

    abstract:BACKGROUND AND AIMS:Galectin-3 binding protein (Gal-3BP) has been associated with inflammation and cancer, however, its role in coronary artery disease (CAD) and cardiovascular outcome remains unclear. METHODS:Gal-3BP plasma levels were measured by ELISA in 2922 individuals from the LURIC study (62.7 ± 10.6 years, 62....


    pub_type: 杂志文章


    authors: Gleissner CA,Erbel C,Linden F,Domschke G,Akhavanpoor M,Helmes CM,Doesch AO,Kleber ME,Katus HA,Maerz W

    更新日期:2017-05-01 00:00:00

  • Impact of CD14++CD16+ monocytes on coronary plaque vulnerability assessed by optical coherence tomography in coronary artery disease patients.

    abstract:BACKGROUND AND AIMS:This study examined the impact of CD14++CD16+ monocytes on coronary plaque vulnerability, as assessed by optical coherence tomography (OCT), and investigated their association with daily glucose fluctuation. Although increased CD14++CD16+ monocyte levels have been reported to increase cardiovascular...


    pub_type: 杂志文章


    authors: Yamamoto H,Yoshida N,Shinke T,Otake H,Kuroda M,Sakaguchi K,Hirota Y,Toba T,Takahashi H,Terashita D,Uzu K,Tahara N,Shinkura Y,Kuroda K,Nagasawa Y,Nagano Y,Tsukiyama Y,Yanaka KI,Emoto T,Sasaki N,Yamashita T,Ogawa

    更新日期:2018-02-01 00:00:00

  • Detection and localization of tumor necrosis factor-alpha in WHHL rabbit arteries.

    abstract::Tumor necrosis factor-alpha (TNF-alpha) is known to be a secretory product of activated macrophages. TNF-alpha activates endothelial cells, stimulates angiogenesis and induces proliferation of smooth muscle cells (SMCs). Therefore, TNF-alpha has been suggested to be actively involved in the inflammatory events associa...


    pub_type: 杂志文章


    authors: Lei X,Buja LM

    更新日期:1996-08-23 00:00:00

  • Sleep duration is significantly associated with carotid artery atherosclerosis incidence in a Japanese population.

    abstract:BACKGROUND:Previous studies have indicated that sleep duration is associated with total mortality in a U-shaped fashion. The purpose of the current study was to examine the relationship between self-reported sleep duration and carotid artery atherosclerosis in a Japanese population. METHODS:In 2009-2010, a total of 24...


    pub_type: 杂志文章


    authors: Abe T,Aoki T,Yata S,Okada M

    更新日期:2011-08-01 00:00:00

  • Effects of ezetimibe added to statin therapy on markers of cholesterol absorption and synthesis and LDL-C lowering in hyperlipidemic patients.

    abstract:OBJECTIVE:Statins inhibit cholesterol synthesis but can upregulate cholesterol absorption, with higher doses producing larger effects. Ezetimibe inhibits cholesterol absorption but also upregulates synthesis. We tested whether ezetimibe added to on-going statin therapy would be most effective in lowering LDL-cholestero...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Thongtang N,Lin J,Schaefer EJ,Lowe RS,Tomassini JE,Shah AK,Tershakovec AM

    更新日期:2012-12-01 00:00:00

  • Long non-coding RNAs in the atherosclerotic plaque.

    abstract:BACKGROUND AND AIMS:Genetic and environmental factors are important components of the development of atherosclerosis. Long non-coding RNA (lncRNAs) have emerged as regulators of multiple pathophysiological pathways in the cardiovascular system. Here, we investigated potential associations between lncRNAs and atheroscle...


    pub_type: 杂志文章


    authors: Arslan S,Berkan Ö,Lalem T,Özbilüm N,Göksel S,Korkmaz Ö,Çetin N,Devaux Y,Cardiolinc™ network.

    更新日期:2017-11-01 00:00:00

  • Plaque microstructures in patients with coronary artery disease who achieved very low low-density lipoprotein cholesterol levels.

    abstract:OBJECTIVE:Lowering low-density lipoprotein cholesterol (LDL-C) with statins reduces cardiovascular events and slows plaque progression. While this therapeutic approach has been reported to favorably modify plaque composition, this is not well characterized in humans. Also, the benefit of achieving LDL-C levels below cu...


    pub_type: 杂志文章


    authors: Kataoka Y,Hammadah M,Puri R,Duggal B,Uno K,Kapadia SR,Murat Tuzcu E,Nissen SE,Nicholls SJ

    更新日期:2015-10-01 00:00:00

  • Identification and characterization of new gain-of-function mutations in the PCSK9 gene responsible for autosomal dominant hypercholesterolemia.

    abstract:BACKGROUND:The identification of mutations in PCSK9 (proprotein convertase subtilisin kexin9) in autosomal dominant hypercholesterolemia (ADH), has revealed the existence of a new player in cholesterol homeostasis. PCSK9 has been shown to enhance the degradation of the LDL receptor (LDLR) at the cell surface. Gain-of-f...


    pub_type: 杂志文章


    authors: Abifadel M,Guerin M,Benjannet S,Rabès JP,Le Goff W,Julia Z,Hamelin J,Carreau V,Varret M,Bruckert E,Tosolini L,Meilhac O,Couvert P,Bonnefont-Rousselot D,Chapman J,Carrié A,Michel JB,Prat A,Seidah NG,Boileau C

    更新日期:2012-08-01 00:00:00

  • Impact of evolocumab treatment on low-density lipoprotein cholesterol levels in heterozygous familial hypercholesterolemic patients withdrawing from regular apheresis.

    abstract:BACKGROUND AND AIMS:Low-density lipoprotein (LDL) apheresis has been used to treat refractory hyperlipidemia such as familial hypercholesterolemia (FH). Evolocumab, a proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor used in clinical settings, can reduce LDL cholesterol (LDL-C) levels by >70%. Therefore, ...


    pub_type: 杂志文章,多中心研究


    authors: Kawashiri MA,Nohara A,Higashikata T,Tada H,Nakanishi C,Okada H,Konno T,Sakata K,Hayashi K,Inazu A,Mabuchi H,Yamagishi M

    更新日期:2017-10-01 00:00:00

  • Alterations in composition and concentration of lipoproteins and elevated cholesteryl ester transfer in non-insulin-dependent diabetes mellitus (NIDDM).

    abstract::Cholesteryl esters (CE) exchange between lipoproteins through the action of cholesteryl ester transfer protein (CETP). Situations at high risk for atherosclerosis are often accompanied by an accelerated net mass CE transfer (CET) from high density lipoproteins (HDL) to very low (VLDL) and low density lipoproteins (LDL...


    pub_type: 杂志文章


    authors: Elchebly M,Porokhov B,Pulcini T,Berthezène F,Ponsin G

    更新日期:1996-06-01 00:00:00

  • Lysosomal acid lipase deficiency--an under-recognized cause of dyslipidaemia and liver dysfunction.

    abstract::Lysosomal acid lipase deficiency (LAL-D) is a rare autosomal recessive lysosomal storage disease caused by deleterious mutations in the LIPA gene. The age at onset and rate of progression vary greatly and this may relate to the nature of the underlying mutations. Patients presenting in infancy have the most rapidly pr...


    pub_type: 杂志文章,评审


    authors: Reiner Ž,Guardamagna O,Nair D,Soran H,Hovingh K,Bertolini S,Jones S,Ćorić M,Calandra S,Hamilton J,Eagleton T,Ros E

    更新日期:2014-07-01 00:00:00

  • Cross-sectional and longitudinal associations between serum bilirubin and dyslipidemia in a health screening population.

    abstract:OBJECTIVE:To investigate cross-sectional and longitudinal associations between serum total bilirubin (TB) and dyslipidemia. METHODS:Odds ratios (ORs) of prevalent dyslipidemia for TB were calculated in 2113 men and 1265 women. Correlation coefficients between baseline TB as well as the change in TB over 5 years and ba...


    pub_type: 杂志文章


    authors: Oda E

    更新日期:2015-03-01 00:00:00

  • LDL concentration is correlated with the removal from the plasma of a chylomicron-like emulsion in subjects with coronary artery disease.

    abstract::In animal model studies, the uptake of chylomicron remnants after entering in the space of Disse occurs mainly by low-density lipoprotein (LDL) receptor and LDL receptor-related protein (LRP). In subjects, the relative importance of each one of these receptors for the clearance of chylomicron remnants is not fully und...


    pub_type: 杂志文章


    authors: Sposito AC,Santos RD,Hueb W,Ventura LI,Vinagre CC,Ramires JA,Maranhão RC

    更新日期:2002-04-01 00:00:00

  • 7-ketocholesteryl-9-carboxynonanoate enhances ATP binding cassette transporter A1 expression mediated by PPARγ in THP-1 macrophages.

    abstract:BACKGROUND:ATP binding cassette transporter A1 (ABCA1) is a member of the ATP-binding cassette transporter family. It plays an essential role in mediating the efflux of excess cholesterol. It is known that peroxisome proliferator-activated receptor gamma (PPARγ) promoted ABCA1 expression. We previously found 7-ketochol...


    pub_type: 杂志文章


    authors: Chi Y,Wang L,Liu Y,Ma Y,Wang R,Han X,Qiao H,Lin J,Matsuura E,Liu S,Liu Q

    更新日期:2014-06-01 00:00:00

  • Prevention of focal intimal hyperplasia in rat vein grafts by using a tissue engineering approach.

    abstract::The present study focused on the role of blood flow in the formation of focal intimal hyperplasia in vein grafts, as well as the development of an engineering approach that can be used to eliminate disturbed blood flow and prevent blood flow-related focal intimal hyperplasia. A rat vein graft model was constructed by ...


    pub_type: 杂志文章


    authors: Liu SQ

    更新日期:1998-10-01 00:00:00