Catalytic DNA: a novel tool for gene suppression.


:RNA, as an intermediate in the production of every gene encoded protein and the genetic material of many pathogenic viruses, presents an attractive target for both biological and therapeutic manipulation. Despite its extensive involvement in living systems, its chemical diversity based on four units is relatively low compared with protein. This provides the opportunity for a generic approach to targeting with specificity based on primary structure rather than complex higher order structures. This form of recognition occurs naturally in complementary nucleic acids, due to an ability to bind their single stranded target through Watson-Crick interactions. The most established nucleic acid based approach to gene suppression at the RNA level is through antisense oligodeoxynucleotides (ODNs). These compounds form heteroduplex with target RNA which are thought to either block its function or mediate its destruction by activation of RNase H. Alternatively, RNA can be targeted by catalytic RNA such as the hammerhead ribozyme. Ribozymes have the advantage of being equipped with their own RNA cleavage apparatus and are therefore independent of host nuclear protein activity. At present, the utility of ribozyme oligonucleotides is restricted by the relative difficulty synthesising active molecules with sufficient resistance to nuclease degradation. Recently the power of in vitro selection has been used to evolve catalytic DNA sequences with RNA cleavage specificity and activity rivalling the very best ribozymes, while maintaining the more robust chemistry of an ODN. These deoxyribozymes or DNAzymes have tremendous potential as gene suppression agents for both target validation and therapeutic applications. A number of studies evaluating the biological activity of these compounds have shown promising results. However, as with other oligonucleotide based strategies, future exploitation of this approach may depend on accessory technology to assist with the accessibility of a target which is folded by its own secondary structure and hidden within the intracellular compartment.


Curr Drug Targets


Current drug targets


Cairns MJ,Saravolac EG,Sun LQ





Has Abstract


2002-06-01 00:00:00












  • Nanotheranostics in evidence based personalized medicine.

    abstract::Efficient drug delivery systems are exceedingly important for novel drug discovery. The evidence-based personalized medicine (EBPM) promises to deliver the right drug at the right time to a right patient as it covers clinicallysignificant genetic predisposition and chronopharmacological aspects of nanotheranostics. Re...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Sharma S

    更新日期:2014-01-01 00:00:00

  • Natural Products as Anticancer Agents.

    abstract::Medicinal plants and mushrooms have alwaysfascinated the world as an attractive source of natural compounds for cancer therapy. From ancient times, they have been valued as gourmet food and folk medicine in Oriental practice. For over 40 years, world has witnessed the overwhelming interest of western scientific frater...

    journal_title:Current drug targets

    pub_type: 杂志文章


    authors: Varghese R,Dalvi YB

    更新日期:2020-12-30 00:00:00

  • Anti-TNF antibody therapy for inflammatory bowel disease during pregnancy: a clinical review.

    abstract::The incidence of inflammatory bowel disease (IBD; Crohn's disease, ulcerative colitis) is highest during the peak reproductive years, hence the increased concern with the safety of IBD drugs during pregnancy. Over the past 11 years, anti-TNF-alpha antibody therapy has emerged as a treatment approach for refractory IBD...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: El Mourabet M,El-Hachem S,Harrison JR,Binion DG

    更新日期:2010-02-01 00:00:00

  • 5-lipoxygenase: a promising drug target against inflammatory diseases-biochemical and pharmacological regulation.

    abstract::5-Lipoxygenase (5-LO) is the key enzyme involved in the synthesis of pro-inflammatory leukotrienes (LTs) and has become a prime target for new drug discovery research and development efforts by the pharmaceutical and biotech industry. The pathophysiological effects of LTs can be modulated by the selective inhibition o...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Anwar Y,Sabir JS,Qureshi MI,Saini KS

    更新日期:2014-04-01 00:00:00

  • Pancreatic Neoplasms and Autophagy.

    abstract::Pancreatic cancer is predicted to be the second deadliest malignancy (a median survival of 4-6 months and a 5-year survival of less than 5%) in the USA by 2020. Although current medical detection technologies have dramatically improved the survival rate for patients with other gastrointestinal malignancies, the dismal...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Barton LA,Ren J

    更新日期:2018-01-01 00:00:00

  • Study of Monocytes/Macrophages Stimuli as the Targets of Treating Inflammatory Bone Diseases.

    abstract::Inflammation is the most common pathology in many orthopedic diseases, such as: rheumatoid arthritis (RA), osteoarthritis (OA) and other reasons caused osteolysis. The leading factor of inflammation was considered as the differentiation of monocyte and the polarization of macrophage. However, cytokines and different c...

    journal_title:Current drug targets

    pub_type: 杂志文章


    authors: Liu Y,Cao Y,Smith W,He B,Yan L

    更新日期:2020-01-01 00:00:00

  • Triterpene derivatives as inhibitors of protein involved in the inflammatory process: molecules interfering with phospholipase A2, cycloxygenase, and lipoxygenase.

    abstract::Over the past years, there was an explosion in the knowledge of the protein target and molecular mechanism associated with various disease types and in the new research of drugs of natural origin. The key idea is to evaluate bioactive natural products interacting with protein domains of different genetic origin but st...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Braca A,Dal Piaz F,Marzocco S,Autore G,Vassallo A,De Tommasi N

    更新日期:2011-03-01 00:00:00

  • The therapeutic value of natural agents to treat miRNA targeted breast cancer in African-American and Caucasian-American women.

    abstract::Breast cancer is the most common cancer in women in the United States, with African-American (AA) women showing significantly higher rates than Caucasian-American (CA) women do. The reason for this racial disparity remains unknown, and factors that might be responsible for the differences in incidence and mortality ha...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Rahman KM,Sakr WA

    更新日期:2012-12-01 00:00:00

  • Non-steroidal anti-inflammatory drugs and cyclooxygenase in Alzheimer's disease.

    abstract::Epidemiological studies indicate that anti-inflammatory drugs, especially the non-steroidal anti-inflammatory drugs (NSAIDs), decrease the risk of developing Alzheimer's disease (AD). Their beneficial effects may be due to interference in the chronic inflammatory reaction, that takes place in AD. The best-characterize...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Hoozemans JJ,Veerhuis R,Rozemuller AJ,Eikelenboom P

    更新日期:2003-08-01 00:00:00

  • Nutrition in adult patients with inflammatory bowel disease.

    abstract::Seventy five percent of hospitalized patients with Crohn's disease suffer from malnutrition. One third of Crohn's disease patients have a body mass index below 20. Sixty percent of Crohn's disease patients have sarcopenia. However some inflammatory bowel disease (IBD) patients are obese or suffer from sarcopenic-obesi...

    journal_title:Current drug targets

    pub_type: 杂志文章


    authors: Hebuterne X,Filippi J,Schneider SM

    更新日期:2014-01-01 00:00:00

  • Oxidative stress: meeting multiple targets in pathogenesis of diabetic nephropathy.

    abstract::Excessive production of reactive oxygen species is an important mechanism underlying the pathogenesis of diabetes associated macrovascular and microvascular complications including diabetic nephropathy. Diabetic nephropathy is characterized by glomerular enlargement, early albuminuria and progressive glomerulosclerosi...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Arora MK,Singh UK

    更新日期:2014-05-01 00:00:00

  • How can we Preserve Sexual Function after Ablative Surgery for Benign Prostatic Hyperplasia?

    abstract::Our aim was to provide a narrative review regarding the prevalence, the associated pathophysiologic pathways and the potential management methods of sexual dysfunction related to ablative surgical techniques for Benign Prostatic Enlargement (BPE). Men suffering from BPE are at high risk of sexual dysfunction due to th...

    journal_title:Current drug targets

    pub_type: 杂志文章


    authors: Mykoniatis I,Renterghem KV,Sokolakis I

    更新日期:2021-01-01 00:00:00

  • Colloidal carrier systems for transcutaneous immunization.

    abstract::Recently, the skin has emerged as a potential alternative route for non-invasive delivery of vaccine. It has been recognized as a highly immune-reactive tissue containing an abundance of antigen presenting cells, especially within the epidermis. Transcutaneous immunization, introduction of antigen through topical appl...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Gupta PN,Vyas SP

    更新日期:2011-04-01 00:00:00

  • Osteopontin; as a target molecule for the treatment of inflammatory diseases.

    abstract::It has been well recognized that inflammatory responses are part of pathogenesis for various disorders such as autoimmune diseases. For example, multiple sclerosis (MS) is an inflammatory demyelinating disease of central nervous system that is presumably caused by activated T cells specific for myelin antigens. Rheuma...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Morimoto J,Kon S,Matsui Y,Uede T

    更新日期:2010-04-01 00:00:00

  • Nitrite-stimulated Gastric Formation of S-nitrosothiols As An Antihypertensive Therapeutic Strategy.

    abstract::Hypertension is usually associated with deficient nitric oxide (NO) bioavailability, and therefore stimulating NO activity is an important antihypertensive strategy. Recently, many studies have shown that both nitrite and nitrate anions are not simple products of NO metabolism and indeed may be reduced back to NO. Whi...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Oliveira-Paula GH,Tanus-Santos JE

    更新日期:2019-01-01 00:00:00

  • Drug-binding databases.

    abstract::Recent developments in computer power and chemoinformatics methodology make possible that a huge amount of data become available through internet. These databases are devoted to a wide spectrum of scientific fields. Here we are concerned with databases related to protein-drug interactions. More specifically, databases...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Timmers LF,Pauli I,Caceres RA,de Azevedo WF Jr

    更新日期:2008-12-01 00:00:00

  • Targeting glycogen synthase kinase-3 in the CNS: implications for the development of new treatments for mood disorders.

    abstract::There exists an immediate need to develop novel medications for the treatment of mood disorders such as bipolar disorder and depression. Initial interest in glycogen synthase kinase-3 (GSK-3) as a target for the treatment of mood disorders arose from the finding that the mood stabilizing drug lithium directly inhibite...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Gould TD,Picchini AM,Einat H,Manji HK

    更新日期:2006-11-01 00:00:00

  • Recognition of Plasminogen Activator Inhibitor Type 1 as the Primary Regulator of Fibrinolysis.

    abstract::The fibrinolytic system consists of a balance between rates of plasminogen activation and fibrin degradation, both of which are finely regulated by spatio-temporal mechanisms. Three distinct inhibitors of the fibrinolytic system that differently regulate these two steps are plasminogen activator inhibitor type-1 (PAI-...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Urano T,Suzuki Y,Iwaki T,Sano H,Honkura N,Castellino FJ

    更新日期:2019-01-01 00:00:00

  • PHB in Cardiovascular and Other Diseases: Present Knowledge and Implications.

    abstract:BACKGROUND:Prohibitin (PHB) is overtly conserved evolutionarily and ubiquitously expressed protein with pleiotropic functions in diverse cellular compartments. However, regulation and function of these proteins in different cells, tissues and in various diseases is different as evidenced by expression of these proteins...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Chowdhury D,Kumar D,Sarma P,Tangutur AD,Bhadra MP

    更新日期:2017-11-30 00:00:00

  • Osteoarthritis: aging of matrix and cells--going for a remedy.

    abstract::It has been known for a very long time that aging is the most prominent risk factor for the initiation and progression of osteoarthritis. This might be related to continuous mechanical wear and tear and/or result from time/age-related modifications of cartilage matrix components. Also a mere loss of viable cells over ...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Aigner T,Haag J,Martin J,Buckwalter J

    更新日期:2007-02-01 00:00:00

  • Decreasing the metastatic potential in cancers--targeting the heparan sulfate proteoglycans.

    abstract::The heterogeneity of proteoglycans (PG)s contributes to their functional diversity. Many functions depend on their ability to bind and modulate the activity of components of the extracellular matrix (ECM). The ability of PGs to interact with other molecules, such as growth factors, is largely determined by the fine st...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Fjeldstad K,Kolset SO

    更新日期:2005-09-01 00:00:00

  • Metal and inflammatory targets for Alzheimer's disease.

    abstract::Alzheimer's disease (AD) has become linked to inflammation and metal biology. Metals (copper, zinc and iron) and inflammatory cytokines are significant factors that increase the onset of sporadic late onset forms of the dementia. The genetic discovery that alleles in the hemochromatosis gene accelerate the onset of di...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Rogers JT,Lahiri DK

    更新日期:2004-08-01 00:00:00

  • Ion-Responsive Drug Delivery Systems.

    abstract:BACKGROUND:Some kinds of cations and anions are contained in body fluids such as blood, interstitial fluid, gastrointestinal juice, and tears at relatively high concentration. Ionresponsive drug delivery is available to design the unique dosage formulations which provide optimized drug therapy with effective, safe and ...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Yoshida T,Shakushiro K,Sako K

    更新日期:2018-02-08 00:00:00

  • Molecular pathology of pancreatic cancer: from bench-to-bedside translation.

    abstract::Pancreatic ductal adenocarcinoma (referred here as pancreatic cancer) is a lethal disease with the worst prognosis among all solid tumors. Surgical resection represents the only hope for cure but it is possible only in patients that present with local disease (about 20% of cases). Whether dismal prognosis of pancreati...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Corbo V,Tortora G,Scarpa A

    更新日期:2012-06-01 00:00:00

  • Targeting c-MET/HGF signaling pathway in upper gastrointestinal cancers: rationale and progress.

    abstract::Aberrant activation of receptor-tyrosine kinase c-Met/HGF pathway is shown to be associated with cell proliferation, invasion, metastasis and poor-prognosis in several tumor types, including upper gastrointestinal-malignancies. The interaction of c-Met with multiple signalling-pathways involved in tumorigenic-properti...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Gholamin S,Fiuji H,Maftouh M,Mirhafez R,Shandiz FH,Avan A

    更新日期:2014-01-01 00:00:00

  • Novel imaging techniques in acute kidney injury.

    abstract::Imaging of the kidneys can provide valuable information in the work up and management of acute kidney injury. Several different imaging modalities are used to gather information on anatomy of the kidney, to rule out obstruction, differentiate acute kidney injury (AKI) and chronic kidney disease and to obtain informati...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Kalantarinia K

    更新日期:2009-12-01 00:00:00

  • Virally encoded G protein-coupled receptors: targets for potentially innovative anti-viral drug development.

    abstract::Various herpes- and poxviruses contain DNA sequences encoding proteins with homology to cellular chemokine receptors, which belong to the family of G protein-coupled receptors (GPCRs). Since GPCRs play a crucial role in cellular communication and chemokine receptors play a prominent role in the immune system, the vira...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Smit MJ,Vink C,Verzijl D,Casarosa P,Bruggeman CA,Leurs R

    更新日期:2003-07-01 00:00:00

  • An Overview of Prospective Drugs for Type 1 and Type 2 Diabetes.

    abstract:AIMS:The aim of this study is to provide an overview of several emerging anti-diabetic molecules. BACKGROUND:Diabetes is a complex metabolic disorder involving the dysregulation of glucose homeostasis at various levels. Insulin, which is produced by β-pancreatic cells, is a chief regulator of glucose metabolism, regul...

    journal_title:Current drug targets

    pub_type: 杂志文章


    authors: Wu P,Liu Z,Jiang X,Fang H

    更新日期:2020-01-01 00:00:00

  • Central oxytocinergic neurotransmission: a drug target for the therapy of psychogenic erectile dysfunction.

    abstract::A group of oxytocinergic neurons originating in the paraventricular nucleus of the hypothalamus and projecting to extrahypothalamic brain areas (e.g. hippocampus, medulla oblongata and spinal cord) control penile erection. Activation of these neurons by dopamine and dopamine agonists, excitatory amino acids (N-methyl-...

    journal_title:Current drug targets

    pub_type: 杂志文章,评审


    authors: Melis MR,Argiolas A

    更新日期:2003-01-01 00:00:00

  • Pharmacological treatments for neovascular age-related macular degeneration: can mixed treatment comparison meta-analysis be useful?

    abstract:OBJECTIVE:To investigated the use of mixed treatment comparison (MTC) meta-analysis models to summarize results from randomized clinical trials (RCTs) on approved pharmacological treatments for neovascular age-related macular degeneration (AMD). METHODS:The number of patients with visual loss or visual gain of 3 or mo...

    journal_title:Current drug targets

    pub_type: 杂志文章,meta分析,评审


    authors: Virgili G,Novielli N,Menchini F,Murro V,Giacomelli G

    更新日期:2011-02-01 00:00:00