Phylogenetic diversity of numerically important Arctic sea-ice bacteria cultured at subzero temperature.

Abstract:

:Heterotrophic bacteria in sea ice play a key role in carbon cycling, but little is known about the predominant players at the phylogenetic level. In a study of both algal bands and clear ice habitats within summertime Arctic pack ice from the Chukchi Sea, we determined the abundance of total bacteria and actively respiring cells in melted ice samples using epifluorescence microscopy and the stains 4', 6'-diamidino-2-phenylindole 2HCl (DAPI) and 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), respectively. Organic-rich and -poor culturing media were used to determine culturable members by plating (at 0 degrees C and 5 degrees C) and most-probable-number (MPN) analyses (at -1 degrees C). Total bacterial counts ranged from 5.44 x 10(4) ml(-1) in clear ice to 2.41 x 10(6) ml(-1) in algal-band ice samples, with 2-27% metabolically active by CTC stain. Plating and MPN results revealed a high degree of culturability in both types of media, but greater success in oligotrophic media (to 62% of total abundance) and from clear ice samples. The bacterial enumeration anomaly, commonly held to mean

journal_name

Microb Ecol

journal_title

Microbial ecology

authors

Junge K,Imhoff F,Staley T,Deming JW

doi

10.1007/s00248-001-1026-4

keywords:

subject

Has Abstract

pub_date

2002-04-01 00:00:00

pages

315-28

issue

3

eissn

0095-3628

issn

1432-184X

journal_volume

43

pub_type

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