Neuronal co-localization of different isoforms of tachykinin-related peptides (LemTRPs) in the cockroach brain.

Abstract:

:Seven isoforms of tachykinin-related peptides (TRPs) have been isolated from the brain of the cockroach Leucophaea maderae. These peptides (LemTRP-1, 2, and 5-9) share the C-terminal sequence GFX(1)GX(2)Ramide (where X(1) and X(2) are variable residues). In order to determine the neuronal distribution of several of these LemTRP isoforms, we raised antisera to their variable N-termini. Antisera to LemTRP-1, 2, 3, 7, and 8 were utilized for immunocytochemistry on cryostat sections of the L. maderae brain. As expected, the gut peptide LemTRP-3 was not detected in the brain, and the antisera to LemTRP-1, 2, and 7 labeled the same sets of neurons in different regions of the brain. These neurons could also be labeled with antisera raised to the more conserved C-termini of LemTRP-1 and the locust TRP LomTK-I. The antiserum to LemTRP-8 predominantly labeled a set of neurons distinct from that seen with any other N- or C-terminus-directed antisera, suggesting that it recognizes epitope(s) other than known insect TRPs. Our findings indicate that at least three of the LemTRPs are always co-localized in neurons of the L. maderae brain. We have also been able to show that LemTRP-2, which is an N-terminally extended form (17-mere) of LemTRP-1 with a dibasic putative cleavage site, is transported throughout the processes of the neurons in the same manner as LemTRP-1 and 7. Thus, LemTRP-2 may be released with the other shorter LemTRPs. This is the first investigation of LemTRP distribution in the cockroach central nervous system utilizing antisera to native peptides.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Nässel DR,Winther AM

doi

10.1007/s00441-002-0538-7

keywords:

subject

Has Abstract

pub_date

2002-05-01 00:00:00

pages

225-39

issue

2

eissn

0302-766X

issn

1432-0878

journal_volume

308

pub_type

杂志文章
  • Distribution of the extended family of protein kinase C isoenzymes in fetal organs of mice: an immunohistochemical study.

    abstract::Using isoenzyme-specific antisera, we have studied the distribution of protein kinase C isoforms in fetal mouse organs at the developmental age of 17 days. Two different sets of antibodies, produced by different manufacturers, were employed in this study. The specificity of the antisera was tested by immunoblotting ex...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00318364

    authors: Bareggi R,Grill V,Zweyer M,Narducci P,Martelli AM

    更新日期:1995-06-01 00:00:00

  • The ultrastructure of the mid-gut cells of Nasonia vitripennis (Walker) (Hymenoptera, Pteromalidae).

    abstract::The ultrastructure of the mid-gut cells of female Nasonia vitripennis is described. The mid-gut consists of a uniform, single-cell epithelium. The cells of different gut regions were analysed using morphometric techniques in order to determine any differences in the components. The structure of the cells is described ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00221084

    authors: Davies I,King PE

    更新日期:1977-02-09 00:00:00

  • Immunohistochemical demonstration of TSH-, LH- and ACTH-cells in the hypophysis of tadpoles of Xenopus laevis D.

    abstract::By use of the immunofluorescence technique TSH-, LH- and ACTH-cells were localized in the hypophysis of tadpoles of Xenopus laevis. The first signs of the activity of these cells were observed in early stages of the development, i.e., stage 39 for ACTH, and stage 42 for TSH and LH. ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00216218

    authors: Moriceau-Hay D,Doerr-Schott J,Dubois MP

    更新日期:1982-01-01 00:00:00

  • Immune complexes of E. coli antigens and maternal IgG in the bursa of Fabricius.

    abstract::The bursa of Fabricius of the chicken is known to be both a primary lymphoid organ and a secondary lymphoid tissue. Bursal follicles are equipped with antigen-trapping follicle-associated epithelium. However, bioactive antigens such as protein and bacteria have not been detected in the bursal parenchyma. By immunopero...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-013-1701-z

    authors: Sonoda K,Noguchi K,Ekino S

    更新日期:2013-12-01 00:00:00

  • Proliferating cell nuclear antigen (PCNA/cyclin) immunocytochemistry as a labeling index in mouse lung tissues.

    abstract::Proliferating cell nuclear antigen is expressed in cells from late G1 through the S-phase of the cell cycle. Therefore, antibodies directed against this molecule should provide a probe for labeling immunocytochemically the nuclei of proliferating cells. Herein we demonstrate the feasibility and reliability of this tec...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00224731

    authors: Thaete LG,Ahnen DJ,Malkinson AM

    更新日期:1989-04-01 00:00:00

  • Homing of germinal-center cells into germinal centers of lymph node via afferent lymphatics. An autoradiographic study in rabbits.

    abstract::Affinity of lymphoid cells for the microenvironment of germinal centers (GC), as detectable in transfer experiments by rapid homing in spleen GC from the blood, is a capacity expressed by only a subset of lymphoid cells, in particular by those constituting a GC. However, when introduced into the blood stream, these ce...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00215160

    authors: Deenen GJ,Opstelten D,Nieuwenhuis P

    更新日期:1984-01-01 00:00:00

  • Changes in retinal neuronal populations in the DBA/2J mouse.

    abstract::DBA/2J (D2) mice develop a form of progressive pigmentary glaucoma with increasing age. We have compared retinal cell populations of D2 mice with those in control C57BL/6J mice to provide information on retinal histopathology in the D2 mouse. The D2 mouse retina is characterized by a reduction in retinal thickness cau...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-004-1062-8

    authors: Moon JI,Kim IB,Gwon JS,Park MH,Kang TH,Lim EJ,Choi KR,Chun MH

    更新日期:2005-04-01 00:00:00

  • Duplication of a peripheral sensory neuron in the cockroach Periplaneta americana.

    abstract::We have recently examined the electrophysiology and ultrastructure of approximately 100 tactile spines from the metathoracic legs of adult cockroaches. In only one animal the single sensory neuron that innervates the spine was replaced with a pair of apparently identical neurons which we believe were both functional. ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00216522

    authors: Kuster JE,French AS

    更新日期:1984-01-01 00:00:00

  • Moulting hormone, juvenile hormone and the ultrastructure of the fat body of adult Sarcophaga bullata (Diptera).

    abstract::In the ovoviviparous fly, Sarcophaga bullata, vitellogenesis in cyclic; a process reflected in ultrastructural changes in the fat body cells and oenocytes. At eclosion the larval fat body has not yet completely disappeared. During vitellogenesis the fat body cells are specialized for intensive protein synthesis showin...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00216728

    authors: Stoppie P,Briers T,Huybrechts R,De Loof A

    更新日期:1981-01-01 00:00:00

  • Immunocytochemical analysis of misplaced rhodopsin-positive cells in the developing rodent retina.

    abstract::During the first postnatal weeks of the developing rodent retina, rhodopsin can be detected in a number of neuron-like cells in the inner retina. In the present study, we aim to characterize the morphology, number and staining characteristics of this peculiar population. Misplaced rhodopsin-positive cells (MRCs) were ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-013-1788-2

    authors: Szabó K,Szabó A,Enzsöly A,Szél A,Lukáts A

    更新日期:2014-04-01 00:00:00

  • Serum-free cryopreservation of porcine hepatocytes.

    abstract::The use of porcine hepatocytes in xenotransplantation, bioartificial liver support or pharmacological approaches demands serum-free cryopreservation protocols yielding high quality, viable, functional hepatocytes. Here, primary porcine hepatocytes were frozen without serum in liquid nitrogen by the use of a computer-a...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-004-0894-6

    authors: Müller P,Aurich H,Wenkel R,Schäffner I,Wolff I,Walldorf J,Fleig WE,Christ B

    更新日期:2004-07-01 00:00:00

  • Scanning electron microscopic study of spermatozoa from gossypol-treated rats.

    abstract::Spermatozoa from the cauda epididymidis of gossypol-treated rats exhibit distinctive departures from the morphology of spermatozoa from control rats: wrinkled and disorganized cell membrane in the head and tail regions, cell membrane missing from segments of the tail midpiece and principal piece regions, malformed hea...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00210003

    authors: Bozek SA,Jensen DR,Tone JN

    更新日期:1981-01-01 00:00:00

  • Ultrastructural changes in the spiral limbus associated with carboplatin-induced ablation of inner hair cells.

    abstract::Four months after the selective ablation of inner hair cells by carboplatin, the interdental cell epithelium exhibited dilated intercellular spaces and cytosolic vacuoles not seen in controls. In addition, the wide, often electron-lucent phalanges observed in the interdental cells of the normal chinchilla collapsed in...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410000253

    authors: Spicer SS,Salvi RJ,Schulte BA

    更新日期:2000-10-01 00:00:00

  • Carbohydrate-binding proteins of tumor lines with different growth properties. II. Changes in their pattern in clones of transformed rat fibroblasts of differing metastatic potential.

    abstract::A tumor model system of clones of myeloproliferative sarcoma virus (MPV)-transformed rat fibroblasts (NRK) with different growth properties and metastatic potential was studied. The relationship between metastatic behavior and composition of carbohydrate-binding proteins (lectins) was analyzed by affinity chromatograp...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00215191

    authors: Gabius HJ,Vehmeyer K,Engelhardt R,Nagel GA,Cramer F

    更新日期:1986-01-01 00:00:00

  • The fine structure of polychaete septate junctions.

    abstract::Epidermal septate junctions of Nereis sp. and Cirriformia sp. fixed with OsO4 or glutaraldehyde/OsO4 display variable structure in electron micrographs. In transverse section the septa are often indistinct and obscured by opaque material that fills the junctional cleft. Septa (spaced at 180--280 A) are more clearly de...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00222150

    authors: Baskin DG

    更新日期:1976-10-22 00:00:00

  • Daily fluctuations of nucleoli in neurosecretory cells of the rat supraoptic nucleus. An ultrastructural and stereological study.

    abstract::Daily fluctuations of nucleoli and nucleolar fibrillar centres in neurosecretory cells from the supraoptic nucleus (SON) were investigated in rats artificially synchronized for 3 weeks to a set 12 h light/12 h dark cycle with free access to food and water. Group of 3 animals were sacrificed by intracardiac perfusion e...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00222351

    authors: Bessone R,Seïte R

    更新日期:1985-01-01 00:00:00

  • Immunocytochemical demonstration of neuropeptides and serotonin in the tapeworm Diphyllobothrium dendriticum.

    abstract::The present immunocytochemical study concerns the distribution of four neuropeptides, FMRF-amide, vasotocin, leu-enkephalin and neurotensin, and of the bioamine serotonin in the plerocercoid larva of Diphyllobothrium dendriticum. Anti-FMRF-amide and vasotocin-reactivity occurs in perikarya and nerve fibres in the CNS ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00222332

    authors: Gustafsson MK,Wikgren MC,Karhi TJ,Schot LP

    更新日期:1985-01-01 00:00:00

  • The passage of macrophage and lymphocytes from the interstitium across the lymphatic endothelium of rat lacteals.

    abstract::The passage of cells across the lymphatic endothelium of rat lacteals in both normal and non-pathological experimental conditions (fasting, lymphatic, stasis) was studied by means of serial thin sections and three-dimensional models. Two different pathways of transendothelial migration were observed: (1) macrophages e...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00327761

    authors: Azzali G

    更新日期:1990-10-01 00:00:00

  • Effects of background adaptation on alpha-MSH and beta-endorphin in secretory granule types of melanotrope cells of Xenopus laevis.

    abstract::Placing the clawed toad Xenopus laevis on a black background stimulates the melanotrope cells in the pars intermedia of the pituitary gland to release proopiomelanocortin (POMC)-derived peptides, including alpha-MSH and N-acetyl-beta-endorphin. In this study three types of secretory granules, electron-dense (approxima...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00314557

    authors: Roubos EW,Berghs CA

    更新日期:1993-12-01 00:00:00

  • Effects of the actin-binding protein DNAase I on cytoplasmic streaming and ultrastructure of Amoeba proteus. An attempt to explain amoeboid movement.

    abstract::Microinjection of DNAase I, which is known to form a specific complex with G-actin, induces characteristic changes in cytoplasmic streaming, locomotion and morphology of the contractile apparatus of A. proteus. Light microscopical studies show pronounced streaming originating from the uroid and/or the retracting pseud...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00236075

    authors: Wehland J,Weber K,Gawlitta W,Stockem W

    更新日期:1979-07-17 00:00:00

  • Protective effects of the neuropeptide PACAP in diabetic retinopathy.

    abstract::Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with highly potent neurotrophic and neuroprotective effects. PACAP and its receptors occur in the retina and PACAP has been applied in animal models of metabolic retinal disorders to reduce structural and functional damage. Furthermore, PACAP...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-012-1349-0

    authors: Szabadfi K,Atlasz T,Kiss P,Reglodi D,Szabo A,Kovacs K,Szalontai B,Setalo G Jr,Banki E,Csanaky K,Tamas A,Gabriel R

    更新日期:2012-04-01 00:00:00

  • Immunocytological and FISH analysis of pole cell formation and soma elimination of germ line-limited chromosomes in the chironomid Acricotopus lucidus.

    abstract::In the chironomid Acricotopus lucidus, germ line-soma differentiation becomes evident with the formation of the pole cells and the elimination of the germ line-limited chromosomes (Ks) from the future somatic nuclei of the embryo. Unlike in Drosophila, the early nuclear divisions do not proceed synchronously in A. luc...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410000270

    authors: Staiber W

    更新日期:2000-11-01 00:00:00

  • Light- and electron-microscopic study of the distribution of axons containing substance P and the localization of neurokinin-1 receptor in bone.

    abstract::Substance P (SP) is a neuropeptide that is released from axons of sensory neurons and causes signal transduction through the activation of the neurokinin-1 receptor (NK1-R). The present study demonstrates the distribution of SP-like-immunoreactive (SP-LI) axons and the localization of NK1-Rs in rat bone tissue using t...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410051100

    authors: Goto T,Yamaza T,Kido MA,Tanaka T

    更新日期:1998-07-01 00:00:00

  • The histological architecture of the auditory organs in the parasitoid fly Ormia ochracea.

    abstract::Gravid females of the parasitoid fly species Ormia ochracea (Diptera: Tachinidae) rely on their sense of hearing to detect and localize their hosts, field crickets of the genus Gryllus. As in any hearing insect species, the fly's auditory system consists of a rather complex cascade of mechanical and physiological mech...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410000257

    authors: Robert D,Willi U

    更新日期:2000-09-01 00:00:00

  • Histological and cytological studies on the fat body of the cockroach Nauphoeta cinerea during the first reproductive cycle.

    abstract::The central fat body of the ovoviviparous cockroach Nauphoeta cinerea was studied during the first reproductive cycle of the female by means of light microscopy, autoradiography and electron microscopy. Comparative studies in larval stages were also undertaken. The fat body of Nauphoeta contains a large amount of lipi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00219785

    authors: Wüest J

    更新日期:1978-04-28 00:00:00

  • Spatial and temporal regulation of glycosylation during Drosophila eye development.

    abstract::Glycosylation plays an essential role during development, in processes such as morphogen distribution, cell-to-cell communication, and extracellular matrix formation. Glycosylation is regulated during development in both a spatial and temporal manner. This study presents a detailed description of glycan distribution f...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-009-0753-6

    authors: Yano H,Yamamoto-Hino M,Goto S

    更新日期:2009-04-01 00:00:00

  • Incorporation of L-(3H)-fucose into glycoproteins of the adrenal gland of mice. Light microscope radioautographic study on semi-thin sections.

    abstract::To study the biosynthesis and intracellular migration of glycoproteins in the adrenal gland, adult mice were injected intravenously with L-(3H)-fucose and killed from 10 min to 14 days after injection. Semi-thin sections of the adrenal glands were then processed for radioautography. Incorporation of labeled fucose occ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00232246

    authors: Haddad A,Brasileiro IL,Pelletier G

    更新日期:1979-11-01 00:00:00

  • Improved visualization of the immunoreactive hypothalamo-neurohypophysial system by use of immuno-gold techniques.

    abstract::Ultrastructural post-embedding immuno-gold techniques were applied to the supraoptic nucleus and the neurohypophysis of mice and rats. The primary antibodies were three different monoclonal antineurophysins, used in protein A-gold and immunoglobulin-gold procedures. Conventional plastic embedding as well as hydrophili...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00221868

    authors: Castel M,Morris JF,Whitnall MH,Sivan N

    更新日期:1986-01-01 00:00:00

  • Male-associated polypeptide (MAP) expression in different compartments of the reproductive system of the mussel Mytilus galloprovincialis: immunocytochemical and western blot study.

    abstract::Mytilus mussels are characterized by annually repeated reproduction which is associated with subsequent growth, morphogenesis, breakdown and redevelopment of the gonad and reproductive tract into mantle mesenchyme. We present a description of the expression of the male-associated polypeptide (MAP; see Mikhailov et al....

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410051204

    authors: Torrado M,Mikhailov AT

    更新日期:1998-12-01 00:00:00

  • Uteroglobin-like antigen in the pulmonary epithelium and secretion of the lung.

    abstract::The immunological identity of a uteroglobin-like protein, occurring in respiratory tract secretions and tissue, with uteroglobin from rabbit endometrial secretion is demonstrated. A uteroglobin-like antigen has been localized in bronchial epithelial cells and in bronchioles by immunofluorescence. This secretory protei...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00210033

    authors: Beier HM,Kirchner C,Mootz U

    更新日期:1978-06-26 00:00:00