The Escherichia coli RNA degradosome: structure, function and relationship in other ribonucleolytic multienzyme complexes.

Abstract:

:mRNA instability is an intrinsic property that permits timely changes in gene expression by limiting the lifetime of a transcript. The RNase e of Escherichia coli is a single-strand-specific endo-nuclease involved in the processing of rRNA and the degradation of mRNA. A nucleolytic multi-enzyme complex now known as the RNA degradosome was discovered during the purification and characterization of RNase E. Two other components are a 3' exoribonuclease (polynucleotide phosphorylase, PNPase) and a DEAD-box RNA helicase (RNA helicase B, RhlB). RNase E is a large multidomain protein with N-terminal ribonucleolytic activity, an RNA-binding domain and a C-terminal "scaffold" that binds PNPase, enolase and RhlB. RhlB by itself has little activity but is strongly stimiulated by its interaction with RNase E. RhlB in vitro can facilitate the degradation of structured RNA by PNPase. Since the discovery of the RNA degradosome in E. coli, related complexes have been described in other organisms.

journal_name

Biochem Soc Trans

authors

Carpousis AJ

keywords:

subject

Has Abstract

pub_date

2002-04-01 00:00:00

pages

150-5

issue

2

eissn

0300-5127

issn

1470-8752

journal_volume

30

pub_type

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