Use of insulin to improve [18 F]fluorodeoxyglucose labelling and retention for in vivo positron emission tomography imaging of monocyte trafficking.


:While 18F-FDG labelling of monocytes would allow in vivo trafficking with positron emission tomography (PET), present methods suffer from poor retention of radioactivity. We investigated the feasibility of utilizing insulin for improved [18F]fluorodeoxyglucose (18F-FDG) labelling. Separated human monocytes and lymphocytes were labelled with 18F-FDG with or without 3 h insulin pre-incubation. Insulin had no effect on lymphocyte labelling (21.4+/-0.8% vs 20.8+/-1.1% efficiency, P=NS). However, for monocytes, insulin pre-incubation led to a 169+/-9% increase in labelling efficiency (19.3+/-4.1 vs 32.5+/-1.8, P<0.05), without significant effects on cell activation or viability. Moreover, while only 57.7+/-4.8% and 40.4+/-5.6% of the 18F-FDG was retained at 1 and 3 h for controls, the retention rate increased to 91.6+/-2.1% (P=0.01) and 86.5+/-1.9% (P<0.01) after insulin pre-incubation. Improved 18F-FDG retention was accompanied by a 70.3+/-7.4% decrease in glucose-6-phosphatase activity (P=0.02). PET imaging of rats showing hepatic ischaemia-reperfusion injury demonstrated higher liver uptake for monocytes labelled after insulin treatment. Thus, insulin improves monocytic 18F-FDG uptake and retention, and may provide a feasible labelling method for PET imaging.


Nucl Med Commun


Paik JY,Lee KH,Byun SS,Choe YS,Kim BT





Has Abstract


2002-06-01 00:00:00












  • Evaluating the use of optimally respiratory gated 18F-FDG-PET in target volume delineation and its influence on radiation doses to the organs at risk in non-small-cell lung cancer patients.

    abstract:OBJECTIVE:This radiotherapy planning study evaluated tumour delineation using both optimally respiratory gated and nongated fluorine-18 fluorodeoxyglucose-PET (F-FDG-PET). METHODS:For 22 non-small-cell lung tumours, both scans were used to create the nongated and gated (g) gross tumour volumes (GTVg) together with the...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Wijsman R,Grootjans W,Troost EG,van der Heijden EH,Visser EP,de Geus-Oei LF,Bussink J

    更新日期:2016-01-01 00:00:00

  • No-carrier-added 123I-MIBG: an initial clinical study in patients with phaeochromocytoma.

    abstract::Radioiodinated meta-iodobenzylguanidine (MIBG) is used routinely for imaging and targeted radiotherapy of tumours derived from the neural crest. Since active uptake of MIBG by the noradrenaline transporter (NAT) makes a greater contribution to total drug accumulation than passive uptake when MIBG is present at low con...

    journal_title:Nuclear medicine communications

    pub_type: 临床试验,杂志文章


    authors: Owens J,Bolster AA,Prosser JE,Cunningham S,Mairs RJ,Neilly JB,Reed NS,Hilditch TE

    更新日期:2000-05-01 00:00:00

  • Optimal buffer choice of the radiosynthesis of (68)Ga-Dotatoc for clinical application.

    abstract:OBJECTIVES:(68)Ga-Dotatoc has become the PET radiopharmaceutical of choice for the diagnosis and treatment follow-up of neuroendocrine tumours. (68)Ga-Dotatoc is prepared on-site through a so-called magisterial preparation. The use of an appropriate buffer during the radiolabelling step is essential to maximize the lab...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Bauwens M,Chekol R,Vanbilloen H,Bormans G,Verbruggen A

    更新日期:2010-08-01 00:00:00

  • Metastatic differentiated thyroid carcinoma: clinical management and outcome of disease in patients with initial and late distant metastases.

    abstract:OBJECTIVE:Our objective was to analyze the clinical management and the outcome of disease in differentiated thyroid carcinoma with initial and late distant metastases (M1). PATIENTS AND METHODS:Radioiodine (131I) therapy was applied in 77 differentiated thyroid carcinoma patients with M1 (31 patients with M1-initial a...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Mihailovic JM,Stefanovic LJ,Malesevic MD,Erak MD,Tesanovic DD

    更新日期:2009-07-01 00:00:00

  • Relationship between positive thyroglobulin doubling time and 18F-FDG PET/CT-positive, 131I-negative lesions.

    abstract:AIM:In patients with differentiated thyroid carcinoma (DTC), metastases can either show iodine-131 (I) uptake on whole-body scintigraphy or F-2-fluoro-2-deoxy-D-glucose (F-FDG) uptake on combined PET and X-ray computed tomography (PET/CT), or a mix of both. The present study investigates the relationship between uptake...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Kelders A,Kennes LN,Krohn T,Behrendt FF,Mottaghy FM,Verburg FA

    更新日期:2014-02-01 00:00:00

  • Extraction of arterial input function for measurement of brain perfusion index with 99mTc compounds using fuzzy clustering.

    abstract::Cerebral blood flow (CBF) can be quantified non-invasively using the brain perfusion index (BPI) determined from radionuclide angiographic data generated with 99mTc-hexamethylpropylene amine oxime (99mTc-HMPAO). When measuring the BPI, manual drawing of regions of interest (ROIs) (manual ROI method) for the extraction...

    journal_title:Nuclear medicine communications

    pub_type: 临床试验,杂志文章


    authors: Murase K,Nagayoshi M,Uenishi Y,Kawamata M,Yamazaki Y,Takasawa M,Oku N,Hatazawa J

    更新日期:2004-03-01 00:00:00

  • Excretion and whole-body retention of radium-223 dichloride administered for the treatment of bone metastases from castration resistant prostate cancer.

    abstract:OBJECTIVE:The aim of the study was to determine the fraction of administered activity that was excreted and retained by a small cohort of patients who each received treatment with radium-223 dichloride (Ra). Ra is an α-emitting radionuclide that has been approved for use in the treatment of bone metastases that are sec...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Pratt BE,Hindorf C,Chittenden SJ,Parker CC,Flux GD

    更新日期:2018-02-01 00:00:00

  • Different fibrovascularization rate between coralline hydroxyapatite and high density porous polyethylene (Medpore) measured by 99mTc-MDP bone scintigraphy 6 months after intraorbital implantation.

    abstract::Many materials and types of implant have been used to achieve a cosmetic effect and prosthesis motility in the anophthalmic socket. Hydroxyapatite remains the implant material of choice for producing the most natural prosthesis motility while porous polyethylene shows promising characteristics as another useful materi...

    journal_title:Nuclear medicine communications

    pub_type: 临床试验,杂志文章


    authors: Pan MH,Wu YW,Yen RF,Tzen KY,Liao SL,Kao CH

    更新日期:2003-12-01 00:00:00

  • Aspirin renography in the diagnosis of renovascular hypertension: a comparative study with captopril renography.

    abstract::Captopril renography is used for the non-invasive diagnosis of renovascular hypertension, but suffers from the drawbacks of lower sensitivity and false-positive tests due to a fall in blood pressure. Aspirin renography has been proposed as a useful test for evaluation of unilateral renal artery stenoses of moderate de...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Maini A,Gambhir S,Singhal M,Kher V

    更新日期:2000-04-01 00:00:00

  • Kinetic analysis of 99mTc-sestamibi evaluates the protective effects by ischaemic preconditioning on ischaemic myocardium in an isolated rabbit heart.

    abstract:OBJECTIVE:To analyse the kinetic changes of uptake, washout and retention of Tc-sestamibi in order to evaluate the protective effects and possible mechanism of ischaemic preconditioning and adenosine preconditioning on myocardium injured by ischaemia/reperfusion. METHODS:Isolated ischaemia/reperfusion rabbit heart mod...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Liang J,Chen HL,Zhou Y,Xie M,Xu LM,Lin G

    更新日期:2007-11-01 00:00:00

  • 111In-labelled platelets for assessment of thrombogenicity of new haemodialysis vascular access.

    abstract::Using platelet scintigraphy to evaluate early thrombogenicity, we examined 39 new vascular accesses 4 weeks and 3 months after surgery. We found a significant association between platelet deposition and Doppler flow (P < 0.01) and blood pressure (P < 0.01). Compared with arteriovenous fistulae, prosthetic grafts showe...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Neumann I,Meisl FT,Kopriva G,Manker W,Sinzinger H

    更新日期:1999-05-01 00:00:00

  • Preoperative 18F-FDG PET/computed tomography predicts survival following resection for colorectal liver metastases.

    abstract:OBJECTIVE:The liver is the most frequent metastatic site from colorectal cancer and about 20% of these patients are treated by surgical resection. However, the 5-year disease-free survival (DFS) following resection is only about 25% and 5-year overall survival (OS) about 38%. The aim of the study was to evaluate the ab...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Grut H,Stern NM,Dueland S,Labori KJ,Dormagen JB,Schulz A

    更新日期:2020-09-01 00:00:00

  • A retrospective study comparing contrast-enhanced computed tomography with 18F-FDG-PET/CT in the early follow-up of patients with retroperitoneal sarcomas.

    abstract:OBJECTIVE:To compare 18-fluorine-labeled 2-deoxy-2-fluoro-D-glucose PET/computed tomography ((18)F-FDG-PET/CT) with contrast enhancement computed tomography (CECT) in the early follow-up of patients who had undergone treatment for primitive retroperitoneal sarcomas (RS). METHODS:This is a retrospective study on 24 pat...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Niccoli-Asabella A,Altini C,Notaristefano A,Merenda N,Altieri ML,Stabile-Ianora AA,Fanelli M,Rubini G

    更新日期:2013-01-01 00:00:00

  • 99mTc-sestamibi imaging and bone marrow karyotyping in the assessment of multiple myeloma and MGUS.

    abstract:AIM:The first pathogenetic step in multiple myeloma is the emergence of a limited number of clonal plasma cells, clinically known as monoclonal gammopathy of undetermined significance (MGUS). Patients with MGUS do not have symptoms or end-organ damage but they do have a 1% annual risk of progression to multiple myeloma...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Giovanella L,Taborelli M,Ceriani L,Zucca E,Cavalli F,Delaloye AB

    更新日期:2008-06-01 00:00:00

  • Non-invasive quantification of cerebral blood flow for rats by microPET imaging of 15O labelled water: the application of a cardiac time-activity curve for the tracer arterial input function.

    abstract:OBJECTIVE:In-vivo quantitative cerebral blood flow (CBF) measurement using positron emission tomography (PET) has typically employed invasive arterial blood sampling procedure to determine the arterial input function (AIF). The present study was performed to provide a non-invasive quantitative CBF measurement technique...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Yee SH,Jerabek PA,Fox PT

    更新日期:2005-10-01 00:00:00

  • Changes in septal regional ejection fraction early and late following coronary artery bypass grafting in patients without postoperative myocardial infarction.

    abstract::Changes in regional ejection fraction (rEF) of the interventricular septum following coronary artery bypass graft (CABG) surgery were examined using first-pass radionuclide ventriculography (RNVG) in patients without objective evidence of postoperative myocardial infarction (MI). One hundred and one patients had pre- ...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Jacobson AF,Tow DE,Lapsley D,Khuri S

    更新日期:1992-10-01 00:00:00

  • Various imaging modalities for the detection of salivary gland lesions: the advantages of 201Tl SPET.

    abstract::The aim of this study was to compare dual-isotope (99Tc(m) and 201Tl) SPET imaging with computed tomography (CT) and magnetic resonance imaging (MRI) in the differentiation of various lesions of the major salivary glands. Twenty-two patients underwent dual-isotope SPET imaging, of whom 12 also had CT and 15 also had a...

    journal_title:Nuclear medicine communications

    pub_type: 临床试验,杂志文章


    authors: Arbab AS,Koizumi K,Toyama K,Nambu A,Ichikawa T,Arai T,Araki T

    更新日期:2000-03-01 00:00:00

  • Phase I/II clinical trial of the humanized anti-EGF-r monoclonal antibody h-R3 labelled with 99mTc in patients with tumour of epithelial origin.

    abstract:AIM:To evaluate the biodistribution, internal radiation dosimetry and toxicity of the humanized MAb h-R3 labelled with Tc in humans. METHODS:Twenty-five patients with suspected epithelial-derived tumours were included in this study and divided into two groups: group I consisted of 10 patients who received 3 mg/1110 MB...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Torres LA,Perera A,Batista JF,Hernández A,Crombet T,Ramos M,Neninger E,Pérez M,Sánchez EL,Romero S,Aguilar V,Coca MA,Iznaga-Escobar N

    更新日期:2005-12-01 00:00:00

  • Improvement of the amplitude image through statistical filtering.

    abstract::In order to perform automatically the thresholding of the amplitude image obtained by phase analysis, a filter based on the probability density of the amplitude is proposed. It generates the image of the significant amplitude by setting to zero the amplitude if its square is lower than 4a0 log(1/alpha)/N where a0 is t...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Liehn JC,Valeyre J

    更新日期:1987-10-01 00:00:00

  • 131 I-cG250 monoclonal antibody immunoscintigraphy versus [18 F]FDG-PET imaging in patients with metastatic renal cell carcinoma: a comparative study.

    abstract::The aims of this study were to establish the percentage of metastatic renal cell carcinoma (RCC) lesions detected by radioimmunoscintigraphy (RIS) with the chimeric monoclonal antibody 131I-cG250 versus positron emission tomography (PET) with 18F-labelled deoxyglucose ([18F]FDG), and to evaluate the use of these radio...

    journal_title:Nuclear medicine communications

    pub_type: 临床试验,杂志文章


    authors: Brouwers AH,Dorr U,Lang O,Boerman OC,Oyen WJ,Steffens MG,Oosterwijk E,Mergenthaler HG,Bihl H,Corstens FH

    更新日期:2002-03-01 00:00:00

  • 201Tl scintigraphy in the staging of lung cancer, breast cancer and lymphoma.

    abstract::To evaluate 201Tl in the detection of the primary tumour, lymph node involvement and mediastinal spread we have studied a total of 188 patients with histologically proven lung cancer, breast cancer or malignant lymphoma. Ten patients with benign lung disease were also examined. Static images were performed 20 min afte...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Sehweil AM,McKillop JH,Milroy R,Sayed MA,Ziada G,Banham SW,Davidson KG,Ragib A,Omar YT,Abdel-Dayem HM

    更新日期:1990-04-01 00:00:00

  • The role of fluorine-18 fluorodeoxyglucose PET in prognosis evaluation for stem cell transplantation of lymphoma: a systematic review and meta-analysis.

    abstract::The role of fluorine-18 fluorodeoxyglucose PET (F-FDG PET) in prognostic evaluation of pre-stem cell transplantation (SCT) and post-SCT is still uncertain. A systematic review and meta-analysis were carried out to detect the prognostic power of F-FDG PET. 'PubMed', EMBASE, and Springer were searched for relevant artic...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章,meta分析,评审


    authors: Wang C,Li P,Wu S,Lu J,Liu Q,Luo H,Song S

    更新日期:2016-04-01 00:00:00

  • The use of single photon emission computed tomography in depressive disorders.

    abstract::Single photon emission computed tomography (SPECT) and positron emission tomography (PET) have advanced our understanding of the biological underpinnings of depression. There is, however, considerable variability in the literature. Depression is a complex disorder with marked heterogeneity in diagnosis and treatment. ...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章,评审


    authors: Smith DJ,Cavanagh JT

    更新日期:2005-03-01 00:00:00

  • Scintigraphic study of malignant melanoma with 123I-iodoamphetamine.

    abstract::N-isopropyl-p-I-123-iodoamphetamine (123I-IMP) is extracted by the brain in proportion to regional blood flow. It is particularly useful in the clinical assessment of various pathological conditions related to altered cerebral flow. Moreover, it has been reported that 123I-IMP is avidly incorporated into melanin and m...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Watanabe N,Seto H,Yokoyama K,Matsuda H,Shimizu M,Tonami N,Hisada K,Kakishita M

    更新日期:1996-02-01 00:00:00

  • Effect of an additional atropine injection during dobutamine infusion for myocardial SPET.

    abstract::The aim of this study was to examine the value of an additional atropine injection in patients who do not achieve an adequate heart rate during dobutamine infusion for myocardial perfusion SPET (single photon emission tomography). Patients undergoing dobutamine myocardial SPET who failed to achieve > or = 85% of their...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Caner B,Karanfil A,Uysal U,Tokgozoglu L,Aksoyek S,Ugur O,Ciftci I,Atalar E,Kes S,Bekdik C

    更新日期:1997-06-01 00:00:00

  • Performance of a single crystal digital gamma camera for first pass cardiac studies.

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    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Dobbeleir A,Franken PR,Ham HR,Brihaye C,Guillaume M,Knapp FF,Vandevivere J

    更新日期:1991-01-01 00:00:00

  • National survey of imaging technologists in nuclear medicine 1998/99.

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    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Sanders L,Houston AS

    更新日期:2001-05-01 00:00:00

  • Z-transform method for deconvolution as applied to the renogram.

    abstract::A method of deconvolution analysis involving the use of the z-transform has been developed. When compared to the matrix method, it is shown, under certain circumstances, to be identical. In order to study the validity of the method, simple mathematical models have been constructed. The curves obtained with the compute...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Bererhi H

    更新日期:1995-03-01 00:00:00

  • Correlation of 99mTc-sestamibi uptake with blood-pool and osseous phase 99mTc-MDP uptake in malignant bone and soft-tissue tumours.

    abstract::Technetium-99m-sestamibi (99mTc-MIBI) imaging is a well-established modality in oncologic investigations. The current study aimed to investigate whether any relationship could be found between 99mTc-MIBI uptake and local perfusion in malignant bone and soft-tissue tumours. It also aimed to compare 99mTc-MIBI images wi...

    journal_title:Nuclear medicine communications

    pub_type: 临床试验,杂志文章


    authors: Ozcan Z,Burak Z,Erinç R,Dirlik A,Başdemir G,Sabah D,Ozkiliç H

    更新日期:2001-06-01 00:00:00

  • Diagnostic value of PET/CT is similar to that of conventional MRI and even better for detecting small peritoneal implants in patients with recurrent ovarian cancer.

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    journal_title:Nuclear medicine communications

    pub_type: 杂志文章


    authors: Sanli Y,Turkmen C,Bakir B,Iyibozkurt C,Ozel S,Has D,Yilmaz E,Topuz S,Yavuz E,Unal SN,Mudun A

    更新日期:2012-05-01 00:00:00