Abstract：
:Cationic liposomes are commonly used to introduce foreign genes to the target cells. However, the methods are not applicable to several types of cells such as neuronal cells. We introduced the luciferase gene to the neuronal pheochromocytoma-12 (PC12) cells by cationic liposomes with a cationic cholesterol derivative (I) or 3- beta - [N - (N',N' - dimethylaminoethane) - carbamoyl]- cholesterol (DC - Chol). Efficiency of gene transfection into undifferentiated naive PC12 cells was extremely higher by the liposomes with the derivative (I) than that by the liposomes with DC - Chol. In addition, when the luciferase gene was transferred into the naive PC12 cells in the presence of nerve growth factor (NGF), the luciferase activity increased much higher than that in the absence of NGF. The transfection efficiency was also promoted by epidermal growth factor in the naive PC12 cells. This high efficiency was maintained in the differentiated PC12 cells obtained by incubating the cells with NGF for 7 days. We found that the transfection efficiency did not depend mainly on the differentiated states of PC12 cells but depended on the activation of receptor tyrosine kinases. It is supposed that the novel liposomes with the derivative (I) will be useful to study the problems in neuron and neuronal tissues for gene transfection.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Noguchi A,Hirashima N,Furuno T,Nakanishi Mdoi
10.1016/s0304-3940(02)00238-0keywords:
subject
Has Abstractpub_date
2002-05-31 00:00:00pages
29-32issue
1eissn
0304-3940issn
1872-7972pii
S0304394002002380journal_volume
325pub_type
杂志文章abstract：:Representation of peripheral branches of the facial nerve within the facial nucleus of the cat was examined by utilizing retrograde axonal transport of horseradish peroxidase (HRP), which was injected into groups of muscles supplied by each of the main peripheral branches of the facial nerve. The cervical branch was r...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(78)90088-5
更新日期：1978-04-01 00:00:00
abstract：:High K+ (50 mM) increased both [3H]acetylcholine ([3H]ACh) release and cytosolic free calcium level ([Ca2+]i) in rat brain synaptosomes in the presence of extracellular Ca2+. Ouabain (5 x 10(-8) to 5 x 10(-4) M) also caused a dose-dependent increase in [3H]ACh release, but not in [Ca2+]i, in the absence of Ca2+. The e...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90832-x
更新日期：1989-12-15 00:00:00
abstract：:Small-fiber neuropathy was induced in young adult mice by intraperitoneal injection of resiniferatoxin (RTX), a TRPV1 agonist. At day 7, RTX induced significant thermal and mechanical hypoalgesia. At day 28, mechanical and thermal nociception were restored. No nerve degeneration in skin was observed and unmyelinated n...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2014.04.026
更新日期：2014-06-13 00:00:00
abstract：:To clarify the possible involvement of protein kinase (PK) C activation in the excitation and sensitization of polymodal receptors (PMRs), the effects of phorbol 12,13-dibutyrate (PDBu) on PMRs were studied in canine testis-spermatic nerve preparations in vitro. Application of PDBu (10(-7), 10(-6), AND 10(-5) M) for 5...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(96)12414-9
更新日期：1996-03-08 00:00:00
abstract：:The sexual differences and the seasonal variation in the vasoactive intestinal peptide (VIP) content of the suprachiasmatic nucleus (SCN) of a desert rodent, the jerboa (Jaculus orientalis) were studied using immunocytochemical techniques. During the period of sexual activity (spring-early summer), the VIP immunoreact...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(92)90708-f
更新日期：1992-09-14 00:00:00
abstract：:Intellectual disability (ID) is one of the most prevalent chronic developmental brain disorders or phenotype of syndromic ID, affecting nearly 1-2% of the general population worldwide. Over recent decades, tremendous effort and high-throughput platforms have been devised to explore the complex heterogeneity, numerous ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2018.08.027
更新日期：2018-10-15 00:00:00
abstract：:Neuroglobin (Ngb) is a novel vertebrate globin expressed principally in neurons. Ngb expression is induced by hypoxia and ischemia, and Ngb protects neurons against these insults. The mechanism of Ngb's protective action is unknown, but its ability to bind NO suggests that NO scavenging might be involved. To test this...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2007.10.031
更新日期：2008-01-10 00:00:00
abstract：:The synaptic relationships between gamma-aminobutyric acid (GABA)-immunoreactive and enkephalin-immunoreactive profiles in the cat spinal cord were examined using combined pre-embedding immunoperoxidase and post-embedding immunogold electron microscopic immunocytochemistry. Although colchicine was not used, enkephalin...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(92)90501-w
更新日期：1992-04-13 00:00:00
abstract：:Highly purified and metabolically viable gamma-aminobutyric acid (GABA)ergic and cholinergic synaptosomes were prepared from mammalian brain using antisera raised against glutamate decarboxylase and choline acetyltransferase respectively. These antisera appear to recognise specific outwardly facing components of GABAe...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(87)91004-4
更新日期：1987-10-16 00:00:00
abstract：:The homozygous Brattleboro rat (di/di) synthesizes a vasopressin (VP) precursor with an aberrant C-terminus, which causes a hypothalamic form of diabetes insipidus. The neuroendocrine polypeptide 7B2 is present in VP and oxytocin (OT) neurons of the supraoptic and paraventricular nucleus of the hypothalamus in wild ty...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(97)00883-5
更新日期：1997-12-12 00:00:00
abstract：:For several neurodegenerative disorders, including Parkinson's Disease (PD) and Alzheimer's Disease (AD), microRNAs (miRNAs) have been known to play a crucial role. So, in this study miR-132 and its role in PD cell models was investigated. We wanted to investigate the survival or death pathway involved in PD. We obser...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2020.135465
更新日期：2021-01-01 00:00:00
abstract：:The potential neurotoxicity of the solvent toluene to the nigrostriatal dopaminergic system was assessed in a rat model of Parkinson's disease. Rats, 1 day after a unilateral injection of 6-hydroxydopamine (6-OH DA) into the substantia nigra, inhaled air or different concentrations of toluene (80, 300 or 1000 ppm), 6 ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(96)13049-4
更新日期：1996-10-11 00:00:00
abstract：:Stereotaxic microinjection of herpes simplex virus (HSV) into the mouse olfactory bulb resulted in infection of neurons of the piriform cortex. Neurons infected with the wildtype HSV showed no evident phosphorylation of c-Jun N-terminal protein kinase (JNK)/c-Jun. In contrast, neurons infected with a US3 gene-disrupte...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2003.08.033
更新日期：2003-11-20 00:00:00
abstract：:A partially purified toxin obtained from the coral reef crab, Lophozozymus pictor was found to be a potent inhibitor of Na+-dependent [14C]-GABA uptake by rat brain synaptosomes. Inhibition was potentiated in the presence of external K+. The I50 value for the toxin was found to be in the region of 10 microgram/ml at a...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(79)96171-8
更新日期：1979-10-01 00:00:00
abstract：:Implicit detection of statistical regularities is thought to be a ubiquitous facet of cognition; yet, we know little about statistical learning (SL) over time. A recent study showed that visual SL can be observed at 24 h post stimulus (Kim et al., 2009 [14]). Here we sought to obtain a finer-grained picture of visual ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2012.04.045
更新日期：2012-05-31 00:00:00
abstract：:In order to assess functional skills of older adults, both timed up and go (TUG) test and gait speed (GS) test are well validated concerning their predictive capacities. However, the question remains unclear which one of these tests represents better the whole physical performance of older adults. The aim of this stud...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2014.06.014
更新日期：2014-08-08 00:00:00
abstract：:The paraoxonase (PON1) Gln192-->Arg polymorphism was examined in a group of sporadic late-onset Alzheimer's disease (AD) patients, in a group of coronary artery disease (CAD) patients, and in normal subjects. The AD sample showed a PON1*R allele frequency significantly lower than the control group (0.225 vs. 0.281, P=...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(02)01437-4
更新日期：2003-03-13 00:00:00
abstract：:The sigma-1 (σ1) receptor is a chaperone protein located on the mitochondria-associated membrane of the endoplasmic reticulum, while the sigma-2 receptor (σ2) is an endoplasmic reticulum-resident membrane protein. Recent evidence indicates that both of these receptors figure prominently in the pathophysiology of Alzhe...
journal_title：Neuroscience letters
pub_type： 杂志文章,评审
doi：10.1016/j.neulet.2018.07.033
更新日期：2019-01-19 00:00:00
abstract：:To assess cortical activity during pain perception, regional cerebral blood flow (rCBF) studies were done in humans using single photon emission computed tomography (SPECT) with the radiotracer Tc99m-HMPAO and magnetic resonance imaging localization. Normalized SPECT data were analyzed by region of interest and change...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(92)90088-o
更新日期：1992-06-22 00:00:00
abstract：:Previous studies on the effects of serotonin receptor 1A (5-HT1A) gene variation on treatment response in depression revealed inconsistent results with studies pointing towards a detrimental influence of the 5-HT1A-1019G allele on antidepressant treatment response, while others did not discern any involvement of 5-HT1...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.03.001
更新日期：2008-05-09 00:00:00
abstract：:The brain and nasal region of a fetal (124 days) female rhesus macaque (Macaca mulatta) were fixed in situ, decalcified, sectioned and treated for the immunocytochemical demonstration of LHRH. Immunoreactive neurons and fibers were found in expected sites and also in sites not reported to date in primates: there was a...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(87)90674-4
更新日期：1987-08-18 00:00:00
abstract：:gamma-Aminobutyric acid-transaminase (GABA-T) plays an important role in the metabolism of GABA, particularly in the neurons or glial cells. The present study was undertaken to determine the alteration of GABA-T expression in the gerbil hippocampus after ischemia-reperfusion. In the sham, GABA-T(+) neurons were scatte...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)01541-x
更新日期：2000-11-10 00:00:00
abstract：:This study examined the effects of exposure to low concentrations (0.1-100 nM) of bisphenol A (BPA) or nonylphenol (NP) on neuronal differentiation in pheochromocytoma PC12 cells. Pre-exposure to BPA for a week or a month, but not for a day, decreased neuronal differentiation in PC12 cells. Likewise, one week's pre-ex...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2014.04.014
更新日期：2014-06-06 00:00:00
abstract：:The effect of chlorpromazine (CPZ) on deactivation of N-methyl-D-aspartate (NMDA)-induced currents was studied in the whole-cell configuration of the patch-clamp technique in cultured rat hippocampal neurons. We report that CPZ (at 30-1000 microM) strongly slowed down the deactivation process in a dose-dependent manne...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(01)02245-5
更新日期：2001-11-23 00:00:00
abstract：:Paramethoxyphenylethylamine (PMPEA) is a sympathomimetic amine, like its parent compound, phenylethylamine. The intraperitoneal administration of PMPEA to mice produces a dose-dependent depletion of brain and heart norepinephrine, with significant reduction being observed one hour postinjection. At lower dosages, PMPE...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(75)90033-6
更新日期：1975-09-01 00:00:00
abstract：:Myenteric neurons which innervate the mucosa of the guinea-pig ileum were characterized by combining retrograde transport of DiI in vitro with immunohistochemistry. Of DiI-labelled myenteric neurons, 43% were immunoreactive for calbindin and substance P, 25% were immunoreactive for calbindin alone, and 18% were immuno...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(91)90484-b
更新日期：1991-08-19 00:00:00
abstract：:Adenosine triphosphate (ATP) is well known to be released from injured or inflamed tissues, and to excite/sensitize nociceptors in response to heat and mechanical stimulation. To determine whether muscle releases ATP when it is compressed, we measured ATP release from the extensor digitorum longus muscle (EDL). In add...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.01.068
更新日期：2008-04-04 00:00:00
abstract：BACKGROUND AND PURPOSE:The pathophysiology of cerebral aneurysms (CA) is linked to chronic inflammation. Endothelial damage is one of the first changes in CA walls resulted from inflammation. It has been shown that increase in plasma homocysteine (Hcy) impairs vascular endothelium and correlates with the development of...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2011.02.076
更新日期：2011-04-25 00:00:00
abstract：:In neuro-inflammatory diseases, activated T cells are thought to drive the inflammatory process. In this study, we investigated the potential role of three T cell attracting chemokines (CK) in neuro-inflammation. For this purpose, we measured levels of CXCL16, CCL17 and CCL18 in matched serum and cerebrospinal fluid (...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.12.029
更新日期：2006-04-10 00:00:00
abstract：:Traumatic spinal cord injury (SCI) is a devastating condition of CNS which leads to loss of sensory as well as motor functions. Secondary damage after SCI initiates cascade of events that creates an inhibitory milieu for axonal growth and repair. Combinatorial therapies are the hope to attenuate secondary injury progr...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2020.135500
更新日期：2021-01-10 00:00:00
