Activation of receptor tyrosine kinases promotes gene transfection in rat neuronal PC12 cells by cationic liposomes.


:Cationic liposomes are commonly used to introduce foreign genes to the target cells. However, the methods are not applicable to several types of cells such as neuronal cells. We introduced the luciferase gene to the neuronal pheochromocytoma-12 (PC12) cells by cationic liposomes with a cationic cholesterol derivative (I) or 3- beta - [N - (N',N' - dimethylaminoethane) - carbamoyl]- cholesterol (DC - Chol). Efficiency of gene transfection into undifferentiated naive PC12 cells was extremely higher by the liposomes with the derivative (I) than that by the liposomes with DC - Chol. In addition, when the luciferase gene was transferred into the naive PC12 cells in the presence of nerve growth factor (NGF), the luciferase activity increased much higher than that in the absence of NGF. The transfection efficiency was also promoted by epidermal growth factor in the naive PC12 cells. This high efficiency was maintained in the differentiated PC12 cells obtained by incubating the cells with NGF for 7 days. We found that the transfection efficiency did not depend mainly on the differentiated states of PC12 cells but depended on the activation of receptor tyrosine kinases. It is supposed that the novel liposomes with the derivative (I) will be useful to study the problems in neuron and neuronal tissues for gene transfection.


Neurosci Lett


Neuroscience letters


Noguchi A,Hirashima N,Furuno T,Nakanishi M





Has Abstract


2002-05-31 00:00:00














  • Amflutizole, a xanthine oxidase inhibitor, inhibits free radical generation in the ischemic/reperfused rat cerebral cortex.

    abstract::Free radical generation and release from the cerebral hemispheres of rats subjected to four-vessel occlusion elicited cerebral ischemia/reperfusion was monitored using a cortical cup technique with the spin-trapping agent alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN). Electron spin resonance (ESR) was used to d...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Phillis JW,Sen S,Cao X

    更新日期:1994-03-14 00:00:00

  • D-cycloserine enhances both intrinsic excitability of CA1 hippocampal neurons and expression of activity-regulated cytoskeletal (Arc) protein.

    abstract::The interaction of NMDA receptor (NMDAR) activation and other mechanisms regulating neuronal excitability have not been thoroughly described. While excess activation of NMDARs results in excitotoxicity, partial activation of NMDARs by d-cycloserine (DCS) is nootropic, enhancing both acquisition and extinction of memor...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Donzis EJ,Thompson LT

    更新日期:2014-06-13 00:00:00

  • Biochemical, neuropathological, and neuroimaging characteristics of early-onset Alzheimer's disease due to a novel PSEN1 mutation.

    abstract::Familial Alzheimer's disease (AD) due to PSEN1 mutations provides an opportunity to examine AD biomarkers in persons in whom the diagnosis is certain. We describe a 55 year-old woman with clinically probable AD and a novel PSEN1 mutation who underwent genetic, clinical, biochemical and magnetic resonance and nuclear i...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Ringman JM,Gylys KH,Medina LD,Fox M,Kepe V,Flores DL,Apostolova LG,Barrio JR,Small G,Silverman DH,Siu E,Cederbaum S,Hecimovic S,Malnar M,Chakraverty S,Goate AM,Bird TD,Leverenz JB

    更新日期:2011-01-10 00:00:00

  • Age and binding within-dimension features in visual short-term memory.

    abstract::Older adults have difficulties in binding information in long-term memory (e.g. objects with colours). The effect of age on visual short-term memory (VSTM) binding is less well understood. Recent evidence has suggested that older adults' VSTM for colours bound to shapes or for locations bound in configural representat...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Parra MA,Abrahams S,Logie RH,Sala SD

    更新日期:2009-01-02 00:00:00

  • Excitation and sensitization of the heat response induced by a phorbol ester in canine visceral polymodal receptors studied in vitro.

    abstract::To clarify the possible involvement of protein kinase (PK) C activation in the excitation and sensitization of polymodal receptors (PMRs), the effects of phorbol 12,13-dibutyrate (PDBu) on PMRs were studied in canine testis-spermatic nerve preparations in vitro. Application of PDBu (10(-7), 10(-6), AND 10(-5) M) for 5...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Leng S,Mizumura K,Koda H,Kumazawa T

    更新日期:1996-03-08 00:00:00

  • Cerebellar corticovestibular projections from lobule IX to the descending vestibular nucleus in the cat. A retrograde wheat germ agglutinin-horseradish peroxidase study.

    abstract::The distribution of Purkinje cells projecting to the descending vestibular nucleus was studied in lobule IX (uvula) of the cat by the retrograde wheat germ agglutinin-horseradish peroxidase method. In the transverse plane labeled Purkinje cells were seen diffusely within 1.0 mm from the midline and densely for 250-500...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Matsushita M,Wang CL

    更新日期:1986-05-23 00:00:00

  • DJ-1 mRNA anatomical localization and cell type identification in the mouse brain.

    abstract::Mutations in DJ-1 cause familial Parkinson's disease (PD). The expression pattern of DJ-1 in the brain remains controversial. In the present study, we used DJ-1 deficient mice as negative controls and examined DJ-1 mRNA expression in mouse brains. In sequential double labeling on the same sections, in situ hybridizati...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Xie Z,Zhuang X,Chen L

    更新日期:2009-11-20 00:00:00

  • PIN1-842G/C and -667T/C polymorphisms are not associated with the susceptibility of Alzheimer's disease: pooled analysis of epidemiologic studies.

    abstract::Associations between PIN1 gene polymorphisms and Alzheimer's disease (AD) risk remain controversial, possibly because single studies often lack sufficient statistical power. In this study, we performed a meta-analysis to evaluate the association of two commonly studied PIN1 polymorphisms, -842G/C and -667T/C, with the...

    journal_title:Neuroscience letters

    pub_type: 杂志文章,meta分析


    authors: Cao L,Wang F,Ge H,Wu PC,Qu P,Chen GH,Gao ZL

    更新日期:2013-02-22 00:00:00

  • Intranasal administration of NAP, a neuroprotective peptide, decreases anxiety-like behavior in aging mice in the elevated plus maze.

    abstract::NAP, an 8-amino-acid peptide (NAPVSIPQ=Asn-Ala-Pro-Val-Ser-Ile-Pro-Gln), provides neuroprotection at very low doses in a variety of animal models. Previously, acute NAP administration by the intranasal route resulted in improved performance in the Morris water maze of normal and cognitively impaired rats. In these ani...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Alcalay RN,Giladi E,Pick CG,Gozes I

    更新日期:2004-05-06 00:00:00

  • Fast axonal transport in rat sciatic nerve. Inhibition by pineal indoles.

    abstract::The effect of pineal indoles on fast axoplasmic transport of proteins in the sciatic nerve was examined in rats injected with [3H]leucine in the sixth lumbar dorsal root ganglion. Melatonin (350-1100 nmol) applied locally in the sciatic nerve impaired significantly axonal transport. At a 350-nmol dose all other indole...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Prevedello MR,Ritta MN,Cardinali DP

    更新日期:1979-06-01 00:00:00

  • Acupuncture at SI5 attenuates morphine seeking behavior after extinction.

    abstract::Our previous studies have shown that acupuncture attenuates morphine self-administration and sensitization behavior as well as withdrawal signs. The present study was designed to investigate the role of acupuncture in the reinstatement of morphine seeking. Male Sprague-Dawley rats weighing 270-300 g were subjected to ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Lee BH,Ma JH,In S,Kim HY,Yoon SS,Jang EY,Yang CH

    更新日期:2012-10-31 00:00:00

  • Neurite outgrowth of murine cerebellar granule cells can be enhanced by aniracetam with or without alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA).

    abstract::To assess the neurotrophic effects of a nootropic drug, aniracetam, we studied neurite extension of mouse cerebellar granule cells in culture with low or with high K+ under different combinations of drugs and then immunohistochemically stained the cells with an antibody against L1, a neural cell adhesion molecule on c...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Fushiki S,Matsumoto K,Nagata A

    更新日期:1995-10-27 00:00:00

  • Induction of memory-associated immediate early genes by nerve growth factor in rat primary cortical neurons and differentiated mouse Neuro2A cells.

    abstract::Activation of several immediate early genes (IEGs) is crucial for long-term memory formation in vivo. In vitro methods of inducing these genes have not been investigated extensively. Here we present data demonstrating that application of the neurotrophin, nerve growth factor (NGF), to both rat primary neuronal culture...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Dickey CA,De Mesquita DD,Morgan D,Pennypacker KR

    更新日期:2004-08-05 00:00:00

  • Mismatch negativity (MMN) to speech sounds is modulated systematically by manual grip execution.

    abstract::Manual actions and speech are connected: for example, grip execution can influence simultaneous vocalizations and vice versa. Our previous studies show that the consonant [k] is associated with the power grip and the consonant [t] with the precision grip. Here we studied whether the interaction between speech sounds a...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Tiainen M,Tiippana K,Paavilainen P,Vainio M,Vainio L

    更新日期:2017-06-09 00:00:00

  • Oxidative stress and overexpression of manganese superoxide dismutase in patients with Alzheimer's disease.

    abstract::Substantial evidence supports the hypothesis that oxygen free radicals are involved in various neurodegenerative disorders. To assess the presence of oxidative stress in Alzheimer's disease (AD) we examined the activity of the enzyme copper-zinc superoxide dismutase (CuZnSOD) in red blood cells, the levels of the mito...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: De Leo ME,Borrello S,Passantino M,Palazzotti B,Mordente A,Daniele A,Filippini V,Galeotti T,Masullo C

    更新日期:1998-07-10 00:00:00

  • Heroin-based crack induces hyperalgesia through β-arrestin 2 redistribution and phosphorylation of Erk1/2 and JNK in the periaqueductal gray area.

    abstract::Continuous use of crack induces hyperalgesia which is related to drug tolerance. Despite cumulative evidence based on the growth rate of crack abuse, no serious study has been focused on the mechanisms of crack-induced hyperalgesia. This study aimed to elucidate whether extracellular signal-regulated kinases (Erk1/2)/...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Aberoumandi SM,Vousooghi N,Tabrizi BA,Karimi P

    更新日期:2019-04-17 00:00:00

  • The basal nucleus of Meynert in patients with progressive supranuclear palsy.

    abstract::The neuronal number and nucleolar volume of the magnocellular population of the basal nucleus of Meynert in 6 patients with progressive supranuclear palsy (PSP) - one associated with Alzheimer's disease - were compared with those of 5 controls. In the 5 typical PSP cases, an age-dependent neuronal loss was demonstrate...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Tagliavini F,Pilleri G,Bouras C,Constantinidis J

    更新日期:1984-01-27 00:00:00

  • Localization of dopamine receptor subtypes in the rat spiral ganglion.

    abstract::Although dopaminergic neurons are thought to exist in the lateral olivocochlear efferent system and modulate the afferent nerve activity, the distribution of dopamine (DA) receptor subtypes is still obscure. In the present study, we investigated the localization of five subtypes of DA receptor (D1-5) by immunocytochem...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Inoue T,Matsubara A,Maruya S,Yamamoto Y,Namba A,Sasaki A,Shinkawa H

    更新日期:2006-05-22 00:00:00

  • Microtubular packing varies along the course of motor and sensory axons: possible regulation of microtubules by environmental cues.

    abstract::In the toad Xenopus laevis, the microtubular density of 3-microns myelinated fibres was assessed in peripheral nerves, dorsal and ventral roots, and dorsal and ventral funiculi of the spinal cord. In the roots, the axonal microtubular density was 6 microtubules/microns 2 and twice as much at the other sampling sites. ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Saitua F,Alvarez J

    更新日期:1989-10-09 00:00:00

  • Interleukin-12 promotes neurite outgrowth in mouse sympathetic superior cervical ganglion neurons.

    abstract::To determine the role of cytokines in the nervous system, we examined the effect of interleukin-12(IL-12) on the nerve regeneration of mouse superior cervical ganglion cells (SCG). IL-12 enhanced the neurite outgrowth in a concentration-dependent manner. Immunocytochemical studies demonstrated the expression of IL-12 ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Lin H,Hikawa N,Takenaka T,Ishikawa Y

    更新日期:2000-01-14 00:00:00

  • Immortalization of a cell line showing some characteristics of the oligodendrocyte phenotype.

    abstract::We have used the polyoma middle T oncogene to immortalize cells from rat embryo encephalon. Immunostaining experiments with monoclonal antibodies demonstrated that the cells of one of the obtained lines, named CEINGE CL3, are stained by anti-vimentin and anti-S100 antibodies, are not stained by anti-neurofilaments (NF...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Russo T,Mogavero AR,Ammendola R,Mesuraca M,Fiore F,Fatatis A,Salvatore G,Cimino F

    更新日期:1993-09-03 00:00:00

  • Rutin alleviates chronic unpredictable stress-induced behavioral alterations and hippocampal damage in mice.

    abstract::Chronic stress results in neurological complications like depression, cognitive dysfunction, and anxiety disorders. In our previous study, we observed that Urtica dioica leaf extract attenuated chronic stress-induced complications. Further, we observed that Urtica dioica contained a great amount of the flavonoid rutin...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Parashar A,Mehta V,Udayabanu M

    更新日期:2017-08-24 00:00:00

  • Identification of astrocyte- and oligodendrocyte-like cells of goldfish optic nerves in culture.

    abstract::Fish glial cells were obtained from cultivated segments of the optic nerve and raised in vitro. Two types of cells were identified as astrocyte- and oligodendrocyte-like glia by the monoclonal antibody Mab O1 (specific for oligodendrocytes) and the rabbit serum anti-goldfish glial fibrillary acidic protein (anti-G-GFA...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Bastmeyer M,Beckmann M,Nona SM,Cronly-Dillon JR,Stuermer CA

    更新日期:1989-06-19 00:00:00

  • Examining fMRI time-series entropy as a marker for brain E/I balance with pharmacological neuromodulation in a non-human primate translational model.

    abstract::Recently, there has been a lot of interest in the neuroimaging community in exploring fMRI time-series measures of local neuronal activity and excitation/inhibition (E/I) balance in the brain. In this preliminary study we probed the sensitivity of widely used sample entropy (SE) measure at multiple scales to controlle...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Maltbie EA,Howell LH,Sun PZ,Miller R,Gopinath KS

    更新日期:2020-05-29 00:00:00

  • Involvement of peripherally released substance P and calcitonin gene-related peptide in mediating mechanical hyperalgesia in a traumatic neuropathy model of the rat.

    abstract::We hypothesized that neuropeptides released from the peripheral terminals of primary afferents play an important role in mechanical hyperalgesia after peripheral nerve injury. Nerve injury was performed on rats with lumbar 5 spinal nerve lesion (L5 SNL), which was preceded by L5 dorsal rhizotomy (L5 DR) to avoid the p...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Jang JH,Nam TS,Paik KS,Leem JW

    更新日期:2004-04-29 00:00:00

  • The distribution of histidine decarboxylase mRNA in the rat brain: an in situ hybridization study using synthetic oligonucleotide probes.

    abstract::L-Histidine decarboxylase catalyzes the formation of histamine from the amino acid L-histidine. We have studied the distribution of neurons expressing mRNA for histidine decarboxylase in adult rat brain using in situ hybridization with synthetic oligonucleotide probes. The expression of mRNA for histidine decarboxylas...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Castrén E,Panula P

    更新日期:1990-11-27 00:00:00

  • The ultrastructural localisation of the N-methyl-D-aspartate NR2B receptor subunit in rat lumbar spinal cord.

    abstract::Glutamate together with its N-methyl-d-aspartate (NMDA) receptors has an important role in the transmission of stimuli in the spinal cord. Whilst the expression of the various NMDA receptor subunits within the spinal cord has been investigated the subcellular location of the NMDA NR2B subunit has yet to be definitivel...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: O'Donnell R,Molon-Noblot S,Laroque P,Rigby M,Smith D

    更新日期:2004-11-16 00:00:00

  • Calyculin A blocks bovine chromaffin cell calcium channels independently of phosphatase inhibition.

    abstract::The specific phosphatase inhibitor, Calyculin-A (CL-A), decreases high-K stimulated catecholamine secretion in bovine chromaffin cells. This effect can be split into two components: one needs long exposures to the drug to be elicited, and is sensitive to the protein kinase-inhibitor K252a; the other is observed after ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Gutiérrez LM,Viniegra S,Quintanar JL,Reig JA,Sala F

    更新日期:1994-08-29 00:00:00

  • Lithium increases plasma brain-derived neurotrophic factor in acute bipolar mania: a preliminary 4-week study.

    abstract::Several studies have suggested an important role for brain-derived neurotrophic factor (BDNF) in the pathophysiology and therapeutics of bipolar disorder (BPD). The mechanisms underlying the therapeutic effects of lithium in BPD seem to involve a direct regulation of neurotrophic cascades. However, no clinical study e...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: de Sousa RT,van de Bilt MT,Diniz BS,Ladeira RB,Portela LV,Souza DO,Forlenza OV,Gattaz WF,Machado-Vieira R

    更新日期:2011-04-20 00:00:00

  • Suppression of postsynaptic density protein 95 expression attenuates increased tyrosine phosphorylation of NR2A subunits of N-methyl-D-aspartate receptors and interactions of Src and Fyn with NR2A after transient brain ischemia in rat hippocampus.

    abstract::The effects of suppression of postsynaptic density protein 95 (PSD-95) expression on the increased tyrosine phosphorylation of N-methyl-D-aspartate receptor subunit NR2A and interactions of Src and Fyn with NR2A after brain ischemia were investigated by immunoprecipitation and immunoblotting. Transient (15 min) brain ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Hou XY,Zhang GY,Zong YY

    更新日期:2003-06-05 00:00:00