Cloning and characterization of a phospholipase C from the C(4) plant Digitaria sanguinalis.


:As a PLC activity was implicated in the light transduction pathway that controls C(4) photosynthesis in Digitaria sanguinalis, a full length PLC cDNA (DsPLC2) was cloned. The proteins encoded by the two possible open reading frames were produced in Escherichia coli; they both harbour a PLC activity but with different response to Ca(2+) concentration, and with different sensitivity to the PLC inhibitor U-73122.


J Exp Bot


Coursol S,Pierre JN,Vidal J,Grisvard J



Has Abstract


2002-06-01 00:00:00












  • Natural variation in stomatal response to closing stimuli among Arabidopsis thaliana accessions after exposure to low VPD as a tool to recognize the mechanism of disturbed stomatal functioning.

    abstract::Stomatal responses to closing stimuli are disturbed after long-term exposure of plants to low vapour pressure deficit (VPD). The mechanism behind this disturbance is not fully understood. Genetic variation between naturally occurring ecotypes can be helpful to elucidate the mechanism controlling stomatal movements in ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Aliniaeifard S,van Meeteren U

    更新日期:2014-12-01 00:00:00

  • Functions of two genes in aluminium (Al) stress resistance: repression of oxidative damage by the AtBCB gene and promotion of efflux of Al ions by the NtGDI1gene.

    abstract::The functions of two genes whose expression provides tolerance to aluminium (Al) stress were investigated using plants and Saccharomyces cerevisiae (yeast): the Arabidopsis thaliana blue copper binding gene (AtBCB) and Nicotiana tabacum guanosine diphosphate (GDP) dissociation inhibitor gene (NtGDI1). To determine the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Ezaki B,Sasaki K,Matsumoto H,Nakashima S

    更新日期:2005-10-01 00:00:00

  • Hydraulic properties of individual xylem vessels of Fraxinus americana.

    abstract::Studies of the hydraulic properties of xylem vessels have been limited to measurements of whole plant or whole stem segments. This approach allows the longitudinal transport properties of the ensemble of vessels within a stem to be determined, but provides little information on radial transport. Here the xylem of Frax...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Zwieniecki MA,Melcher PJ,Holbrook NM

    更新日期:2001-02-01 00:00:00

  • Improving the nutritive value of rice seeds: elevation of cysteine and methionine contents in rice plants by ectopic expression of a bacterial serine acetyltransferase.

    abstract::With the aim of increasing the cysteine level in rice (Oryza sativa L.) and thus improving its nutritional quality, transgenic rice plants were generated expressing an Escherichia coli serine acetyltransferase isoform (EcSAT), the enzyme synthesizing O-acetylserine, the precursor of cysteine. The gene was fused to the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Nguyen HC,Hoefgen R,Hesse H

    更新日期:2012-10-01 00:00:00

  • Giant cacti: isotopic recorders of climate variation in warm deserts of the Americas.

    abstract::The plant family Cactaceae is considered among the most threatened groups of organisms on the planet. The threatened status of the cacti family has created a renewed interest in the highly evolved physiological and morphological traits that underpin their persistence in some of the harshest subtropical environments in...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Hultine KR,Dettman DL,English NB,Williams DG

    更新日期:2019-11-29 00:00:00

  • Early changes in membrane permeability, production of oxidative burst and modification of PAL activity induced by ergosterol in cotyledons of Mimosa pudica.

    abstract::Ergosterol (a fungal membrane component) was shown to induce transient influx of protons and membrane hyperpolarization in cotyledonary cells of Mimosa pudica L. By contrast, chitosan (a fungal wall component with known elicitor properties) triggered membrane depolarization. In the processes induced by ergosterol, a s...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Rossard S,Luini E,Pérault JM,Bonmort J,Roblin G

    更新日期:2006-01-01 00:00:00

  • Three differentially expressed basic peroxidases from wound-lignifying Asparagus officinalis.

    abstract::The activity of ionically bound peroxidases from an asparagus spear increased from 5-24 h post-harvest. Isoelectric focusing showed that the post-harvest increase of the total peroxidase activity was due to the increase of several distinct isoperoxidases. Concomitantly, a decrease in the activity of two anionic peroxi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Holm KB,Andreasen PH,Eckloff RM,Kristensen BK,Rasmussen SK

    更新日期:2003-10-01 00:00:00

  • Gene expression profiles in rice gametes and zygotes: identification of gamete-enriched genes and up- or down-regulated genes in zygotes after fertilization.

    abstract::In angiosperms, fertilization and subsequent zygotic development occur in embryo sacs deeply embedded in the ovaries; therefore, these processes are poorly elucidated. In this study, microarray-based transcriptome analyses were conducted on rice sperm cells, egg cells, and zygotes isolated from flowers to identify can...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Abiko M,Maeda H,Tamura K,Hara-Nishimura I,Okamoto T

    更新日期:2013-04-01 00:00:00

  • NH4+-stimulated and -inhibited components of K+ transport in rice (Oryza sativa L.).

    abstract::The disruption of K(+) transport and accumulation is symptomatic of NH(4)(+) toxicity in plants. In this study, the influence of K(+) supply (0.02-40 mM) and nitrogen source (10 mM NH(4)(+) or NO(3)(-)) on root plasma membrane K(+) fluxes and cytosolic K(+) pools, plant growth, and whole-plant K(+) distribution in the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Szczerba MW,Britto DT,Ali SA,Balkos KD,Kronzucker HJ

    更新日期:2008-01-01 00:00:00

  • A comparative study of ripening among berries of the grape cluster reveals an altered transcriptional programme and enhanced ripening rate in delayed berries.

    abstract::Transcriptional studies in relation to fruit ripening generally aim to identify the transcriptional states associated with physiological ripening stages and the transcriptional changes between stages within the ripening programme. In non-climacteric fruits such as grape, all ripening-related genes involved in this pro...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Gouthu S,O'Neil ST,Di Y,Ansarolia M,Megraw M,Deluc LG

    更新日期:2014-11-01 00:00:00

  • Ca2+-dependent nuclease is involved in DNA degradation during the formation of the secretory cavity by programmed cell death in fruit of Citrus grandis 'Tomentosa'.

    abstract::The secretory cavity is a typical structure in Citrus fruit and is formed by schizolysigeny. Previous reports have indicated that programmed cell death (PCD) is involved in the degradation of secretory cavity cells in the fruit, and that the spatio-temporal location of calcium is closely related to nuclear DNA degrada...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Bai M,Liang M,Huai B,Gao H,Tong P,Shen R,He H,Wu H

    更新日期:2020-08-06 00:00:00

  • Ionic currents and ion fluxes in Neurospora crassa hyphae.

    abstract::Voltage dependence of ionic currents and ion fluxes in a walled, turgor-regulating cell were measured in Neurospora crassa. The hyphal morphology of the model organism Neurospora simplifies cable analysis of ionic currents to determine current density for quantitative comparisons with ion fluxes. The ion fluxes were m...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Lew RR

    更新日期:2007-01-01 00:00:00

  • Tissue-specific laser microdissection of the Brassica napus funiculus improves gene discovery and spatial identification of biological processes.

    abstract::The three primary tissue systems of the funiculus each undergo unique developmental programs to support the growth and development of the filial seed. To understand the underlying transcriptional mechanisms that orchestrate development of the funiculus at the globular embryonic stage of seed development, we used laser...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Chan AC,Khan D,Girard IJ,Becker MG,Millar JL,Sytnik D,Belmonte MF

    更新日期:2016-05-01 00:00:00

  • Evidence for maternal control of seed size in maize from phenotypic and transcriptional analysis.

    abstract::Seed size is an important component of grain yield and a key determinant trait for crop domestication. The Krug Yellow Dent long-term selection experiment for large and small seed provides a valuable resource to dissect genetic and phenotypic changes affecting seed size within a common genetic background. In this stud...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Zhang X,Hirsch CN,Sekhon RS,de Leon N,Kaeppler SM

    更新日期:2016-03-01 00:00:00

  • Long-distance signalling from roots to shoots assessed: the flooding story.

    abstract::Several kinds of signal may be generated when roots are exposed to an environmental stress. Some, but not all, are conveyed to shoots in the transpiration stream. Principles are summarized that may help establish experimentally the presence or intensity of root signals transported by transpiration. In many dryland spe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Jackson MB

    更新日期:2002-02-01 00:00:00

  • Modulation of plant TPC channels by polyunsaturated fatty acids.

    abstract::Polyunsaturated fatty acids (PUFAs) are powerful modulators of several animal ion channels. It is shown here that PUFAs strongly affect the activity of the Slow Vacuolar (SV) channel encoded by the plant TPC1 gene. The patch-clamp technique was applied to isolated vacuoles from carrot taproots and Arabidopsis thaliana...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Gutla PV,Boccaccio A,De Angeli A,Gambale F,Carpaneto A

    更新日期:2012-10-01 00:00:00

  • Cotton metallothionein GhMT3a, a reactive oxygen species scavenger, increased tolerance against abiotic stress in transgenic tobacco and yeast.

    abstract::A cDNA clone encoding a 64-amino acid type 3 metallothionein protein, designated GhMT3a, was isolated from cotton (Gossypium hirsutum) by cDNA library screening. Northern blot analysis indicated that mRNA accumulation of GhMT3a was up-regulated not only by high salinity, drought, and low temperature stresses, but also...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Xue T,Li X,Zhu W,Wu C,Yang G,Zheng C

    更新日期:2009-01-01 00:00:00

  • Effects of short episodes of heat stress on flower production and fruit-set of groundnut (Arachis hypogaea L.).

    abstract::Groundnuts (Arachis hypogaea L.) are an important crop of the semi-arid tropics where they are often exposed to maximum temperatures of > 40 degrees C for short periods during the growing season. The objectives of this study were to determine: (i) the effects of short periods of exposure to high temperature on flower ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Prasad PV,Craufurd PQ,Summerfield RJ,Wheeler TR

    更新日期:2000-04-01 00:00:00

  • A perspective on crassulacean acid metabolism photosynthesis evolution of orchids on different continents: Dendrobium as a case study.

    abstract::Members of the Orchidaceae, one of the largest families of flowering plants, evolved the crassulacean acid metabolism (CAM) photosynthesis strategy. It is thought that CAM triggers adaptive radiation into new niche spaces, yet very little is known about its origin and diversification on different continents. Here, we ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Li MH,Liu DK,Zhang GQ,Deng H,Tu XD,Wang Y,Lan SR,Liu ZJ

    更新日期:2019-11-29 00:00:00

  • Nitrate, NO and haemoglobin in plant adaptation to hypoxia: an alternative to classic fermentation pathways.

    abstract::The role of nitrate reduction to produce nitric oxide (NO) and its subsequent oxidation by oxyhaemoglobin as a mechanism to maintain plant cell energetics during hypoxia is examined. Nitrate reduction in hypoxic conditions can be considered as an alternative respiratory pathway, with nitrate as an intermediate electro...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Igamberdiev AU,Hill RD

    更新日期:2004-12-01 00:00:00

  • Meiotic cytology and chromosome behaviour in wild-type Arabidopsis thaliana.

    abstract::This article reviews the historical development of cytology and cytogenetics in Arabidopsis, and summarizes recent developments in molecular cytogenetics, with special emphasis on meiotic studies. Despite the small genome and small chromosomes of Arabidopsis, considerable progress has been made in developing appropria...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Armstrong SJ,Jones GH

    更新日期:2003-01-01 00:00:00

  • Elucidation of enzymes involved in the biosynthetic pathway of bioactive polyacetylenes in Bidens pilosa using integrated omics approaches.

    abstract::Polyacetylene (PA) compounds from Bidens pilosa are known to have several pharmacological activities. In this study, we aimed to elucidate the major genes encoding enzymes involved in the biosynthesis of PA in B. pilosa. Seven PA metabolites present in B. pilosa leaves were found to be induced by methyl jasmonate (MeJ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Chung HH,Ting HM,Wang WH,Chao YT,Hsieh CH,Apaya MK,Sung YC,Lin SS,Hwu FY,Shyur LF

    更新日期:2020-10-16 00:00:00

  • Multispectral fluorescence and reflectance imaging at the leaf level and its possible applications.

    abstract::Images taken at different spectral bands are increasingly used for characterizing plants and their health status. In contrast to conventional point measurements, imaging detects the distribution and quantity of signals and thus improves the interpretation of fluorescence and reflectance signatures. In multispectral fl...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Lenk S,Chaerle L,Pfündel EE,Langsdorf G,Hagenbeek D,Lichtenthaler HK,Van Der Straeten D,Buschmann C

    更新日期:2007-01-01 00:00:00

  • Regulation of mitochondrial calcium in plants versus animals.

    abstract::Ca(2+) acts as an important cellular second messenger in eukaryotes. In both plants and animals, a wide variety of environmental and developmental stimuli trigger Ca(2+) transients of a specific signature that can modulate gene expression and metabolism. In animals, mitochondrial energy metabolism has long been consid...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Wagner S,De Bortoli S,Schwarzländer M,Szabò I

    更新日期:2016-06-01 00:00:00

  • The multi-protein family of Arabidopsis sulphotransferases and their relatives in other plant species.

    abstract::All members of the sulphotransferase (SOT, EC 2.8.2.-) protein family use 3'-phosphoadenosine 5'-phosphosulphate (PAPS) as the sulphuryl donor and transfer the sulphonate group to an appropriate hydroxyl group of several classes of substrates. These enzymes have highly conserved domains and can be found in eubacteria ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Klein M,Papenbrock J

    更新日期:2004-08-01 00:00:00

  • Capitalizing on deliberate, accidental, and GM-driven environmental change caused by crop modification.

    abstract::The transgenic traits associated with the majority of commercial genetically modified crops are focused on improving herbicide and insecticide management practices. The use of the transgenic technology in these crops and the associated chemistry has been the basis of studies that provide evidence for occasional improv...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Knox OG,Walker RL,Booth EJ,Hall C,Crossan AN,Gupta VV

    更新日期:2012-01-01 00:00:00

  • Sugar regulation of SUGAR TRANSPORTER PROTEIN 1 (STP1) expression in Arabidopsis thaliana.

    abstract::Sugars regulate the expression of many genes at the transcriptional level. In Arabidopsis thaliana, sugars induce or repress the expression of >1800 genes, including the STP1 (SUGAR TRANSPORTER PROTEIN 1) gene, which encodes an H(+)/monosaccharide cotransporter. STP1 transcript levels decrease more rapidly after the a...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Cordoba E,Aceves-Zamudio DL,Hernández-Bernal AF,Ramos-Vega M,León P

    更新日期:2015-01-01 00:00:00

  • The juxtamembrane and carboxy-terminal domains of Arabidopsis PRK2 are critical for ROP-induced growth in pollen tubes.

    abstract::Polarized growth of pollen tubes is a critical step for successful reproduction in angiosperms and is controlled by ROP GTPases. Spatiotemporal activation of ROP (Rho GTPases of plants) necessitates a complex and sophisticated regulatory system, in which guanine nucleotide exchange factors (RopGEFs) are key components...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Zhao XY,Wang Q,Li S,Ge FR,Zhou LZ,McCormick S,Zhang Y

    更新日期:2013-12-01 00:00:00

  • Involvement of Arabidopsis phospholipase D δ in regulation of ROS-mediated microtubule organization and stomatal movement upon heat shock.

    abstract::Reactive oxygen species (ROS) are plant metabolic and signaling molecules involved in responses to various external stresses, but the existence of ROS receptors and how plants respond to ROS remain largely unknown. Here we report that the plasma membrane-localized phospholipase D δ (PLDδ) protein is crucial for sensin...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Song P,Jia Q,Chen L,Jin X,Xiao X,Li L,Chen H,Qu Y,Su Y,Zhang W,Zhang Q

    更新日期:2020-10-22 00:00:00

  • Activity and protein level of AO isoforms in pea plants (Pisum sativum L.) during vegetative development and in response to stress conditions.

    abstract::Among three AO isoforms detected in pea plants, the activity of PAO-1 was dominant in leaves of seedlings and young leaves of mature plants, while PAO-3 revealed the highest band intensity in old leaves and roots. PAO-1 and PAO-3 are homodimers consisting of 145 kDa and 140 kDa subunits, respectively, while PAO-2 is a...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Zdunek-Zastocka E,Omarov RT,Koshiba T,Lips HS

    更新日期:2004-06-01 00:00:00