Overexpression of hyaluronan synthase-2 reduces the tumorigenic potential of glioma cells lacking hyaluronidase activity.


OBJECTIVE:The interactions of CD44 with hyaluronan are thought to be crucial for tumor cell attachment to the extracellular matrix, migration, and invasion. For migration to occur, however, the interactions between hyaluronan and cell surface receptors need to be transient. Hyaluronidases may facilitate the degradation of hyaluronan bound to the cell surface and thus reduce the interactions of the cells with the matrix, whereas the overproduction of hyaluronan in the absence of hyaluronidase activity may prevent cells from proliferating or invading normal surrounding tissue. METHODS:We analyzed the effects in vitro and in vivo of hyaluronan synthase-2 (HAS2) overexpression on a murine glioma cell line that is deficient in hyaluronidase activity. In addition, we evaluated the expression levels of HAS and hyaluronidase genes in human glioma cell lines and in glioma specimens. RESULTS:Increased hyaluronan synthesis had no effect on the in vitro proliferation of the cells but diminished their in vivo growth rate. Several human glioma cell lines were found to overexpress hyaluronan synthases, but they did so in conjunction with hyaluronidase Hyal2 and MGEA5 expression. Similarly, all glioblastomas multiforme expressed hyaluronidases MGEA5 and Hyal2. CONCLUSION:The data suggest that an increased synthesis of hyaluronan by astrocytoma cells is only promoting tumor cell growth in vivo if the cells express hyaluronidases as well.






Enegd B,King JA,Stylli S,Paradiso L,Kaye AH,Novak U





Has Abstract


2002-06-01 00:00:00












  • Development of a unique phantom to assess the geometric accuracy of magnetic resonance imaging for stereotactic localization.

    abstract:OBJECTIVE:To test the spatial accuracy of coordinates generated from magnetic resonance imaging (MRI) scans, using the Brown-Roberts-Wells head frame and localizer system (Radionics, Inc., Burlington, MA). METHODS:An anthropomorphic head phantom, consisting of a two-dimensional lattice of acrylic spheres (4-mm diamete...


    pub_type: 杂志文章


    authors: Orth RC,Sinha P,Madsen EL,Frank G,Korosec FR,Mackie TR,Mehta MP

    更新日期:1999-12-01 00:00:00

  • Pipeline embolization device for the treatment of intra- and extracranial fusiform and dissecting aneurysms: initial experience and long-term follow-up.

    abstract:BACKGROUND:Flow-diverting stents offer a promising treatment option for complex aneurysms. OBJECTIVE:To evaluate the safety and efficacy of the Pipeline embolization device (PED) in the treatment of fusiform and dissecting aneurysms. METHODS:Sixty-five consecutive patients with 69 fusiform and dissecting aneurysms un...


    pub_type: 杂志文章


    authors: Fischer S,Perez MA,Kurre W,Albes G,Bäzner H,Henkes H

    更新日期:2014-10-01 00:00:00

  • Surgical mortality at 30 days and complications leading to recraniotomy in 2630 consecutive craniotomies for intracranial tumors.

    abstract:BACKGROUND:In order to weigh the risks of surgery against the presumed advantages, it is important to have specific knowledge about complication rates. OBJECTIVE:To study the surgical mortality and rate of reoperations for hematomas and infections after intracranial surgery for brain tumors in a large, contemporary, s...


    pub_type: 杂志文章


    authors: Lassen B,Helseth E,Rønning P,Scheie D,Johannesen TB,Mæhlen J,Langmoen IA,Meling TR

    更新日期:2011-05-01 00:00:00

  • Cerebral arterial revascularization: the value of repeated angiography in selection of patients for operation.

    abstract::Three patients with occlusive cerebrovascular disease were evaluated for possible extracranial-intracranial arterial anastomosis. Cerebral angiography repeated at a later date revealed disappearance of the initial vascular stenosis or occlusion and development of extensive collateral circulation. It was concluded in t...


    pub_type: 杂志文章


    authors: Fox JL

    更新日期:1978-05-01 00:00:00

  • The role of neuroendovascular therapy for the treatment of brain arteriovenous malformations.

    abstract::Neuroendovascular embolization represents a critical component of the multidisciplinary management of cerebral arteriovenous malformations. Safe and effective embolization may be performed only in the context of a well-designed, rational treatment plan that is fundamentally based on a clear understanding of the natura...


    pub_type: 杂志文章,评审


    authors: Fiorella D,Albuquerque FC,Woo HH,McDougall CG,Rasmussen PA

    更新日期:2006-11-01 00:00:00

  • Repeated endovascular coil occlusion in 350 of 2759 intracranial aneurysms: safety and effectiveness aspects.

    abstract:OBJECTIVE:Evaluation of the safety and effectiveness of endovascular retreatment of intracranial aneurysms with detachable coils. METHODS:Retrospective analysis of clinical outcome as well as angiographic studies both before and after coil retreatment. The indication for retreatment was based on either inadequate occl...


    pub_type: 杂志文章


    authors: Henkes H,Fischer S,Liebig T,Weber W,Reinartz J,Miloslavski E,Kühne D

    更新日期:2008-06-01 00:00:00

  • New endoscope shaft for endoscopic transsphenoidal pituitary surgery.

    abstract:OBJECTIVE:To describe a new endoscope shaft developed for suction-aspiration during endoscopic transsphenoidal pituitary surgery. METHODS:A custom-made shaft for a Wolf endoscope (Richard Wolf GmbH, Knittlingen, Germany) was developed with a height of 10 mm and a width of 5 mm, allowing an additional working channel f...


    pub_type: 杂志文章


    authors: van Lindert EJ,Grotenhuis JA

    更新日期:2005-07-01 00:00:00

  • Fiducial registration with spoiled gradient-echo magnetic resonance imaging enhances the accuracy of subthalamic nucleus targeting.

    abstract:BACKGROUND:A variety of imaging strategies may be used to derive reliable stereotactic coordinates when performing deep brain stimulation lead implants. No single technique has yet proved optimal. OBJECTIVE:To compare the relative accuracy of stereotactic coordinates for the subthalamic nucleus (STN) derived either fr...


    pub_type: 杂志文章


    authors: Ben-Haim S,Gologorsky Y,Monahan A,Weisz D,Alterman RL

    更新日期:2011-10-01 00:00:00

  • Intended Near-Total Removal of Koos Grade IV Vestibular Schwannomas: Reconsidering the Treatment Paradigm.

    abstract:BACKGROUND:The goals of treating Koos grade IV vestibular schwannomas are to relieve brainstem compression, preserve or restore neurological function, and achieve long-term tumor control while minimizing tumor- and treatment-related morbidity. OBJECTIVE:To propose a treatment paradigm involving the intentional near-to...


    pub_type: 杂志文章


    authors: Zumofen DW,Guffi T,Epple C,Westermann B,Krähenbühl AK,Zabka S,Taub E,Bodmer D,Mariani L

    更新日期:2018-02-01 00:00:00

  • Dorsal brain stem lipomas: case report.

    abstract::Intracranial lipomas are uncommon and rarely symptomatic lesions accounting for 0.06 to 0.46% of intracranial lesions. The management of symptomatic dorsal brain stem lipomas was once limited to cerebrospinal fluid diversion, but with recent advances in microsurgery, they now may be directly treated. We report three p...


    pub_type: 杂志文章


    authors: Baeesa SS,Higgins MJ,Ventureyra EC

    更新日期:1996-05-01 00:00:00

  • Surgical Performance Determines Functional Outcome Benefit in the Minimally Invasive Surgery Plus Recombinant Tissue Plasminogen Activator for Intracerebral Hemorrhage Evacuation (MISTIE) Procedure.

    abstract:BACKGROUND:Minimally invasive surgery procedures, including stereotactic catheter aspiration and clearance of intracerebral hemorrhage (ICH) with recombinant tissue plasminogen activator hold a promise to improve outcome of supratentorial brain hemorrhage, a morbid and disabling type of stroke. A recently completed Pha...


    pub_type: 杂志文章,随机对照试验


    authors: Awad IA,Polster SP,Carrión-Penagos J,Thompson RE,Cao Y,Stadnik A,Money PL,Fam MD,Koskimäki J,Girard R,Lane K,McBee N,Ziai W,Hao Y,Dodd R,Carlson AP,Camarata PJ,Caron JL,Harrigan MR,Gregson BA,Mendelow AD,Zuccare

    更新日期:2019-06-01 00:00:00

  • Long-term survival in patients with malignant astrocytoma.

    abstract::From 1978 to 1988, 314 patients with malignant astrocytoma were treated by our neuro-oncology team. Twenty-five patients were excluded from further analysis because of a lack of adequate follow-up, the brain-stem location of the tumor, or an age of less than 18 years. Of the 289 remaining patients in the valid study g...


    pub_type: 杂志文章


    authors: Salcman M,Scholtz H,Kaplan RS,Kulik S

    更新日期:1994-02-01 00:00:00

  • Treatment of rhinocerebral mucormycosis with intravenous interstitial, and cerebrospinal fluid administration of amphotericin B: case report.

    abstract:IMPORTANCE:Rhinocerebral mucormycosis is extremely difficult to treat. Approximately 70% of patients are poorly controlled diabetics, and many of the remainder are immunocompromised as a consequence of cytotoxic drugs, burn injuries, or end-stage renal disease. Despite standard treatment consisting of surgical debridem...


    pub_type: 杂志文章


    authors: Adler DE,Milhorat TH,Miller JI

    更新日期:1998-03-01 00:00:00

  • The association between weather and spontaneous subarachnoid hemorrhage: an analysis of 155 US hospitals.

    abstract:BACKGROUND:A seasonal and meteorological influence on the incidence of spontaneous subarachnoid hemorrhage (SAH) has been suggested, but a consensus in the literature has yet to emerge. OBJECTIVE:This study examines the impact of weather patterns on the incidence of SAH using a geographically broad analysis of hospita...


    pub_type: 杂志文章


    authors: Cowperthwaite MC,Burnett MG

    更新日期:2011-01-01 00:00:00

  • Suspension laminotomy: a new surgical technique for compression myelopathy.

    abstract::In an attempt to eliminate the many negative aspects of conventional extensive laminectomy, we originated in 1981 and have developed a new surgical technique called "suspension laminotomy." A basic principle of this operation is preservation and repair of the soft and bony tissue of the spine with osseous enlargement ...


    pub_type: 杂志文章


    authors: Ohmori K,Ishida Y,Suzuki K

    更新日期:1987-12-01 00:00:00

  • Gamma knife radiosurgery for cerebellopontine angle meningiomas: a multicenter study.

    abstract:BACKGROUND:Resection of cerebellopontine angle (CPA) meningiomas may result in significant neurological morbidity. Radiosurgery offers a minimally invasive alternative to surgery. OBJECTIVE:To evaluate, in a multicenter cohort study, the outcomes of patients harboring CPA meningiomas who underwent Gamma Knife radiosur...


    pub_type: 杂志文章,多中心研究


    authors: Ding D,Starke RM,Kano H,Nakaji P,Barnett GH,Mathieu D,Chiang V,Omay SB,Hess J,McBride HL,Honea N,Lee JY,Rahmathulla G,Evanoff WA,Alonso-Basanta M,Lunsford LD,Sheehan JP

    更新日期:2014-10-01 00:00:00

  • Natural history of brainstem cavernous malformations.

    abstract:OBJECTIVE:To review the natural history and determine the rates of intra- and extralesional hemorrhaging of brainstem cavernous malformations (cavernomas) monitored by one neuro-ophthalmology service. METHODS:A record review of all patients with brainstem cavernomas who were evaluated by a neuroophthalmology service b...


    pub_type: 杂志文章


    authors: Kupersmith MJ,Kalish H,Epstein F,Yu G,Berenstein A,Woo H,Jafar J,Mandel G,De Lara F

    更新日期:2001-01-01 00:00:00

  • Embolization of arteriovenous malformations with Onyx: clinicopathological experience in 23 patients.

    abstract:OBJECTIVE:To report our experience in treatment of arteriovenous malformations (AVMs) using a new liquid embolic agent, Onyx (Micro Therapeutics, Inc., Irvine, CA). METHODS:Between January 1998 and May 1999, 23 patients (8 men and 15 women) were treated. The patients' average age was 40 years, with seizure being the m...


    pub_type: 杂志文章


    authors: Jahan R,Murayama Y,Gobin YP,Duckwiler GR,Vinters HV,Viñuela F

    更新日期:2001-05-01 00:00:00

  • Predictors of Poor Quality of Life 1 Year After Subarachnoid Hemorrhage.

    abstract:BACKGROUND:Risk factors for poor quality of life (QOL) after subarachnoid hemorrhage (SAH) remain poorly described. OBJECTIVE:To identify the frequency and predictors of poor QOL 1 year after SAH. METHODS:We studied 1-year QOL in a prospectively collected cohort of 1181 consecutively admitted SAH survivors between Ju...


    pub_type: 杂志文章


    authors: Taufique Z,May T,Meyers E,Falo C,Mayer SA,Agarwal S,Park S,Connolly ES,Claassen J,Schmidt JM

    更新日期:2016-02-01 00:00:00

  • Current status of carotid endarterectomy for atheromatous disease.

    abstract::The authors discuss the indications for both elective and emergency carotid endarterectomy. Reports on the surgical treatment of asymptomatic bruit and contralateral carotid stenosis are reviewed. The results of endarterectomy for symptomatic carotid disease, including transient ischemic attacks, acute neurological de...


    pub_type: 杂志文章,评审


    authors: Loftus CM,Quest DO

    更新日期:1983-12-01 00:00:00

  • High-resolution magnetic resonance imaging is a noninvasive method of observing injury and recovery in the peripheral nervous system.

    abstract:OBJECTIVE:Noninvasive observation of degenerating and regenerating peripheral nerves could improve the diagnosis and treatment of nerve injuries. We constructed a novel phased-array radiofrequency coil to permit magnetic resonance imaging (MRI) observation of the sciatic nerve and its target muscles in rats after injur...


    pub_type: 杂志文章


    authors: Aagaard BD,Lazar DA,Lankerovich L,Andrus K,Hayes CE,Maravilla K,Kliot M

    更新日期:2003-07-01 00:00:00

  • Trigeminal Nerve Compression Without Trigeminal Neuralgia: Intraoperative vs Imaging Evidence.

    abstract:BACKGROUND:While high-resolution imaging is increasingly used in guiding decisions about surgical interventions for the treatment of trigeminal neuralgia, direct assessment of the extent of vascular contact of the trigeminal nerve is still considered the gold standard for the determination of whether nerve decompressio...


    pub_type: 杂志文章


    authors: Jani RH,Hughes MA,Gold MS,Branstetter BF,Ligus ZE,Sekula RF Jr

    更新日期:2019-01-01 00:00:00

  • Novel device and technique for minimally invasive intracerebral hematoma evacuation in the same setting of a ruptured intracranial aneurysm: combined treatment in the neurointerventional angiography suite.

    abstract:BACKGROUND:The presence of intracerebral hematoma from aneurysm rupture is an indication for craniotomy for clot evacuation and aneurysm clipping. Some centers have begun securing aneurysms with coil embolization followed by clot evacuation in the operating room. This approach requires transporting a patient from the a...


    pub_type: 杂志文章


    authors: Turner RD,Vargas J,Turk AS,Chaudry MI,Spiotta AM

    更新日期:2015-03-01 00:00:00

  • Interstitial irradiation of brain tumors: a review.

    abstract::As an adjuvant to surgery, radiation therapy has consistently proven to be the most successful form of treatment for primary and secondary malignant brain tumors and possibly for inoperable benign tumors. Because the risk of radiation necrosis of normal brain limits the amount of radiation that can be given by externa...


    pub_type: 杂志文章,评审


    authors: Bernstein M,Gutin PH

    更新日期:1981-12-01 00:00:00

  • Endoscope-assisted supraorbital craniotomy for lesions of the interpeduncular fossa.

    abstract:OBJECTIVE:The supraorbital approach is well accepted for lesions in the anterior fossa, the sellar region, and the anterior circle of Willis. However, the usefulness of this approach has not yet been elucidated for lesions in the interpeduncular fossa. The technique of an endoscope-assisted, ipsi- and contralateral sup...


    pub_type: 杂志文章


    authors: Menovsky T,Grotenhuis JA,de Vries J,Bartels RH

    更新日期:1999-01-01 00:00:00

  • A new type of prehensile suction device.

    abstract::The authors describe a new suction device with prehensile properties that has proved itself particularly useful in the removal of certain types of tumor, especially extramedullary. ...


    pub_type: 杂志文章


    authors: Cantore G,Ciappetta P

    更新日期:1987-10-01 00:00:00

  • Neuronavigational endoscopic endonasal sellar and parasellar surgery using a 2-mm-diameter lens rigid-rod endoscope: a cadaver study.

    abstract:OBJECTIVE:Most of the endoscopes used for endonasal transsphenoidal surgery use 4-mm diameter lenses. The applicability of a newly developed neuroendoscope with a lens diameter of only 2 mm was tested in endonasal transsphenoidal pituitary surgery. METHODS:The newly developed rigid-rod neuroendoscope with a 2-mm lens ...


    pub_type: 杂志文章


    authors: Di Rocco F,Oi S,Samii A,Paternó V,Feigl GC,Lüdemann W,Samii M

    更新日期:2007-04-01 00:00:00

  • Craniocerebral injuries from dog bite in an infant.

    abstract::Animal bite is a common cause of craniofacial injuries in children. Whereas scalp lacerations from animal bites are often extensive and severe, only four instances of compound depressed skull fractures from animal bites have been reported. A case of a dog bite to the head of an infant is presented to point out the pot...


    pub_type: 杂志文章


    authors: Wilberger JE Jr,Pang D

    更新日期:1981-10-01 00:00:00

  • Inhibitory effects of sodium butyrate on proliferation and invasiveness of human glioma cells.

    abstract:OBJECTIVE:Sodium butyrate (SB), a differentiation-inducing agent, has been demonstrated to inhibit cellular proliferation in a number of human cell lines. Its precise mechanisms remain to be clarified, however. We investigated molecular mechanisms of SB-induced growth suppression as well as the effects of SB on the inv...


    pub_type: 杂志文章


    authors: Ito N,Sawa H,Nagane M,Noguchi A,Hara M,Saito I

    更新日期:2001-08-01 00:00:00

  • Metastasis of a rectal carcinoid to the posterior fossa.

    abstract::We report the metastasis of a rectal carcinoid to the dura mater of the posterior fossa. The clinical presentation was unusual because of a 10-year latent period between resection of the primary rectal tumor and symptoms and signs of the posterior fossa metastasis; the radiological findings were atypical of a metastas...


    pub_type: 杂志文章


    authors: Bouldin TW,Killebrew K,Boone SC,Gay RM

    更新日期:1979-10-01 00:00:00