Multiple rearrangements of mitochondrial DNA in unfertilized human oocytes.


OBJECTIVE:To determine the rearrangement of mitochondrial DNA (mtDNA) in unfertilized human oocytes and compromised embryos to evaluate the fertilization capacity of oocytes. DESIGN:Prospective laboratory research. SETTING:IVF laboratory in a university hospital. PATIENT(S):One hundred twenty-four unfertilized oocytes, 98 arrested embryos, and 45 tripronucleate (3PN) embryos from 65 female patients undergoing in vitro fertilization (IVF). INTERVENTION(S):Unfertilized oocytes and poor quality embryos were collected 48 hours after IVF. MAIN OUTCOME MEASURE(S):Comparison of the frequency of mtDNA deletions and fertilization rates of oocytes. RESULT(S):Multiple deletions of mtDNA were found in unfertilized oocytes and arrested embryos obtained from IVF patients. A 4977-bp deletion was the most frequent deletion in human oocytes and embryos. About 66.1% of the unfertilized oocytes, 34.8% of the arrested or fragmented embryos, and 21.1% of the 3PN embryos harbored the 4977-bp deletion of mtDNA. There was a significant increase in the proportion of deleted mtDNA in unfertilized oocytes. CONCLUSION(S):Accumulation of mtDNA deletions may contribute to mitochondrial dysfunction and impaired ATP production. We conclude that the accumulation of rearranged mtDNA may interfere with fertilization of human oocytes and further embryonic development.


Fertil Steril


Fertility and sterility


Hsieh RH,Tsai NM,Au HK,Chang SJ,Wei YH,Tzeng CR





Has Abstract


2002-05-01 00:00:00














  • Pregnancy and birth rates after oocyte donation.

    abstract:OBJECTIVE:To determine accumulated conception and live birth rates in ovum donation. DESIGN:Retrospective study from a computer database. Pregnancies with one gestational sac observed by ultrasound have been included as conceptional cycles and pregnancies that resulted in one live child were recorded for the analysis ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Remohí J,Gartner B,Gallardo E,Yalil S,Simón C,Pellicer A

    更新日期:1997-04-01 00:00:00

  • Normal pregnancies resulting from testicular sperm extraction and intracytoplasmic sperm injection for azoospermia due to maturation arrest.

    abstract:OBJECTIVE:To see whether testicular sperm extraction could be used to perform intracytoplasmic sperm injection (ICSI) for men with nonobstructive azoospermia caused by maturation arrest. DESIGN:Uncontrolled prospective trial of an attempt to find occasional elongated spermatids or spermatozoa in testes of azoospermic ...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章


    authors: Silber SJ,van Steirteghem A,Nagy Z,Liu J,Tournaye H,Devroey P

    更新日期:1996-07-01 00:00:00

  • Two successful spontaneous pregnancies in a patient with a primary primitive neuroectodermal tumor of the ovary.

    abstract:OBJECTIVE:To describe a patient with primary primitive neuroectodermal tumor of the ovary with two successful spontaneous pregnancies. DESIGN:Case report. SETTING:Tertiary center for gynecologic oncology. PATIENT(S):A 25-year-old woman with two spontaneous pregnancies 5 months after and 2 years after conservative tr...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Demirtas E,Guven S,Guven ES,Baykal C,Ayhan A

    更新日期:2004-03-01 00:00:00

  • Vitrification is a highly efficient method to cryopreserve human embryos in in vitro fertilization patients at high risk of developing ovarian hyperstimulation syndrome.

    abstract::This study aimed to evaluate the efficacy of embryo vitrification as an emergency procedure for patients at high risk of developing ovarian hyperstimulation syndrome (OHSS). A total of 69 embryos, derived from 24 patients for whom embryo transfer could not be performed because of the risk of developing OHSS, were vitr...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Selman H,Brusco GF,Fiorini F,Barnocchi N,Mariani M,El-Danasouri I

    更新日期:2009-04-01 00:00:00

  • Pregnancy after an unsuccessful oocyte donation cycle.

    abstract::Clearly, to provide adequate counseling to women with POF, information is critically needed concerning the background incidence of spontaneous improvement in ovarian function as well as carefully designed, controlled studies to evaluate the efficacy of presumed therapeutic intervention. The case presented herein under...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Santoro N,Schmidt CL

    更新日期:1990-01-01 00:00:00

  • Donation of fertilized uterine ova to infertile women.

    abstract::Nonsurgical recovery of a preimplantation conceptus from the uterus of a fertile donor woman with transfer to an infertile recipient woman and subsequent pregnancy has been described previously. In this report, we describe the performance of 56 nonsurgical uterine lavages, recovery of 23 ova, and transfer of 17 ova wi...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Formigli L,Formigli G,Roccio C

    更新日期:1987-01-01 00:00:00

  • Bacterial colonization with balloon uterine stent placement in the uterus for 30 days: a randomized controlled clinical trial.

    abstract:OBJECTIVE:To assess bacterial colonization following balloon uterine stent placement in the uterus for 30 days. DESIGN:Prospective randomized controlled study. SETTING:Tertiary medical center. PATIENT(S):Sixty-eight women scheduled for hysteroscopy. INTERVENTION(S):Women who were undergoing hysteroscopic surgery we...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,随机对照试验


    authors: Lin YH,Jang TN,Hwang JL,Huang LW,Seow KM,Hsieh BC,Huang CH

    更新日期:2015-02-01 00:00:00

  • Midfollicular anticardiolipin and antiphosphatidylserine antibody titers do not correlate with in vitro fertilization outcome.

    abstract:OBJECTIVE:To determine the prevalence of anticardiolipin and antiphophatidylserine antibodies in an IVF population and to correlate their presence and specific isotype with IVF cycle outcome. DESIGN:Retrospective clinical study using stored midfollicular sera for determination of antibody status. SETTING:University h...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Kowalik A,Vichnin M,Liu HC,Branch W,Berkeley AS

    更新日期:1997-08-01 00:00:00

  • Clinical application of comprehensive chromosomal screening at the blastocyst stage.

    abstract:OBJECTIVE:To evaluate a new strategy for comprehensive chromosome screening at the blastocyst stage. DESIGN:Clinical research study. SETTING:An IVF clinic and a specialist preimplantation genetic diagnosis laboratory. PATIENT(S):Forty-five infertile couples participated in the study. The mean maternal age was 37.7 y...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Schoolcraft WB,Fragouli E,Stevens J,Munne S,Katz-Jaffe MG,Wells D

    更新日期:2010-10-01 00:00:00

  • Successful pregnancy and delivery after transfer of a single blastocyst derived from a vitrified mature human oocyte.

    abstract:OBJECTIVE:To describe the first case of delivery after the transfer of a single blastocyst derived from a vitrified mature human oocyte. DESIGN:Case report. SETTING:Private assisted reproduction clinic. PATIENT(S):A normal 31-year-old woman. INTERVENTION(S):An unsuccessful attempt was made to extract sperm from the...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Kyono K,Fuchinoue K,Yagi A,Nakajo Y,Yamashita A,Kumagai S

    更新日期:2005-10-01 00:00:00

  • Polyglycolic acid/chitosan glue and apoptosis of endometriotic cells.

    abstract:OBJECTIVE:To induce apoptosis of endometriotic cells of patients with endometriosis. DESIGN:To demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis. SETTING:University hospital infertility center. PATIENT(S):Twelve women who visited the center for infertility th...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Wang YC,Fu RH,Hsieh HJ,Chao HT,Kao SH

    更新日期:2005-07-01 00:00:00

  • Impact of elagolix treatment on fatigue experienced by women with moderate to severe pain associated with endometriosis.

    abstract:OBJECTIVE:To evaluate the efficacy of elagolix, an oral GnRH antagonist, for the reduction of fatigue in women with moderate or severe endometriosis-associated pain. DESIGN:Randomized, double-blind, multicenter, placebo-controlled phase III trial. SETTING:Clinics. PATIENT(S):A total of 860 women treated with elagoli...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Surrey ES,Soliman AM,Agarwal SK,Snabes MC,Diamond MP

    更新日期:2019-08-01 00:00:00

  • Tubal pregnancy in a unicornuate uterus with rudimentary horn: a case report.

    abstract:OBJECTIVE:To report a case of tubal pregnancy in a unicornuate uterus with rudimentary horn on the side of the rudiment and its pathology. DESIGN:Case report. SETTING:University hospital. PATIENT:An 18-year-old woman, primigravida, with tubal pregnancy. INTERVENTION(S):Systemic administration of methotrexate, salpi...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Handa Y,Hoshi N,Yamada H,Wada S,Kudo M,Tsuda K,Sagawa T,Fujimoto S

    更新日期:1999-08-01 00:00:00

  • Effect of halving the daily dose of triptorelin at the start of ovarian stimulation on hormone serum levels and the outcome of in vitro fertilization.

    abstract::Halving the standard daily dose of triptorelin at the start of ovarian stimulation in down-regulated women stimulated with recombinant FSH is enough for pituitary suppression and was associated with higher LH serum concentrations in the follicular phase. However, this did not translate into higher serum concentrations...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章


    authors: Fábregues F,Peñarrubia J,Creus M,Casamitjana R,Vanrell JA,Balasch J

    更新日期:2005-03-01 00:00:00

  • In vitro effects of coital lubricants and synthetic and natural oils on sperm motility.

    abstract:OBJECTIVE:To evaluate the effects of coital lubricants and oils on sperm motility. DESIGN:Comparative prospective in vitro study. SETTING:University Andrology laboratory. PATIENT(S):Twenty-two normozoospermic donors. INTERVENTION(S):Semen samples were incubated in modified human tubal fluid (mHTF) control and in 10...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Sandhu RS,Wong TH,Kling CA,Chohan KR

    更新日期:2014-04-01 00:00:00

  • The uterus and fertility.

    abstract:OBJECTIVE:To review the current understanding of the role the uterus plays in embryo implantation and to outline congenital anomalies and acquired diseases that impact normal uterine function. DESIGN:The publications related to the embryo implantation, Mullerian anomalies, uterine polyps, uterine synechiae, and myomas...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Taylor E,Gomel V

    更新日期:2008-01-01 00:00:00

  • Novel and conventional embryo parameters as input data for artificial neural networks: an artificial intelligence model applied for prediction of the implantation potential.

    abstract:OBJECTIVE:To describe novel embryo features capable of predicting implantation potential as input data for an artificial neural network (ANN) model. DESIGN:Retrospective cohort study. SETTING:University-affiliated private IVF center. PATIENT(S):This study included 637 patients from the oocyte donation program who un...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Bori L,Paya E,Alegre L,Viloria TA,Remohi JA,Naranjo V,Meseguer M

    更新日期:2020-12-01 00:00:00

  • European view of subspecialty training on behalf of the European Society of Human Reproduction and Embryology (ESHRE).

    abstract::Specialist training in reproductive medicine within Europe continues to evolve. Recent revisions, updates, and initiatives have helped to refine the core educational needs for the specialist trainee. ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Calhaz-Jorge C,Feki A,Farquharson R

    更新日期:2015-07-01 00:00:00

  • Risk factors for extrauterine pregnancy in women using an intrauterine device.

    abstract:OBJECTIVE:To identify the risk factors for ectopic pregnancy (EP) in women using an intrauterine device (IUD). DESIGN:Case-control study. SETTING:Auvergne region (France). PATIENT(S):Women using an IUD and suffering EP (243 cases) or having an intrauterine pregnancy (140 controls). INTERVENTION(S):None. MAIN OUTCO...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Bouyer J,Rachou E,Germain E,Fernandez H,Coste J,Pouly JL,Job-Spira N

    更新日期:2000-11-01 00:00:00

  • Addition of eosin to the aniline blue assay to enhance detection of immature sperm histones.

    abstract:OBJECTIVES:To compare the aniline blue assay with and without eosin, and to correlate the results with pregnancy outcome after intracytoplasmic sperm injection (ICSI) procedure. DESIGN:A retrospective study. SETTING:University-based fertility center. PATIENT(S):One hundred thirty infertile patients. INTERVENTION(S)...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Wong A,Chuan SS,Patton WC,Jacobson JD,Corselli J,Chan PJ

    更新日期:2008-11-01 00:00:00

  • In vitro fertilization outcomes after intracytoplasmic sperm injection with fresh or frozen-thawed testicular spermatozoa.

    abstract:OBJECTIVE:To compare the outcomes of intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) with fresh and cryopreserved testicular spermatozoa in patients with obstructive and nonobstructive azoospermia. DESIGN:Retrospective analysis of consecutive ICSI cycles. SETTING:Large urban reproductive medi...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Habermann H,Seo R,Cieslak J,Niederberger C,Prins GS,Ross L

    更新日期:2000-05-01 00:00:00

  • Detection of endometriosis with the use of plasma protein profiling by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry.

    abstract::In this prospective case-control study, we determined the role of surface-enhanced laser desorption and ionization time-of-flight mass spectrometry in the detection of histologically proven endometriosis. In the plasma of the group with endometriosis, there were 20 different protein peaks, and the classifier showed a ...

    journal_title:Fertility and sterility

    pub_type: 信件


    authors: Liu H,Lang J,Zhou Q,Shan D,Li Q

    更新日期:2007-04-01 00:00:00

  • Pentoxifylline is not useful in enhancing sperm function in cases with previous in vitro fertilization failure.

    abstract:OBJECTIVE:To assess the efficiency of the addition of pentoxifylline to spermatozoa for in vitro fertilization (IVF) in cases with previous fertilization failure. DESIGN:After retrieval, oocytes were divided at random and inseminated with spermatozoa prepared with or without pentoxifylline. SETTING:In vitro fertiliza...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Tournaye H,Janssens R,Camus M,Staessen C,Devroey P,Van Steirteghem A

    更新日期:1993-01-01 00:00:00

  • Misconduct in third-party assisted reproduction: a committee opinion.

    abstract::Physicians who are told or discover information that would be material to another party's participation in an assisted reproductive technology (ART) arrangement (such as lawyer's, donor's, gestational carrier's, or intended parent's) should encourage disclosure to that party. In some instances, it is ethically and leg...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Ethics Committee of the American Society for Reproductive Medicine.

    更新日期:2014-01-01 00:00:00

  • Minimum standards for practices offering assisted reproductive technologies: a committee opinion.

    abstract::This document is designed to provide a framework for assisted reproductive technology (ART) programs that meet or exceed the requirements suggested by the Centers for Disease Control and Prevention (CDC) for certification of ART laboratories. This document replaces the document, "Revised Minimum Standards for Practices...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,实务指引


    authors: Practice Committee of the American Society for Reproductive Medicine.,Practice Committee of the Society for Assisted Reproductive Technology.,Practice Committee of the Society of Reproductive Biologists and Technologists. Electronic address: asrm@as

    更新日期:2020-03-01 00:00:00

  • Nonapoptotic effects of tumor necrosis factor-related apoptosis-inducing ligand on interleukin-6, leukemia inhibitory factor, interleukin-8, and monocyte chemoattractant protein 1 vary between undifferentiated and decidualized human endometrial stromal ce

    abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which is known to exert death-inducing as well as nonapoptotic functions, has been shown to be expressed by the early trophoblast. Here we report that TRAIL has no apoptotic effects on human endometrial stromal cells, but differentially regulates cytokin...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Fluhr H,Sauter G,Steinmüller F,Licht P,Zygmunt M

    更新日期:2009-10-01 00:00:00

  • Obstetric and perinatal outcomes of pregnancies conceived with embryos cultured in a time-lapse monitoring system.

    abstract:OBJECTIVE:To compare obstetric and perinatal outcomes of singleton pregnancies resulting from embryos incubated in a time-lapse system (TLS) with those of embryos grown in standard IVF incubators (SI). DESIGN:Retrospective description of a cohort of patients who conceived during a randomized, controlled trial. SETTIN...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,随机对照试验


    authors: Insua MF,Cobo AC,Larreategui Z,Ferrando M,Serra V,Meseguer M

    更新日期:2017-09-01 00:00:00

  • A microliter oxygen electrode system for sperm suspensions.

    abstract::A new polarographic oxygen electrode system is described which can accept sperm suspensions with volumes as low as 50 microliters. In this configuration, the electrode tip forms the base of the suspension reaction chamber, and there is no stirring. Consequently, structured fluids such as cervical mucus can be applied....

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Katz DF,Blake ET,Mills RN,Fatt I

    更新日期:1978-12-01 00:00:00

  • The role of tubal reconstructive surgery in the era of assisted reproductive technologies.

    abstract::Tubal reconstructive surgery has fewer indications in the era of assisted reproductive technologies than in the past, but is still appropriate and effective treatment for properly selected individuals. ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Practice Committee of American Society for Reproductive Medicine.

    更新日期:2008-11-01 00:00:00

  • Subcutaneous gonadotropin therapy in male patients with hypogonadotropic hypogonadism.

    abstract:OBJECTIVE:The response to subcutaneous (SC) gonadotropin replacement therapy, using human chorionic gonadotropin (hCG) and human menopausal gonadotropin (hMG) or hCG alone, was evaluated in male hypothalamic hypogonadism. DESIGN:Sixteen patients with hypothalamic hypogonadism were treated with gonadotropins for induct...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Saal W,Happ J,Cordes U,Baum RP,Schmidt M

    更新日期:1991-08-01 00:00:00