Abstract：
OBJECTIVE:To determine the rearrangement of mitochondrial DNA (mtDNA) in unfertilized human oocytes and compromised embryos to evaluate the fertilization capacity of oocytes. DESIGN:Prospective laboratory research. SETTING:IVF laboratory in a university hospital. PATIENT(S):One hundred twenty-four unfertilized oocytes, 98 arrested embryos, and 45 tripronucleate (3PN) embryos from 65 female patients undergoing in vitro fertilization (IVF). INTERVENTION(S):Unfertilized oocytes and poor quality embryos were collected 48 hours after IVF. MAIN OUTCOME MEASURE(S):Comparison of the frequency of mtDNA deletions and fertilization rates of oocytes. RESULT(S):Multiple deletions of mtDNA were found in unfertilized oocytes and arrested embryos obtained from IVF patients. A 4977-bp deletion was the most frequent deletion in human oocytes and embryos. About 66.1% of the unfertilized oocytes, 34.8% of the arrested or fragmented embryos, and 21.1% of the 3PN embryos harbored the 4977-bp deletion of mtDNA. There was a significant increase in the proportion of deleted mtDNA in unfertilized oocytes. CONCLUSION(S):Accumulation of mtDNA deletions may contribute to mitochondrial dysfunction and impaired ATP production. We conclude that the accumulation of rearranged mtDNA may interfere with fertilization of human oocytes and further embryonic development.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Hsieh RH,Tsai NM,Au HK,Chang SJ,Wei YH,Tzeng CRdoi
10.1016/s0015-0282(02)02994-1keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
1012-7issue
5eissn
0015-0282issn
1556-5653pii
S0015028202029941journal_volume
77pub_type
杂志文章abstract：OBJECTIVE:To report preferences of recipient couples for genetic testing of their oocyte donors. DESIGN:Observational report of results from a genetic testing-options form implemented as part of routine care. SETTING:Private practice. PATIENT(S):Recipients and oocyte donors. INTERVENTION(S):Recipient couples comple...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.10.069
更新日期：2008-12-01 00:00:00
abstract：OBJECTIVE:To present a rare case of endometrial stromal sarcoma arising in endometriosis of the rectovaginal septum. DESIGN:Case report. SETTING:Academic tertiary referral center for endometriosis treatment. PATIENT(S):A 50-year-old woman with a history of endometriosis presented with catamenial rectal pain and deep...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.09.053
更新日期：2004-04-01 00:00:00
abstract：OBJECTIVE:To correlate apoptosis of cultured human granulosa-lutein cells (GL cells) with the outcome of IVF (embryo fragmentation and pregnancy rate) and to study the effect of insulin and insulin-like growth factor I (IGF-I) on apoptosis. DESIGN:In vitro assays. SETTING:University laboratory and private IVF center....
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.08.014
更新日期：2006-02-01 00:00:00
abstract：OBJECTIVE:To determine if estrogen ameliorates hot flashes by raising the core body temperature sweating threshold, by reducing core body temperature fluctuations, and/or by reducing sympathetic activation (as measured by plasma 3-methoxy-4-hydroxyphenylglycol). DESIGN:Laboratory physiological study. SETTING:Universi...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(01)03009-6
更新日期：2002-03-01 00:00:00
abstract：OBJECTIVE:To evaluate whether older first-time mothers (≥37 years) have higher rates of postpartum depression compared with younger first-time mothers, controlling for mode of conception and known risk factors for postpartum depression. DESIGN:Prospective cohort study. SETTING:Assisted reproductive technology (ART) c...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究
doi：10.1016/j.fertnstert.2011.08.037
更新日期：2011-11-01 00:00:00
abstract：:The comprehensive "one-stop shop" ultrasound evaluation of an infertile woman, performed around cycle days 5 to 9, will reveal abundant information about the anatomy and morphology of the pelvic organs and thereby avoid costly radiation and iodinated contrast exposure. We propose a two-dimensional and three-dimensiona...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2016.03.026
更新日期：2016-06-01 00:00:00
abstract：:The immediate and long term fertility after bilateral ovarian wedge resection in 53 women with clomiphene citrate-resistant hyperandrogenic chronic anovulation is related to the histology of the ovarian wedges. Patients with polycystic ovaries and hyperplastic stromal abnormalities had most spontaneous conceptions and...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60845-2
更新日期：1989-08-01 00:00:00
abstract：OBJECTIVE:To evaluate the incidence of monozygotic twinning (MZT) in pregnancies conceived after blastocyst transfer compared to cleavage-stage transfer. DESIGN:Retrospective study. SETTING:University IVF program. PATIENT(S):All IVF patients with viable pregnancies conceived during a 4-year period. INTERVENTION(S):...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)04754-4
更新日期：2003-03-01 00:00:00
abstract：:This monograph, written by the pioneers of IVF and reproductive medicine, celebrates the history, achievements, and medical advancements made over the last 40 years in this rapidly growing field. ...
journal_title：Fertility and sterility
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.fertnstert.2018.06.005
更新日期：2018-07-15 00:00:00
abstract：:The aim of the present review is to summarize recent developments in the field of urologic robotic surgery. A nonsystematic literature review was performed to retrieve publications related to robotic surgery in urology and evidence-based critical analysis was conducted by focusing on the literature of the past 5 years...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2014.05.033
更新日期：2014-10-01 00:00:00
abstract：OBJECTIVE:To compare the levonorgestrel intrauterine system (LNG-IUS) with hysterectomy in patients with adenomyosis and to study the effects of both treatments on quality of life (QOL). DESIGN:Prospective randomized clinical trial. SETTING:Women's health teaching and research hospital. PATIENT(S):Eighty-six patient...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2010.10.009
更新日期：2011-02-01 00:00:00
abstract：:No nerve fibers are found in endometriomas or in the endometrioma-containing ovaries. These findings are consistent with our previous report that no correlation exists between the presence of nerve fibers and endometriosis. ...
journal_title：Fertility and sterility
pub_type： 信件
doi：10.1016/j.fertnstert.2003.11.049
更新日期：2004-07-01 00:00:00
abstract：:The tenet that a combination of human follicle-stimulating hormone (hFSH)/human menopausal gonadotropin (hMG) improves follicular recruitment was assessed by randomly treating ovulatory women either with hFSH/hMG on days 3 and 4 of the cycle followed by two ampules of hMG daily or with a constant daily dose of 2 ampul...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)60315-1
更新日期：1988-11-01 00:00:00
abstract：OBJECTIVE:To reduce estrogen exposure in women with endometrial cancer undergoing in vitro fertilization using an aromatase inhibitor. DESIGN:Prospective case series. SETTING:Academic center for reproductive medicine. PATIENT(S):Endometrial carcinoma patients presenting for fertility preservation or fresh embryo tra...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.12.068
更新日期：2007-09-01 00:00:00
abstract：:In a prospective randomized fashion, this study evaluated embryo development in vitro after defragmentation versus assisted hatching alone of low grade day 3 embryos. Although a sustained decrease in day 5 fragmentation was observed in the defragmented group versus the assisted hatching only group, no difference in co...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.03.014
更新日期：2010-11-01 00:00:00
abstract：OBJECTIVE:To study the association between low percentages of intact sperm acrosomes and fertilization failures in conventional IVF procedures. DESIGN:A retrospective study. SETTING:Clinical and academic research environment. PATIENT(S):Patients undergoing treatment of infertility. INTERVENTION(S):Sperm cells were ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00201-0
更新日期：1999-07-01 00:00:00
abstract：:For determination of how exposure of the monkey follicle to the preovulatory luteinizing hormone (LH)/follicle-stimulating hormone (FSH) surge alters its responsiveness to FSH in terms of estrogen and progesterone secretory ability, monkey thecal tissue and granulosa cells were harvested prior to and during the midcyc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48087-8
更新日期：1984-09-01 00:00:00
abstract：OBJECTIVE:To determine whether differences existed in mood and coping styles among fertile men, oligoasthenospermic men, or euspermic men whose wives were undergoing ovulation stimulation with clomiphene and IUI. DESIGN:A cross-sectional research design. SETTING:Hospital-based academic fertility clinic. PATIENT(S):3...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(00)01511-9
更新日期：2000-10-01 00:00:00
abstract：OBJECTIVE:To determine whether preoperative hysteroscopic examination increases the risk for peritoneal dissemination of endometrial cancer cells and the effect of hysteroscopy on disease prognosis. DESIGN:Meta-analysis. SETTING:Literature search conducted via domestic and international databases for studies on preop...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析
doi：10.1016/j.fertnstert.2011.07.1146
更新日期：2011-10-01 00:00:00
abstract：OBJECTIVE:To evaluate the association between FSH efficacy and FSHR alleles. DESIGN:Retrospective study. SETTING:University-based fertility unit and a private center for biomedical research. PATIENT(S):One hundred two women with ovarian function who were undergoing controlled ovarian stimulation (COS). Women were ca...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(03)00795-7
更新日期：2003-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the feasibility and the efficacy of in vitro maturation (IVM) when immature oocyte collection was performed in the early follicular, late follicular, or luteal phases in women with cancer who require urgent chemotherapy. DESIGN:Retrospective cohort study. SETTING:University teaching hospital. P...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2016.09.041
更新日期：2017-01-01 00:00:00
abstract：OBJECTIVE:To review osteoporosis, a disease characterized by low bone mass, microarchitectural deterioration of bone tissue leading to increased bone fragility and a consequent increase in fracture risk. The disorder has become a major health problem in the West, where increased life expectancy has placed new emphasis ...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：
更新日期：1994-12-01 00:00:00
abstract：OBJECTIVE:To investigate danazol's effect in vitro and in vivo on the ability of peripheral blood monocytes (PBM) from women with endometriosis to stimulate endometrial cell proliferation. DESIGN:Uterine endometrial cells from untreated or danazol-treated patients with endometriosis were cultured with or without autol...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：OBJECTIVE:To compare the modifications on basal and post-methionine homocysteine (Hcy) levels induced by transdermal vs. oral continuous combined hormone therapy (HT). DESIGN:Prospective randomized study. SETTING:Outpatient service at university hospital. PATIENT(S):Twenty-four healthy postmenopausal women. INTERVE...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2003.05.026
更新日期：2004-01-01 00:00:00
abstract：OBJECTIVE:To study the use of the aromatase inhibitor letrozole for treatment of ectopic pregnancy compared with methotrexate. DESIGN:Nonrandomized prospective cohort study. SETTING:University hospital. PATIENT(S):A series of 42 consecutive patients with undisturbed ectopic pregnancy. INTERVENTION(S):Counseling on ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.04.001
更新日期：2020-08-01 00:00:00
abstract：OBJECTIVE:To evaluate the degree of enrichment of spermatogonial stem cells (SSCs) from human testicular cell cultures by ITGA6+, HLA-/ITGA6+, GPR125+, and HLA-/GPR125+ magnetic-assisted cell sorting (MACS). DESIGN:Experimental basic science study. SETTING:Reproductive biology laboratory. PATIENT(S):Multiple samples...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.04.022
更新日期：2014-08-01 00:00:00
abstract：:A surrogate end point (e.g., a laboratory test or image) serves as a proxy for a clinical end point of importance (e.g., fracture, thrombosis, or death). Adoption and use of surrogate end points lacking validation, especially in cardiovascular medicine, have caused thousands of patients' deaths, a serious violation of...
journal_title：Fertility and sterility
pub_type： 社论,评审
doi：10.1016/j.fertnstert.2009.12.054
更新日期：2010-04-01 00:00:00
abstract：:Elevation of serum luteinizing hormone levels was observed in five normal male volunteers while they were taking Centchroman. This effect resembles that of clomiphene and suggests a new biologic potential of Centchroman. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)41785-1
更新日期：1976-04-01 00:00:00
abstract：OBJECTIVE:To investigate the relationship between economic activities, insurance mandates, and the use of in vitro fertilization (IVF) in the United States. DESIGN:We examined the correlation between the coincident index (a proxy for overall economic conditions) and IVF use at the national level from 2000 to 2011. We ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.10.042
更新日期：2015-02-01 00:00:00
abstract：:A double-blind randomized study was performed in two groups of eight normally cycling patients: group I received 10 mg/d of RU486 for 4 days from the date of ovulation and group II received a placebo. On day +5, cytosol and endometrial estrogen receptors (ERs), and progesterone receptors (PRs) were analyzed by radioli...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)54183-1
更新日期：1991-03-01 00:00:00
