Multi site polyadenylation and transcriptional response to stress of a vacuolar type H+-ATPase subunit A gene in Arabidopsis thaliana.

Abstract:

BACKGROUND:Vacuolar type H+-ATPases play a critical role in the maintenance of vacuolar homeostasis in plant cells. V-ATPases are also involved in plants' defense against environmental stress. This research examined the expression and regulation of the catalytic subunit of the vacuolar type H+-ATPase in Arabidopsis thaliana and the effect of environmental stress on multiple transcripts generated by this gene. RESULTS:Evidence suggests that subunit A of the vacuolar type H+-ATPase is encoded by a single gene in Arabidopsis thaliana. Genome blot analysis showed no indication of a second subunit A gene being present. The single gene identified was shown by whole RNA blot analysis to be transcribed in all organs of the plant. Subunit A was shown by sequencing the 3' end of multiple cDNA clones to exhibit multi site polyadenylation. Four different poly (A) tail attachment sites were revealed. Experiments were performed to determine the response of transcript levels for subunit A to environmental stress. A PCR based strategy was devised to amplify the four different transcripts from the subunit A gene. CONCLUSIONS:Amplification of cDNA generated from seedlings exposed to cold, salt stress, and etiolation showed that transcript levels for subunit A of the vacuolar type H+-ATPase in Arabidopsis were responsive to stress conditions. Cold and salt stress resulted in a 2-4 fold increase in all four subunit A transcripts evaluated. Etiolation resulted in a slight increase in transcript levels. All four transcripts appeared to behave identically with respect to stress conditions tested with no significant differential regulation.

journal_name

BMC Plant Biol

journal_title

BMC plant biology

authors

Magnotta SM,Gogarten JP

doi

10.1186/1471-2229-2-3

keywords:

subject

Has Abstract

pub_date

2002-04-02 00:00:00

pages

3

issn

1471-2229

journal_volume

2

pub_type

杂志文章
  • Drought stress in maize causes differential acclimation responses of glutathione and sulfur metabolism in leaves and roots.

    abstract:BACKGROUND:Drought is the most important environmental stress that limits crop yield in a global warming world. Despite the compelling evidence of an important role of oxidized and reduced sulfur-containing compounds during the response of plants to drought stress (e.g. sulfate for stomata closure or glutathione for sc...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-016-0940-z

    authors: Ahmad N,Malagoli M,Wirtz M,Hell R

    更新日期:2016-11-09 00:00:00

  • Identification of potential target genes for the tomato fruit-ripening regulator RIN by chromatin immunoprecipitation.

    abstract:BACKGROUND:During ripening, climacteric fruits increase their ethylene level and subsequently undergo various physiological changes, such as softening, pigmentation and development of aroma and flavor. These changes occur simultaneously and are caused by the highly synchronized expression of numerous genes at the onset...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-11-26

    authors: Fujisawa M,Nakano T,Ito Y

    更新日期:2011-01-30 00:00:00

  • The HaDREB2 transcription factor enhances basal thermotolerance and longevity of seeds through functional interaction with HaHSFA9.

    abstract:BACKGROUND:Transcription factor HaDREB2 was identified in sunflower (Helianthus annuus L.) as a drought-responsive element-binding factor 2 (DREB2) with unique properties. HaDREB2 and the sunflower Heat Shock Factor A9 (HaHSFA9) co-activated the Hahsp17.6G1 promoter in sunflower embryos. Both factors could be involved ...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-9-75

    authors: Almoguera C,Prieto-Dapena P,Díaz-Martín J,Espinosa JM,Carranco R,Jordano J

    更新日期:2009-06-19 00:00:00

  • Suppression of different classes of somatic mutations in Arabidopsis by vir gene-expressing Agrobacterium strains.

    abstract:BACKGROUND:Agrobacterium infection, which is widely used to generate transgenic plants, is often accompanied by T-DNA-linked mutations and transpositions in flowering plants. It is not known if Agrobacterium infection also affects the rates of point mutations, somatic homologous recombinations (SHR) and frame-shift mut...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-015-0595-1

    authors: Shah JM,Ramakrishnan AM,Singh AK,Ramachandran S,Unniyampurath U,Jayshankar A,Balasundaram N,Dhanapal S,Hyde G,Baskar R

    更新日期:2015-08-26 00:00:00

  • Boron-deficiency-responsive microRNAs and their targets in Citrus sinensis leaves.

    abstract:BACKGROUND:MicroRNAs play important roles in the adaptive responses of plants to nutrient deficiencies. Most research, however, has focused on nitrogen (N), phosphorus (P), sulfur (S), copper (Cu) and iron (Fe) deficiencies, limited data are available on the differential expression of miRNAs and their target genes in r...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-015-0642-y

    authors: Lu YB,Qi YP,Yang LT,Guo P,Li Y,Chen LS

    更新日期:2015-11-04 00:00:00

  • Functional analysis of the SlERF01 gene in disease resistance to S. lycopersici.

    abstract:BACKGROUND:Tomato gray leaf spot caused by Stemphylium lycopersici (S. lycopersici) is a serious disease that can severely hinder tomato production. To date, only Sm has been reported to provide resistance against this disease, and the molecular mechanism underlying resistance to this disease in tomato remains unclear....

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-02588-w

    authors: Yang H,Shen F,Wang H,Zhao T,Zhang H,Jiang J,Xu X,Li J

    更新日期:2020-08-15 00:00:00

  • Promotion of chloroplast proliferation upon enhanced post-mitotic cell expansion in leaves.

    abstract:BACKGROUND:Leaves are determinate organs; hence, precise control of cell proliferation and post-mitotic cell expansion is essential for their growth. A defect in cell proliferation often triggers enhanced post-mitotic cell expansion in leaves. This phenomenon is referred to as 'compensation'. Several lines of evidence ...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-13-143

    authors: Kawade K,Horiguchi G,Ishikawa N,Hirai MY,Tsukaya H

    更新日期:2013-09-28 00:00:00

  • Endophytic Penicillium funiculosum LHL06 secretes gibberellin that reprograms Glycine max L. growth during copper stress.

    abstract:BACKGROUND:Heavy metal pollution in crop fields is one of the major issues in sustainable agriculture production. To improve crop growth and reduce the toxic effects of metals is an ideal strategy. Understanding the resilience of gibberellins producing endophytic fungi associated with crop plants in metal contaminated ...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-13-86

    authors: Khan AL,Lee IJ

    更新日期:2013-05-31 00:00:00

  • B chromosome contains active genes and impacts the transcription of A chromosomes in maize (Zea mays L.).

    abstract:BACKGROUND:The dispensable maize (Zea mays L.) B chromosome is highly heterochromatic and widely believed to be devoid of functional genes. Although low-copy B chromosome causes no obvious phenotype variation, its existence might influence A genome gene expression. Previous studies suggested that B chromosomes are evol...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-016-0775-7

    authors: Huang W,Du Y,Zhao X,Jin W

    更新日期:2016-04-16 00:00:00

  • Protease gene families in Populus and Arabidopsis.

    abstract:BACKGROUND:Proteases play key roles in plants, maintaining strict protein quality control and degrading specific sets of proteins in response to diverse environmental and developmental stimuli. Similarities and differences between the proteases expressed in different species may give valuable insights into their physio...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-6-30

    authors: García-Lorenzo M,Sjödin A,Jansson S,Funk C

    更新日期:2006-12-20 00:00:00

  • Transcript and metabolite analysis in Trincadeira cultivar reveals novel information regarding the dynamics of grape ripening.

    abstract:BACKGROUND:Grapes (Vitis vinifera L.) are economically the most important fruit crop worldwide. However, the complexity of molecular and biochemical events that lead to the onset of ripening of nonclimacteric fruits is not fully understood which is further complicated in grapes due to seasonal and cultivar specific var...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-11-149

    authors: Fortes AM,Agudelo-Romero P,Silva MS,Ali K,Sousa L,Maltese F,Choi YH,Grimplet J,Martinez-Zapater JM,Verpoorte R,Pais MS

    更新日期:2011-11-02 00:00:00

  • Analysis of ripening-related gene expression in papaya using an Arabidopsis-based microarray.

    abstract:BACKGROUND:Papaya (Carica papaya L.) is a commercially important crop that produces climacteric fruits with a soft and sweet pulp that contain a wide range of health promoting phytochemicals. Despite its importance, little is known about transcriptional modifications during papaya fruit ripening and their control. In t...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-12-242

    authors: Fabi JP,Seymour GB,Graham NS,Broadley MR,May ST,Lajolo FM,Cordenunsi BR,Oliveira do Nascimento JR

    更新日期:2012-12-21 00:00:00

  • Genome-wide identification and evolutionary analysis of leucine-rich repeat receptor-like protein kinase genes in soybean.

    abstract:BACKGROUND:Leucine-rich repeat receptor-like kinases (LRR-RLKs) constitute the largest subfamily of receptor-like kinases in plant. A number of reports have demonstrated that plant LRR-RLKs play important roles in growth, development, differentiation, and stress responses. However, no comprehensive analysis of this gen...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-016-0744-1

    authors: Zhou F,Guo Y,Qiu LJ

    更新日期:2016-03-02 00:00:00

  • To be or not to be the odd one out--allele-specific transcription in pentaploid dogroses (Rosa L. sect. Caninae (DC.) Ser).

    abstract:BACKGROUND:Multiple hybridization events gave rise to pentaploid dogroses which can reproduce sexually despite their uneven ploidy level by the unique canina meiosis. Two homologous chromosome sets are involved in bivalent formation and are transmitted by the haploid pollen grains and the tetraploid egg cells. In addit...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-11-37

    authors: Ritz CM,Köhnen I,Groth M,Theissen G,Wissemann V

    更新日期:2011-02-23 00:00:00

  • Salinity tolerance, Na+ exclusion and allele mining of HKT1;5 in Oryza sativa and O. glaberrima: many sources, many genes, one mechanism?

    abstract:BACKGROUND:Cultivated rice species (Oryza sativa L. and O. glaberrima Steud.) are generally considered among the crop species most sensitive to salt stress. A handful of lines are known to be tolerant, and a small number of these have been used extensively as donors in breeding programs. However, these donors use many ...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-13-32

    authors: Platten JD,Egdane JA,Ismail AM

    更新日期:2013-02-27 00:00:00

  • Identification of plant leaf phosphorus content at different growth stages based on hyperspectral reflectance.

    abstract:BACKGROUND:Modern agriculture strives to sustainably manage fertilizer for both economic and environmental reasons. The monitoring of any nutritional (phosphorus, nitrogen, potassium) deficiency in growing plants is a challenge for precision farming technology. A study was carried out on three species of popular crops,...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-02807-4

    authors: Siedliska A,Baranowski P,Pastuszka-Woźniak J,Zubik M,Krzyszczak J

    更新日期:2021-01-07 00:00:00

  • Dynamics of gene expression associated with arsenic uptake and transport in rice during the whole growth period.

    abstract:BACKGROUND:Genes associated with arsenite uptake and transport in rice plants (i.e., OsLsi1, OsLsi2, OsLsi3, OsLsi6 and OsABCC1) have been identified to date. However, their expression over time during the whole growth period of rice under arsenite stress conditions is still poorly understood. In this study, the dynami...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-02343-1

    authors: Pan D,Yi J,Li F,Li X,Liu C,Wu W,Tao T

    更新日期:2020-03-31 00:00:00

  • Comparative metabolomics reveals the metabolic variations between two endangered Taxus species (T. fuana and T. yunnanensis) in the Himalayas.

    abstract:BACKGROUND:Plants of the genus Taxus have attracted much attention owing to the natural product taxol, a successful anti-cancer drug. T. fuana and T. yunnanensis are two endangered Taxus species mainly distributed in the Himalayas. In our study, an untargeted metabolomics approach integrated with a targeted UPLC-MS/MS ...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-018-1412-4

    authors: Yu C,Luo X,Zhan X,Hao J,Zhang L,L Song YB,Shen C,Dong M

    更新日期:2018-09-17 00:00:00

  • A generalized deceptive pollination system of Doritis pulcherrima (Aeridinae: Orchidaceae) with non-reconfigured pollinaria.

    abstract:BACKGROUND:As one of largest angiosperm families, orchids have long fascinated evolutionary biologists with their staggering diversity in floral design and display to promote outcrossing. Two of the most intriguing aspects of orchid pollination that promote cross-pollination are pollinarium reconfiguration (PR) and dec...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-12-67

    authors: Xiaohua J,Dezhu L,Zongxin R,Xiaoguo X

    更新日期:2012-07-03 00:00:00

  • A membrane-bound matrix-metalloproteinase from Nicotiana tabacum cv. BY-2 is induced by bacterial pathogens.

    abstract:BACKGROUND:Plant matrix metalloproteinases (MMP) are conserved proteolytic enzymes found in a wide range of monocotyledonous and dicotyledonous plant species. Acting on the plant extracellular matrix, they play crucial roles in many aspects of plant physiology including growth, development and the response to stresses ...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-9-83

    authors: Schiermeyer A,Hartenstein H,Mandal MK,Otte B,Wahner V,Schillberg S

    更新日期:2009-06-29 00:00:00

  • Rice hypersensitive induced reaction protein 1 (OsHIR1) associates with plasma membrane and triggers hypersensitive cell death.

    abstract:BACKGROUND:In plants, HIR (Hypersensitive Induced Reaction) proteins, members of the PID (Proliferation, Ion and Death) superfamily, have been shown to play a part in the development of spontaneous hypersensitive response lesions in leaves, in reaction to pathogen attacks. The levels of HIR proteins were shown to corre...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-10-290

    authors: Zhou L,Cheung MY,Li MW,Fu Y,Sun Z,Sun SM,Lam HM

    更新日期:2010-12-30 00:00:00

  • Plastomes of eight Ligusticum species: characterization, genome evolution, and phylogenetic relationships.

    abstract:BACKGROUND:The genus Ligusticum consists of approximately 60 species distributed in the Northern Hemisphere. It is one of the most taxonomically difficult taxa within Apiaceae, largely due to the varied morphological characteristics. To investigate the plastome evolution and phylogenetic relationships of Ligusticum, we...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-02696-7

    authors: Ren T,Li ZX,Xie DF,Gui LJ,Peng C,Wen J,He XJ

    更新日期:2020-11-13 00:00:00

  • Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance.

    abstract:BACKGROUND:The function of 4-coumarate-CoA ligases (4CL) under abiotic stresses has been studied in plants, however, limited is known about the 4CL genes in cotton (G. hirsutum L.) and their roles in response to drought stress. RESULTS:We performed genome-wide identification of the 4CL genes in G. hirsutum and investi...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-2329-2

    authors: Sun SC,Xiong XP,Zhang XL,Feng HJ,Zhu QH,Sun J,Li YJ

    更新日期:2020-03-23 00:00:00

  • Potential chromosomal introgression barriers revealed by linkage analysis in a hybrid of Pinus massoniana and P. hwangshanensis.

    abstract:BACKGROUND:Exploring the genetic mechanisms underlying speciation is a hot topic in modern genetics and evolutionary studies. Distortion of marker transmission ratio is frequently ascribed to selection against alleles that cause hybrid incompatibility. The natural introgression between P. massoniana and P. hwangshanens...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-10-37

    authors: Li S,Chen Y,Gao H,Yin T

    更新日期:2010-02-25 00:00:00

  • Characterization of a dominant mutation for the liguleless trait: Aegilops tauschii liguleless (Lgt).

    abstract:BACKGROUND:Leaves of Poaceae have a unique morphological feature: they consist of a proximal sheath and a distal blade separated by a ligular region. The sheath provides structural support and protects young developing leaves, whereas the main function of the blade is photosynthesis. The auricles allow the blade to til...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-019-1635-z

    authors: Dresvyannikova AE,Watanabe N,Muterko AF,Krasnikov AA,Goncharov NP,Dobrovolskaya OB

    更新日期:2019-02-15 00:00:00

  • Specific requirement for translation initiation factor 4E or its isoform drives plant host susceptibility to Tobacco etch virus.

    abstract:BACKGROUND:In plants, eIF4E translation initiation factors and their eIFiso4E isoforms are essential susceptibility factors for many RNA viruses, including potyviruses. Mutations altering these factors are a major source of resistance to the viruses. The eIF4E allelic series is associated with specific resistance spect...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-14-67

    authors: Estevan J,Maréna A,Callot C,Lacombe S,Moretti A,Caranta C,Gallois JL

    更新日期:2014-03-19 00:00:00

  • Arabidopsis NUCLEOSTEMIN-LIKE 1 (NSN1) regulates cell cycling potentially by cooperating with nucleosome assembly protein AtNAP1;1.

    abstract:BACKGROUND:In mammals, nucleostemin (NS), a nucleolar GTPase, is involved in stem cell proliferation, embryogenesis and ribosome biogenesis. Arabidopsis NUCLEOSTEMIN-LIKE 1 (NSN1) has previously been shown to be essential for plant growth and development. However, the role of NSN1 in cell proliferation is largely unkno...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-018-1289-2

    authors: Wang Z,Wang X,Xie B,Hong Z,Yang Q

    更新日期:2018-06-01 00:00:00

  • Symbiosis dependent accumulation of primary metabolites in arbuscule-containing cells.

    abstract:BACKGROUND:The arbuscular mycorrhizal symbiosis is characterized by the presence of different symbiotic structures and stages within a root system. Therefore tools allowing the analysis of molecular changes at a cellular level are required to reveal insight into arbuscular mycorrhizal (AM) symbiosis development and fun...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-015-0601-7

    authors: Gaude N,Bortfeld S,Erban A,Kopka J,Krajinski F

    更新日期:2015-09-30 00:00:00

  • De novo assembly and comparative analysis of the transcriptome of embryogenic callus formation in bread wheat (Triticum aestivum L.).

    abstract:BACKGROUND:During asexual reproduction the embryogenic callus can differentiate into a new plantlet, offering great potential for fostering in vitro culture efficiency in plants. The immature embryos (IMEs) of wheat (Triticum aestivum L.) are more easily able to generate embryogenic callus than mature embryos (MEs). To...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-017-1204-2

    authors: Chu Z,Chen J,Sun J,Dong Z,Yang X,Wang Y,Xu H,Zhang X,Chen F,Cui D

    更新日期:2017-12-19 00:00:00

  • High-resolution chromosome painting with repetitive and single-copy oligonucleotides in Arachis species identifies structural rearrangements and genome differentiation.

    abstract:BACKGROUND:Arachis contains 80 species that carry many beneficial genes that can be utilized in the genetic improvement of peanut (Arachis hypogaea L. 2n = 4x = 40, genome AABB). Chromosome engineering is a powerful technique by which these genes can be transferred and utilized in cultivated peanut. However, their smal...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-018-1468-1

    authors: Du P,Li L,Liu H,Fu L,Qin L,Zhang Z,Cui C,Sun Z,Han S,Xu J,Dai X,Huang B,Dong W,Tang F,Zhuang L,Han Y,Qi Z,Zhang X

    更新日期:2018-10-17 00:00:00