Abstract:
:Noninvasive methods are needed to study the kinetic properties of viruses in living organisms. Oncolytic viruses are used increasingly for cancer therapy but there is currently no satisfactory way to measure efficiency of tumor transduction, changing levels of viral gene expression or the timing of virus elimination. We therefore generated trackable oncolytic measles viruses expressing inert (nonimmunogenic, nonfunctional and accurately measurable) soluble marker peptides. The marker peptides did not compromise virus replication. Ex vivo and in vivo kinetics of the trackable viruses could be easily followed by measuring the concentrations of virally encoded marker peptides in culture supernatant or in serum. When mice bearing human tumor xenografts were challenged with the trackable viruses, distinct kinetic profiles of marker-gene expression could be correlated with distinct therapeutic outcomes. Oncolytic viruses expressing inert soluble marker polypeptides should greatly facilitate the rational development of effective, individually tailored cancer virotherapy.
journal_name
Nat Medjournal_title
Nature medicineauthors
Peng KW,Facteau S,Wegman T,O'Kane D,Russell SJdoi
10.1038/nm0502-527keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
527-31issue
5eissn
1078-8956issn
1546-170Xpii
nm0502-527journal_volume
8pub_type
杂志文章相关文献
NATURE MEDICINE文献大全abstract::The notoriously drug-resistant bug MRSA has made headlines for years, but a whole other class of bacteria may prove even more troublesome. These microbes, Gram-negative bacteria, are increasingly a threat--and yet not a single late-stage drug in development specifically targets them. Christian Torres follows one man's...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm0610-628
更新日期:2010-06-01 00:00:00
abstract::This corrects the article DOI: 10.1038/nm.4439. ...
journal_title:Nature medicine
pub_type: 已发布勘误
doi:10.1038/nm0418-526b
更新日期:2018-04-10 00:00:00
abstract::Endogenous mechanisms leading to host protection and resolution of infections without immunosuppression are of wide interest. Here we elucidate the structures of four new host-protective molecules produced in neutrophil-endothelial cocultures and present in human and mouse tissues after sterile inflammation or infecti...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm.3911
更新日期:2015-09-01 00:00:00
abstract::The World Health Organization estimates that tobacco will become the largest single health problem by 2020, causing an estimated 8.4 million deaths annually. But the smoking burden will not be distributed evenly across the globe; deaths in developed nations are set to rise 50% to 2.4 million while those in Asia will s...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/83281
更新日期:2001-01-01 00:00:00
abstract::To determine the protective potential of the humoral immune response against HIV-1 in vivo we evaluated the potency of three neutralizing antibodies (2G12, 2F5 and 4E10) in suppressing viral rebound in six acutely and eight chronically HIV-1-infected individuals undergoing interruption of antiretroviral treatment (ART...
journal_title:Nature medicine
pub_type: 临床试验,杂志文章
doi:10.1038/nm1244
更新日期:2005-06-01 00:00:00
abstract::Immunization of transgenic mouse models of Alzheimer disease using amyloid-beta peptide (Abeta) reduces both the Alzheimer disease-like neuropathology and the spatial memory impairments of these mice. However, a therapeutic trial of immunization with Abeta42 in humans was discontinued because a few patients developed ...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm790
更新日期:2002-11-01 00:00:00
abstract::Epstein-Barr virus is ubiquitous and is causally implicated in lymphoid and epithelial malignancies. Virus invades oropharyngeal mucosa and establishes latency in B lymphocytes. Reactivating lymphocytes shed virus into saliva for spread to new hosts. A complex of three virus glycoproteins, gH, gL and gp42, is essentia...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm0602-594
更新日期:2002-06-01 00:00:00
abstract::Treatment for schistosomiasis, which is responsible for more than 280,000 deaths annually, depends almost exclusively on praziquantel. Millions of people are treated annually with praziquantel, and drug-resistant parasites thus are likely to evolve. Phosphinic amides and oxadiazole 2-oxides, identified from a quantita...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm1737
更新日期:2008-04-01 00:00:00
abstract::A new study provides mechanistic insights into how live attenuated simian immunodeficiency virus (SIV) vaccines (LAVs) can protect monkeys from infection with pathogenic SIV. The authors show that replicating LAVs stimulate a protective immune response from a safe haven in the germinal centers of lymph nodes (pages 16...
journal_title:Nature medicine
pub_type: 评论,杂志文章
doi:10.1038/nm.2986
更新日期:2012-11-01 00:00:00
abstract::Conceptual and practical advances in molecular medicine are changing our understanding of cancer pathogenesis. In time this should provide the opportunity to alter the natural history of many cancers. ...
journal_title:Nature medicine
pub_type: 杂志文章,评审
doi:10.1038/nm0495-309
更新日期:1995-04-01 00:00:00
abstract::The use of genetically manipulated mouse models, gene and protein discovery and the cataloguing of genetic mutations have each allowed us to obtain new insights into skeletal morphogenesis and remodeling. These techniques have made it possible to identify molecules that are obligatory for specific cellular functions, ...
journal_title:Nature medicine
pub_type: 杂志文章,评审
doi:10.1038/nm1593
更新日期:2007-07-01 00:00:00
abstract::Solid organ transplants contain small numbers of leukocytes that can migrate into the host and establish long-lasting microchimerism. Although such microchimerism is often associated with graft acceptance and tolerance, it has been difficult to demonstrate a true causal link. Using skin from mutant mice deficient for ...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/83393
更新日期:2001-01-01 00:00:00
abstract::In chronic kidney disease (CKD), loss of functional nephrons results in metabolic and mechanical stress in the remaining ones, resulting in further nephron loss. Here we show that Akt2 activation has an essential role in podocyte protection after nephron reduction. Glomerulosclerosis and albuminuria were substantially...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm.3313
更新日期:2013-10-01 00:00:00
abstract::Cells that are deficient in homologous recombination, such as those that lack functional breast cancer-associated 1 (BRCA1) or BRCA2, are hypersensitive to inhibition of poly(ADP-ribose) polymerase (PARP). However, BRCA-deficient tumors represent only a small fraction of adult cancers, which might restrict the therape...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm.2377
更新日期:2011-06-26 00:00:00
abstract::The Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen (LANA) is expressed in all KSHV-associated tumors, including Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL). We found that beta-catenin is overexpressed in both PEL cells and KS tissue. Introduction of anti-LANA small inte...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm829
更新日期:2003-03-01 00:00:00
abstract::Metabolic reprograming is an emerging hallmark of tumor biology and an actively pursued opportunity in discovery of oncology drugs. Extensive efforts have focused on therapeutic targeting of glycolysis, whereas drugging mitochondrial oxidative phosphorylation (OXPHOS) has remained largely unexplored, partly owing to a...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/s41591-018-0052-4
更新日期:2018-07-01 00:00:00
abstract::High blood pressure, or hypertension, is a very common disorder with a substantial impact on public health because of its associated complications. Despite the high prevalence of essential hypertension and years of research, the basic causes remain obscure. Here I review recent advances in understanding the pathophysi...
journal_title:Nature medicine
pub_type: 杂志文章,评审
doi:10.1038/nm.2541
更新日期:2011-11-07 00:00:00
abstract::Brown adipose tissue (BAT) promotes a lean and healthy phenotype and improves insulin sensitivity. In response to cold or exercise, brown fat cells also emerge in the white adipose tissue (WAT; also known as beige cells), a process known as browning. Here we show that the development of functional beige fat in the ing...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm.3994
更新日期:2015-12-01 00:00:00
abstract::Direct evidence in humans for the impact of the microbiome on nutrient absorption is lacking. We conducted an extended inpatient study using two interventions that we hypothesized would alter the gut microbiome and nutrient absorption. In each, stool calorie loss, a direct proxy of nutrient absorption, was measured. T...
journal_title:Nature medicine
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41591-020-0801-z
更新日期:2020-04-01 00:00:00
abstract::HIV infection can cause extensive neuronal loss and clinically a severe dementia. The cause of the neurotoxicity remains unclear as neurons are not infected, but disturbance of glutamate-linked calcium entry has been implicated. In this study, we have shown a decrease in HIV-infected brain of the expression of mRNA an...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm1195-1174
更新日期:1995-11-01 00:00:00
abstract::Autosomal dominant polycystic kidney disease is an important cause of end-stage renal disease, for which there is no proven therapy. Mutations in PKD1 (the gene encoding polycystin-1) are the principal cause of this disease. The disease begins in utero and is slowly progressive, but it is not known whether cystogenesi...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm1675
更新日期:2007-12-01 00:00:00
abstract::Sphingosine-1-phosphate (S1P) signaling influences bone metabolism, but its therapeutic potential in bone disorders has remained unexplored. We show that raising S1P levels in adult mice through conditionally deleting or pharmacologically inhibiting S1P lyase, the sole enzyme responsible for irreversibly degrading S1P...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/s41591-018-0005-y
更新日期:2018-05-01 00:00:00
abstract::We previously demonstrated that low biosynthesis of ω-3 fatty acid-derived proresolution mediators, termed protectins, is associated with an impaired global resolution capacity, inflammation and insulin resistance in obese high-fat diet-fed mice. These findings prompted a more direct study of the therapeutic potential...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm.3549
更新日期:2014-06-01 00:00:00
abstract::LIGHT was recently described as a member of the tumor necrosis factor (TNF) 'superfamily'. We have isolated a mouse homolog of human LIGHT and investigated its immunoregulatory functions in vitro and in vivo. LIGHT has potent, CD28-independent co-stimulatory activity leading to T-cell growth and secretion of gamma int...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/73136
更新日期:2000-03-01 00:00:00
abstract::Secretion of gastric inhibitory polypeptide (GIP), a duodenal hormone, is primarily induced by absorption of ingested fat. Here we describe a novel pathway of obesity promotion via GIP. Wild-type mice fed a high-fat diet exhibited both hypersecretion of GIP and extreme visceral and subcutaneous fat deposition with ins...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm727
更新日期:2002-07-01 00:00:00
abstract::The severe disease atypical measles occurred when individuals immunized with a poorly protective inactivated vaccine contracted measles, and was postulated to be due to a lack of fusion-inhibiting antibodies. Here, rhesus macaques immunized with formalin-inactivated measles vaccine developed transient neutralizing and...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/9473
更新日期:1999-06-01 00:00:00
abstract::Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a potentially curative therapy for hematological malignancies. However, graft-versus-host disease (GVHD) and relapse after allo-HSCT remain major impediments to the success of allo-HSCT. Chimeric antigen receptors (CARs) direct tumor cell recognition of...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm.4258
更新日期:2017-02-01 00:00:00
abstract::Migration inhibitory factor (MIF) is known to exert significant pro-inflammatory effects and has the potential to override the anti-inflammatory action of glucocorticoids. In this study we have identified significant quantities of MIF in the alveolar airspaces of patients with acute respiratory distress syndrome (ARDS...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm0397-320
更新日期:1997-03-01 00:00:00
abstract:: ...
journal_title:Nature medicine
pub_type: 评论,杂志文章
doi:10.1038/s41591-018-0286-1
更新日期:2018-12-01 00:00:00
abstract::Members of the suppressor of cytokine signaling (SOCS) family are involved in the pathogenesis of many inflammatory diseases. SOCS-3 is predominantly expressed in T-helper type 2 (T(H)2) cells, but its role in T(H)2-related allergic diseases remains to be investigated. In this study we provide a strong correlation bet...
journal_title:Nature medicine
pub_type: 杂志文章
doi:10.1038/nm896
更新日期:2003-08-01 00:00:00