Identification in traditional herbal medications and confirmation by synthesis of factors that inhibit cholera toxin-induced fluid accumulation.

Abstract:

:Kampo formulations are traditional herbal medications used in China and Japan for many centuries to treat diarrheal diseases such as cholera. Our studies were undertaken to identify and verify by chemical synthesis the active components that inhibited cholera toxin (CT), the virulence factor secreted by Vibrio cholerae, the causative agent of cholera. The Kampo formulation, Daio-kanzo-to, inhibited CT activities (i.e., ADP-ribosylation, Chinese hamster ovary cell elongation); in Daio-kanzo-to, Daio (Rhei rhizoma) was responsible for this effect. Among several components purified from Daio extract, rhubarb galloyl-tannin, a compound characterized by a polygallate structure, was the most effective. To define the active component, gallate analogues similar to rhubarb galloyl-tannin were synthesized. These gallate compounds inhibited all CT activities including ADP-ribosylation, elongation of Chinese hamster ovary cells, and importantly, fluid accumulation in ileal loops. Thus, Kampo formulations or their gallate components might be effective adjunctive therapy with oral rehydration solution for the severe diarrhea of cholera.

authors

Oi H,Matsuura D,Miyake M,Ueno M,Takai I,Yamamoto T,Kubo M,Moss J,Noda M

doi

10.1073/pnas.052709499

keywords:

subject

Has Abstract

pub_date

2002-03-05 00:00:00

pages

3042-6

issue

5

eissn

0027-8424

issn

1091-6490

pii

052709499

journal_volume

99

pub_type

杂志文章
  • Single d(ApG)/cis-diamminedichloroplatinum(II) adduct-induced mutagenesis in Escherichia coli.

    abstract::The mutation spectrum induced by the widely used antitumor drug cis-diamminedichloroplatinum(II) (cis-DDP) showed that cisDDP[d(ApG)] adducts, although they account for only 25% of the lesions formed, are approximately 5 times more mutagenic than the major GG adduct. We report the construction of vectors bearing a sin...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.16.6087

    authors: Burnouf D,Gauthier C,Chottard JC,Fuchs RP

    更新日期:1990-08-01 00:00:00

  • Lipocalin-type prostaglandin D synthase/beta-trace is a major amyloid beta-chaperone in human cerebrospinal fluid.

    abstract::The conformational change in amyloid beta (Abeta) peptide from its monomeric form to aggregates is crucial in the pathogenesis of Alzheimer's disease (AD). In the healthy brain, some unidentified chaperones appear to prevent the aggregation of Abeta. Here we reported that lipocalin-type prostaglandin D synthase (L-PGD...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0701585104

    authors: Kanekiyo T,Ban T,Aritake K,Huang ZL,Qu WM,Okazaki I,Mohri I,Murayama S,Ozono K,Taniike M,Goto Y,Urade Y

    更新日期:2007-04-10 00:00:00

  • The Arabidopsis SOS2 protein kinase physically interacts with and is activated by the calcium-binding protein SOS3.

    abstract::The Arabidopsis thaliana SOS2 and SOS3 genes are required for intracellular Na(+) and K(+) homeostasis and plant tolerance to high Na(+) and low K(+) environments. SOS3 is an EF hand type calcium-binding protein having sequence similarities with animal neuronal calcium sensors and the yeast calcineurin B. SOS2 is a se...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.040577697

    authors: Halfter U,Ishitani M,Zhu JK

    更新日期:2000-03-28 00:00:00

  • Lysine methyltransferase G9a methylates the transcription factor MyoD and regulates skeletal muscle differentiation.

    abstract::Skeletal muscle cells have served as a paradigm for understanding mechanisms leading to cellular differentiation. The proliferation and differentiation of muscle precursor cells require the concerted activity of myogenic regulatory factors including MyoD. In addition, chromatin modifiers mediate dynamic modifications ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1111628109

    authors: Ling BM,Bharathy N,Chung TK,Kok WK,Li S,Tan YH,Rao VK,Gopinadhan S,Sartorelli V,Walsh MJ,Taneja R

    更新日期:2012-01-17 00:00:00

  • Germ-line expression of an oncogenic erbB2 allele confers resistance to erbB2-induced mammary tumorigenesis.

    abstract::We have previously shown that mammary epithelial specific expression of the activated erbB2 allele under the control of the endogenous promoter in mice resulted in the formation of mammary adenocarcinomas. To assess whether mammary tumorigenesis in this model is influenced by the developmental window of expression, we...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0306802101

    authors: Andrechek ER,Hardy WR,Laing MA,Muller WJ

    更新日期:2004-04-06 00:00:00

  • Ras-independent transformation by v-Src.

    abstract::Signaling by a variety of receptor and nonreceptor tyrosine kinases is mediated by Ras, a membrane-associated GTPase. Expression of v-Src, a transforming nonreceptor tyrosine kinase, results in Ras activation, and inhibition of Ras function in NIH 3T3 cells suppresses transformation by v-Src, indicating that in these ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.7.3028

    authors: Aftab DT,Kwan J,Martin GS

    更新日期:1997-04-01 00:00:00

  • Anomalous anisotropic compression behavior of superconducting CrAs under high pressure.

    abstract::CrAs was observed to possess the bulk superconductivity under high-pressure conditions. To understand the superconducting mechanism and explore the correlation between the structure and superconductivity, the high-pressure structural evolution of CrAs was investigated using the angle-dispersive X-ray diffraction (XRD)...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1520570112

    authors: Yu Z,Wu W,Hu Q,Zhao J,Li C,Yang K,Cheng J,Luo J,Wang L,Mao HK

    更新日期:2015-12-01 00:00:00

  • A de novo missense mutation of the beta subunit of the epithelial sodium channel causes hypertension and Liddle syndrome, identifying a proline-rich segment critical for regulation of channel activity.

    abstract::Liddle syndrome is a mendelian form of hypertension characterized by constitutively elevated renal Na reabsorption that can result from activating mutations in the beta or gamma subunit of the epithelial Na channel. All reported mutations have deleted the last 45-76 normal amino acids from the cytoplasmic C terminus o...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.25.11495

    authors: Hansson JH,Schild L,Lu Y,Wilson TA,Gautschi I,Shimkets R,Nelson-Williams C,Rossier BC,Lifton RP

    更新日期:1995-12-05 00:00:00

  • Chromosome 14 transfer and functional studies identify a candidate tumor suppressor gene, mirror image polydactyly 1, in nasopharyngeal carcinoma.

    abstract::Chromosome 14 allelic loss is common in nasopharyngeal carcinoma (NPC) and may reflect essential tumor suppressor gene loss in tumorigenesis. An intact chromosome 14 was transferred to an NPC cell line using a microcell-mediated chromosome transfer approach. Microcell hybrids (MCHs) containing intact exogenously trans...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0900198106

    authors: Cheung AK,Lung HL,Ko JM,Cheng Y,Stanbridge EJ,Zabarovsky ER,Nicholls JM,Chua D,Tsao SW,Guan XY,Lung ML

    更新日期:2009-08-25 00:00:00

  • Clues to the mechanism of cholesterol transfer from the structure of NPC1 middle lumenal domain bound to NPC2.

    abstract::Export of LDL-derived cholesterol from lysosomes requires the cooperation of the integral membrane protein Niemann-Pick C1 (NPC1) and a soluble protein, Niemann-Pick C2 (NPC2). Mutations in the genes encoding these proteins lead to Niemann-Pick disease type C (NPC). NPC2 binds to NPC1's second (middle), lumenally orie...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1611956113

    authors: Li X,Saha P,Li J,Blobel G,Pfeffer SR

    更新日期:2016-09-06 00:00:00

  • Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells.

    abstract::The intracellular activation of trypsinogen, which is both pH- and calcium-dependent, is an important early step in the development of acute pancreatitis. The cellular compartment in which trypsinogen activation occurs currently is unknown. We therefore investigated the site of intracellular trypsinogen activation by ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0700951104

    authors: Sherwood MW,Prior IA,Voronina SG,Barrow SL,Woodsmith JD,Gerasimenko OV,Petersen OH,Tepikin AV

    更新日期:2007-03-27 00:00:00

  • Amitochondriate amoebae and the evolution of DNA-dependent RNA polymerase II.

    abstract::Unlike parasitic protist groups that are defined by the absence of mitochondria, the Pelobiontida is composed mostly of free-living species. Because of the presence of ultrastructural and cellular features that set them apart from all other eukaryotic organisms, it has been suggested that pelobionts are primitively am...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.20.11769

    authors: Stiller JW,Duffield EC,Hall BD

    更新日期:1998-09-29 00:00:00

  • Isolation of a high molecular weight actin-binding protein from baby hamster kidney (BHK-21) cells.

    abstract::A high molecular weight protein (HMWP) with properties similar to those of both actin-binding protein (ABP) and filamin has been isolated from cultured baby hamster kidney (BHK-21) cells. The protein was present in an actomyosin-depleted sucrose extract of the cells and was eluted, upon gel chromatography on Sepharose...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.9.4484

    authors: Schloss JA,Goldman RD

    更新日期:1979-09-01 00:00:00

  • Water does not flow across the tight junctions of MDCK cell epithelium.

    abstract::Although it has been known for decades that the tight junctions of fluid-transporting epithelia are leaky to ions, it has not been possible to determine directly whether significant transjunctional water movement also occurs. An optical microscopic technique was developed for the direct visualization of the flow veloc...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.11.6526

    authors: Kovbasnjuk O,Leader JP,Weinstein AM,Spring KR

    更新日期:1998-05-26 00:00:00

  • Developmental processes underlying the evolution of a derived foot morphology in salamanders.

    abstract::Interdigital webbing has evolved repeatedly in tropical salamanders (bolitoglossines). This derived foot morphology is only one of many homoplastic traits in this diverse amphibian clade. Indeed, few if any morphological traits sort lineages within this clade. We investigate the processes underlying the homoplastic ev...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0710216105

    authors: Jaekel M,Wake DB

    更新日期:2007-12-18 00:00:00

  • Subunit functional studies of NAD(P)H:quinone oxidoreductase with a heterodimer approach.

    abstract::NAD(P)H:quinone oxidoreductase (NQOR; EC 1.6.99.2) is a homodimeric enzyme which catalyzes the reduction of quinones, azo dyes, and other electron acceptors by NADPH or NADH. To pursue subunit functional studies, we expressed a wild-type/mutant heterodimer of NQOR in Escherichia coli. The wild-type subunit of the hete...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.4.1043

    authors: Cui K,Lu AY,Yang CS

    更新日期:1995-02-14 00:00:00

  • Differences between high-affinity forskolin binding sites in dopamine-rich and other regions of rat brain.

    abstract::[3H]Forskolin bound to high- and low-affinity sites in the rat brain. The high-affinity site was discretely located, with highest densities in the striatum, nucleus accumbens, olfactory tubercule, substantia nigra, hippocampus, and the molecular layers of the cerebellum. This site did not correlate well with the distr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.9.3216

    authors: Poat JA,Cripps HE,Iversen LL

    更新日期:1988-05-01 00:00:00

  • Nanoscale spatial organization of the HoxD gene cluster in distinct transcriptional states.

    abstract::Chromatin condensation plays an important role in the regulation of gene expression. Recently, it was shown that the transcriptional activation of Hoxd genes during vertebrate digit development involves modifications in 3D interactions within and around the HoxD gene cluster. This reorganization follows a global trans...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1517972112

    authors: Fabre PJ,Benke A,Joye E,Nguyen Huynh TH,Manley S,Duboule D

    更新日期:2015-11-10 00:00:00

  • Force measurements on cargoes in living cells reveal collective dynamics of microtubule motors.

    abstract::Many cellular cargoes move bidirectionally along microtubules, driven by teams of plus- and minus-end-directed motor proteins. To probe the forces exerted on cargoes during intracellular transport, we examined latex beads phagocytosed into living mammalian macrophages. These latex bead compartments (LBCs) are encased ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1215462109

    authors: Hendricks AG,Holzbaur EL,Goldman YE

    更新日期:2012-11-06 00:00:00

  • The regulation of DNA synthesis in quiescent lymphocytes by cytoplasmic inhibitors.

    abstract::We previously have shown that proliferating lymphoid cells contain an extractable cytoplasmic protein that is capable of inducing DNA synthesis in isolated quiescent nuclei (activator of DNA replication, ADR). ADR is present in continuously proliferating (transformed) lymphoblastoid cell lines and in mitogen- or inter...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.15.5160

    authors: Gutowski JK,West A,Cohen S

    更新日期:1985-08-01 00:00:00

  • Chloroplast DNA inversions and the origin of the grass family (Poaceae).

    abstract::The phylogenetic affinities of the grass family (Poaceae) have long been debated. The chloroplast genomes of at least some grasses have been known to possess three inversions relative to the typical gene arrangement found in most flowering plants. We have surveyed for the presence of these inversions in grasses and ot...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.16.7722

    authors: Doyle JJ,Davis JI,Soreng RJ,Garvin D,Anderson MJ

    更新日期:1992-08-15 00:00:00

  • Predicting coding function from nucleotide sequence or survival of "fitness" of tRNA.

    abstract::The sequence of a nucleotide region of f1 bacteriophage was determined on a bonded ultrathin acrylamide gel with a discontinuous buffer system by using the dideoxy-DNA sequencing method. This sequence and one other were analyzed for maximal base pairing with tRNAs. The results allow a prediction of the direction and p...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.77.6.3539

    authors: Pieczenik G

    更新日期:1980-06-01 00:00:00

  • High-density lipoprotein receptor SCARB1 is required for carotenoid coloration in birds.

    abstract::Yellow, orange, and red coloration is a fundamental aspect of avian diversity and serves as an important signal in mate choice and aggressive interactions. This coloration is often produced through the deposition of diet-derived carotenoid pigments, yet the mechanisms of carotenoid uptake and transport are not well-un...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1700751114

    authors: Toomey MB,Lopes RJ,Araújo PM,Johnson JD,Gazda MA,Afonso S,Mota PG,Koch RE,Hill GE,Corbo JC,Carneiro M

    更新日期:2017-05-16 00:00:00

  • The fossil record of phenotypic integration and modularity: A deep-time perspective on developmental and evolutionary dynamics.

    abstract::Variation is the raw material for natural selection, but the factors shaping variation are still poorly understood. Genetic and developmental interactions can direct variation, but there has been little synthesis of these effects with the extrinsic factors that can shape biodiversity over large scales. The study of ph...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1403667112

    authors: Goswami A,Binder WJ,Meachen J,O'Keefe FR

    更新日期:2015-04-21 00:00:00

  • Large electrical currents traverse developing Ceropia follicles.

    abstract::An intense (up to 20 muA/cm(2)) steady electrical current enters the anterior or nurse cell end of the growing follicle (or oocyte-nurse cell complex) of the Cecropia moth and is balanced by a more diffuse current leaving elsewhere. In late growth stages, the total transfollicular current is about 100 nA. Moreover, a ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.3.1328

    authors: Jaffe LF,Woodruff RI

    更新日期:1979-03-01 00:00:00

  • Nonhuman primate event-related potentials indexing covert shifts of attention.

    abstract::A half-century's worth of research has established the existence of numerous event-related potential components measuring different cognitive operations in humans including the selection of stimuli by covert attention mechanisms. Surprisingly, it is unknown whether nonhuman primates exhibit homologous electrophysiolog...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0703477104

    authors: Woodman GF,Kang MS,Rossi AF,Schall JD

    更新日期:2007-09-18 00:00:00

  • Radiation dose rates now and in the future for residents neighboring restricted areas of the Fukushima Daiichi Nuclear Power Plant.

    abstract::Radiation dose rates were evaluated in three areas neighboring a restricted area within a 20- to 50-km radius of the Fukushima Daiichi Nuclear Power Plant in August-September 2012 and projected to 2022 and 2062. Study participants wore personal dosimeters measuring external dose equivalents, almost entirely from depos...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1315684111

    authors: Harada KH,Niisoe T,Imanaka M,Takahashi T,Amako K,Fujii Y,Kanameishi M,Ohse K,Nakai Y,Nishikawa T,Saito Y,Sakamoto H,Ueyama K,Hisaki K,Ohara E,Inoue T,Yamamoto K,Matsuoka Y,Ohata H,Toshima K,Okada A,Sato H,Kuwa

    更新日期:2014-03-11 00:00:00

  • Oral immunization and protection of raccoons (Procyon lotor) with a vaccinia-rabies glycoprotein recombinant virus vaccine.

    abstract::Animal rabies control has been frustrated by the existence of multiple wildlife reservoirs and the lack of efficacious oral vaccines. In this investigation, raccoons fed a vaccinia-rabies glycoprotein recombinant virus in a sponge bait developed rabies virus-neutralizing antibody (0.6-54.0 units) and resisted street r...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.20.7947

    authors: Rupprecht CE,Wiktor TJ,Johnston DH,Hamir AN,Dietzschold B,Wunner WH,Glickman LT,Koprowski H

    更新日期:1986-10-01 00:00:00

  • Oxymetry deep in tissues with low-frequency electron paramagnetic resonance.

    abstract::We have measured the oxygen concentration in the body water of murine FSa and NFSa fibrosarcomas using a new method for quantitative oxygen concentration determination deep in the tissues of a living animal. The measurement uses unusually low-frequency electron paramagnetic spectroscopy sensitive to substrate 7 cm dee...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.26.13047

    authors: Halpern HJ,Yu C,Peric M,Barth E,Grdina DJ,Teicher BA

    更新日期:1994-12-20 00:00:00

  • Metal-induced sensor mobilization turns on affinity to activate regulator for metal detoxification in live bacteria.

    abstract::Metal detoxification is essential for bacteria's survival in adverse environments and their pathogenesis in hosts. Understanding the underlying mechanisms is crucial for devising antibacterial treatments. In the Gram-negative bacterium Escherichia coli, membrane-bound sensor CusS and its response regulator CusR togeth...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1919816117

    authors: Fu B,Sengupta K,Genova LA,Santiago AG,Jung W,Krzemiński Ł,Chakraborty UK,Zhang W,Chen P

    更新日期:2020-06-16 00:00:00