Abstract:
:Kampo formulations are traditional herbal medications used in China and Japan for many centuries to treat diarrheal diseases such as cholera. Our studies were undertaken to identify and verify by chemical synthesis the active components that inhibited cholera toxin (CT), the virulence factor secreted by Vibrio cholerae, the causative agent of cholera. The Kampo formulation, Daio-kanzo-to, inhibited CT activities (i.e., ADP-ribosylation, Chinese hamster ovary cell elongation); in Daio-kanzo-to, Daio (Rhei rhizoma) was responsible for this effect. Among several components purified from Daio extract, rhubarb galloyl-tannin, a compound characterized by a polygallate structure, was the most effective. To define the active component, gallate analogues similar to rhubarb galloyl-tannin were synthesized. These gallate compounds inhibited all CT activities including ADP-ribosylation, elongation of Chinese hamster ovary cells, and importantly, fluid accumulation in ileal loops. Thus, Kampo formulations or their gallate components might be effective adjunctive therapy with oral rehydration solution for the severe diarrhea of cholera.
journal_name
Proc Natl Acad Sci U S Aauthors
Oi H,Matsuura D,Miyake M,Ueno M,Takai I,Yamamoto T,Kubo M,Moss J,Noda Mdoi
10.1073/pnas.052709499keywords:
subject
Has Abstractpub_date
2002-03-05 00:00:00pages
3042-6issue
5eissn
0027-8424issn
1091-6490pii
052709499journal_volume
99pub_type
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