Abstract：
OBJECTIVE:The aim of this study was to characterize patient antibodies before and after cadaver and/or living-donor kidney transplantation and to correlate these data with the clinical course after transplantation. METHODS:Sera from 69 cadaver, 9 living-related and 2 patients waiting for living-donor kidney transplantation were analyzed by the complement dependent cytotoxicity (CDC) test, flow cytometry (FCXM) and ELISA. RESULTS:FCXM revealed that 15.0% of patients before transplantation and 16.7% after transplantation had antibodies to donor cells. 10.3% patients were positive before and after transplantation (+/+), while 6.8% developed antibodies early after transplantation (-/+). Analysis of the specificity of those antibodies by ELISA showed that it was directed to: 1) mismatched donor HLA antigens 2) antigens belonging to the same cross-reacting group (CREG) as the mismatched donor antigens 3) HLA antigens not expressed by donor cells or, probably, to non-HLA antigens. CONCLUSION:Anti-HLA antibodies were detected in patients before and after transplantation and in most cases their anti-HLA specificity could be determined. Fast and precise characterization of antibodies in patients before and after transplantation can be performed by both sensitive methods--FCXM and ELISA--which may help predict the onset of immunological complications and, consequently, improve the prognosis after organ transplantation.
journal_name
Ann Transplantjournal_title
Annals of transplantationauthors
Slavcev A,Lácha J,Sajdlová H,Vítko S,Horcicková M,Stríz I,Ivasková Ekeywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
12-5issue
2eissn
1425-9524issn
2329-0358journal_volume
6pub_type
杂志文章abstract：BACKGROUND:Institut Georges Lopez-1 (IGL-1) is a new preservation solution with lower potassium and lower viscosity than University Wisconsin solution (UW). These characteristics which improve liver preservation lead us to evaluate clinical effects of IGL-1 in a randomized controlled study with UW. MATERIAL/METHODS:Fr...
journal_title：Annals of transplantation
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2010-10-01 00:00:00
abstract：BACKGROUND:Despite documented positive effect of MachinePerfusion (MP) on long-term kidneys-graft function its wide use is restricted due to higher costs. The aim of this study was to analyze the difference in costs of kidney transplantation in patients who received organ stored in ColdStorage (CS) vs those who receive...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：2009-04-01 00:00:00
abstract：BACKGROUND:Studies have documented deficits of pneumococcal and other vaccinations in kidney and lung transplant patients, but the vaccination status of liver transplant (LT) recipients is unknown. This cross-sectional study investigated the prevalence of immunizations among LT patients at a large university medical ce...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/aot.896436
更新日期：2016-04-07 00:00:00
abstract：UNLABELLED:In spite of progress in biotechnology, isolation of porcine pancreatic islets remains a difficult task with unpredictable results. One reason could be the lack of knowledge as to the expression of extracellular matrix proteins in porcine exocrine and endocrine tissues, particularly in "islet capsules". Such ...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:The patient is a 28 year old woman who received a heart transplant in 1992 secondary to hypertrophic cardiomyopathy with unremarkable post-operative course. In the period immediately post transplantation the patient was on a four-drug immunosuppressive regimen which was subsequently changed to standard three-agent the...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：BACKGROUND:Transplant renal artery stenosis (TRAS) is a serious vascular complication due to non-specific clinical manifestations, causing serious diagnostic difficulties. Contrast-enhanced ultrasound (CE-US) can complement standard sonographic examination in evaluation of TRAS. MATERIAL AND METHODS:Standard ultrasoun...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/AOT.883896
更新日期：2013-04-26 00:00:00
abstract：:BACKGROUND The aim of this study was to investigate the clinical features and treatment strategies of transplant renal artery stenosis (TRAS) with kidneys from donation after cardiac death (DCD). MATERIAL AND METHODS We collected the clinical data of donors and recipients of single-center DCD-induced TRAS from January...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/AOT.918076
更新日期：2020-02-04 00:00:00
abstract：BACKGROUND:Achievement of sustained virological response (SVR) is the main goal of interferon/ribavirin (IFN/RBV) antiviral therapy in patients with hepatitis C virus (HCV) recurrence after orthotopic liver transplantation (OLT). In this study, we have retrospectively evaluated the efficacy and safety of low accelerati...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/AOT.892255
更新日期：2015-05-11 00:00:00
abstract：:BACKGROUND Hyperuricemia is associated with an increased risk of cardiovascular disease and chronic allograft nephropathy after renal transplantation. It has recently been demonstrated that treatment of asymptomatic hyperuricemia is associated with improved patient and graft survival; although, the underlying mechanis...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/aot.901657
更新日期：2017-07-14 00:00:00
abstract：:Over the last decade significant improvement in patient and graft survival has been observed after liver transplantation for hepatitis B virus (HBV)-related liver disease, mostly because of efficient prophylaxis against hepatitis B reinfection. This review discusses different approches in prevention of hepatitis B rec...
journal_title：Annals of transplantation
pub_type： 杂志文章,评审
doi：
更新日期：2005-01-01 00:00:00
abstract：:Recognition of mismatched (foreign) alloantigens on the graft by the recipients T cells initiates allograft rejection. This process is mediated by two mayor mechanisms--direct and indirect allorecognition. Both process are being discussed. ...
journal_title：Annals of transplantation
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
abstract：:BACKGROUND Percutaneous liver biopsy is an established diagnostic procedure for the assessment of liver pathologies. Limited data are available on the clinical impact of liver biopsies in liver transplant recipients. MATERIAL AND METHODS Liver transplant recipients undergoing liver biopsy between 2000 and 2013 were an...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/aot.901487
更新日期：2017-02-24 00:00:00
abstract：:Transplantation of liver has been remarkably effective in the treatment of liver failure and liver-based inherited metabolic diseases and has revolutionized the care of patients with end-stage liver disease. Unfortunately demand for transplantable livers is progressively outpacing the supply of donated cadaver organs,...
journal_title：Annals of transplantation
pub_type： 杂志文章,评审
doi：
更新日期：2007-01-01 00:00:00
abstract：UNLABELLED:Glutathione S-Tranferases (GST) are the enzymes which are strictly specific for epithelial cells of the proximal and distal tubules in the kidney. These enzymes are detected in the urine when some tubular damage process is found. In healthy people urine GST is hardly detected. The goal of this study was to e...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The liver immune function is associated with specific lymphocyte population transiently marginated in the liver sinusoids. These cells are of blood origin, however they are phenotypically and functionally different from peripheral blood lymphocytes. The question arises whether tumor proliferating in the liver can modi...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:102 children have been treated at the Institute of Traumatology, Orthopaedics & Neurosurgery a result of scoliosis. In all of these multi-step treatment has been applied. Initially a telescopic rod was implanted into the spine. Allografts were introduced around the rod hook, after 6 or 8 months, when angle of scoliosi...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The number of liver grafts obtained from a cadaver can be greatly increased with the application of split liver transplantation. In the last 10 years, pediatric waiting list mortality has been reduced significantly with the use of this form of liver transplantation, which has 2 major forms. In its most commonly used f...
journal_title：Annals of transplantation
pub_type： 杂志文章,评审
doi：10.12659/aot.896351
更新日期：2016-07-01 00:00:00
abstract：BACKGROUND:As newer immunosuppressive regimens have steadily reduced in the incidence of acute rejection and have extended the life expectancy of allograft recipients, posttransplant de novo malignancies have become an important cause of death in cadaveric donor transplantation. Also, according with the recent accumula...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/aot.883230
更新日期：2012-04-01 00:00:00
abstract：:BACKGROUND Laparoscopic donor nephrectomy (LDN) has evolved and has been established as a surgical standard of care for kidney transplantation. MATERIAL AND METHODS This study retrospectively reviews 1132 patients who underwent 4 different laparoscopic living-donor nephrectomies: hand-assisted laparoscopic nephrectomy...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/AOT.918189
更新日期：2020-02-11 00:00:00
abstract：:The use of xenografts could relieve the chronically inadequate supply of human organs for transplantation, but doubts have been expressed about the general acceptability of transplanting animal organs into human. Some researchers and clinicians have chosen to ignore negative attitudes towards clinical xenotransplantat...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：BACKGROUND:Autologous peripheral blood stem cell transplantation (PBSCT) is commonly used in the treatment of lymphoma patients. G-CSF is widely used to boost white blood cell recovery. However, there are no clear data indicating which strategy of using G-CSF provides the most benefit. The aim of our study was to compa...
journal_title：Annals of transplantation
pub_type： 杂志文章,多中心研究
doi：10.12659/AOT.883965
更新日期：2013-07-02 00:00:00
abstract：:Applications of allogenic amnion grafts range from wound dressing of severe burns, dermabrasions and lower extremity ulcer treatments to plastic surgery, laryngology and ophthalmology. The aim of the present study was to elaborate the method of processing, preservation and sterilization of human amnion allografts prep...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:BACKGROUND Acute graft-versus-host disease (aGVHD) remains the most common and challenging complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). An important obstacle to the therapeutic effect of aGVHD is the inability to identify risk factors for an individual patient at the onset of symp...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/aot.901495
更新日期：2017-02-03 00:00:00
abstract：BACKGROUND:Xenotransplantation from pig to humans may be associated with the risk of transmission of porcine endogenous retroviruses (PERVs) that are present in the genome of all pigs and that infect human cells in vitro. However, it remains unclear whether PERVs infect transplant recipients in vivo and, if so, whether...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Recovery of the patients after transplantation of non-renal organs may be complicated by multi-faceted chronic renal failure (CKD) which is regarded as an independent risk factor of graft dysfunction and mortality. The occurrence of CKD in non-renal transplant recipients depends mainly on a type of transplanted organ,...
journal_title：Annals of transplantation
pub_type： 杂志文章,评审
doi：
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:The activation of the tryptophane catabolizing enzyme Indoleamine 2,3-dioxygenase leads to the formation of kynurenine and other metabolites that counter-regulate immune activation resulting in restoration of immune homeostasis. But in chronic immune activation, as in hemodialysed patients, the immunosuppres...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/AOT.889764
更新日期：2014-01-27 00:00:00
abstract：:BACKGROUND Solid organ transplantations lead to improvements in patient survival and patient quality of life, as well as health care system economic benefits. However, over time, health problems can accumulate post-transplantation. Therefore, we hypothesized that in the late post-transplantation period, the costs of p...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/AOT.914661
更新日期：2019-05-07 00:00:00
abstract：:For each individual, there is a limit to the capacity to perform exercise. The limitation, however, depends on the nature of the task and is also influenced by a number of other factors. Muscle strength is determined largely by muscle mass, specifically muscle cross-sectional area, but is also influenced by neural dri...
journal_title：Annals of transplantation
pub_type： 杂志文章,评审
doi：
更新日期：2005-01-01 00:00:00
abstract：:Human BK polyomavirus (BKV) infection is poorly documented in heart and lung transplant patients. BK viruria and viremia have been estimated to affect 19% and 5% of heart transplant recipients, respectively. Data are limited, especially for lung transplantation, but the proportion of patients progressing from BK virur...
journal_title：Annals of transplantation
pub_type： 杂志文章,评审
doi：10.12659/AOT.908429
更新日期：2018-05-11 00:00:00
abstract：:BACKGROUND In kidney transplantation, the association of cold ischemia time (CIT), anastomosis time (AT), and delayed graft function (DGF) is particularly detrimental in grafts from marginal donors; however, actual cut-off criteria are still debated. MATERIAL AND METHODS Data from patients >65 years (n=193) and patien...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/aot.896672
更新日期：2016-05-31 00:00:00
