Abstract：
OBJECTIVE:To study the interaction between oncogene mdm2 and wp53 in human glandular lung cancer cell line GLC-82. METHODS:By lipofectamine-mediated DNA transfection, wp53 and mdm2 were transfected separately or co-transfected into GLC-82 cells via retrovival vector pDOR-neo, a carrier of wp53 and mdm2. RESULTS:The growth of GLC-82 cells was blocked and their DNA synthesis inhibited by wp53, its colony-forming rate in soft agar culture and the tumorigenicity in nude mice declined and mdm2 antagonized the function of wp53. CONCLUSION:After the recombinant vector pDOR-mdm2 was transfected into GLC-82 cells containing wp53, mdm2 partially deprives wp53 of its function of inhibiting the growth of GLC-82 cells.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Li P,Rao G,Chen Y,Ding Z,Yu Wkeywords:
subject
Has Abstractpub_date
1999-09-01 00:00:00pages
850-3issue
9eissn
0366-6999issn
2542-5641journal_volume
112pub_type
杂志文章abstract：BACKGROUND:Despite the presigmoid transpetrosal approach has been used by different researchers in various ways, the surgical injury rate remains high. Applying a minimally invasive keyhole idea, we devised a presigmoid transpetrosal keyhole approach (PTKA), classified and quantitatively assessed their approach to the ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-04-20 00:00:00
abstract：BACKGROUND:Previous studies have showed that photooxidative stress can lead to down-modulation of nuclear factor-kappa B (NF-kappaB) activity causing apoptosis of cultured photoreceptor cells. This study aimed at investigating whether NF-kappaB was involved in photoreceptor cells apoptosis induced by N-methyl-N-nitroso...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2005-07-05 00:00:00
abstract：BACKGROUND:Anisodamine is widely used in therapy for treating acute glomerulonephritis and diabetic nephropathy because it can improve renal microcirculation. We performed a study to evaluate the preventive effects of anisodamine against contrast-induced nephropathy (CIN) in type 2 diabetics with renal insufficiency un...
journal_title：Chinese medical journal
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2012-10-01 00:00:00
abstract：BACKGROUND:Using tissue Doppler imaging and conventional echocardiographic technique, we examined the cardiac function and synchronicity in individuals with isolated right bundle branch block (RBBB) or left bundle branch block (LBBB) and assessed the relationship between QRS duration and synchronicity. METHODS:Subject...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-05-20 00:00:00
abstract：:In 123 cases of transient ischemic attacks, 72 were followed up for 22 years. The percentage of complete stroke was 46.5% and the fatality rate 47.9%. Causes of death, survival rate by life table, limitation of indication of neurovascular surgical operation and rehabilitation of survived patients were studied. The cau...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1990-09-01 00:00:00
abstract：:Psoralen (PSO) was found cytotoxic against in vitro cultured human mucoepidermoid carcinoma cells of MEC-1 cell line. Its IC50 value was 8.6 micrograms/ml, and relative antitumor activity (RAA) 15. PSO suppressed DNA synthesis, damaged microvilli and cell membrane, and induced degeneration of mitochondria. It inhibite...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
abstract：OBJECTIVE:To characterize the mRNA expression of CXC chemokine IL-8, CC chemokine monocyte chemothractant protein-1 (MCP-1) and regulated on activation, normal T cell expressed and secreted (RANTES), and a newly defined DC chemokine DC-CK1 as well as the expression of IL-8 receptor, MCP-1 receptor and RANTES receptor i...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：OBJECTIVE:To introduce the imaging characteristics of moyamoya disease (MMD) using high-resolution magnetic resonance imaging (HR-MRI) and to discuss the role of HR-MRI in differentiating MMD from other intracranial artery diseases, especially intracranial atherosclerotic disease (ICAD). DATA SOURCES:This review was b...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.4103/0366-6999.170257
更新日期：2015-12-05 00:00:00
abstract：:An epidemiological study on the incidence and prevalence of Parkinson's disease (PD) was carried out in 29 provinces, cities and autonomous regions in this country in 1986. The survey was conducted in 117 areas, and 566 patients with PD were found among 3,869,162 persons examined, giving a point prevalence for men and...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：: ...
journal_title：Chinese medical journal
pub_type： 信件
doi：10.4103/0366-6999.243565
更新日期：2018-10-20 00:00:00
abstract：OBJECTIVE:To understand whether very low density lipoprotein (VLDL) and oxidatively modified very low density lipoprotein (OX-VLDL) can stimulate vascular smooth muscle cells (SMCs) to express high level of monocyte chemotactic protein (MCP-1) mRNA. METHODS:LDL and VLDL were isolated from normal blood donors by densit...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：BACKGROUND:The goal of surgery in the treatment of intrinsic cerebral tumors is to resect the maximum tumor volume, and to spare the eloquent areas. However, it is difficult to discover the eloquent areas intraoperatively due to individual anatomo-functional variability both for sensori-motor and language functions. Co...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
abstract：BACKGROUND:It has been long suggested that abnormal clinical factors in the body, such as dyslipidemia and diabetes, can affect the presence of atherosclerosis. However, few studies on the effect of factors within the normal range, such as the loss of renal function with age, on the prevalence of atherosclerosis are fe...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-08-01 00:00:00
abstract：BACKGROUND:Green tea has been shown to improve cholesterol metabolism in animal studies, but the molecular mechanisms underlying this function have not been fully understood. Long non-coding RNAs (lncRNAs) have recently emerged as a major class of regulatory molecules involved in a broad range of biological processes a...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.147824
更新日期：2015-01-05 00:00:00
abstract：BACKGROUND:Laparoscopic Heller cardiomyotomy and Dor fundoplication is the surgical procedure of choice for esophageal achalasia. The aim of this study was to investigate the clinical outcome of laparoscopic Heller-Dor procedure in our initial series of 25 patients with achalasia. METHODS:Between October 2003 and Janu...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-03-20 00:00:00
abstract：OBJECTIVE:Parkinson's disease (PD) is featured with motor disorder and nonmotor manifestations including psychological symptoms, autonomic nervous system dysfunction, and paresthesia, which results in great inconvenience to the patients' life. The apolipoprotein (Apo) superfamily, as a group of potentially modifiable b...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.4103/0366-6999.217092
更新日期：2017-11-05 00:00:00
abstract：BACKGROUND:Preconditioning with repeated electroacupuncture (EA) could mimic ischemic preconditioning to induce cerebral ischemic tolerance in rats. The present study was designed to investigate whether mu (micro)-, delta (delta)- or kappa (kappa)-opioid receptors are involved in the neuroprotection induced by repeated...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2007-03-05 00:00:00
abstract：BACKGROUND:Although previous reports had reported the use of temporary internal distraction as an aid to correct severe scoliosis, two-stage surgery strategy (less invasive internal distraction followed by posterior correction and instrumentation) has never been reported in the treatment of patients with severe spinal ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVE:To investigate whether or not estrogen receptor and other sex hormone receptors are present in hemangioma tissues of children. METHODS:Fifty-two specimens of hemangiomas were taken from 52 children. The specimens were doubly stained with hematoxylin-eosin and enzyme-linking affinity immunohistochemistry. The...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：OBJECTIVE:To identify novel tumor suppressor genes at chromosome 3p24-26 in human nasopharyngeal carcinoma (NPC). METHODS:Twenty epithelial-derived expressed sequence tags (EST) were selected from chromosome 3p24-26. RT-PCR and Northern blot were used to detect the expression of the ESTs in NPC cell line, HNE-1, and p...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：BACKGROUND:Esophageal carcinoma is a common kind of malignant tumor and about 90% of which is esophageal squamous cell carcinoma (ESCC), and it has a high incidence in China. In recent years it has been argued that angiogenesis plays a key role in tumor growth and metastasis and it is a complex process influenced by ma...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-05-20 00:00:00
abstract：BACKGROUND:MicroRNAs (miRNAs) are small noncoding regulatory RNAs whose aberrant expression may be observed in many malignancies. However, few data are yet available on human primary medulloblastomas. This work aimed to identify that whether miRNAs would be aberrantly expressed in tumor tissues compared with non-tumoro...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-10-20 00:00:00
abstract：:An outbreak of viral central nervous system infection occurred from May to September, 1991 in Shanghai, China. Totally 376 cases were admitted into our hospital, 54% with encephalitis and 46% with meningoencephalitis. Echovirus type 30 was isolated from CSF and stools of the patients. Only 3 of the patients were under...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：BACKGROUND:Enhanced recovery after surgery (ERAS) protocols are a series of perioperative care to optimize preoperative preparation, prevent postoperative complications, minimize stress, and speed up recovery. This study aimed to assess the impact of ERAS protocols for functional endoscopic sinus surgery (FESS) in pati...
journal_title：Chinese medical journal
pub_type： 杂志文章,随机对照试验
doi：10.1097/CM9.0000000000000060
更新日期：2019-02-05 00:00:00
abstract：BACKGROUND:Cornea epithelial cells play early and crucial roles in the initiation of ocular surface responses to pathogens. Participation of toll-like receptor (TLR) 2 and TLR4, which are major forms of fungi receptors, may be involved in Aspergillus fumigatus induced immune responses. The objective of the present stud...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-03-05 00:00:00
abstract：OBJECTIVE:To determine the role of systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndrome (MODS), and evaluate the progress from SIRS to MODS and the therapeutic strategies for acute necrotizing pancreatitis (ANP). METHODS:Rat ANP models were made by retrograde injection of 3.5% sodium ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：BACKGROUND:Atrial fibrillation (AF) is the most common supraventricular arrhythmia in clinical practice. Chronic atrial fibrillation (CAF) is associated with ionic remodeling. However, little is known about the activity of ATP-sensitive potassium current (IK, ATP) during CAF. So we studied the changes of IK, ATP densit...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2005-07-20 00:00:00
abstract：BACKGROUND:Benign prostate hyperplasia is one of the most common diseases affecting the health of the aging males. Watchful waiting is an acceptable management strategy for benign prostate hyperplasia in which the patient is monitored by the physician but receives no active intervention. The epidemiological data on thi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-12-20 00:00:00
abstract：OBJECTIVE:To define the prevalence, severity and risk factors for lead poisoning in new immigrant children from the mainland of China to Hong Kong, China. METHODS:New immigrant children from the mainland of China under 18 years of age were invited to join the study. Their growth parameters and venous blood lead levels...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：BACKGROUND:Atypical meningioma is one of the rare subtypes of meningioma, which is lacking of optimal consensus on treatment strategies. This study aimed to investigate the radical treatment strategies to improve the long-term outcome of recurrent atypical meningiomas. METHODS:The prognostic factors including the age ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
