Abstract:
:Hemophilia B is a sex-linked recessive bleeding disorder characterized by the presence of either a decreased amount of normal factor IX (FIX) or the presence of a dysfunctional FIX. We have identified a unique mutation in a family with mild hemophilia B. DNA analysis of family members revealed a single base transition in the 8th exon of the FIX gene predicting an amino acid change of Asn 346-->Asp in the catalytic domain. The FIX variant, named FIX Denver, was purified from proband plasma. Kinetic studies of factor X (FX) interactions with normal FIXa or FIXa Denver and phospholipid (PL) showed little difference in kcat but a significant difference when factor VIIIa (FVIIIa) was included in the reaction. Using kinetic assays to infer the Kd of FIXa for FVIIIa, normal FIXa had a Kd of 0.095 nM while that of FIXa Denver was 9.85 nM. The major defect caused by this point mutation is a marked decrease in the affinity of FIXa Denver for factor VIIIa.
journal_name
Thromb Haemostjournal_title
Thrombosis and haemostasisauthors
Lefkowitz JB,Nuss R,Haver T,Jacobson L,Thompson AR,Manco-Johnson Mkeywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
862-70issue
3eissn
0340-6245issn
2567-689Xpii
01090862journal_volume
86pub_type
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