Ultrastructural changes in pancreatic beta cells treated with NGF and dbcAMP.

Abstract:

:Nerve growth factor (NGF) induces morphological and physiological changes in cultured pancreatic beta-cells, including the extension of neurite-like processes. This latter effect is potentiated by dibutyryl cAMP (dbcAMP). beta-cells cultured under these conditions maintain their immunoreactivity to insulin and gamma-amino-butyric acid (GABA). NGF, dbcAMP, and high glucose concentrations also increase the expression of the GABA-synthesizing enzyme glutamic acid decarboxylase-65 in cultured beta-cells. The aim of this work was to study the effect of NGF alone or in combination with dbcAMP on pancreatic beta-cell ultrastructural morphology, after 10 days in culture. We used light microscopy, scanning electron microscopy, and transmission electron microscopy to analyze the modifications in cell surface and neurite-like projections. Morphometric analysis showed that NGF and/or dbcAMP treatment substantially increased the insulin and GABA content in granules and rough endoplasmic reticulum. Given that pancreatic beta-cells express NGF receptors and that NGF is synthesized and secreted by beta-cells, these results further suggest that NGF could have trophic actions on pancreatic hormone synthesis and/or storage.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

González del Pliego M,Aguirre-Benítez E,Sánchez-Soto MC,Larrieta ME,Velázquez-Carranza A,Vidaltamayo R,Meza-Mora M,Zepeda-Rodríguez A,Hernández-Falcón J,Hiriart M

doi

10.1007/s004410100411

keywords:

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

365-78

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

305

pub_type

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