Cytotoxic evaluation of root canal filling materials on primary human oral fibroblast cultures and a permanent hamster cell line.

Abstract:

:To date there has been very little data on the cytotoxic response of different cell lines to root canal sealers. The objective of this study was to determine the cytocompatibility of three different extracts of root canal sealers and to compare the cytotoxic response of these materials on two different primary human oral fibroblasts (derived from gingiva and buccal mucosa) and one permanent hamster cell line (V79 cells). Cytotoxicity was judged using an assay of tetrazolium bromide reduction. The results showed that root canal sealers (AH plus, Canals, and N2) were cytotoxic to primary human oral fibroblast cultures and V79 cells. It was found that N2 was the most toxic root canal sealer among those tested in all cultures. The toxicity decreased in an order of N2 > AH plus approximately = Canals. The sensitivity of toxicity depended on the materials tested and the cell culture system used.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Tai KW,Huang FM,Chang YC

doi

10.1097/00004770-200109000-00004

keywords:

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

571-3

issue

9

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(05)60763-7

journal_volume

27

pub_type

杂志文章
  • Cytotoxicity against various cell lines of lipopolysaccharides purified from Bacteroides, Fusobacterium, and Veillonella isolated from infected root canals.

    abstract::The cytotoxicity against two mesenchymal cell lines, L-929 and WI-38, and two epithelial cell lines, KB and HeLa S-3, of lipopolysaccharide (LPS) purified from strains of Bacteroides gingivalis, Fusobacterium nucleatum, and Veillonella parvula isolated from infected root canals was investigated. The inhibition of cell...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(89)80195-5

    authors: Horiba N,Maekawa Y,Abe Y,Ito M,Matsumoto T,Nakamura H,Ozeki M

    更新日期:1989-11-01 00:00:00

  • Severe Pain after Endodontic Surgery: An Analysis of Incidence and Risk Factors.

    abstract:INTRODUCTION:The aim of this study was to determine the prevalence and characteristics of postoperative pain after endodontic microsurgery and to identify potential predictors for severe pain. METHODS:One hundred seventy-three patients who underwent endodontic microsurgery at a private practice were included in the st...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2020.12.012

    authors: Malagise CJ,Khalighinejad N,Patel YT,Jalali P,He J

    更新日期:2020-12-23 00:00:00

  • Shaping ability of the conventional nickel-titanium and reciprocating nickel-titanium file systems: a comparative study using micro-computed tomography.

    abstract:INTRODUCTION:This study used micro-computed tomographic imaging to compare the shaping ability of Mtwo (VDW, Munich, Germany), a conventional nickel-titanium file system, and Reciproc (VDW), a reciprocating file system morphologically similar to Mtwo. METHODS:Root canal shaping was performed on the mesiobuccal and dis...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2013.12.032

    authors: Hwang YH,Bae KS,Baek SH,Kum KY,Lee W,Shon WJ,Chang SW

    更新日期:2014-08-01 00:00:00

  • Neuropeptides in the dental pulp: distribution, origins, and correlation.

    abstract::It is known that many factors participate in the process of inflammation and pain transmission. Recently, various neuropeptides have been demonstrated in the intrapulpal nerve fibers immunobiohistochemically. These neuropeptides may play some role in the process of inflammation and transmission of pain sensation. In t...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(06)81566-9

    authors: Wakisaka S

    更新日期:1990-02-01 00:00:00

  • An in vitro comparison of new irrigation and agitation techniques to ultrasonic agitation in removing bacteria from a simulated root canal.

    abstract:INTRODUCTION:This in vitro study compared 3 agitation and 2 irrigation devices to ultrasonic agitation at mechanically removing bacteria from a plastic simulated canal, instrumented to 35/.06. METHODS:The plastic blocks were divided into seven groups. The control (C) group with brain-heart infusion (BHI) broth (steril...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2009.04.007

    authors: Townsend C,Maki J

    更新日期:2009-07-01 00:00:00

  • A comparison of amounts of apically extruded debris using handpiece-driven nickel-titanium instrument systems.

    abstract::One hundred single-rooted teeth were instrumented with Lightspeed, Profile .04 Taper Series 29, and NT McXIM instruments according to the recommendations of the manufacturers, and Flex-R files using the balanced force technique. Groups were comparable with respect to average curvature, canal length, and major and mino...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(98)80086-1

    authors: Hinrichs RE,Walker WA 3rd,Schindler WG

    更新日期:1998-02-01 00:00:00

  • Comparison of the efficacy of maleic acid and ethylenediaminetetraacetic acid in smear layer removal from instrumented human root canal: a scanning electron microscopic study.

    abstract:INTRODUCTION:The aim of this study was to assess, by scanning electron microscopic (SEM) analysis, the ability of 17% EDTA and 7% maleic acid in the removal of the smear layer from the human root canal system. METHODS:Eighty single-rooted human anterior teeth were subjected to standardized root canal instrumentation (...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.joen.2009.07.021

    authors: Ballal NV,Kandian S,Mala K,Bhat KS,Acharya S

    更新日期:2009-11-01 00:00:00

  • Similar influence of stabilized alkaline and neutral sodium hypochlorite solutions on the fracture resistance of root canal-treated bovine teeth.

    abstract:INTRODUCTION:Stabilizing sodium hypochlorite (NaOCl) at an alkaline pH is proposed to increase solution stability and tissue dissolution ability; however, a reduction on the flexural strength of dentin discs has been found to be a side effect. This study sought to determine whether a stabilized alkaline NaOCl reduces t...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2014.02.028

    authors: Souza EM,Calixto AM,Lima CN,Pappen FG,De-Deus G

    更新日期:2014-10-01 00:00:00

  • Comparison of seal after obturation techniques using a polydimethylsiloxane-based root canal sealer.

    abstract::A polydimethylsiloxane-based sealer (GuttaFlow) was used for filling single-rooted teeth with four obturation techniques. Prefitted gutta-percha master-cones were employed in groups 1 to 3 with backfilling of the sealer. Apical third of the roots were first filled with GuttaFlow, and sealer-coated master cones were se...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2006.07.009

    authors: Brackett MG,Martin R,Sword J,Oxford C,Rueggeberg FA,Tay FR,Pashley DH

    更新日期:2006-12-01 00:00:00

  • Comparative assessment of ActiV GP/glass ionomer sealer, Resilon/Epiphany, and gutta-percha/AH plus obturation: a bacterial leakage study.

    abstract::The objective of this study was to compare the sealing ability of ActiV GP/glass ionomer (GI) sealer (Brasseler USA, Savannah, GA), Resilon/Epiphany (Pentron Clinical Technologies, Wallingford, CT), and gutta-percha (GP)/AH Plus (Dentsply Maillefer, Tulsa, OK). Seventy-three human single-rooted teeth were randomly div...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2008.03.006

    authors: Fransen JN,He J,Glickman GN,Rios A,Shulman JD,Honeyman A

    更新日期:2008-06-01 00:00:00

  • A simplified model to demonstrate the operation of electronic root canal measuring devices.

    abstract::This article describes how to construct and use a simplified model to demonstrate the operation of electronic root canal measuring devices. The model is effective, more economical than those previously described, and uses readily available materials. ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(06)81292-6

    authors: Donnelly JC

    更新日期:1993-11-01 00:00:00

  • Effects of Various Cryotherapy Applications on Postoperative Pain in Molar Teeth with Symptomatic Apical Periodontitis: A Preliminary Randomized Prospective Clinical Trial.

    abstract:INTRODUCTION:The purpose of the study was to evaluate the effects of intracanal, intraoral, and extraoral cryotherapy on postoperative pain in molar teeth with symptomatic apical periodontitis. METHODS:A total of 100 patients were randomly distributed into 4 groups: control (without cryotherapy application), intracana...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.joen.2017.11.002

    authors: Gundogdu EC,Arslan H

    更新日期:2018-03-01 00:00:00

  • Evaluation of root canal transportation, centering ratio, and remaining dentin thickness associated with ProTaper Next instruments with and without glide path.

    abstract:INTRODUCTION:The purpose of this study was to evaluate and compare the volume of removed dentin, transportation, and centering ability of ProTaper Next (PTN) system with and without glide path preparation by using cone-beam computed tomography (CBCT) imaging. METHODS:Sixty mesiobuccal canals of mandibular first molars...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2014.09.001

    authors: Elnaghy AM,Elsaka SE

    更新日期:2014-12-01 00:00:00

  • Incidence of endodontic treatment: a 48-month prospective study.

    abstract::The purpose of this study was to determine the incidence of endodontic services provided to participants in a longitudinal cohort study. The "Florida Dental Care Study" was a prospective cohort study using a representative baseline sample of 873 dentate adults. An in-person interview and clinical dental exam were cond...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200312000-00005

    authors: Boykin MJ,Gilbert GH,Tilashalski KR,Shelton BJ

    更新日期:2003-12-01 00:00:00

  • The quality of ultrasonic root-end preparation: a quantitative study.

    abstract::Ultrasonic root-end preparation techniques have recently been introduced and revolutionized the field of endodontic surgery. The purpose of this study was to compare quantitatively the quality of root-end preparation techniques prepared by a specially designed ultrasonic retrotip with those prepared in a traditional m...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(98)80152-0

    authors: Lin CP,Chou HG,Kuo JC,Lan WH

    更新日期:1998-10-01 00:00:00

  • Patterns of vertical root fracture: factors affecting stress distribution in the root canal.

    abstract::Previous studies have indicated that vertical root fracture tends to occur in a buccolingual direction, where dentin thickness is greatest. Factors potentially influencing the location and direction of root fracture include root canal shape, external root morphology, and dentin thickness. In this finite-element study,...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200308000-00008

    authors: Lertchirakarn V,Palamara JE,Messer HH

    更新日期:2003-08-01 00:00:00

  • Calcium ions released from mineral trioxide aggregate convert the differentiation pathway of C2C12 cells into osteoblast lineage.

    abstract:INTRODUCTION:The purpose of this study was to examine the effect of mineral trioxide aggregate (MTA) on pluripotent-mesenchymal cell differentiation. METHODS:The pluripotent-mesenchymal cell line C2C12 was cultured in a 5% serum medium to induce cell differentiation with or without MTA. The differentiation to myoblast...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2012.10.006

    authors: Matsumoto S,Hayashi M,Suzuki Y,Suzuki N,Maeno M,Ogiso B

    更新日期:2013-01-01 00:00:00

  • Sealing properties of different obturation systems applied over apically fractured rotary nickel-titanium files.

    abstract::This study investigated the sealing properties of cold laterally compacted gutta percha and Thermafil applied over different apically fractured rotary nickel-titanium files. Extracted human premolars were prepared by using ProTaper (Dentsply-Maillefer, Ballaigues, Switzerland) or ProFile (Dentsply-Maillefer) systems (...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2007.10.023

    authors: Altundasar E,Sahin C,Ozcelik B,Cehreli ZC

    更新日期:2008-02-01 00:00:00

  • An evaluation of canal morphology at different levels of root resection in mandibular incisors.

    abstract::One hundred randomly selected mandibular incisors were examined to assess the prevalence and location of two canals and to describe the canal anatomy that may be encountered during apical surgery. Sections of the root were cut at 1, 2, and 3 mm from the apex, simulating a 20-degree beveled surgical resection. The sect...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(98)80120-9

    authors: Mauger MJ,Schindler WG,Walker WA 3rd

    更新日期:1998-09-01 00:00:00

  • Isolation of methicillin-resistant staphylococci in the dental operatory.

    abstract::The state of contamination by methicillin-resistant Staphylococci, including methicillin-resistant Staphylococcus aureus (MRSA) at the conservative dentistry section of the School of Dentistry, Aichi-Gakuin University, was investigated. Methicillin-resistant coagulase-negative Staphylococci (MRCNS) were detected in 8 ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(06)80552-2

    authors: Horiba N,Yoshida T,Suzuki K,Maekawa Y,Ito M,Matsumoto T,Nakamura H

    更新日期:1995-01-01 00:00:00

  • A radiographic, histological, and histometric study of endodontic materials.

    abstract::Silicone tubes filled with CRCS, a calcium hydroxide-based cement, were implanted at two different localizations in the right tibias of white male Wistar rats. A similar number of solid silicone rods were implanted in the left tibias of the same animals and were used as inert controls. Fifteen days after implantation ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(89)80090-1

    authors: Guglielmotti MB,Zmener O,Cabrini RL

    更新日期:1989-01-01 00:00:00

  • Combined Endodontic Therapy and Intentional Replantation for the Treatment of Palatogingival Groove.

    abstract::A palatogingival groove is an anatomic malformation that predisposes the involved tooth to a severe periodontal defect. When the condition is complicated by pulpal necrosis, affected teeth often present a dilemma in terms of diagnosis and treatment planning. In this report, we describe the case of a patient with a max...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2015.10.009

    authors: Garrido I,Abella F,Ordinola-Zapata R,Duran-Sindreu F,Roig M

    更新日期:2016-02-01 00:00:00

  • Expression of Mineralization Markers during Pulp Response to Biodentine and Mineral Trioxide Aggregate.

    abstract:INTRODUCTION:The purpose of this study was to compare the cell viability of dental pulp cells treated with Biodentine (Septodont, Saint-Maur, France) and mineral trioxide aggregate (MTA) and the in vitro and in vivo expression of mineralization markers induced by the 2 materials. METHODS:Human dental pulp cells isolat...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2015.12.018

    authors: Daltoé MO,Paula-Silva FW,Faccioli LH,Gatón-Hernández PM,De Rossi A,Bezerra Silva LA

    更新日期:2016-04-01 00:00:00

  • Relationship of radiologic and histologic signs of inflammation in human root-filled teeth.

    abstract::The aim of this study was to determine the relationship between histologic and radiologic signs of inflammation in human root-filled teeth. In addition, other factors with possible importance for apical inflammation were assessed. Fifty-three block sections of root-filled teeth were gathered from human cadavers. The b...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200402000-00003

    authors: Barthel CR,Zimmer S,Trope M

    更新日期:2004-02-01 00:00:00

  • Conventional and surgical treatment of a two-rooted maxillary central incisor.

    abstract::Conventional and surgical endodontic management were performed on a two-rooted maxillary central incisor with a periapical lesion. Anatomical complexity of the tooth lead to perforation in the distal root during conventional treatment, thus a surgical approach was taken. A 4-month postoperative radiograph shows repair...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200306000-00011

    authors: González-Plata-R R,González-Plata-E W

    更新日期:2003-06-01 00:00:00

  • Susceptibilities of Enterococcus faecalis biofilms to some antimicrobial medications.

    abstract::Enterococcus faecalis has been suggested to be an important etiological agent in endodontic failures. The purpose of this study was to evaluate the effectiveness of chlorhexidine- or antibiotics-based medications in eliminating E. faecalis biofilms. One-day and three-day biofilms of E. faecalis were induced on cellulo...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200110000-00004

    authors: Lima KC,Fava LR,Siqueira JF Jr

    更新日期:2001-10-01 00:00:00

  • Cracked Teeth and Poor Oral Masticatory Habits: A Matched Case-control Study in China.

    abstract:INTRODUCTION:Previous studies have suggested that masticatory forces are associated with cracked teeth, but it is not clear which specific poor oral masticatory habits may be more likely to cause cracks. The aim of this study was to quantitatively assess risk factors for cracked teeth among poor oral masticatory habits...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2017.01.007

    authors: Qiao F,Chen M,Hu X,Niu K,Zhang X,Li Y,Wu Z,Shen Z,Wu L

    更新日期:2017-06-01 00:00:00

  • A method to determine the location and shape of an intracoronal bleach barrier.

    abstract::The external cervical root resorption associated with intracoronal bleaching of pulpless teeth can be a devastating lesion. It often cannot be repaired. To prevent this problem, increasing attention has been focused on placing a barrier between the pulp chamber and the endodontic filling material. The objective of thi...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(06)80822-8

    authors: Steiner DR,West JD

    更新日期:1994-06-01 00:00:00

  • Disinfecting and Shaping Type I C-shaped Root Canals: A Correlative Micro-computed Tomographic and Molecular Microbiology Study.

    abstract:INTRODUCTION:This ex vivo study compared the disinfection and shaping abilities of 2 preparation protocols in C-shaped canals. METHODS:Mandibular second molars with type I C-shaped canals were pair matched based on micro-computed tomographic (micro-CT) analysis and distributed into 2 groups. The canals were contaminat...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2020.11.007

    authors: Gazzaneo I,Amoroso-Silva P,Pacheco-Yanes J,Alves FRF,Marceliano-Alves M,Olivares P,Meto A,Mdala I,Siqueira JF Jr,Rôças IN

    更新日期:2020-11-18 00:00:00

  • Histological and densitometric analysis of the effects of exogeneous heparin on rat pulp tissues.

    abstract::In this study, the effects of heparin on dentinal resorption was investigated in rats. Animals were injected with 2 x 2 IU/g Na-Heparin subcutaneously for 33 days. Histopathological examination of the anterior teeth revealed capillary proliferation, congestion, and fibrosis in the pulp in addition to resorptive lacuna...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(99)80347-1

    authors: Kuraner T,Ozçelik B,Serper A,Mocan G,Mümin E,Eryilmaz M

    更新日期:1999-10-01 00:00:00