Abstract：
AIM:To determine whether a combination of cell targeting and sequence recognition of nucleic acids can provide specificity for the inhibition of viral gene expression. METHODS:Antisense oligonucleotides complexed to a protein-based DNA carrier system were used to target hepatocytes for the inhibition of human hepatitis viral gene expression. The DNA carrier system contained an asialoglycoprotein as a cell-targeting component, which could direct the uptake of complexed DNA specifically to asialoglycoprotein receptors present selectively on the surface of mammalian hepatocytes. RESULTS:HBV and HCV viral gene expression were substantially and specifically inhibited by use of antisense oligonucleotides complexed to a protein-based DNA carrier system. CONCLUSION:Targeted delivery of nucleic acids by use of receptor-mediated endocytosis can result in inhibition of viral gene expression without host toxicity.
journal_name
Croat Med Jjournal_title
Croatian medical journalauthors
Wu GY,Walton CM,Wu CHkeywords:
subject
Has Abstractpub_date
2001-08-01 00:00:00pages
463-6issue
4eissn
0353-9504issn
1332-8166journal_volume
42pub_type
杂志文章abstract：AIM:To determine the prevalence of bronchial asthma among Saudi Arabian schoolboys living in Al-Khobar city. METHODS:Parents of 1,482 randomly selected boys in preparatory and elementary schools included in this cross-sectional study filled out a questionnaire on asthma signs in their sons. Number of Questionnaire-Dia...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：AIM:To analyze clinical and functional effects of intra-articular injection of autologous micro-fragmented lipoaspirate (MLA) in patients with late stage knee osteoarthritis (KOA). Secondary aims included classifying cell types contributing to the treatment effect, performing detailed MRI-based classification of KOA, a...
journal_title：Croatian medical journal
pub_type： 临床试验,杂志文章
doi：
更新日期：2019-06-13 00:00:00
abstract：AIM:To evaluate psychosomatic complaints and depressive symptoms among civilians, refugees, and soldiers in the war and post-war period in Croatia. METHOD:The design of the study was longitudinal, including four repeated assessments during the war and post-war period (1993, 1995, 2000, and 2004). The baseline assessme...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-04-01 00:00:00
abstract：AIM:To evaluate the efficacy and toxicity of preoperative chemoradiotherapy with capecitabine in locally advanced rectal cancer. METHODS:Between June 2004 and January 2005, 57 patients with operable, clinical stage II-III adenocarcinoma of the rectum entered the prospective phase II study. Radiation dose was 45 Gy (25...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2006-10-01 00:00:00
abstract：:Some aspects of the "impact factor", a quantitative measure of journals' influence on journals in scientific fields, were discussed in the preceding issue of the Croatian Medical Journal by Dr Eugene Garfield, one of its devisers. This factor can be of interest to authors, journal editors, and policy makers, but they ...
journal_title：Croatian medical journal
pub_type： 社论
doi：
更新日期：2001-02-01 00:00:00
abstract：AIM:To determine types and frequency of CAPN3 mutations in 29 unrelated Croatian families, analyzed during 6-year prospective and ongoing genetic and epidemiological study of muscular dystrophies in Croatia. METHODS:Mutation analysis included allele-specific polymerase chain reaction (PCR) or combination of PCR and re...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-08-01 00:00:00
abstract：:The article considers available options to educate science editors in the fast-transforming digital environment. There is no single course or resource that can cover their constantly changing and diversifying educational needs. The involvement in research, writing, and reviewing is important for gaining editing skills...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2014.55.672
更新日期：2014-12-01 00:00:00
abstract：AIM:To compare the health-related quality of life of unemployed and employed women with disabilities and establish factors affecting their life satisfaction. METHODS:The study included 318 women with disabilities, 160 of whom were employed and 158 unemployed, paired according to age and region of residence. The health...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2011.52.550
更新日期：2011-08-15 00:00:00
abstract：AIM:To examine the relationship between war experiences and war-related distress in Bosnia and Herzegovina. METHODS:The survey was performed in the late 2003 on a representative sample of 3313 respondents. The face-to-face interviews included 15 items on war-related distress and 24 items on war experiences. From these...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2008.1.75
更新日期：2008-02-01 00:00:00
abstract：AIM:To evaluate successes in the clinical management of severe diarrheal diseases in a busy referral hospital in Ghana, four years after the introduction of the World Health Organization's protocol for the clinical management of diarrhea and the establishment of an oral rehydration therapy Corner. METHOD:Data on the c...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：AIM:To investigate the ability of two standard quality of life (QOL) questionnaires - The Short Form (36-item) Health Survey (SF-36) and The European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire-Core 30 (EORTC QLQ C30) to evaluate QOL in patients with chronic graft-vs-host disease (cG...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2016.57.6
更新日期：2016-02-01 00:00:00
abstract：AIM:To report on our experience with a voice-directed robotic arm for scope management in different procedures for "solo-surgery" and in complex laparoscopic operations. METHODS:A chip card with orders for the robotic arm is individually manufactured for every user. A surgeon gives order through a microphone and the o...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:Kidney transplantation is the most efficient method of renal replacement therapy. When this method is performed, native urinary bladder is the preferred urinary reservoir. However, in some patients with an anatomically and functionally abnormal lower urinary tract, the urinary bladder cannot be used for transplantatio...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2019-12-31 00:00:00
abstract：AIM:To determine the prevalence of human immunodeficiency virus (HIV) infection in high-risk groups, ie, injecting drug users, patients with sexually transmitted infections, and men who have sex with men, and compare it with the prevalence determined in low-risk group, ie, pregnant women. METHODS:Residual sera from sp...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2003-10-01 00:00:00
abstract：AIM:To examine the usefulness of molecular analysis of IgH gene rearrangement in assessment of clonality in bone marrow biopsies with lymphoid aggregates (LA) and/or nodular lymphoid hyperplasia (NLH) in patients with different subtypes of malignant lymphomas. METHOD:Five hundred and twenty nine samples of bone marrow...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
abstract：: ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2019-02-28 00:00:00
abstract：:Corporate medical practice, a market economy, and a consumer culture are transforming health care. The service relationships of doctors with patients are now commodities. The doctor, directed by disease management protocols (to improve outcomes, reduce costs, and standardize care), is, in effect, providing programmed ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：AIM:To analyze the haplotype of the Ezhava population of Kerala, south India, using 8 short tandem repeat (STR) loci on the Y chromosome and trace the paternal genetic lineage of the population. METHODS:Whole blood samples (n=104) were collected from unrelated healthy men of the Ezhava population over a period of one ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2011.52.344
更新日期：2011-06-01 00:00:00
abstract：AIM:To identify and characterize the subunit structure of interleukin-13 receptor (IL-13R) in human medulloblastoma cell lines and target them with a chimeric fusion protein consisting of interleukin-13 and Pseudomonas exotoxin (termed as IL-13 cytotoxin). METHODS:Five human medulloblastoma cell lines were examined fo...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2003-08-01 00:00:00
abstract：AIM:To assess the diagnostic value of neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) in lung cancer (LC). We compared the ratios between healthy participants and all LC patients, as well patients with different pathohistological LC subtypes. METHODS:We retrieved the data on neutrophil, lym...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2016.57.287
更新日期：2016-06-30 00:00:00
abstract：AIM:To compare morbidity and mortality of patients with severe intra-abdominal infections after two types of surgical treatment, on-demand ("wait and see") relaparotomy and modified planned relaparotomy. METHODS:We prospectively analyzed the outcomes of 65 patients with severe peritonitis surgically treated in two Cro...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：AIM:To examine relationships among combat exposure, posttraumatic stress disorder (PTSD) symptoms, depression, suicidality, nicotine dependence, and religiosity in Croatian veterans. METHODS:This cross-sectional study used Combat Exposure Scale (CES) to quantify the stressor severity, PTSD Checklist 5 (PCL) to quantif...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2018-08-31 00:00:00
abstract：AIM:To determine sociodemographic features that describe mothers who are likely to wean their babies earlier then is recommended and whether the rates of breast-fed children in Croatia had improved since 1996. METHODS:A sample of 500 mothers was selected from the population of women who had delivered 2 to 12 months be...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2003-10-01 00:00:00
abstract：AIM:To assess the usefulness of a DNA-based method for identifying mushroom species for application in forensic laboratory practice. METHODS:Two hundred twenty-one samples of clinical forensic material (dried mushrooms, food remains, stomach contents, feces, etc) were analyzed. ITS2 region of nuclear ribosomal DNA (nr...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2015.56.32
更新日期：2015-02-01 00:00:00
abstract：:Academic medicine integrates three of the most honorable human activities: treating the ill, teaching, and research. The quality that all three share is persistent quest for truth. However, there is reluctance of academic medicine today to openly defend scientific truth by challenging the arguments and the very existe...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2004-12-01 00:00:00
abstract：AIM:To investigate whether socioeconomic inequalities at a micro-scale, through their effect on major health risk factors and other health indicators, contribute to health status in an isolated island population with demonstrated reduced genetic and environmental variability. METHODS:This cross-sectional study was per...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2007-10-01 00:00:00
abstract：AIM:To evaluate risk factors related to total mortality in an unselected population of patients implanted with a cardioverter defibrillator. METHODS:Survival analysis was performed retrospectively investigating the records of 77 consecutive patients implanted with defibrillators (median 67 years, range 38-83 years; 63...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2007-02-01 00:00:00
abstract：AIM:To detect polymorphisms of 23 Y-chromosomal short tandem repeat (STR) loci, including 6 new loci, in a reference database of male population of Bosnia and Herzegovina, as well as to assess the importance of increasing the number of Y-STR loci utilized in forensic DNA analysis. METHODS:The reference sample consiste...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2013.54.286
更新日期：2013-06-01 00:00:00
abstract：:This article brings an overview of the influence of molecular medicine on pathophysiology, medical practice, and medical education. Various aspects of the growing impact of molecular medicine on clinical practice are discussed: diagnostic and predictive testing, gene and targeted therapy, and pharmacogenomics. Insuffi...
journal_title：Croatian medical journal
pub_type： 社论
doi：
更新日期：2003-08-01 00:00:00
abstract：AIM:To compare primary and secondary wound reconstruction as a treatment method for Gustilo type III open tibial fractures. METHODS:Thirty-five consecutive patients with a Gustilo type III open tibial shaft fracture were treated and followed up for 3 years. The patients were divided into two groups depending on the tr...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
