Dominant loss of responsiveness to sweet and bitter compounds caused by a single mutation in alpha -gustducin.

abstract：:Transposition is a multistep process in which a transposable element DNA sequence moves from its original genetic location to a new site. Early steps in this process include the formation of a transposition complex in which the end sequences of the transposable element are brought together in a structurally precise fa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goryshin IYu,Kil YV,Reznikoff WS

更新日期：1994-11-08 00:00:00

abstract：:Immune responses can interfere with the effective use of therapeutic proteins to treat genetic deficiencies and have been challenging to manage. To address this problem, we adapted and studied methods of immune tolerance used in canine organ transplantation research to soluble protein therapeutics. A tolerization regi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kakkis E,Lester T,Yang R,Tanaka C,Anand V,Lemontt J,Peinovich M,Passage M

更新日期：2004-01-20 00:00:00

abstract：:Recent advances in high-field MRI have dramatically improved the visualization of human brain anatomy in vivo. Most notably, in cortical gray matter, strong contrast variations have been observed that appear to reflect the local laminar architecture. This contrast has been attributed to subtle variations in the magnet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fukunaga M,Li TQ,van Gelderen P,de Zwart JA,Shmueli K,Yao B,Lee J,Maric D,Aronova MA,Zhang G,Leapman RD,Schenck JF,Merkle H,Duyn JH

更新日期：2010-02-23 00:00:00

abstract：:The AF-6 protein is a multidomain protein that contains two potential Ras-binding domains within its N terminus. Because of this feature, AF-6 has been isolated in both two-hybrid and biochemical approaches and is postulated to be a potential Ras-effector protein. Herein, we show that it is specifically the first Ras-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boettner B,Govek EE,Cross J,Van Aelst L

更新日期：2000-08-01 00:00:00

abstract：:Column chromatography on (dT)(12-18)-cellulose was used to detect and isolate a novel DNA polymerase activity from human breast tumor cell line HBT-3. The new enzyme is similar to those of mammalian RNA tumor viruses in its molecular size, preference for poly(A).(dT)(12-18) as compared to either poly(dA).(dT)(12-18) o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerwin BI,Bassin RH

更新日期：1973-08-01 00:00:00

abstract：:Friction is caused by dissipative lateral forces that act between macroscopic objects. An improved understanding of friction is therefore expected from measurements of dissipative lateral forces acting between individual atoms. Here we establish atomic resolution of both conservative and dissipative forces by lateral ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giessibl FJ,Herz M,Mannhart J

更新日期：2002-09-17 00:00:00

abstract：:Ocean acidification describes the progressive, global reduction in seawater pH that is currently underway because of the accelerating oceanic uptake of atmospheric CO(2). Acidification is expected to reduce coral reef calcification and increase reef dissolution. Inorganic cementation in reefs describes the precipitati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manzello DP,Kleypas JA,Budd DA,Eakin CM,Glynn PW,Langdon C

更新日期：2008-07-29 00:00:00

abstract：:Functional transcription elongation complexes can be formed by adding RNA polymerase in trans to a preformed nucleic acid construct. This construct consists of a double-stranded DNA fragment that contains a noncomplementary (permanent DNA bubble) region into which an RNA primer oligonucleotide has been hybridized. By ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daube SS,Hart CR,von Hippel PH

更新日期：1994-09-27 00:00:00

abstract：:Somatic fungal hyphae are generally assumed to elongate at steady linear rates when grown under constant environmental conditions with ample nutrients. However, patterns of pulsed hyphal elongation were detected during apparent steady growth of hyphal tips in fungi from several major taxonomic groups (Oomycetes, Pythi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： López-Franco R,Bartnicki-Garcia S,Bracker CE

更新日期：1994-12-06 00:00:00

abstract：:Proceedings of the National Academy of Sciences Colloquium on the roles of homologous recombination in DNA replication are summarized. Current findings in experimental systems ranging from bacteriophages to mammalian cell lines substantiate the idea that homologous recombination is a system supporting DNA replication ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kuzminov A

更新日期：2001-07-17 00:00:00

abstract：:Productive T cell recognition of antigen-presenting cells (APCs) is normally accompanied by the formation of a cell-cell contact called the "immunological synapse." Our understanding of the steps leading up to this formation has been limited by the absence of tools for analyzing 3D surfaces and surface distributions a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moss WC,Irvine DJ,Davis MM,Krummel MF

更新日期：2002-11-12 00:00:00

abstract：:A cyclic AMP-binding protein (CAP protein), cyclic AMP, and RNA polymerase holoenzyme are shown to initiate lac transcription at the lac promoter. Lac repressor appears to control transcription by preventing RNA polymerase and/or CAP protein from binding to the lac promoter. Results support the idea that the lac promo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eron L,Block R

更新日期：1971-08-01 00:00:00

abstract：:The receptor for acetylcholine was partially purified by affinity chromatography of an extract in Triton X-100 of membrane fragments from electric tissue. The receptor was assayed, after its reduction with dithiothreitol, by reaction with the affinity-alkylating agent, [methyl-(3)H]4-(N-maleimido)-benzyltrimethylammon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karlin A,Cowburn D

更新日期：1973-12-01 00:00:00

abstract：:p19ARF has been implicated as a key regulator of p53 stability and activation. While numerous stresses activate the p53 growth arrest pathway, those requiring p19ARF remain to be elucidated. We used p19ARF knockout mouse embryo fibroblasts to show that DNA damage and microtubule disruption require p19ARF to induce p53...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khan SH,Moritsugu J,Wahl GM

更新日期：2000-03-28 00:00:00

abstract：:Absorbance changes of C-550 and cytochrome b(559), and fluorescence-yield changes were measured during irradiation of chloroplasts at -196 degrees . The photo-reduction of C-550 proceeded more rapidly than the photo-oxidation of cytochrome b(559), and the fluorescence-yield change had similar kinetics to the cytochrom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler WL

更新日期：1972-11-01 00:00:00

abstract：:Knowing how motile bacteria move near and along a solid surface is crucial to understanding such diverse phenomena as the migration of infectious bacteria along a catheter, biofilm growth, and the movement of bacteria through the pore spaces of saturated soil, a critical step in the in situ bioremediation of contamina...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frymier PD,Ford RM,Berg HC,Cummings PT

更新日期：1995-06-20 00:00:00

abstract：:The density of cells and neurons in the neocortex of many mammals varies across cortical areas and regions. This variability is, perhaps, most pronounced in primates. Nonuniformity in the composition of cortex suggests regions of the cortex have different specializations. Specifically, regions with densely packed neur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collins CE,Turner EC,Sawyer EK,Reed JL,Young NA,Flaherty DK,Kaas JH

更新日期：2016-01-19 00:00:00

abstract：:We have used fast time-resolved infrared spectroscopy to characterize a series of organometallic methane and ethane complexes in solution at room temperature: W(CO)5(CH4) and M(eta5-C5R5)(CO)2(L) [where M = Mn or Re, R = H or CH3 (Re only); and L = CH4 or C2H6]. In all cases, the methane complexes are found to be shor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cowan AJ,Portius P,Kawanami HK,Jina OS,Grills DC,Sun XZ,McMaster J,George MW

更新日期：2007-04-24 00:00:00

abstract：:Selective therapeutics for nuclear receptors would revolutionize treatment for endocrine disease. Specific control of nuclear receptor activity is challenging because the internal cavities that bind hormones can be virtually identical. Only one highly selective hormone analog is known for the thyroid receptor, GC-24, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Borngraeber S,Budny MJ,Chiellini G,Cunha-Lima ST,Togashi M,Webb P,Baxter JD,Scanlan TS,Fletterick RJ

更新日期：2003-12-23 00:00:00

abstract：:Systemic inflammation occurring around the course of tumor progression and treatment are often correlated with adverse oncological outcomes. As such, it is suspected that neutrophils, the first line of defense against infection, may play important roles in linking inflammation and metastatic seeding. To decipher the d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen MB,Hajal C,Benjamin DC,Yu C,Azizgolshani H,Hynes RO,Kamm RD

更新日期：2018-07-03 00:00:00

abstract：:A critique is presented of the Lumsden-Wilson theory [Lumsden, C. J. & Wilson, E. O. (1980) Proc. Natl. Acad. Sci. USA 77, 4382-4386] of the transmission of cultural traits. An analysis of the underlying assumptions and the mathematical nature of the theory clarifies its essentially reductionist and determinist qualit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alper JS,Lange RV

更新日期：1981-06-01 00:00:00

abstract：:Steroidogenic factor-1 (SF-1) and liver receptor homologue-1 (LRH-1) belong to the fushi tarazu factor 1 subfamily of nuclear receptors. SF-1 is an essential factor for sex determination during development and regulates adrenal and gonadal steroidogenesis in the adult, whereas LRH-1 is a critical factor for developmen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang W,Zhang C,Marimuthu A,Krupka HI,Tabrizizad M,Shelloe R,Mehra U,Eng K,Nguyen H,Settachatgul C,Powell B,Milburn MV,West BL

更新日期：2005-05-24 00:00:00

abstract：:The failure of the population of microjunctions forming the frictional interface between two solids is central to fields ranging from biomechanics to seismology. This failure is mediated by the propagation along the interface of various types of rupture fronts, covering a wide range of velocities. Among them are the s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Trømborg JK,Sveinsson HA,Scheibert J,Thøgersen K,Amundsen DS,Malthe-Sørenssen A

更新日期：2014-06-17 00:00:00

abstract：:We have used oligonucleotide-directed mutagenesis to make a specific change in the beta-lactamase (EC 3.5.2.6) (ampicillin resistance) gene of the plasmid pBR322. Evidence suggests that the active site for this enzyme may include a serine-threonine dyad (residues 70 and 71). By priming in vitro DNA synthesis with a ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dalbadie-McFarland G,Cohen LW,Riggs AD,Morin C,Itakura K,Richards JH

更新日期：1982-11-01 00:00:00

abstract：:A long-term electrophoretic survey of genetic variants of serum albumin has identified an alloalbumin in 589 unrelated individuals in Italy. The alloalbumins were classified electrophoretically into 17 types. The number of unrelated carriers for each type varied from 1 for several variants reported here to 103 for alb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galliano M,Minchiotti L,Porta F,Rossi A,Ferri G,Madison J,Watkins S,Putnam FW

更新日期：1990-11-01 00:00:00

abstract：:Forkhead box P3 (FOXP3)-positive Treg cells are crucial for maintaining immune homeostasis. FOXP3 cooperates with its binding partners to elicit Treg cells' signature and function, but the molecular mechanisms underlying the modulation of the FOXP3 complex remain unclear. Here we report that Deleted in breast cancer 1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao Y,Tang J,Chen W,Li Q,Nie J,Lin F,Wu Q,Chen Z,Gao Z,Fan H,Tsun A,Shen J,Chen G,Liu Z,Lou Z,Olsen NJ,Zheng SG,Li B

更新日期：2015-06-23 00:00:00

abstract：:During natural fertilization, mammalian spermatozoa must pass through the zona pellucida before reaching the plasma membrane of the oocyte. It is assumed that this step involves partial lysis of the zona by sperm acrosomal enzymes, but there has been no unequivocal evidence to support this view. Here we present eviden...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirose M,Honda A,Fulka H,Tamura-Nakano M,Matoba S,Tomishima T,Mochida K,Hasegawa A,Nagashima K,Inoue K,Ohtsuka M,Baba T,Yanagimachi R,Ogura A

更新日期：2020-02-04 00:00:00

abstract：:The in situ stimulation of Fe(III) oxide reduction by Geobacter bacteria leads to the concomitant precipitation of hexavalent uranium [U(VI)] from groundwater. Despite its promise for the bioremediation of uranium contaminants, the biological mechanism behind this reaction remains elusive. Because Fe(III) oxide reduct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cologgi DL,Lampa-Pastirk S,Speers AM,Kelly SD,Reguera G

更新日期：2011-09-13 00:00:00

abstract：:The proteolytic cleavage of sterol regulatory element-binding proteins (SREBPs) is regulated by SREBP cleavage-activating protein (SCAP), which forms complexes with SREBPs in membranes of the endoplasmic reticulum (ER). In sterol-depleted cells, SCAP facilitates cleavage of SREBPs by Site-1 protease, thereby initiatin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nohturfft A,DeBose-Boyd RA,Scheek S,Goldstein JL,Brown MS

更新日期：1999-09-28 00:00:00

abstract：:We have isolated a 2.7-kilobase rat liver cDNA clone that contains the entire 544-amino acid coding sequence for matrin F/G. This protein has previously been localized to the internal, fibrogranular areas of the nuclear matrix and shown to bind to DNA on nitrocellulose blots. The predicted amino acid sequence from the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hakes DJ,Berezney R

更新日期：1991-07-15 00:00:00