Abstract:
:Treatment of MC3T3E-1 osteoblast cultures with combined interferon- gamma(IFN- gamma), lipopolysaccharide (LPS) and tumor necrosis factor- alpha(TNF- alpha) induces expressions of inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6), resulting in sustained releases of large amounts of nitric oxide and IL-6. However IFN- gamma, LPS and TNF- alpha individually induces non-detectable or small amounts of NO and IL-6 in MC3T3E-1 osteoblasts. The role of mitogen-activated protein kinase (MAPK) activation in the early intracellular signal transduction involved in iNOS and IL-6 transcription in the combined agents-stimulated osteoblasts has been investigated. The p38 MAPK pathway is specifically involved in the combined agents-induced NO and IL-6 release, since NO and IL-6 release in the presence of a specific inhibitor of p38 MAPK, 4-(4-fluorophenyl)-2-(4-metylsulfinylphenyl)-5-(4-pyridyl)imidazole (SB203580), are significantly diminished. In contrast, PD98059, a specific inhibitor of MEK1, had no effect on NO and IL-6 release. Northern blot analysis showed that the p38 MAPK pathway controlled iNOS and IL-6 transcription levels. These data suggest that p38 MAPK plays an important role in the secretion of NO and IL-6 in LPS/IFN- gamma or TNF- alpha /IFN- gamma -treated MC3T3E-1 osteoblasts.
journal_name
Pharmacol Resjournal_title
Pharmacological researchauthors
Chae HJ,Kim SC,Chae SW,An NH,Kim HH,Lee ZH,Kim HRdoi
10.1006/phrs.2000.0778keywords:
subject
Has Abstractpub_date
2001-03-01 00:00:00pages
275-83issue
3eissn
1043-6618issn
1096-1186pii
S1043661800907783journal_volume
43pub_type
杂志文章abstract::Breast cancer is a leading cause of morbidity and mortality in women. Both Epidemiological and experimental studies indicate a positive correlation between alcohol consumption and the risk of breast cancer. While alcohol exposure may promote the carcinogenesis or onset of breast cancer, it may as well enhance the prog...
journal_title:Pharmacological research
pub_type: 杂志文章,评审
doi:10.1016/j.phrs.2016.12.005
更新日期:2017-01-01 00:00:00
abstract::Advanced glycation end products (AGEs) and their receptor (RAGE) axis contributes to diabetic nephropathy. An oral hypoglycemic agent, metformin may have a potential effect on the inhibition of glycation reactions. Further, since a pathophysiological crosstalk between renin-angiotensin system (RAS) and AGEs-RAGE axis ...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2011.11.001
更新日期:2012-03-01 00:00:00
abstract::We previously demonstrated that the typical mitochondrial uncoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP) inhibited artery constriction, but CCCP was used only as a pharmacological tool. Niclosamide is an anthelmintic drug approved by FDA. Niclosamide ethanolamine (NEN) is a salt form of niclosamide and has ...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2016.11.008
更新日期:2017-01-01 00:00:00
abstract::In the past two decades, significant advances have been made in the etiology of lipid disorders. Concomitantly, the discovery of liporegulatory functions of certain short-chain fatty acids has generated interest in their clinical applications. In particular, butyric acid (BA) and its derivative, 4-phenylbutyric acid (...
journal_title:Pharmacological research
pub_type: 杂志文章,评审
doi:10.1016/j.phrs.2019.04.002
更新日期:2019-06-01 00:00:00
abstract::Prader-Willi syndrome (PWS), the leading genetic cause of obesity, is characterized by a striking hyperphagic behavior that can lead to obesity, type-2 diabetes, cardiovascular disease and death. The molecular mechanism underlying impaired satiety in PWS is unknown. Oleoylethanolamide (OEA) is a lipid mediator involve...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2016.12.024
更新日期:2017-03-01 00:00:00
abstract::The mechanism for the pathological increase in cell death in various disease states e.g. HIV immunodefficiency or even ageing or Alzheimer's disease, occurs by complex and as yet undefined mechanism(s) related to immunological, virological or biochemical disturbances (i.e. energy depletion, oxidative stress, increased...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/s1043-6618(05)80044-1
更新日期:1995-12-01 00:00:00
abstract::We tested the efficacy of novel cyclooxygenase 2 (COX-2) inhibitors in counteracting glia-driven neuroinflammation induced by the amyloidogenic prion protein fragment PrP90-231 or lipopolysaccharide (LPS). In search for molecules with higher efficacy than celecoxib, we focused our study on its 2,3-diaryl-1,3-thiazolid...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2016.09.010
更新日期:2016-11-01 00:00:00
abstract::Oxidative stress is an important contributor to the risk of chronic diseases. Dietary guidelines recommend increased consumption of fruits and vegetables to combat the incidence of human diseases such as cancer, cardiovascular disease, osteoporosis and diabetes. Fruits and vegetables are good sources of antioxidant ph...
journal_title:Pharmacological research
pub_type: 杂志文章,评审
doi:10.1016/j.phrs.2007.01.012
更新日期:2007-03-01 00:00:00
abstract::Hypercalcemia and hypercalciuria observed both in humans and in animals who were on long-term theophylline therapy, prompted us to investigate whether oral theophylline treatment at optimal doses causes any adverse side effects on bone metabolism in mild asthmatics. Therefore, serum osteocalcin (BGP) and total alkalin...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1006/phrs.1999.0486
更新日期:1999-08-01 00:00:00
abstract::Using cultured human keratinocytes, the present study investigates the role of nitric oxide (NO) in the mediation of the antiproliferative effects of ultraviolet light A (UVA). UVA treatment of cells (3-21 J cm (-2)) caused a time- and dose-dependent increase in nitrite formation in a micromolar range. This effect was...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1006/phrs.2001.0939
更新日期:2002-03-01 00:00:00
abstract::In hypothermic rats with acute hypertension induced by intravenous injection of adrenalin, regional changes in blood-brain barrier permeability to macromolecules were investigated using Evans blue as indication. Evans blue albumin extravasation was determined as a macroscopic finding and a quantitative estimation with...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:
更新日期:1992-07-01 00:00:00
abstract::Tachykinins are important in the development of pulmonary inflammation in mice but the tachykinin receptor subtype mediating this response has not been defined. To elucidate the role of tachykinin NK3-receptors on allergen-induced pulmonary inflammation, studies were performed on ovalbumin (OVA) sensitized and challen...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2004.07.002
更新日期:2004-12-01 00:00:00
abstract::Pharmacokinetics is a relatively young branch of the life sciences, which has developed rapidly in the last 15-20 years, thanks to a series of analytical achievements that have allowed drug concentrations to be measured in biological matrices with highly selective and sensitive methods. Most old drugs and all new drug...
journal_title:Pharmacological research
pub_type: 杂志文章,评审
doi:10.1006/phrs.1997.0254
更新日期:1997-12-01 00:00:00
abstract::It has been reported that patients with Hodgkin's disease (HD) show altered porphyrin metabolism, and suggested that the cause is the neoplastic process itself. If this is true, disease progression should be associated with higher levels of porphyrin excretion. The aim of this study was to evaluate urinary coproporphy...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2004.09.005
更新日期:2005-03-01 00:00:00
abstract::It is well known that type 2 diabetes mellitus is frequently associated with vascular dysfunction and an elevated systemic blood pressure, yet the underlying mechanisms are not completely understood. We previously reported that in mesenteric arteries from established type 2 diabetic Otsuka Long-Evans Tokushima fatty (...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2010.03.003
更新日期:2010-09-01 00:00:00
abstract::Capsaicin at a concentration of 10(-7)m induced a significant increase in heart rate and increased coronary flow in isolated Langendorff-perfused guinea-pig hearts. This effect was completely blocked by 30 microm of N(omega)-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthase. Additional incu...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1006/phrs.1999.0475
更新日期:1999-08-01 00:00:00
abstract::T lymphocytes produced by the thymus are essential mediators of immunity. Accelerated thymic atrophy appears in the patients with administration of glucocorticoids (GCs) which are commonly-used drugs to treat autoimmune and infectious diseases, leading to dysregulation of immunity with manifestation of progressive dim...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2018.09.011
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Thymidylate synthase (TS) expression levels appear to be related to response to 5-fluorouracil-(5-FU)-based chemotherapy in colorectal cancer (CRC) patients. Three polymorphisms have been proposed as modulators of TS expression: a tandemly repeated sequence (2R/3R) in the 5' UTR, a SNP (G>C) w...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2011.04.006
更新日期:2011-09-01 00:00:00
abstract::Regulatory T cells (Tregs) modulate ongoing immune responses to prevent autoimmunity in healthy bodies and inhibit effective anti-tumor immunity responses in tumor patients, leading to tumor progression. The function of Tregs in tumor immunity suggests that elimination of Tregs in the host may enhance the anti-tumor i...
journal_title:Pharmacological research
pub_type: 杂志文章,评审
doi:10.1016/j.phrs.2020.104659
更新日期:2020-03-01 00:00:00
abstract::Derivatization of phenothiazine (PTZ, 1) has been a commonly used method to develop drugs with various pharmacological properties. In the present study, a series of PTZ derivatives 1-11 were investigated on the inhibition of the cloned α7 subunit of the human nicotinic acetylcholine receptor (α7-nAChR) expressed in Xe...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2012.05.008
更新日期:2012-09-01 00:00:00
abstract::Perinatal encounter of a hormone and its developing target receptor sets the receptor-hormone-signal transduction complex for life (hormonal imprinting). In this critical period excess of the appropriate hormone or foreign molecules able to bind the receptor can cause faulty imprinting with life-long consequences. At ...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/s1043-6618(03)00217-2
更新日期:2003-11-01 00:00:00
abstract::Antifertility activity of a triterpenoid glycoside, DSS, isolated from the root of Dalbergia saxatilis was investigated in female Wistar rats of breeding age. When administered by gastric intubation at a dose rate of 200 mg kg(-1)body weight at the premating period, conception was inhibited in 71.4% of the treated ani...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1006/phrs.1999.0615
更新日期:2000-05-01 00:00:00
abstract::Segetoside I is a plant-derived bisdesmosidic saponin from Vaccaria segetalis (Neck) with reported anticancer activities. This development has raised an interest in the therapeutic potential of segetoside I. Here, we report the in vitro and in vivo antitumor activities of segetoside I against some selected cancer cell...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2016.04.032
更新日期:2016-08-01 00:00:00
abstract::Photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX may play a role in the treatment of dysplastic Barrett's oesophagus. An ALA thermosetting gel Pluronic F-127) was developed and evaluated in an in vivo mouse model for potential use in PDT of Barrett's mucosa. In vitro studies of th...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1006/phrs.2001.0934
更新日期:2002-02-01 00:00:00
abstract::We have shown previously that the antitumor drug, hydroxyurea (HU) is able to induce dose-dependent increases in plasma corticosterone levels in the rat. The drug was administered early in the morning, when the levels of circulating glucocorticoids are low in this species. Normally, in humans under physiological condi...
journal_title:Pharmacological research
pub_type: 临床试验,杂志文章
doi:10.1006/phrs.2000.0709
更新日期:2000-10-01 00:00:00
abstract::The gastrointestinal effects of single and repeated administration of ferrous sulphate was evaluated measuring faecal flora modifications and histology of stomach and duodenum of the rat. The acute experiments showed reversible histopathological lesions of stomach and duodenum with iron deposition and increase in faec...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1006/phrs.1993.1007
更新日期:1993-01-01 00:00:00
abstract::The effect of fluvoxamine (FLV) a selective serotonin reuptake inhibitor agent was studied on ouabain-induced arrhythmia in spontaneously beating isolated guinea-pig atria. FLV (8-64 microM) caused a dose-dependent decrease in the rate of contractions (7-52%) and in the contractile force (11-57%). Ouabain alone (2 mic...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2005.01.002
更新日期:2005-08-01 00:00:00
abstract::Many data suggest an involvement of toxic oxygen radicals in the termination of endurance to muscle fatigue. Being reduced glutathione (GSH), an efficient intracellular physiological antioxidant, experiments have been performed to discover whether exogenous GSH modifies endurance to exhaustive swimming in mice. GSH wa...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/s1043-6618(05)80116-1
更新日期:1991-02-01 00:00:00
abstract::Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by motor neurons degeneration and muscular atrophy. There is no effective SMA treatment. Loganin is a botanical candidate with anti-inflammatory, anti-oxidant, glucose-lowering and anti-diabetic nephropathy activities. The ...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2016.05.023
更新日期:2016-09-01 00:00:00
abstract::The cytoprotective activity of alpha-lipoic acid against free radical toxicity, manifested during experimental hyperoxaluria, has been investigated. Glycollate was used as the inducer of oxalate hyperoxaluria in rats. The increase in lipid peroxidation and superoxide dismutase (SOD) activity, associated with a decreas...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1006/phrs.1993.1031
更新日期:1993-05-01 00:00:00