Abstract:
:Diabetes is associated with a high level of mortality due to cardiovascular disease resulting from accelerated coronary artery atherosclerosis. A current focus for investigation of atherosclerotic mechanisms is the vascular endothelium since physical or functional injury may represent an initiating step for atherogenesis. Thiazolidinediones (TZDs) are the newest class of drugs for the treatment of insulin resistance and its metabolic consequences; they are peroxisome proliferator-activating receptor (PPAR)-gamma ligands that act as insulin-sensitizing agents. We are interested in the contribution of direct vascular actions to the clinical utility of these agents. We investigated the effect troglitazone and rosiglitazone on endothelial cell proliferation in low- and high-glucose media and further explored their action on the ubiquitous membrane transport system, the Na/H exchanger (NHE), which has been implicated in regulating the growth of vascular cells. Experiments were conducted in cultured bovine aortic endothelial cells (BAECs). Cell proliferation was assessed by cell counting, and NHE activity was determined in cells loaded with the pH-sensitive fluorescent dye, 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester (BCECF-AM). Troglitazone caused a dose-dependent inhibition of endothelial cell proliferation with approximately 50% inhibition at 10 microM. Troglitazone inhibited endothelial cell proliferation with similar potency under low- (5 mM) and high-glucose (25 mM) concentrations. Rosiglitazone had no significant effect on endothelial cell proliferation at concentrations of up to 100 microM under low- or high-glucose concentrations. The NHE inhibitor, 3-metlylsulfonyl-4-piperidinobenzoyl guanidine (HOE 694), caused dose dependent inhibition of BAEC proliferation, which was independent of the media glucose concentration. Acute exposure of cells to troglitazone (10 microM) and rosiglitazone (30 microM) during recovery from acidosis showed slight but significant (P<.05) inhibition of NHE activity by troglitazone, but no significant (P>.05) effect by rosiglitazone. Exposure of cells to either drug for 24 h revealed no chronic regulation of NHE activity. Our data demonstrate that troglitazone has similar actions in endothelial cells as in vascular smooth muscle. The absence of rosiglitazone effects, a more potent PPAR-gamma activator, suggests that the observed actions of troglitazone may be at least partially independent of PPAR-gamma. The effects of troglitazone and rosiglitazone on endothelial cell proliferation and NHE activity, although contrasting, are consistent with a central signalling role of this transporter in cell proliferation.
journal_name
J Diabetes Complicationsjournal_title
Journal of diabetes and its complicationsauthors
de Dios ST,Hannan KM,Dilley RJ,Hill MA,Little PJdoi
10.1016/s1056-8727(01)00141-6keywords:
subject
Has Abstractpub_date
2001-05-01 00:00:00pages
120-7issue
3eissn
1056-8727issn
1873-460Xpii
S1056-8727(01)00141-6journal_volume
15pub_type
杂志文章abstract:AIMS:The present study investigated the association of serum levels of angiopoietin-related growth factor (AGF) with lower extremity peripheral arterial disease (LEPAD). METHODS:The study group is comprised of 105 patients with lower extremity peripheral arterial disease. The control group consisted of 80 individuals ...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2016.10.019
更新日期:2017-02-01 00:00:00
abstract::We examined the potential of astemizole, a histamine H1-receptor antagonist that does not cross the blood-brain barrier, to reverse blood-retinal barrier leakage to albumin in streptozotocin diabetic rats. Four groups of nondiabetic and four groups of diabetic rats received vehicle or astemizole at dosages of 5, 10, o...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/1056-8727(92)90057-r
更新日期:1992-10-01 00:00:00
abstract:AIMS:The purpose of this study was to investigate the impact of diabetic neuropathy (dNP) on lower limb endurance, explosive and maximal muscle strength in patients with Type 2 Diabetes Mellitus (T2DM). METHODS:Fifty-four participants, aged between 55 and 85, were enrolled in this observational comparative study. The ...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2020.107562
更新日期:2020-06-01 00:00:00
abstract:AIMS:Type 2 diabetes (T2DM) is associated with cognitive impairment. We examined whether undiagnosed cognitive impairment in T2DM-patients is associated with a reduced health status and depressive symptoms. METHODS:In an observational study, 225 T2DM-patients aged ≥70years were examined at their homes and (some of the...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2015.07.010
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVE:To characterize acute (postprandial) and chronic (after a 6-month period of weight loss) effects of a low-carbohydrate vs. a low-fat diet on subclinical markers of cardiovascular disease (CVD) in adults with type 2 diabetes. DESIGN:At baseline and 6 months, measures of C-reactive protein (CRP), interleukin-6...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jdiacomp.2011.08.001
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:Current approaches to the management of type 2 diabetes focus on the early initiation of novel pharmacologic therapies and bariatric surgery. OBJECTIVE:The purpose of this study was to revisit the use of intensive, outpatient, behavioral weight management programs for the management of type 2 diabetes. DES...
journal_title:Journal of diabetes and its complications
pub_type: 临床试验,杂志文章
doi:10.1016/j.jdiacomp.2014.03.014
更新日期:2014-07-01 00:00:00
abstract:UNLABELLED:Controversial data suggest that patients with type 2 diabetes mellitus have an increased risk of fractures despite having, in some studies, higher bone mineral density. METHODS:The aim of this study was to determine the prevalence of osteoporosis and morphometric vertebral fractures in 148 postmenopausal di...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2011.02.004
更新日期:2011-07-01 00:00:00
abstract:AIM:To evaluate the use of the FINDRISC score in an overweight and obese population to predict glucose status. METHODS:In 651 overweight/obese subjects (M/F: 193/458, age 43±13 y, BMI 38.2±6.1kg/m(2)) glucose status was tested using OGTT and HbA1c. Furthermore, the FINDRISC questionnaire and CT visceral fat (VAT) and ...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2016.05.004
更新日期:2016-08-01 00:00:00
abstract:BACKGROUND:In this study we evaluated the association of baseline levels of six different candidate proteins for the development of microalbuminuria and macroalbuminuria in type 1 diabetic patients, who were followed for approximately 30 years. Two of the proteins are markers of inflammation: serum amyloid A (SAA) and ...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2012.06.016
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:The prevalence of the metabolic syndrome is rapidly increasing with a considerable ethnic variation within and across populations. This study was conducted to estimate the prevalence of the metabolic syndrome and its individual components using Adult Treatment Panel III (ATP III) criteria among Northern Jord...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2005.11.003
更新日期:2007-07-01 00:00:00
abstract::Hypertension and diabetes mellitus are independently associated with a high rate of target organ complications, which is particularly accentuated in the Negroid race. The aims of this study were to evaluate the mortality associated with diabetes mellitus and concurrent hypertension and diabetes mellitus in indigenous ...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/s1056-8727(00)00069-6
更新日期:2000-03-01 00:00:00
abstract:BACKGROUND:The use of safe iodinated contrast media (CM) to prevent contrast-induced nephropathy (CIN) is an important consideration among renally impaired diabetic patients during coronary angiography. HYPOTHESIS:Diabetic patients with normal or mild renal dysfunction are less likely to receive renal protective measu...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jdiacomp.2006.11.002
更新日期:2008-05-01 00:00:00
abstract::With the rise in childhood obesity, type 2 diabetes mellitus (T2DM) has been recognized to occur in adolescents with increasing frequency. Although much is known about T2DM in adults, few studies have examined the treatment and complications of T2DM in youth. The Treatment Options for type 2 Diabetes in Adolescents an...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章,评审
doi:10.1016/j.jdiacomp.2014.10.009
更新日期:2015-03-01 00:00:00
abstract::The thickening of alveolar basement membrane is found in autopsies, along with microvascular pathologies, in Type 1 and 2 diabetes mellitus (DM). To detect the function and permeability of alveolar basement membrane, carbon monoxide diffusion capacity (DLCO) and technetium 99m-diethyltriaminepentaaceticacid ((99m)Tc-D...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2005.07.003
更新日期:2006-07-01 00:00:00
abstract:AIMS:To develop and validate prediction equations to identify individuals at high risk for type 2 diabetes using existing health plan data. METHODS:Health plan data from 2005 to 2009 from 18,527 members of a Midwestern HMO without diabetes, 6% of whom had fasting plasma glucose (FPG) ≥110mg/dL, and health plan data fr...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2013.01.003
更新日期:2013-11-01 00:00:00
abstract:AIMS:To estimate short-term mortality rates for individuals with type 1 or type 2 diabetes diagnosed before age 20 years from the SEARCH for Diabetes in Youth study. METHODS:We included 8358 individuals newly-diagnosed with type 1 (n = 6840) or type 2 (n = 1518) diabetes from 1/1/2002-12/31/2008. We searched the Natio...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章,多中心研究
doi:10.1016/j.jdiacomp.2018.03.015
更新日期:2018-06-01 00:00:00
abstract:AIMS:Using psychological and quality of life assessment tools, we prospectively studied changes in health-related quality of life and emotional well-being in patients who had commenced GLP-1 analogue therapy (exenatide) and compared them with new insulin starters. METHODS:Two matched groups of patients with type 2 dia...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2011.03.002
更新日期:2011-07-01 00:00:00
abstract:AIM:To evaluate the efficacy and safety of iGlarLixi in Mexican patients with type 2 diabetes who participated in the LixiLan clinical trials and compare results with the rest of the patients. METHODS:Data was collected for Mexican patients who participated in either of three studies: phase 2 trial LixiLan-POC, that c...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2019.05.020
更新日期:2020-08-01 00:00:00
abstract:AIMS:Diabetic peripheral neuropathy (DPN) accompanied by painful symptoms is known as painful DPN (pDPN). This study characterized healthcare resource utilization and costs in patients with DPN, pDPN, and severe pDPN relative to diabetes only. METHODS:Four adult cohorts were identified from the Humedica database: type...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2014.10.013
更新日期:2015-03-01 00:00:00
abstract::Distal symmetric peripheral polyneuropathy (DSPN) is a highly prevalent complication of diabetes. However, underlying pathophysiological mechanisms are multiple and not well understood. The aim of our study was to analyze the oxidative stress levels in circulating mononuclear cells by measuring the glutathione system,...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2015.05.023
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND:Glomerular hyperfiltration is not able to be detected in clinical practice. We assessed whether hyperfiltration is associated with albuminuria progression among Indigenous Australians at high risk of diabetes and kidney disease to determine its role in kidney disease progression. METHODS:Longitudinal observ...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2019.02.005
更新日期:2019-05-01 00:00:00
abstract::We randomized 102 patients with insulin-dependent diabetes to intensified treatment (n = 48 at baseline, n = 42 after 7.5 years) or standard treatment (n = 54 at baseline, n = 47 after 7.5 years). As has previously been reported, intensified treatment resulted in a retardation of retinopathy, nephropathy, and neuropat...
journal_title:Journal of diabetes and its complications
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/1056-8727(94)00008-c
更新日期:1995-01-01 00:00:00
abstract:AIMS:To investigate TAp63 expression in patients with type 2 diabetes mellitus (T2DM) and the potential correlations between TAp63 and proinflammatory cytokines production and other clinical parameters. METHODS:Peripheral blood mononuclear cells (PBMCs) and plasma were collected from 72 T2DM (cases) and 72 healthy sub...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2017.12.013
更新日期:2018-03-01 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) significantly increases morbidity and mortality from cardiovascular disease (CVD). Treatments for patients with T2DM have the potential to reduce cardiovascular (CV) risk. This review focuses on the potential of a new class of antidiabetic agents, the sodium glucose cotransporter 2 (SGL...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章,评审
doi:10.1016/j.jdiacomp.2012.12.004
更新日期:2013-05-01 00:00:00
abstract:OBJECTIVE:Two insulin regimens were used to explore acute and chronic postprandial changes in glycemia, lipemia, and metabolic markers associated with increased risk of cardiovascular disease. METHODS:An open-label, randomized, two-period crossover study (12 weeks/period) compared a prandial regimen [premeal insulin l...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jdiacomp.2005.11.005
更新日期:2007-01-01 00:00:00
abstract:AIM:Investigate contributors to treatment satisfaction in type 1 diabetes (T1D). METHODS:Post-hoc analysis using the Diabetes Treatment Satisfaction Questionnaire status version (DTSQs) in 771 T1D patients from two 28-week trials comparing once-daily insulin glargine 100U/mL (Gla-100) with once- or twice-daily NPH neu...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2016.11.018
更新日期:2017-03-01 00:00:00
abstract:OBJECTIVE:This study was to observe the difference in one-hour postload plasma glucose levels and analyze its related factors in abdominally obese men with normal glucose tolerance (NGT). DESIGN:This case-control study included 36 abdominally obese men (waist circumference ≥90 cm) and 31 non-abdominally obese men (wai...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/j.jdiacomp.2012.05.022
更新日期:2012-11-01 00:00:00
abstract::Paecilomyces is a saprophytic soil fungus that is an uncommon human pathogen. We report a diabetic patient who developed an upper lobe pulmonary infection due to Paecilomyces varioti. This pneumonia responded poorly to oral imidazole therapy with ketoconazole. Eventual treatment of the chronic infiltrating process req...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/1056-8727(92)90027-i
更新日期:1992-04-01 00:00:00
abstract::Intact lenses from New Zealand white rabbits were incubated in tissue culture media containing either 5 mM glucose or 5 mM glucose plus 30 mM galactose. The standard media did not contain taurine. Lenses were also cultured in a third medium containing 30 mM galactose plus 0.2 mM taurine. The frequency of cataract form...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章
doi:10.1016/1056-8727(93)90023-r
更新日期:1993-01-01 00:00:00
abstract::Although slow gastric emptying (gastroparesis) is a well-known complication of chronic hyperglycemia in diabetes mellitus (DM), it recently has become clear that rapid gastric emptying also is a frequent and important diabetic complication. In contrast, acute hyperglycemia causes slow gastric emptying, and acute hypog...
journal_title:Journal of diabetes and its complications
pub_type: 杂志文章,评审
doi:10.1016/j.jdiacomp.2019.107414
更新日期:2019-11-01 00:00:00