Abstract：
:The co-ordinated glucuronidation and export of compounds from cells is an important determinant in the detoxification of many compounds in vivo. Many UDP-glucuronosyltransferases (UGTs) and several multidrug resistance proteins (MRPs) have been cloned and individually expressed to assess specificity of glucuronidation and transport. However, to further understand the interplay between glucuronidation and transport we are developing stable cell lines that express different combinations of UGT and MRP isoforms. V79 cells expressing both UGT1A1 and MRP1 have been established. The ability of these cell lines to both glucuronidate and transport compounds was assessed ex vivo using estradiol and bilirubin as substrates.
journal_name
Toxicol Lettjournal_title
Toxicology lettersauthors
Cuff RL,Wade LT,Rychlik B,Jedlitschky GA,Burchell Bdoi
10.1016/s0378-4274(01)00305-8keywords:
subject
Has Abstractpub_date
2001-03-31 00:00:00pages
43-9issue
1-3eissn
0378-4274issn
1879-3169pii
S0378-4274(01)00305-8journal_volume
120pub_type
杂志文章abstract：:Inflammation is one of the factors that may increase the sensitivity of hepatic cells to acetaminophen (APAP) induced toxicity. To investigate the mechanisms, we exposed 3-dimensional (3D) Human Liver Microtissues, a co-culture of primary human hepatocytes (PHH) and Kupffer cells (KCs), to 0, 0.5 (low), 5 (median) and...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2019.05.004
更新日期：2019-09-15 00:00:00
abstract：:N-acetyl-beta-D-glucosaminidase (NAG) activity in urine was measured in patients with 'itai-itai' disease, from chronically advanced cadmium poisoning. Elevation of NAG activity, however, was not so marked as that of beta 2-microglobulin (beta 2-MG) in the urine of patients and suspected patients. We conclude that the...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(83)90193-5
更新日期：1983-05-01 00:00:00
abstract：:Furan, a potential human carcinogen, is formed during heat-treatment of food. Previous studies of the genotoxicity of furan have given disparate results. Hence, there is a need for complementary data to clarify the mechanism behind the carcinogenicity of furan. In this study, we have used the flow cytometer-based micr...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2006.08.020
更新日期：2007-02-28 00:00:00
abstract：:Toxic and volatile chemicals are widely used in household products and previously used as warfare agents, causing a public health threat worldwide. This study aimed to evaluate the extent of injury and mechanisms of acrolein toxicity in the cornea. Primary human corneal stromal fibroblasts cultures (hCSFs) from human ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2021.01.005
更新日期：2021-01-06 00:00:00
abstract：:Several perfluoroalkyl compounds (PFCs) are ubiquitous environmental contaminants that can biomagnify in species at high trophic levels including wild birds. Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) have been detected in wild birds and are known to reduce hatching success of laboratory-expose...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2009.07.004
更新日期：2009-10-28 00:00:00
abstract：:Exercising volunteers exposed in chambers to as little as 80 ppb O3 for several hours exhibit impaired lung function and irritative lower airway symptoms. Comparable changes occur among children and young adults exposed to summer smog containing O3. Intensity of the response is reproducible but varies widely among ind...
journal_title：Toxicology letters
pub_type： 杂志文章,评审
doi：10.1016/0378-4274(95)03565-6
更新日期：1995-12-01 00:00:00
abstract：:5-Azactydine inhibits cell growth by direct cytotoxic action as well as by inhibition of DNA methyl transferase enzyme. Inhibitors of DNMT have been reported to potentiate the therapeutic activity of cisplatin in vitro. Dose dependent bone marrow toxicity, neurotoxicity and nephrotoxicity are the major side effects of...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2009.08.018
更新日期：2009-12-15 00:00:00
abstract：:The necessity to protect humans from the adverse effects of chemicals on structure and function of the developing and/or mature nervous system is increasingly recognized among regulatory bodies throughout the world. Whereas structural changes dominated much of neurotoxicological research in the past, functional marker...
journal_title：Toxicology letters
pub_type： 杂志文章,评审
doi：10.1016/0378-4274(92)90195-p
更新日期：1992-12-01 00:00:00
abstract：:The effects of vitamin C on acute paracetamol poisoning were studied in mice. Given orally at 50, 100 or 200 mg/kg, either simultaneously or 2 h after the oral administration of 875 mg/kg of paracetamol, vitamin C did not cause any decrease in the mortality rate. In similar conditions in previous experiments methionin...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(83)90086-3
更新日期：1983-08-01 00:00:00
abstract：:Neurological complications as well as movement disorders are relevant symptoms in animals and humans chronically exposed to dithiocarbamates. Using rat pheochromocytoma cells differentiated by NGF (PC12), we investigated whether propineb affects acetylcholine (Ach) release and the molecular mechanisms involved. Propin...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2008.08.016
更新日期：2008-11-10 00:00:00
abstract：:Increasing evidences suggest that microcystins, a kind of toxic metabolites, produced by cyanobacteria in contaminated water may contribute to the aggravation of the human colorectal carcinoma. Our previous study showed that microcystin-LR (MC-LR) exposure caused significant invasion and migration of colorectal cancer...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2016.11.004
更新日期：2017-01-04 00:00:00
abstract：:The toxicity of diethylene glycol butyl ether (DGBE), and its principal metabolite, butoxyethoxyacetic acid (BEAA), were assessed in vitro for rat and human red blood cells. Rat erythrocytes showed evidence of mild hemolysis when exposed to BEAA at concentrations of 5 or 10 mM for 4 h. BEAA treated rat red blood cells...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2003.09.021
更新日期：2005-03-28 00:00:00
abstract：:Because coarse mode particles are rarely studied in their existing size ranges (greatest mass about 5-7 microns, aerodynamic diameter), we investigated the effects of four such particles, quartz, ferric oxide, calcium carbonate, and sodium feldspar, on host defenses against bacterial pulmonary infection. Mice which re...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(83)90263-1
更新日期：1983-10-01 00:00:00
abstract：:Alkyl hydroperoxide reductase 1 (Ahp1p) is a thioredoxin peroxidase of the peroxiredoxin family expressed by Saccharomyces cerevisiae. Recently, disruption of the AHP1 gene has shown that the gene is not essential for yeast growth on glucose medium but revealed a high sensitivity of null mutants to organic peroxides, ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(02)00280-1
更新日期：2002-10-05 00:00:00
abstract：:Effect of chrysotile dust alone or together with Candida albicans administered intratracheally in guinea pigs was studied in the genesis of pleural plaques over a period of 12 months. A significant increase of mucopolysaccharides, phosphorus, calcium and -SH content was detected in pleural fluid of animals treated wit...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(82)90207-7
更新日期：1982-10-01 00:00:00
abstract：:Trihalomethanes (THMs; chloroform, bromoform, bromodichloromethane, dibromochloromethane), formed as by-products of chlorine disinfection, are found to occur in combination in drinking water supplies. THMs are metabolized by cytochromes P-450 and are likely substrates of CYP2E1. Therefore, it is possible that mixed ex...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(99)00022-3
更新日期：1999-05-20 00:00:00
abstract：:Guidelines concerning the preclinical safety assessment of therapeutic proteins are available, e.g. in the European Community (EC Notes for Guidance). Unlike rather rigidly prescribing test systems such as those known for chemical substances, it is agreed that for biotechnologically-produced products, testing may be q...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(92)90206-y
更新日期：1992-12-01 00:00:00
abstract：:Cyanotoxins are now recognised by international and national health and environment agencies as significant health hazards. These toxins, and the cells which produce them, are also vulnerable to exploitation for illegitimate purposes. Cyanotoxins are increasingly being subjected to national and international guideline...
journal_title：Toxicology letters
pub_type： 杂志文章,评审
doi：10.1016/j.toxlet.2006.01.011
更新日期：2006-05-25 00:00:00
abstract：INTRODUCTION:An increased health problem in industrialised countries is the contemporary concern of public and scientific community as well. This has been attributed in part to accumulated environmental pollutants especially radioactive substances and the use of nuclear power plants worldwide. However, the outcome of c...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2017.10.004
更新日期：2018-01-05 00:00:00
abstract：:When pregnant mice were exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the average time to eye opening in the offspring was shortened by about a day. How acceleration of eye opening by TCDD occurs remains unknown. To reveal the underlying mechanisms of the accelerated eye opening, pregnant mice were intraperit...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2010.04.001
更新日期：2010-07-01 00:00:00
abstract：:Localization of specific mRNA and protein molecules within cells and tissues can provide important information on the effects of a drug within a biological test system and help elucidate mechanisms of drug-induced toxicity and organ dysfunction. The most widely used techniques for measuring the cellular and tissue dis...
journal_title：Toxicology letters
pub_type： 杂志文章,评审
doi：10.1016/s0378-4274(99)00204-0
更新日期：2000-03-15 00:00:00
abstract：:Vincristine (VCR) is commonly used to treat a variety of hematological malignancies and solid tumors in pediatric and adult patients. However, peripheral neuropathy is a dose-limiting side effect that leaves some patients with functional disability and long-term pain. Oxytocin (OT) has demonstrated analgesic and anti-...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2021.01.008
更新日期：2021-01-08 00:00:00
abstract：:The effects of cadmium (Cd) on the immune organs were examined histopathologically. On 2 or 3 days after a single i.p. injection of 1.8 mg Cd/kg body weight into mice, slight loss of body weight, significant decrease of thymus weight and marked increase of spleen weight were observed. Lymph node weight did not show an...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(81)90137-5
更新日期：1981-04-01 00:00:00
abstract：:Mouse neuroblastoma cells exposed to 2.5 and 5.0 microM methylmercury for 24 h appeared rounded with the loss of processes. Immunohistochemical staining directed against beta-tubulin revealed severe alterations in microtubular architecture. Non-membrane-bound condensation product was visualized ultrastructurally in th...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(90)90226-c
更新日期：1990-03-01 00:00:00
abstract：:20 non-smokers on a defined diet low in polycyclic aromatic hydrocarbons (PAH) were exposed to environmental tobacco smoke (ETS) in an unventilated room for 8 h. The urinary mutagenicity in the 24-h urine samples as tested with the Salmonella (TA98) microsome assay did not significantly increase after exposure to eith...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(87)90098-1
更新日期：1987-01-01 00:00:00
abstract：:The effects of selenium on cellular toxicity and histochemical distribution of mercury were examined in a cell culture system of mouse peritoneal macrophages. Selenium protected against the toxicity of mercury in cultures exposed to 4 microM of mercuric chloride. Selenomethionine caused a significant increase in cell ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(89)90144-6
更新日期：1989-06-01 00:00:00
abstract：:Chloroform-induced toxicity in rodents depends on oral dose regimen. We evaluated the absorption and tissue dosimetry of chloroform after gavage administration in various vehicles to male Fischer 344 rats and female B6C3F1 mice. Animals received a single dose of chloroform in corn oil, water, or aqueous 2% emulphor at...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(97)00023-4
更新日期：1997-05-16 00:00:00
abstract：:Male C57B1/6N mice, 8-10 weeks old were given a single oral dose of 0, 1.0 or 3.0 mg/kg body weight of trimethyltin hydroxide (TMT). Levels of ornithine decarboxylase (ODC) activity were measured in several brain areas, 1, 2 and 7 days later. The lower dose of TMT produced a decrease of ODC in the caudate nucleus and ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(87)90042-7
更新日期：1987-03-01 00:00:00
abstract：:With the multitude of new chemicals being synthesized and the paucity of long-term test data on chemicals that could be introduced into the environment, innovative approaches must be developed to determine the health and environmental effects of chemicals. Research was conducted to employ quantitative structure-activi...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(95)03365-r
更新日期：1995-09-01 00:00:00
abstract：:Hydrochlorofluorocarbon 1,1-dichloro-2,2,2-trifluoroethane (HCFC-123), a close structural analogue of the hepatotoxic anaesthetic halotane and a replacement for some ozone-depleting chlorofluorocarbons, is metabolized by liver cytochrome P450 (P450), both in vitro and in vivo. P450 activates HCFC-123, both oxidatively...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(03)00232-7
更新日期：2003-09-15 00:00:00
