Abstract：
:The behavior of chromosomal inversions in Escherichia coli depends upon the region they affect. Regions flanking the replication terminus have been termed nondivisible zones (NDZ) because inversions ending in the region were either deleterious or not feasible. This regional phenomenon is further analyzed here. Thirty segments distributed between 23 and 29 min on the chromosome map have been submitted to an inversion test. Twenty-five segments either became deleterious when inverted or were noninvertible, but five segments tolerated inversion. The involvement of polar replication pause sites in this distribution was investigated. The results suggest that the Tus/pause site system may forbid some inversion events, but that other constraints to inversion, unrelated to this system, exist. Our current model for deleterious inversions is that the segments involved carry polar sequences acting in concert with other polar sequences located outside the segments. The observed patchwork of refractory and tolerant segments supports the existence of several NDZs in the 23- to 29-min region. Microscopic observations revealed that deleterious inversions are associated with high frequencies of abnormal nucleoid structure and distribution. Combined with other information, the data suggest that NDZs participate in the organization of the terminal domain of the nucleoid.
journal_name
Geneticsjournal_title
Geneticsauthors
Guijo MI,Patte J,del Mar Campos M,Louarn JM,Rebollo JEkeywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
1413-23issue
4eissn
0016-6731issn
1943-2631journal_volume
157pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Reversion from the suppressed to nonsuppressed phenotype in strains of geno;type SUQ5 [psi+] ade2-1 his5-2 lys1-1 can1-100 ura3-1 has been induced by treatment with ethyl methanesulphonate, nitrosoguanidine or UV (254 nm) light. Spontaneously occurring revertants have also been selected by two different methods. Rever...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1980-07-01 00:00:00
abstract：:Transmission-ratio distortion is a departure from a 1:1 segregation of alleles in the gametes of a heterozygous individual. The so-called driving allele is strongly selected regardless of its effect on the fitness of the carrying individual. It may then have an important impact on neutral polymorphism due to the genet...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.052977
更新日期：2006-07-01 00:00:00
abstract：:SNAP receptor (SNARE) and Sec1/Munc18 (SM) proteins are required for all intracellular membrane fusion events. SNAREs are widely believed to drive the fusion process, but the function of SM proteins remains unclear. To shed light on this, we screened for dominant-negative mutants of yeast Sec1 by random mutagenesis of...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.090423
更新日期：2008-09-01 00:00:00
abstract：:The B-Peru allele of the maize b regulatory gene is unusual relative to most b alleles in that it is expressed in the aleurone layer of the seed. It is also expressed in a subset of plant vegetative tissues. Transgenic maize plants containing the B-Peru gene with the first 710 bases of upstream sequence conferred the ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
abstract：:Plants with mutations in one of three maize genes, mop1, rmr1, and rmr2, are defective in paramutation, an allele-specific interaction that leads to meiotically heritable chromatin changes. Experiments reported here demonstrate that these genes are required to maintain the transcriptional silencing of two different tr...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.058669
更新日期：2006-07-01 00:00:00
abstract：:The consequences of asymmetric dispersion on the maintenance of an allele in a one-dimensional environmental pocket are examined. The diffusion model of migration and selection is restricted to a single diallelic locus in a monoecious population in the absence of mutation and random drift. It is further supposed that ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1978-04-01 00:00:00
abstract：:Cyclic guanosine monophosphate (cGMP) is a key secondary messenger used in signal transduction in various types of sensory neurons. The importance of cGMP in the ASE gustatory receptor neurons of the nematode Caenorhabditis elegans was deduced by the observation that multiple receptor-type guanylyl cyclases (rGCs), en...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.152660
更新日期：2013-08-01 00:00:00
abstract：:The knotted1 gene was first defined by dominant mutations that affect leaf morphology. The original allele, Kn1-O, results from a 17-kb tandem duplication. Mutator (Mu) insertions near the junction of the two repeats suppress the leaf phenotype to different degrees depending on the position of the insertion. The Mu in...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Mitotic cell divisions increase cell number while faithfully distributing the replicated genome at each division. The Caenorhabditis elegans embryo is a powerful model for eukaryotic cell division. Nearly all of the genes that regulate cell division in C. elegans are conserved across metazoan species, including humans...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.118.301367
更新日期：2019-01-01 00:00:00
abstract：:Next generation sequencing (NGS) is about to revolutionize genetic analysis. Currently NGS techniques are mainly used to sequence individual genomes. Due to the high sequence coverage required, the costs for population-scale analyses are still too high to allow an extension to nonmodel organisms. Here, we show that NG...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.114397
更新日期：2010-09-01 00:00:00
abstract：:l(3)S12 is a vital locus whose function is required in embryos, early larvae, late pupae and oogenesis. We have identified a cold-sensitive allele, l(3)S12(3), and characterized conditional misexpression of the gene associated with this mutation as well as with several euchromatic insertions of l(3)S12+ transposons. S...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:Two independent isolates of Saccharomyces cerevisiae lacking hexokinase activity (EC 2.7.1.1) are described. Both mutant strains grow on glucose but are unable to grow on fructose, and contain two mutant genes hxk1 and hxk2 each. The mutations are recessive and noncomplementing. Genetic analysis suggests that these tw...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-08-01 00:00:00
abstract：:The fission yeast DNA repair gene rad21+ is essential for cell growth. To investigate the function essential for cell proliferation, we have isolated a temperature-sensitive mutant of the rad21+ gene. The mutant, rad21-K1, showed abnormal mitosis at the nonpermissive temperature. Some cells contained abnormal nuclear ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:In Saccharomyces cerevisiae, genes located at the telomeres and the HM loci are subject to transcriptional silencing. Here, we report results of screening a Gal4 DNA-binding domain hybrid library for proteins that cause silencing when targeted to a silencer-defective HMR locus. ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.166.1.631
更新日期：2004-01-01 00:00:00
abstract：:Acrylamide gel electrophoresis was performed on the enzyme xanthine dehydrogenase in sixty isochromosomal lines of Drosophila persimilis from three geographic populations. Sequential electrophoretic analysis using varied gel concentrations and buffers revealed twenty-three alleles in this species where only five had b...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:Retroviruses and their relatives, the long terminal repeat (LTR) retrotransposons, carry out complex life cycles within the cells of their hosts. We have exploited a collection of gene deletion mutants developed by the Saccharomyces Genome Deletion Project to perform a functional genomics screen for host factors that ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：:In both pedigree linkage studies and in population-based association studies there has been much interest in the use of modern dense genetic marker data to infer segments of gene identity by descent (ibd) among individuals not known to be related, to increase power and resolution in localizing genes affecting complex ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.137570
更新日期：2012-04-01 00:00:00
abstract：:An algorithm for detecting local clusters of homologous genes was applied to the genome of Caenorhabditis elegans. Clusters of two or more homologous genes are abundant, totaling 1391 clusters containing 4607 genes, over one-fifth of all genes in C. elegans. Cluster genes are distributed unevenly in the genome, with t...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.040030
更新日期：2006-01-01 00:00:00
abstract：:We have studied how a set of male-specific sensory neurons in Caenorhabditis elegans establish axonal connections during postembryonic development. In the adult male, 9 bilateral pairs of ray sensory neurons innervate an acellular fan that serves as a presumptive tactile and olfactory organ during copulation. We visua...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.057000
更新日期：2006-07-01 00:00:00
abstract：:The change in the frequency of a rare mutant allele under constant sex-differentiated viability selection in an infinite, partial full-sib mating population is studied. The diplo-diploid and haplo-diploid polygynous models are considered with a Poisson distribution for the number of offspring produced by every mated f...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.039024
更新日期：2005-09-01 00:00:00
abstract：:Polyhomeotic is a member of the Polycomb group (Pc-G) of homeotic repressors. The proteins encoded by the Pc-G genes form repressive complexes on the polycomb group response element sites. The phP1 mutation was induced by insertion of a 1.2-kb P element into the 5' transcribed nontranslated region of the proximal poly...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:Dopamine acts through two classes of G protein-coupled receptor (D1-like and D2-like) to modulate neuron activity in the brain. While subtypes of D1- and D2-like receptors are coexpressed in many neurons of the mammalian brain, it is unclear how signaling by these coexpressed receptors interacts to modulate the activi...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.128512
更新日期：2011-07-01 00:00:00
abstract：:Neural ganglia of wild type third-instar larvae of Drosophila melanogaster were incubated for 13 hours at various concentrations of BUdR (1, 3, 9, 27 micrograms/ml). Metaphases were collected with colchicine, stained with Hoechst 33258, and scored under a fluorescence microscope. Metaphases in which the sister chromat...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-02-01 00:00:00
abstract：:The Aspergillus nidulans rcoADelta mutant exhibits growth and developmental defects. We show that the rcoADelta mutant lacks cleistothecia and is self-sterile. In crosses with wild-type strains, rcoADelta nuclei do not contribute to the cleistothecial walls. Furthermore, sexual development resulting from veA overexpre...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.062893
更新日期：2006-11-01 00:00:00
abstract：:Genes at the SerH locus of the ciliated protist Tetrahymena thermophila specify the major (H) surface protein on cells grown at 20-36 degrees. Alternative proteins L, T, S and I are expressed under different conditions of temperature and culture media. Mutants unable to express SerH genes were examined for expression ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:One of the most important parameters in population genetics is theta = 4Ne mu where Ne is the effective population size and mu is the rate of mutation per gene per generation. We study two related problems, using the maximum likelihood method and the theory of coalescence. One problem is the potential improvement of a...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:The TATA-binding protein (TBP) is composed of a highly conserved core domain sufficient for TATA-element binding and preinitiation complex formation as well as a highly divergent N-terminal region that is dispensable for yeast cell viability. In vitro, removal of the N-terminal region domain enhances TBP-TATA associat...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：:Neurons and glia display remarkable morphological plasticity, and remodeling of glia may facilitate neuronal shape changes. The molecular basis and control of glial shape changes is not well understood. In response to environmental stress, the nematode Caenorhabditis elegans enters an alternative developmental state, ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.137786
更新日期：2012-04-01 00:00:00
abstract：:A semi-lethal, sterile allele of the smooth locus (2-91.5), sm3, was discovered in an artificial selection line for low abdominal bristle number that had been started from a P-M dysgenic cross. The fitness effects and extremely low bristle number phenotype of the allele could not be separated by recombination from a P...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
abstract：:In Saccharomyces cerevisiae, the Rad52 protein plays a role in both RAD51-dependent and RAD51-independent recombination pathways. We characterized a rad52 mutant, rad52-329, which lacks the C-terminal Rad51-interacting domain, and studied its role in RAD51-independent recombination. The rad52-329 mutant is completely ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
