Abstract：
:Geminiviruses are single-stranded DNA plant infecting viruses that cause major losses in important crops in tropical and subtropical countries. Tomato leaf curl virus (TLCV) belonging to the genera Begomovirus, is a whitefly-transmitted geminivirus that causes a severe leaf curl disease in tomato (Lycopersicon esculentum). The importance of this disease has prompted a great need for a rapid identification of TLCV in its hosts and vector. Polymerase chain reaction (PCR) is the most sensitive approach to detect a minute amount of viral nucleic acid. It is the most ideal method to amplify geminiviruses as they replicate via a double-stranded, circular DNA form. In this study, geminivirus specific degenerated primers were employed to detect TLCV occurring in its vector whitefly Bemisia tabaci by PCR based approach. One primer pair, amplified TLCV DNA fragment of about 1.1 Kb representing partly replicase gene, intergenic region and partly coat protein gene was used. When a set of primer targeted to the core region of the coat protein gene of geminivirus was used, a PCR amplified fragment of about 0.5 Kb was obtained. This approach is highly useful for an early detection of TLCV occurring in very small amount in the vector B. tabaci. Its implications in geminivirus management strategies and their differentiation and being discussed.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Khan JAkeywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
512-5issue
5eissn
0019-5189issn
0975-1009journal_volume
38pub_type
杂志文章abstract：:Reduction of p-benzoquinone to hydroquinol has been studied using Saccharomyces cerevisae and S.uvarum. Maximum conversion of p-Benzoquinone (p-BQ) to Hydroquinol (HQ) at a substrate concentration of 6%. S. cerevisae was found to be better than S. uvarum. No further conversion did take place after 60 hr. Optical innoc...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2007-08-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-02-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2014-02-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：1996-10-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:The Grey Weevil, Myllocerus subfasciatus Guerin, is an important emerging pest of quarantine significance in Solanaceae crops including the eggplant, Solanum melongena. Entomopathogenic nematodes (EPNs) have been shown to be a potential source of safe and effective control of M. subfasciatus. In this study, we determi...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2016-12-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：1989-08-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2001-08-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:Petroleum ether (PE), benzene (BE), chloroform (CE), acetone (AE) and ethanolic (EE) extracts (50-200 or 200 mg/kg, i.p. or 200 mg/kg, p.o.) of dried Abies pindrow leaves, given 30-45 min before showed significant anti-inflammatory (both against acute and sub-acute models), analgesic, barbiturate hypnosis potentiation...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2015-06-01 00:00:00
abstract：:Cisplatin-based chemotherapy has a variety of vascular side effects. The aim of the present study was to evaluate the beneficial effect of melatonin and cisplatin on the alterations in vascular reactivity and structure of cisplatin-treated rats. Phenylephrine (PHE) and KCl-caused concentration-dependent contractions o...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：:An unusual nucleolar organizer region (double NOR) on chromosome 13 was observed in a Down syndrome child [47, XY, +21, dNOR(13)]. The variant chromosome was inherited from the mother [46, XX, dNOR(13)]. The extra chromosome 21 in the proband was maternal origin. The frequency of NOR chromosome association showed rela...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：:Leukotrienes play a part in inflammatory response. The unique role of the enzyme 5-lipoxygenase (5-LOX) in the production of leukotrienes makes it a likely therapeutic target for inflammatory conditions like asthma, rheumatoid arthritis, psoriasis, and inflammatory bowel disease (IBD). The aim of the present study was...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-07-01 00:00:00
