Abstract:
:We tested the hypothesis that in isolated cardiac myocytes, the negative functional effects of cyclic GMP would be blunted when the level of cyclic AMP was increased and that this interaction would be altered in renal hypertensive (One-Kidney-One-Clip, 1K1C) cardiac hypertrophic rabbits. Using isolated control and 1K1C ventricular myocytes, cyclic AMP and cell shortening (%) data were collected: 1) at baseline, 2) after the addition of 8-Br-cGMP 10(-7), -6, -5 M, and 3) after forskolin (10(-6) M), an adenylate cyclase activator, followed by 8-Br-cGMP 10(-7), -6, -5 M. Basal levels of cyclic AMP were similar in control vs. 1K1C myocytes (10.2 +/- 1.6 vs. 11.3 +/- 2.6 pmol/10(5) myocytes). We found that 8-Br-cGMP decreased the percent shortening in a dose related manner in both control myocytes (5.1 +/- 0.6 to 3.2 +/- 0.4%) and hypertrophic myocytes (5.2 +/- 0.4 to 3.6 +/- 0.5). The level of cyclic AMP significantly increased after the addition of 8-Br-cGMP in control myocytes (14.1 +/- 2.1), but not in 1K1C myocytes. Forskolin increased the percent shortening in the control myocytes (3.8 +/- 0.1 to 4.8 +/- 0.4), but no significant increase was noted in the hypertrophic myocytes (3.6 +/- 0.3 to 3.7 +/- 0.3). The level of cyclic AMP significantly increased after the addition of forskolin in both control (13.9 +/- 2.0), and 1K1C cells (14.6 +/- 3.8). Forskolin attenuated the negative functional effects of 8-Br-cGMP in the control (4.8 +/- 0.4 to 3.2 +/- 0.1) and 1K1C myocytes (3.7 +/- 0.3 to 2.7 +/- 0.3). The addition of 8-Br-cGMP did not affect the level of cyclic AMP after forskolin in either control (13.9 +/- 2.0 to 14.8 +/- 2.5) or 1K1C myocytes (14.6 +/- 3.8 to 13.8 +/- 1.9). These data indicated that in hypertrophic cardiac myocytes the negative functional effects of 8-Br-cGMP were similar to control, but the positive functional effects of cyclic AMP were blunted. There was an increase in cyclic AMP levels after addition of 8-Br-cGMP in control but not 1K1C cells. We conclude that in control and hypertrophic myocytes, the effects of cyclic GMP were blunted after forskolin, but this did not seem to be related to cyclic AMP phosphodiesterase activity.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Patel KN,Yan L,Gandhi A,Scholz PM,Weiss HRdoi
10.1007/s003950170075keywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
34-41issue
1eissn
0300-8428issn
1435-1803journal_volume
96pub_type
杂志文章abstract::Total global ischaemia of the normothermic working rat heart caused an initial positive inotropic response characterized by vigorous contractions. After +/- 15 s this response reached a peak whereafter the isotonic contraction amplitude started to decline. After +/- 3.5 min the heart ceased to beat. The low level of h...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01906862
更新日期:1987-05-01 00:00:00
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journal_title:Basic research in cardiology
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journal_title:Basic research in cardiology
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doi:10.1007/BF01908341
更新日期:1981-07-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
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更新日期:2003-07-01 00:00:00
abstract::We describe diastolic properties of the myocardium in terms of stress-strain relations. In a mathematical analysis, the equation sigma = alpha(e beta.epsilon-1) of the stress-strain curve can be changed by an increase in the exponent beta or the multiplicative constant alpha. It can be experimentally shown that in hyp...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/978-3-662-11289-2_26
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journal_title:Basic research in cardiology
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doi:10.1007/s00395-018-0707-9
更新日期:2018-10-29 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:
更新日期:1985-01-01 00:00:00
abstract::The effects of 3 hours of ischemia and 1 hour of reperfusion on biochemical, physiological and ultrastructural parameters were studied in 12 dogs. In the ischemic subendocardium without reperfusion, mitochondrial losses of adenine (ATP + ADP + AMP) and pyridine (NAD + NADH) nucleotides far exceeded those observed in w...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01908307
更新日期:1984-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-03-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01910780
更新日期:1976-07-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00788668
更新日期:1992-11-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF02190542
更新日期:1991-03-01 00:00:00
abstract::The focal adhesion and microfilament-associated protein VASP is a major substrate of both cAMP- and cGMP-dependent protein kinase in intact human platelets. The recent elucidation of the primary VASP structure and identity of VASP binding proteins suggest that VASP is an important component of focal contacts which lin...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00788712
更新日期:1996-09-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907225
更新日期:1987-11-01 00:00:00
abstract::The quantitative predominance of free and total cholesterol over the amount of mural calcium is a most significant criterion of healthy human coronary arteries during the whole life span (0-90 years). However, this normal ratio increasingly changes as soon as arteriosclerotic alterations of the coronary walls set in. ...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/978-3-642-85660-0_15
更新日期:1994-01-01 00:00:00
abstract::To determine the effect of magnesium on myocardial function and oxidative metabolism after reperfusion, isolated rat hearts perfused retrogradely with erythrocyte-enriched medium (0.4 mM palmitate bound to 0.4 mM albumin, 11 mM glucose) were subjected to 60 minutes of no-flow ischemia followed by 60 minutes of reperfu...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00795204
更新日期:1994-07-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907975
更新日期:1989-05-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/BF02650357
更新日期:1989-01-01 00:00:00
abstract::Disregarding the influence of thickness on elevated strength of isolated preparations inevitably leads to erroneous tension-frequency relations, especially in the range of high frequencies. Thus, much of the confusion in interpreting the atypical negative staircase phenomenon of the rat heart is due to this. In view o...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/978-3-662-11289-2_25
更新日期:1987-01-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00788500
更新日期:1995-09-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/978-3-662-11038-6_28
更新日期:1990-01-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s003950170042
更新日期:2001-07-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/978-3-642-72376-6_2
更新日期:1984-01-01 00:00:00
abstract::From January 2020, coronavirus disease (COVID-19) originated in China has spread around the world. The disease is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The presence of myocarditis, cardiac arrest, and acute heart failure in COVID-19 patients suggests the existence of a relationshi...
journal_title:Basic research in cardiology
pub_type: 社论
doi:10.1007/s00395-020-0791-5
更新日期:2020-04-09 00:00:00
abstract::The influence of decapitation, ketamine and thiopental anesthesia on some properties of rat heart mitochondria was compared. Polarographic analysis were performed, oxygen consumption rates, respiratory control index, ADP : O ratio and oxidation of exogenous NADH were determined. Electronmicroscopic and gasometric exam...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907952
更新日期:1981-03-01 00:00:00
abstract::We have used x-ray microangiography to investigate the influence of EDRF and endothelin-1 on arterial diameters (70-800 microns) at bifurcations in the isolated rabbit ear and the "optimality" of its branching geometry. The median value of the junction exponent x (which is given by d0x = d1x + d2x, where d0, d1 and d2...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/978-3-642-72461-9_10
更新日期:1991-01-01 00:00:00
abstract::During myocardial ischemia, both the myocardial and serum TNFalpha concentrations are rapidly increased within the area at risk. With prolongation of ischemia and development of cardiomyocyte necrosis, the TNFalpha concentration increases also in the surrounding viable portions of the myocardium. Indeed, in the scenar...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s00395-003-0431-x
更新日期:2004-01-01 00:00:00
abstract::We chronically implanted hygroscopic occluders around the left circumflex coronary artery in 49 anesthetized young male domestic pigs and we studied the development of a collateral circulation at 4, 8, 12, and 26 weeks after implantation. At these time intervals groups of animals were again anesthetized, the hearts we...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01908204
更新日期:1989-09-01 00:00:00
abstract::In dog myocardium, the changes in the levels of creatine phosphate, inorganic phosphate, ATP, ADP, AMP, adenosine and inosine with 8 min of ischemia and subsequent reperfusion for 2, 4, 8, 16 and 32 min have been followed. Creatine phosphate and inorganic phosphate recovered completely within a few minutes as did the ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907461
更新日期:1986-07-01 00:00:00
abstract::The mitochondrial electrochemical gradient (Δp), which comprises the pH gradient (ΔpH) and the membrane potential (ΔΨ), is crucial in controlling energy transduction. During myocardial ischemia and reperfusion (IR), mitochondrial dysfunction mediates superoxide (·O2-) and H2O2 overproduction leading to oxidative injur...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-017-0626-1
更新日期:2017-07-01 00:00:00