Inhibitory activity of antibodies against the human atrial 5-HT(4)receptor.

Abstract:

:Antibodies directed against the second extracellular loop of G protein-coupled receptors have been shown to exert "agonist-like" activities. In order to test the hypothesis that this is a general phenomenon, antibodies were raised in rabbits against a synthetic peptide corresponding to the second extracellular loop of the newly sequenced human cardiac 5-HT(4)receptor. The antibodies were affinity-purified and shown to recognize the 5-HT(4)receptor in immunoblots of Chinese hamster ovary (CHO) cells expressing the receptor. The antibodies had no intrinsic effect but could depress the activation of L -type calcium channel induced by serotonin in human atrial cells. This antagonist-like effect was exerted both by intact IgG and by Fab fragments. These results are physiologically important since it has been shown that the 5-HT(4)receptor could be a target for autoantibodies in mothers at risk of giving birth to children with neonatal atrio-ventricular block.

journal_name

J Mol Cell Cardiol

authors

Sallé L,Eftekhari P,Aupart M,Cosnay P,Hoebeke J,Argibay JA

doi

10.1006/jmcc.2000.1312

keywords:

subject

Has Abstract

pub_date

2001-03-01 00:00:00

pages

405-17

issue

3

eissn

0022-2828

issn

1095-8584

pii

S0022-2828(00)91312-9

journal_volume

33

pub_type

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