Abstract:
:Post-transcriptional gene silencing (PTGS) has been successfully used to modify seed lipids in oilseed crops like soybean, canola and sunflower. Conventionally, PTGS has been induced by transforming the plants with either antisense or co-suppression constructs targeted against key seed lipid biosynthesis genes. A major drawback of this approach has been the recovery of only a modest proportion of silenced individuals from large populations of transgenic plants. In this report we show that inverted-repeat DNA constructs containing an intron encoding RNA with a hairpin structure can induce PTGS with very high frequency.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Singh S,Green A,Stoutjesdijk P,Liu Qkeywords:
subject
Has Abstractpub_date
2000-12-01 00:00:00pages
925-7issue
6eissn
0300-5127issn
1470-8752journal_volume
28pub_type
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