Abstract:
:We report a novel, highly sensitive and selective method for the detection and quantification of acyl-CoA esters from Arabidopsis thaliana. Extracted acyl-CoA esters were derived to their fluorescent acyl-etheno-CoA esters, separated by ion-paired reversed-phase HPLC, and detected fluorometrically. We report the composition and concentration of the acyl-CoA pool in mature A. thaliana leaves, and during storage-lipid breakdown in 2-day-old seedlings. The concentrations measured were in the 1-4 microM range for both tissue types. To our knowledge, this is the first time that reliable estimates of acyl-CoA ester concentrations have been made for plants.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Larson TR,Graham IAkeywords:
subject
Has Abstractpub_date
2000-12-01 00:00:00pages
575-7issue
6eissn
0300-5127issn
1470-8752journal_volume
28pub_type
杂志文章abstract::Bloom's syndrome (BS) is a rare autosomal recessive disorder characterized by genomic instability and cancer predisposition. The underlying genetic defect is mutation of the BLM gene, producing deficiency in the RecQ helicase BLM (Bloom's syndrome protein). The present article begins by introducing BLM and its binding...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0370553
更新日期:2009-06-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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更新日期:2013-02-01 00:00:00
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journal_title:Biochemical Society transactions
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0371270
更新日期:2009-12-01 00:00:00
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journal_title:Biochemical Society transactions
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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journal_title:Biochemical Society transactions
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更新日期:2010-08-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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更新日期:2010-02-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20140155
更新日期:2014-10-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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更新日期:2013-04-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0310528
更新日期:2003-06-01 00:00:00
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journal_title:Biochemical Society transactions
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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更新日期:2019-02-28 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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更新日期:2017-10-15 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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更新日期:2013-08-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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更新日期:2016-04-15 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0360033
更新日期:2008-02-01 00:00:00
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journal_title:Biochemical Society transactions
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更新日期:2011-10-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章
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更新日期:2013-08-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0361482
更新日期:2008-12-01 00:00:00