Abstract:
BACKGROUND & AIMS:p16(INK4a) is a cell cycle inhibitor and a major tumor-suppressor protein, but the regulation of p16(INK4a) is poorly understood and the physiologic settings in which it exerts its antiproliferative effects are unknown. A role for p16(INK4a) in intestinal neoplasia is suggested by the observation that the promoter region is methylated in a subset of human colon tumors. We examined the expression of the protein in specimens representing the full spectrum of neoplastic progression in the human colon and determined whether expressing cells showed evidence of cell cycle inhibition. METHODS:We studied p16(INK4a) expression by immunoprecipitation, immunoblotting, reverse-transcription polymerase chain reaction (RT-PCR), immunohistochemistry, and immunofluorescence in matched normal and neoplastic colonic tissue from 70 patients. RESULTS:p16(INK4a) expression was very low in normal mucosa, with staining observed in rare epithelial cells at the base of crypts. A distinctly higher expression was found in 4 of 7 aberrant crypt foci, 32 of 36 adenomas, 18 of 28 primary carcinomas, and 5 of 5 metastatic carcinomas. Within each neoplasm p16(INK4a) staining was heterogeneous, with higher expression commonly seen in areas bordering normal tissue. p16(INK4a) staining correlated inversely with that of Ki67, cyclin A, and the retinoblastoma protein, suggesting that cell cycle progression was inhibited. CONCLUSIONS:These results suggest that p16(INK4a) expression begins in the earliest detectable stages of neoplastic progression in the human colon and exerts a continuous, piecemeal constraint on tumor growth.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Dai CY,Furth EE,Mick R,Koh J,Takayama T,Niitsu Y,Enders GHdoi
10.1053/gast.2000.17952keywords:
subject
Has Abstractpub_date
2000-10-01 00:00:00pages
929-42issue
4eissn
0016-5085issn
1528-0012pii
S0016508500239978journal_volume
119pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract:BACKGROUND & AIMS:Triage of patients with acute upper gastrointestinal hemorrhage (UGIH) has traditionally required urgent upper endoscopy. The aim of this study is to evaluate the use of artificial neural network for nonendoscopic triage. METHODS:A cohort of 387 patients was used to train (n = 194) and internally val...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.10.037
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND & AIMS:Cyclosporin A specifically suppresses hepatitis C virus (HCV) replication in vitro at clinically achievable concentrations. In this study, we investigated the mechanisms of action of cyclosporin A against HCV replication. METHODS:The in vitro effects of cyclosporin A on HCV replication were analyzed ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2005.06.031
更新日期:2005-09-01 00:00:00
abstract::Weight gain and obesity are major risk factors for conditions and diseases ranging from insulin resistance and type 2 diabetes mellitus to atherosclerosis and the sequelae of nonalcoholic fatty liver disease. A chronic, subacute state of inflammation often accompanies the accumulation of excess lipid in adipose tissue...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2007.03.059
更新日期:2007-05-01 00:00:00
abstract:BACKGROUND & AIMS:Studies on the early steps in the life cycle of hepatitis B virus have been hampered by the lack of readily available target cells. In this study, we mapped a defined virus attachment site to primary hepatocytes that is essential for infection. METHODS:We used purified virus particles from human carr...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2005.03.090
更新日期:2005-07-01 00:00:00
abstract:BACKGROUND/AIMS:Despite putative roles of calcium in biliary physiology and gallstone formation, quantitative aspects of calcium binding to bile salt (BS) monomers, simple micelles, mixed micelles, and vesicles, which constitute the lipid aggregates in bile, remain unexplored. METHODS:Calcium activity was measured usi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(94)90134-1
更新日期:1994-09-01 00:00:00
abstract::The purpose of this study is to determine whether lower esophageal sphincter (LES) incompetency is a common occurrence in patients with liver cirrhosis and contributes to the development of variceal bleeding. Resting LES pressure (17.8 +/- 1.1 mm Hg) in 35 patients with cirrhosis was similar to that of our control pop...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract:BACKGROUND & AIMS:Transferrin receptor 2 (TfR2) plays a key role in the regulation of iron metabolism. Mutations of TfR2 in humans cause type 3 hereditary hemochromatosis. Although highly expressed in liver, several studies have reported TfR2 expression in other tissues. To determine the contribution of liver expressed...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2006.11.028
更新日期:2007-01-01 00:00:00
abstract::Serum gastrin and gastric endocrine cell numerical densities were examined in 22 patients with long-standing Zollinger-Ellison syndrome who were receiving either ranitidine, omeprazole, or other antisecretory drugs (SMS 201-995 or pirenzepine with or without ranitidine) for long periods of time. Fifteen patients had i...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(89)91620-x
更新日期:1989-04-01 00:00:00
abstract::Sepsis following the placement of a transjugular intrahepatic portosystemic shunt (TIPS) has been reported in up to 10% of cases. This report describes an unusual case of polymicrobial gram-negative sepsis after placement of TIPS associated with a cholangiographically documented fistulous connection between the biliar...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1996.v111.pm8898650
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND & AIMS:Immune-nonimmune cell interactions modulate mucosal immunity. We investigated the expression of adhesion molecules by intestinal fibroblasts, the effect of immune cell-derived factor on fibroblast binding of T cells, and the consequences of interfering with adhesion molecule expression on fibroblast-T...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(99)70447-6
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND & AIMS:Ethanol causes both tolerance and sensitization of Kupffer cells. This study was designed to evaluate temporal effects of ethanol in an attempt to understand this paradox. METHODS:Rats were given ethanol (4 g/kg body wt) intragastrically, and Kupffer cells were isolated 0-48 hours later. After additi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(98)70211-2
更新日期:1998-08-01 00:00:00
abstract::Congenital esophageal stenosis (CES) is a rare disorder with narrowed esophageal lumen that presents as dysphagia from childhood and that is often associated with tracheobronchial remnants or webs. The pathogenesis of CES is unknown. The aim of this study was to examine the histological and immunohistochemical feature...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(95)90294-5
更新日期:1995-07-01 00:00:00
abstract::Ornithine decarboxylase activity is known to be increased in certain premalignant conditions. We determined the activity of this enzyme in mucosal biopsy specimens from 15 patients with Barrett's esophagus. Ornithine decarboxylase was greater in Barrett's mucosa than in squamous esophageal or gastric mucosa. In Barret...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(88)90259-4
更新日期:1988-03-01 00:00:00
abstract:BACKGROUND & AIMS:Patients with chronic hepatitis B virus (HBV) infection have a high risk for developing hepatocellular carcinoma (HCC). Patients with lower levels of hepatitis B surface antigen (HBsAg) have higher chances of losing HBsAg than those with high levels. However, little is known about whether higher level...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2012.02.007
更新日期:2012-05-01 00:00:00
abstract::Adenomatous polyps are the most common neoplastic findings discovered in people who undergo colorectal screening or who have a diagnostic work-up for symptoms. It was common practice in the 1970s for these patients to have annual follow-up surveillance examinations to detect additional new adenomas and missed synchron...
journal_title:Gastroenterology
pub_type: 杂志文章,实务指引,评审
doi:10.1053/j.gastro.2006.03.012
更新日期:2006-05-01 00:00:00
abstract:BACKGROUND & AIMS:Ethanol impairs the mitochondrial transport of reduced glutathione (GSH), resulting in lower mitochondrial GSH (mGSH) levels. Our purpose was to evaluate the role of acetaldehyde on the regulation of mGSH in HepG2 cells. METHODS:mGSH levels and transport, mitochondrial membrane microviscosity, and li...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2003.50089
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND & AIMS:Practice guidelines recommend that endoscopists spend at least 7 minutes examining the colonic mucosa during colonoscopy withdrawal to optimize polyp yield. The aim of this study was to determine if the implementation of an institution-wide policy of colonoscopy withdrawal time > or = 7 minutes was as...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2008.08.024
更新日期:2008-12-01 00:00:00
abstract:BACKGROUND & AIMS:Notch downstream targets are fundamental to intestinal cell lineage commitment and are suggested as therapeutic targets for colon cancer cells. However, the role of endogenous Notch signaling through receptor-ligand interaction, and effects of its longer term down-regulation on intestinal homeostasis,...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2008.05.050
更新日期:2008-09-01 00:00:00
abstract::Reduction in caloric intake was associated with a greater absolute rise in the serum bilirubin concentration in patients with Gilbert's syndrome and partial hepatic bilirubin uridine diphosphate glucuronyltransferase (UDPG-T) dysfunction compared to patients with hemolytic unconjugated hyperbilirubinemia and normal su...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1975-07-01 00:00:00
abstract:BACKGROUND/AIMS:In vivo hepatic 31P magnetic resonance spectroscopy (MRS) can provide information on hepatic energy metabolism, phospholipid substrates, and hepatocyte lipid bilayers. The aim of this study was to ascertain the effects of alcohol ingestion on hepatic 31P spectral variables. METHODS:Twenty-six chronic a...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(95)90451-4
更新日期:1995-03-01 00:00:00
abstract:BACKGROUND & AIMS:There have been conflicting results on a cell of origin in pancreatic regeneration. These discrepancies predominantly stem from lack of specific markers for the pancreatic precursors/stem cells, as well as differences in the targeted cells and severity of tissue injury in the experimental models so fa...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2011.07.003
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND & AIMS:Probiotics can reduce symptoms of irritable bowel syndrome (IBS), but little is known about their effects on psychiatric comorbidities. We performed a prospective study to evaluate the effects of Bifidobacterium longum NCC3001 (BL) on anxiety and depression in patients with IBS. METHODS:We performed ...
journal_title:Gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.1053/j.gastro.2017.05.003
更新日期:2017-08-01 00:00:00
abstract:BACKGROUND & AIMS:Patients affected by familial adenomatous polyposis (FAP) are at increased risk of developing duodenal adenomas. In this study, cell proliferation of duodenal mucosa was analyzed to detect kinetic abnormalities related to cancer risk. METHODS:Duodenal biopsy specimens were collected in three groups o...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1997.v113.pm9322510
更新日期:1997-10-01 00:00:00
abstract::First, and most importantly, the standard of care for treating adenomatous polyps is polypectomy and not therapy with NSAIDs. The initial clinical observation by Waddell and Loughry in 1983 that sulindac treatment influenced rectal polyps in patients with FAP has led to a considerable amount of research, commentary, a...
journal_title:Gastroenterology
pub_type: 评论,社论
doi:10.1016/0016-5085(95)90235-x
更新日期:1995-04-01 00:00:00
abstract::This study is an examination of the association between Crohn's disease and psychiatric illness using careful gastrointestinal evaluation, a structured psychiatric interview of known reliability and validity, and explicit diagnostic criteria for psychiatric illness. A proband sample of 50 subjects with Crohn's disease...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1984-02-01 00:00:00
abstract::There are apparent similarities in the mechanisms of the intestinal phase of exocrine and pancreatic polypeptide (PP) secretion by the pancreas. To characterize this relationship, we measured incremental responses of protein, bicarbonate, and PP to graded doses of intravenous secretin, caerulein, CCK8, CCK33, bethanec...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1984-09-01 00:00:00
abstract::Somatostatin-14 and somatostatin-28 are biologically active peptides derived from the posttranslational cleavage of prosomatostatin. Because both peptides are found in variable concentrations in the gastrointestinal (GI) tract and pancreas, it has been contended that somatostatin-28 is either an intermediate in the pr...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(90)91151-u
更新日期:1990-11-01 00:00:00
abstract::From 1979 to 1984, we followed the cases of 3 men (aged 13, 31, and 75 yr) and 2 women (aged 38 and 45 yr who had never used oral contraceptives) suffering from liver adenomatosis, an uncommon lesion consisting of numerous benign adenomas in an otherwise normal hepatic parenchyma. During the same period, we observed 2...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
abstract:BACKGROUND & AIMS:The anti-cancer effects of vegetables and fruit have been investigated extensively, but the association between vegetable and fruit consumption and risk of hepatocellular carcinoma (HCC) has not been quantified. We performed a meta-analysis of observational studies to clarify the association. METHODS...
journal_title:Gastroenterology
pub_type: 杂志文章,meta分析,评审
doi:10.1053/j.gastro.2014.08.005
更新日期:2014-11-01 00:00:00
abstract:BACKGROUND & AIMS:Toll-like receptors (TLRs) represent a class of transmembrane pattern recognition receptors essential for microbial recognition and control of innate immune responses. Commensal bacteria play an important role in maintaining tolerance and active stability of the intestinal epithelial barrier by suppre...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.02.056
更新日期:2007-04-01 00:00:00