Abstract：
:We describe the modification of reactive actin sulfhydryls by S-nitrosoglutathione. Kinetics of S-nitrosylation and denitrosylation suggest that only one cysteine of actin is involved in the reactions. By using the bifunctional sulfhydryl cross-linking reagent N,N'-1,4-phenylenebismaleimide and the monofunctional reagent N-iodoacetyl-N'-(5-sulpho-1-naphthyl)ethylenediamine, we identified this residue as Cys374. The time course of filament formation followed by high-shear viscosity changes revealed that S-nitrosylated G-actin polymerizes less efficiently than native monomers. The observed decrease in specific viscosity at steady state is due mainly to a marked inhibition of filament end-to-end annealing and, partially, to a reduction in F-actin concentration. Finally, S-nitrosylated actin acts as nitric oxide donor showing a fast, potent vasodilating activity at unusually low concentrations, being comparable with that of low molecular weight nitrosothiols.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Dalle-Donne I,Milzani A,Giustarini D,Di Simplicio P,Colombo R,Rossi Rdoi
10.1023/a:1005671319604keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
171-81issue
2eissn
0142-4319issn
1573-2657journal_volume
21pub_type
杂志文章abstract：:The effects of 2,3-butanedione monoxime (BDM) on contraction characteristics were studied at 5 degrees C in single intact fibres isolated from the tibialis anterior muscle of the frog. The force-velocity relation was determined using the controlled-velocity method in either whole fibres or short fibre segments in whic...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01737994
更新日期：1992-10-01 00:00:00
abstract：:Guinea-pig soleus muscles were denervated and electrically stimulated for periods of 43 to 66 days. Stimuli were in 1 s bursts of 40 Hz pulses, repeated every 5 min. Other guinea-pigs were denervated for 82 days without stimulation and, in a third group, the soleus muscle was necrotized and allowed to regenerate witho...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1018619503456
更新日期：1997-10-01 00:00:00
abstract：:The ability for muscle to repeatedly generate force is limited by fatigue. The cellular mechanisms behind muscle fatigue are complex and potentially include breakdown at many points along the excitation-contraction pathway. In this paper we construct a mathematical model of the skeletal muscle excitation-contraction p...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-007-9125-6
更新日期：2007-01-01 00:00:00
abstract：:Tropomyosins (Tm) in humans are expressed from four distinct genes and by alternate splicing >40 different Tm polypeptide chains can be made. The functional Tm unit is a dimer of two parallel polypeptide chains and these can be assembled from identical (homodimer) or different (heterodimer) polypeptide chains provided...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-013-9353-x
更新日期：2013-08-01 00:00:00
abstract：:Although a substantial literature exists on the in vitro polymerization of purified myosin, little is known about native thick filament assembly, remodeling or turnover. We have recently described a cell-free system (Bouche et al., 1988) to examine the interactions between thick filaments and soluble, newly synthesize...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01774035
更新日期：1991-04-01 00:00:00
abstract：:The loss of muscle force and power during fatigue from intense contractile activity is associated with, and likely caused by, elevated levels of phosphate ([Formula: see text]) and hydrogen ions (decreased pH). To understand how these deficits in muscle performance occur at the molecular level, we used direct measurem...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-018-9499-7
更新日期：2018-08-01 00:00:00
abstract：:Our current understanding of the structure of the thin filaments of muscle and the molecular mechanism by which thin filaments regulate muscle contraction are reviewed and discussed. We focus, in particular, on the crucial role played by Jean Hanson in these studies and on later contributions from those whose work she...
journal_title：Journal of muscle research and cell motility
pub_type： 传,历史文章,杂志文章,评审
doi：10.1007/s10974-004-3148-z
更新日期：2004-01-01 00:00:00
abstract：:Myomesin-I (also known as Skelemin) is a approximately 185 kDa protein, which is highly expressed in striated muscle. It contains the prototypic class-I (type-III fibronectin) and class-II (C2-immunoglobulin) motifs. Previous studies have shown the presence of Myomesin-I at the M-line of the sarcomere, where it is tho...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-008-9137-x
更新日期：2008-01-01 00:00:00
abstract：:Myosin has been purified from chicken pectoralis muscle at various stages of development, from 10 days' incubation to approximately 10 months after hatching. Embryonic myosin from the earliest stage showed a high level of ATPase activity, similar to that obtained for adult pectoralis myosin. Two-dimensional peptide ma...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00712161
更新日期：1983-12-01 00:00:00
abstract：:The cellular function of the giant protein titin in striated muscle is a major focus of scientific attention. Particularly, its role in passive mechanics has been extensively investigated. In strong contrast, the structural details of this filament are very poorly understood. To date, only a handful of atomic models f...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-005-9017-6
更新日期：2005-01-01 00:00:00
abstract：:The regulated assembly of actin filaments is essential in nearly all cell types. Studying actin assembly dynamics can pose many technical challenges. A number of these challenges can be overcome by using microfluidics to observe and manipulate single actin filaments under an optical microscope. In particular, microflu...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-019-09564-4
更新日期：2020-03-01 00:00:00
abstract：:The L-type Ca2+ channel in skeletal muscle (alpha1S) is essential for excitation-contraction (EC) coupling. Previous studies using chimeras composed of alpha1S together with alpha1C or alpha1M demonstrated the importance of the alpha1S II-III loop and of a smaller subdomain (residues 720-764; 'ECC') in skeletal EC cou...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1024830132118
更新日期：2003-01-01 00:00:00
abstract：:Previous studies have shown that lengthening contractions, in contrast to isometric contractions, readily result in sustained malfunctioning of the exercised muscles. The present study was performed to investigate whether an exercise period with many (240) lengthening contractions (LC) results in alterations in muscle...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005345603882
更新日期：1998-05-01 00:00:00
abstract：:The optimal utilization of energy substrates in muscle fibers is of primary importance for muscle contraction and whole body physiology. This study aimed to investigate the age-related changes in some indicators of glucose catabolism and fatty acid oxidation in muscles of growing rabbits. Longissimus lumborum (fast-tw...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-004-2768-7
更新日期：2004-01-01 00:00:00
abstract：:Skeletal muscle fibers exhibit plasticity of their physiological and biochemical properties in response to the firing pattern from the innervating motor neuron. In particular, the gene expression pattern generally characteristic of a slow twitch fiber can be induced in a fast twitch fiber by chronic slow fiber type el...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-006-9080-7
更新日期：2006-01-01 00:00:00
abstract：:Extensor digitorum longus muscles of normal mice (C57BL/10ScSn hereafter called C57) were orthotopically transplanted into dystrophin-deficient mice (mdx) and reciprocally, mdx Extensor digitorum longus muscles were transplanted into C57 mice. After an initial phase of degeneration, transplanted muscles regenerate nea...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00140322
更新日期：1996-02-01 00:00:00
abstract：:Five monoclonal antibodies that react with different regions of myosin light chain 1 from human ventricular myocardial muscle were used to obtain information on interactions between the light chain 1 and heavy chains and generally on the tertiary structure of the light chain 1 within the myosin head. We performed West...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01766461
更新日期：1992-06-01 00:00:00
abstract：:Myosin heavy chain species were investigated during development in avian pectoralis major muscles (type IIB fibres) by high resolution anion-exchange chromatography of the myosin head region, subfragment-1. At 15 days in ovo four distinct fast-type heavy chain species, I, II, III and IV, in order of elution, were iden...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01745118
更新日期：1991-06-01 00:00:00
abstract：:It has been proposed that in striated muscle inositol 1,4,5-trisphosphate (InsP3) may serve as a chemical transmitter linking membrane depolarization to Ca(2+)-release from the sarcoplasmic reticulum. Key to that hypothesis of excitation-concentration (EC) coupling was the observation that skinned muscle fibres contra...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01738039
更新日期：1992-08-01 00:00:00
abstract：:Telokin, a 17 kDa smooth muscle specific protein, consists of the C-terminal domain of MLCK, is phosphorylated by PKA and PKG at Ser13 in vivo (Wu et al. (1998) J Biol Chem 273: 11362-11369; Walker et al. (2001) J. Biol Chem 276: 24519-24524) and is proposed to induce Ca2+-desensitization through activation of myosin ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-004-7807-x
更新日期：2004-01-01 00:00:00
abstract：:Invertebrate mini-titins are members of a class of myosin-binding proteins belonging to the immunoglobulin superfamily that may have structural and/or regulatory properties. We have isolated mini-titins from three molluscan sources: the striated and smooth adductor muscles of the scallop, and the smooth catch muscles ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00141557
更新日期：1993-12-01 00:00:00
abstract：:A set of five differential equations has been found which gives a satisfactory account of the isotonic and isometric properties of striated muscle. Four of these differential equations give an equally satisfactory account of the results of length-drive experiments with sinusoidal variation of length. In this case, the...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/BF00713256
更新日期：1984-10-01 00:00:00
abstract：:To study the development of muscle-specific features during myogenesis, we analysed the ultrastructure and voltage-dependent currents of frog embryonic skeletal myocytes maintained in culture for 10 days. The cells were maintained under culture conditions that prevented cell division, fusion and cell contacts with neu...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005506216009
更新日期：1999-08-01 00:00:00
abstract：:Because complex structural differences in adult extraocular muscles may have physiological and pathophysiological significance, the three-dimensional pattern of myosin heavy chain (MHC) isoform expression within the orbital and global layers of the muscle bellies compared with the distal tendon ends was quantitatively...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005656312518
更新日期：1999-11-01 00:00:00
abstract：:Homozygous calsequestrin 2 (CASQ2) point mutations leads to catecholaminergic polymorphic ventricular tachycardia: a common pathogenetic feature appears to be the drastic reduction of mutant CASQ2 in spite of normal transcription. Comparative biochemical analysis of R33Q and D307H knock in mutant mice identifies diffe...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-020-09587-2
更新日期：2020-09-01 00:00:00
abstract：:It is generally thought that the rapid relaxation of fast muscles is facilitated by the Ca(2+) binding protein parvalbumin (Parv). Indeed superfast swimbladder (SWB) muscle of toadfish contains the largest concentration of this protein ever observed (up to 1.5 mM). At 15 degrees C toadfish perform a 100 Hz call, 400 m...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-009-9175-z
更新日期：2009-01-01 00:00:00
abstract：:The time course of shortening at zero load was studied by the slack test method during tetanic contractions in isolated, single, slow muscle fibres of the crayfish. In 28 of 32 shortenings (from 14 different fibres) a biphasic shortening was seen, which consisted of an initial high-velocity phase lasting 3.3-20.8 ms a...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01738251
更新日期：1992-12-01 00:00:00
abstract：:We investigated the expression of myosin subunits (myosin heavy chains) as well as light chains and the in vivo phosphorylation of the phosphorylatable myosin light chain in the heart ventricle of the adult male European hamster (Cricetus cricetus L.). Two myosin heavy chain isoenzymes could be detected under native a...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01738429
更新日期：1992-02-01 00:00:00
abstract：:There is evidence to suggest that 8 nm calcium transport particles in the sarcoplasmic reticulum are involved in the regulation of twitch properties in adult muscles. We have studied ultrastructural characteristics of the sarcoplasmic reticulum in relation to previously defined physiological changes that take place in...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01753413
更新日期：1986-04-01 00:00:00
abstract：:Ryanodine receptors (RyRs) are huge homotetrameric Ca2+ release channels localized to the sarcoplasmic reticulum. RyRs are responsible for the release of Ca2+ from the SR during excitation-contraction coupling in striated muscle cells. Recent revolutionary advancements in cryo-electron microscopy have provided a numbe...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-020-09575-6
更新日期：2020-02-10 00:00:00
