Abstract:
:The simultaneous administration of the DNA topoisomerase II inhibitor etoposide (0.15 mM) and 1,25-dihydroxyvitamin D3 (VD3) (10 nM) synergistically induced the differentiation of HL-60 human promyelocytic leukemia cells. Similar results were obtained using U-937 human promonocytic cells, or the topoisomerase II inhibitors doxorubicin (15 nM) and mitoxantrone (2.5 nM). When sequential treatments were used, pre-incubation with VD3 had little effect on the subsequent action of etoposide, while pre-incubation with etoposide greatly potentiated the subsequent action of VD3. In addition, etoposide treatment stimulated VD3 binding activity and increased VD3 receptor mRNA and protein levels. The increase in hormone receptor expression may explain, at least in part, the capacity of topoisomerase inhibitors to potentiate the differentiation inducing activity of VD3.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Torres R,Calle C,Aller P,Mata Fdoi
10.1023/a:1007089632152keywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
157-62issue
1-2eissn
0300-8177issn
1573-4919journal_volume
208pub_type
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