Inhibition of MicroRNA miR-101-3p on prostate cancer progression by regulating Cullin 4B (CUL4B) and PI3K/AKT/mTOR signaling pathways.

Abstract:

:To probe into the efffects of miR-101-3p via regulating CUL4B within PI3K/AKT/mTOR signaling pathway on progression of prostate cancer (PCA). Western blot and qRT-PCR were adopted to detect CUL4B and miR-101-3p expressions in 75 cases with PCA . The cellular strains of PCA (LNCaP and PC3) were chose as the objects to check the targeting correlation between CUL4B and miR-101-3p through dual-luciferase reporter experiments. LNCaP cells and PC3 cells were randomly divided into the blank group, miR-101-3p mimic group, siRNA negative control (NC) group, CUL4B siRNA group and CUL4B siRNA plus the miR-101-3p inhibitor group. Cellular bioactivity measurement was done via Cell-Light EDU, MTT, Annexin-V-FITC/PI, scratch-heal experiments and invasion tests of Transwell. MiR-101-3p expression was decreased more signally in tumor tissues than in normal tissues adjacent to the cancer. MiR-101-3p inhibited cellular proliferating, migrating and invasion. Nevertheless, it promoted cellular apoptosis, up-regulated apoptotic proteins as well as down-regulated anti-apoptotic proteins. CUL4B siRNA and miR-101-3p simulation were similar in terms of their outcomes. Nonetheless, these results could be reversed through the miR-101-3p inhibitor. Besides, CUL4B siRNA and the simulation halted a serious of PI3K signal in PCA cells. MiR-101-3p expression was down-regulated in PCA patients. CUL4B was upregulated in PCA patients. Moreover, miR-101-3p suppressed cellular invasion, migration, proliferation and led to cellular apoptosis, which might be related to the PI3K/AKT/mTOR signaling pathway suppression. Finally, we found, MiR-101-3P suppressed PCA progression via aiming for CUL4B, which may offer the new molecular target for PCA clinical treatment.

journal_name

Bioengineered

journal_title

Bioengineered

authors

Gu Z,You Z,Yang Y,Ding R,Wang M,Pu J,Chen J

doi

10.1080/21655979.2021.1949513

keywords:

["CUL4B","PI3K\/AKT\/mTOR","miR-101-3p","prostate cancer"]

subject

Has Abstract

pub_date

2021-12-01 00:00:00

pages

4719-4735

issue

1

eissn

2165-5979

issn

2165-5987

journal_volume

12

pub_type

杂志文章

相关文献

文献大全
  • Toward a bioengineered heparin: challenges and strategies for metabolic engineering of mammalian cells.

    abstract::Heparin is the most widely used pharmaceutical to control blood coagulation in modern medicine. A health crisis that took place in 2008 led to a demand for production of heparin from non-animal sources. Since Chinese hamster ovary (CHO) cells are capable of producing heparan sulfate (HS), a related polysaccharide natu...

    journal_title:Bioengineered

    pub_type: 评论,杂志文章

    doi:10.4161/bioe.20902

    authors: Baik JY,Wang CL,Yang B,Linhardt RJ,Sharfstein S

    更新日期:2012-07-01 00:00:00

  • Engineering of N-acetylglucosamine metabolism for improved antibiotic production in Streptomyces coelicolor A3(2) and an unsuspected role of NagA in glucosamine metabolism.

    abstract::N-acetylglucosamine (GlcNAc), the monomer of chitin and constituent of bacterial peptidoglycan, is a preferred carbon and nitrogen source for streptomycetes. Recent studies have revealed new functions of GlcNAc in nutrient signaling of bacteria. Exposure to GlcNAc activates development and antibiotic production of Str...

    journal_title:Bioengineered

    pub_type: 评论,杂志文章

    doi:10.4161/bioe.21371

    authors: Świątek MA,Urem M,Tenconi E,Rigali S,van Wezel GP

    更新日期:2012-09-01 00:00:00

  • A simplified purification method for AAV variant by polyethylene glycol aqueous two-phase partitioning.

    abstract::Adeno-Associated Virus (AAV) has been widely used for in vivo study and preclinical therapy due to its ability to mediate long-term transgene expression, its lack of pathogenicity and low immunogenicity. It has been found that AAV has more than ten serotypes, which each transfect certain types of cells in the viral in...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.22293

    authors: Guo P,Xiao X,El-Gohary Y,Paredes J,Prasadan K,Shiota C,Wiersch J,Welsh C,Gittes GK

    更新日期:2013-03-01 00:00:00

  • MultiBac turns sweet.

    abstract::The baculovirus/insect cell system has proven to be a powerful tool for the expression of eukaryotic proteins. Therapeutics, especially in the field of vaccinology, are often composed of several different protein subunits. Conventional baculoviral expression schemes largely lack efficient strategies for simultaneous m...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.4161/bioe.22327

    authors: Palmberger D,Klausberger M,Berger I,Grabherr R

    更新日期:2013-03-01 00:00:00

  • Controversial view of a genetically altered mouse model of focal retinal degeneration.

    abstract::Tuo et al. (2012) demonstrated tumor necrosis factor-inducible gene 6 recombinant protein (TSG-6) arrest of focal retinal lesions on a Ccl2 and Cx3cr1 double deficient mouse (DKO) on rd8 background (hereon referred to as DKO rd8). DKO rd8, a model of focal retinal degeneration with earlier onset and higher penetrance ...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.22949

    authors: Chu XK,Wang Y,Ardeljan D,Tuo J,Chan CC

    更新日期:2013-05-01 00:00:00

  • Bioengineered probiotics, a strategic approach to control enteric infections.

    abstract::Enteric infections account for high morbidity and mortality and are considered to be the fifth leading cause of death at all ages worldwide. Seventy percent of all enteric infections are foodborne. Thus significant efforts have been directed toward the detection, control and prevention of foodborne diseases. Many anti...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.4161/bioe.23574

    authors: Amalaradjou MA,Bhunia AK

    更新日期:2013-11-01 00:00:00

  • Gene gymnastics: Synthetic biology for baculovirus expression vector system engineering.

    abstract::Most essential activities in eukaryotic cells are catalyzed by large multiprotein assemblies containing up to ten or more interlocking subunits. The vast majority of these protein complexes are not easily accessible for high resolution studies aimed at unlocking their mechanisms, due to their low cellular abundance an...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.22966

    authors: Vijayachandran LS,Thimiri Govinda Raj DB,Edelweiss E,Gupta K,Maier J,Gordeliy V,Fitzgerald DJ,Berger I

    更新日期:2013-09-01 00:00:00

  • Combinatorial engineering for heterologous gene expression.

    abstract::Tools for strain engineering with predictable outcome are of crucial importance for the nascent field of synthetic biology. The success of combining different DNA biological parts is often restricted by poorly understood factors deriving from the complexity of the systems. We have previously identified variants for di...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.24703

    authors: Zwick F,Lale R,Valla S

    更新日期:2013-11-01 00:00:00

  • Putting bugs to the blush: metabolic engineering for phenylpropanoid-derived products in microorganisms.

    abstract::Phenylpropanoids and phenylpropanoid-derived phenolic compounds such as flavonoids, anthocyanins, or stilbenes are secondary plant metabolites which serve as pigments and scent compounds or provide protection against environmental stress. Due to their antioxidant properties they also have been widely recognized for th...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.23885

    authors: van Summeren-Wesenhagen PV,Marienhagen J

    更新日期:2013-11-01 00:00:00

  • Whole cell biotransformation for reductive amination reactions.

    abstract::Whole cell biotransformation systems with enzyme cascading increasingly find application in biocatalysis to complement or replace established chemical synthetic routes for production of, e.g., fine chemicals. Recently, we established an Escherichia coli whole cell biotransformation system for reductive amination by co...

    journal_title:Bioengineered

    pub_type: 评论,杂志文章

    doi:10.4161/bioe.27151

    authors: Klatte S,Lorenz E,Wendisch VF

    更新日期:2014-01-01 00:00:00

  • Addition of poly (propylene glycol) to multiblock copolymer to optimize siRNA delivery.

    abstract::Previous studies have examined different strategies for siRNA delivery with varying degrees of success. These include use of viral vectors, cationic liposomes, and polymers. Several copolymers were designed and synthesized based on blocks of poly(ethylene glycol) PEG, poly(propylene glycol) PPG, and poly(l-lysine). Th...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.27339

    authors: Dai Z,Arévalo MT,Li J,Zeng M

    更新日期:2014-01-01 00:00:00

  • Downstream processing of biopharmaceutical proteins produced in plants: the pros and cons of flocculants.

    abstract::All biological platforms for the manufacture of biopharmaceutical proteins produce an initially turbid extract that must be clarified to avoid fouling sensitive media such as chromatography resins. Clarification is more challenging if the feed stream contains large amounts of dispersed particles, because these rapidly...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.28061

    authors: Buyel JF,Fischer R

    更新日期:2014-03-01 00:00:00

  • Designing novel cellulase systems through agent-based modeling and global sensitivity analysis.

    abstract::Experimental techniques allow engineering of biological systems to modify functionality; however, there still remains a need to develop tools to prioritize targets for modification. In this study, agent-based modeling (ABM) was used to build stochastic models of complexed and non-complexed cellulose hydrolysis, includ...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.29160

    authors: Apte AA,Senger RS,Fong SS

    更新日期:2014-07-01 00:00:00

  • Experimental determination and characterization of the gap promoter of Bifidobacterium bifidum S17.

    abstract::The DNA sequence upstream of the glyceraldehyde 3-phosphate dehydrogenase gene (gap) of various strains of bifidobacteria is used in a number of vector systems for homologous and heterologous expression in this group of bacteria. To date none of the bifidobacterial gap promoters (Pgap) have been verified experimentall...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.34423

    authors: Sun Z,Westermann C,Yuan J,Riedel CU

    更新日期:2014-01-01 00:00:00

  • Pathway transfer in fungi.

    abstract::Itaconic acid is an important building block for the chemical industry. Currently, Aspergillus terreus is the main organism used for itaconic acid production. Due to the enormous citric acid production capacity of Aspergillus niger, this host is investigated as a potential itaconic acid production host. Several strate...

    journal_title:Bioengineered

    pub_type: 评论,杂志文章

    doi:10.4161/bioe.29936

    authors: van der Straat L,de Graaff LH

    更新日期:2014-09-01 00:00:00

  • Engineering Ashbya gossypii for efficient biolipid production.

    abstract::Ashbya gossypii is a filamentous fungus that naturally overproduces riboflavin. Indeed, engineered strains are currently used for the industrial production of riboflavin, replacing the chemical synthesis processes formerly used. The utilization of A. gossypii for biotechnological applications affords significant advan...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2015.1011525

    authors: Ledesma-Amaro R,Lozano-Martínez P,Jiménez A,Revuelta JL

    更新日期:2015-01-01 00:00:00

  • Enhanced expression of heterologous proteins in yeast cells via the modification of N-glycosylation sites.

    abstract::Yeasts are widely used for the production of heterologous proteins. Improving the expression of such proteins is a top priority for pharmaceutical and industrial applications. N-Glycosylation, a common form of protein modification in yeasts, facilitates proper protein folding and secretion. Accordingly, our previous s...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2015.1011031

    authors: Han M,Yu X

    更新日期:2015-01-01 00:00:00

  • Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications.

    abstract::Alginate lyases catalyze the degradation of alginate, a complex copolymer of α-L-guluronate and its C5 epimer β-D-mannuronate. The enzymes have been isolated from various kinds of organisms with different substrate specificities, including algae, marine mollusks, marine and terrestrial bacteria, and some viruses and f...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.1080/21655979.2015.1030543

    authors: Zhu B,Yin H

    更新日期:2015-01-01 00:00:00

  • Mechanical characterization of the rat and mice skin tissues using histostructural and uniaxial data.

    abstract::The skin tissue has been shown to behave like a nonlinear anisotropic material. This study was aimed to employ a constitutive fiber family equation to characterize the nonlinear anisotropic mechanical behavior of the rat and mice skin tissues in different anatomical locations, including the abdomen and back, using his...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2015.1036202

    authors: Karimi A,Rahmati SM,Navidbakhsh M

    更新日期:2015-01-01 00:00:00

  • Bacterial d-amino acid oxidases: Recent findings and future perspectives.

    abstract::D-Amino acid oxidase (DAO) is a flavin enzyme that catalyzes the oxidative deamination of d-amino acids. This enzyme has been studied extensively both biochemically and structurally as a model for the oxidase-dehydrogenase class of flavoproteins. This enzyme also has various applications, such as the determination of ...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.1080/21655979.2015.1052917

    authors: Takahashi S,Abe K,Kera Y

    更新日期:2015-01-01 00:00:00

  • Under the microscope: From pathogens to probiotics and back.

    abstract::The review centers on the human gastrointestinal tract; focusing first on the bacterial stress responses needed to overcome the physiochemical defenses of the host, specifically how these stress survival strategies can be used as targets for alternative infection control strategies. The concluding section focuses on r...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.1080/21655979.2015.1089368

    authors: Sleator RD

    更新日期:2015-01-01 00:00:00

  • Novel application of bacteriophage for controlling foaming in wastewater treatment plant- an eco-friendly approach.

    abstract::This addendum to "Novel application of bacteriophage for controlling foaming in wastewater treatment plant- an eco-friendly approach " includes characteristics of the phages NOC1, NOC2 and NOC3 not discussed in the previous paper. The phage adsorption and host interaction properties, their sensitivity to pH and temper...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2015.1134066

    authors: Khairnar K,Chandekar R,Nair A,Pal P,Paunikar WN

    更新日期:2016-01-01 00:00:00

  • MicroRNA, a new target for engineering new crop cultivars.

    abstract::Global sustainable development depends on, at least partially, the sustainable development of crop reproduction that provides food, cloth and bioenergy as well certain drugs. During thousands of years of human history, many crops have been domesticated for feeding the world. Perfectly, in the past 2 decades, scientist...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1141838

    authors: Zhang B,Wang Q

    更新日期:2016-01-01 00:00:00

  • Recent advances in the production of recombinant subunit vaccines in Pichia pastoris.

    abstract::Recombinant protein subunit vaccines are formulated using defined protein antigens that can be produced in heterologous expression systems. The methylotrophic yeast Pichia pastoris has become an important host system for the production of recombinant subunit vaccines. Although many basic elements of P. pastoris expres...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.1080/21655979.2016.1191707

    authors: Wang M,Jiang S,Wang Y

    更新日期:2016-04-01 00:00:00

  • Optimization of Lipase production from a novel strain Thalassospira permensis M35-15 using Response Surface Methodology.

    abstract::Lipases can catalyze the hydrolysis of glycerol, esters and long chain fatty acids. A lipase producing isolate M35-15 was screened and identified as Thalassospira permensis using 16S rRNA gene sequence analysis. To our knowledge this is the first report on Thalassospira permensis producing lipases. In this paper the o...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1197713

    authors: Kai W,Peisheng Y

    更新日期:2016-09-02 00:00:00

  • Quantitative analysis of multiple biokinetic models using a dynamic water phantom: A feasibility study.

    abstract::This study analyzed multiple biokinetic models using a dynamic water phantom. The phantom was custom-made with acrylic materials to model metabolic mechanisms in the human body. It had 4 spherical chambers of different sizes, connected by 8 ditches to form a complex and adjustable water loop. One infusion and drain po...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1197738

    authors: Chiang FT,Li PJ,Chung SP,Pan LF,Pan LK

    更新日期:2016-09-02 00:00:00

  • Effects of protein and phosphate buffer concentrations on thermal denaturation of lysozyme analyzed by isoconversional method.

    abstract::Thermal denaturation of lysozymes was studied as a function of protein concentration, phosphate buffer concentration, and scan rate using differential scanning calorimetry (DSC), which was then analyzed by the isoconversional method. The results showed that lysozyme thermal denaturation was only slightly affected by t...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1197629

    authors: Cao XM,Tian Y,Wang ZY,Liu YW,Wang CX

    更新日期:2016-07-03 00:00:00

  • Effective ethanol production from whey powder through immobilized E. coli expressing Vitreoscilla hemoglobin.

    abstract::Ethanol production from whey powder was investigated by using free as well as alginate immobilized E. coli and E. coli expressing Vitreoscilla hemoglobin (VHb) in both shake flask and fermenter cultures. Media with varying levels of whey (lactose contents of 3%, 5%, 8% or 15%) and yeast extract (0.3% or 0.5%) were eva...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1218581

    authors: Sar T,Stark BC,Yesilcimen Akbas M

    更新日期:2017-03-04 00:00:00

  • Ex vivo comparative study on three sinus lift tools for transcrestal detaching maxillary sinus mucosa.

    abstract::The objective of this study was to comparatively evaluate 3 different sinus lift tools, namely umbrella-shaped sinus lift curette YSL-04, our recently designed probe-improved sinus lift curettes, and our newly invented elevator 014, using our previous developed goat ex vivo models for direct visualizing the effectiven...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1228497

    authors: Li Y,Hu P,Han Y,Fan J,Dong X,Ren H,Yang C,Shi T,Xia D

    更新日期:2017-07-04 00:00:00

  • Clinicopathological significance of survivin expression in patients with cervical cancer: A systematic meta-analysis.

    abstract:BACKGROUND:Survivin has been shown to play an important role in cancer pathogenesis. However, its role in cervical cancer development is still controversial. This study was performed to evaluate the clinical significance of survivin expression in cervical cancer. METHODS:Search of some online electronic databases was ...

    journal_title:Bioengineered

    pub_type: 杂志文章,meta分析

    doi:10.1080/21655979.2016.1252879

    authors: Fan Y,Chen J

    更新日期:2017-09-03 00:00:00