Polyphasic characterization of rhizobia that nodulate Phaseolus vulgaris in West Africa (Senegal and Gambia).

Abstract:

:Fifty-eight new isolates were obtained from root nodules of common bean (Phaseolus vulgaris) cultivated in soils originating from different agroecological areas in Senegal and Gambia (West Africa). A polyphasic approach including both phenotypic and genotypic techniques was used to study the diversity of the 58 Rhizobium isolates and to determine their taxonomic relationships with reference strains. All the techniques performed, analysis of multilocus enzyme electrophoretic patterns, SDS-PAGE profiles of total cell proteins, PCR-RFLP analysis of the genes encoding 16S rRNA and of the 16S-23S RNA intergenic spacer region (ITS-PCR-RFLP), auxanographic tests using API galleries and nodulation tests lead to the consensus conclusion that the new rhizobial isolates formed two main distinct groups, I and II, belonging to Rhizobium tropici type B and Rhizobium etli, respectively. By MLEE R. etli and group II strains showed several related electrophoretic types, evidencing some extent of internal heterogeneity among them. This heterogeneity was confirmed by other techniques (ITS-PCR-RFLP, SDS-PAGE and host-plant-specificity) with the same nine distinct strains of group II showing some differences from the core of group II (54 strains).

authors

Diouf A,de Lajudie P,Neyra M,Kersters K,Gillis M,Martinez-Romero E,Gueye M

doi

10.1099/00207713-50-1-159

keywords:

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

159-170

eissn

1466-5026

issn

1466-5034

journal_volume

50 Pt 1

pub_type

杂志文章
  • Pseudonocardia oroxyli sp. nov., a novel actinomycete isolated from surface-sterilized Oroxylum indicum root.

    abstract::A high-G+C-content, Gram-positive bacterium, strain D10(T), was isolated from the root of Oroxylum indicum, a Chinese medicinal plant. Based on 16S rRNA gene sequence analysis, strain D10(T) was a member of the genus Pseudonocardia and was most closely related, albeit loosely, to Pseudonocardia halophobica. Morphologi...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64385-0

    authors: Gu Q,Luo H,Zheng W,Liu Z,Huang Y

    更新日期:2006-09-01 00:00:00

  • Reclassification and emended description of Caulobacter leidyi as Sphingomonas leidyi comb. nov., and emendation of the genus Sphingomonas.

    abstract::'Caulobacter leidyi' DSM 4733(T) has been shown to be affiliated with the family Sphingomonadaceae instead of the Caulobacteraceae, and due to its poor characterization has been omitted from the current edition of Bergey's Manual of Systematic Bacteriology and removed to limbo. We isolated a novel sphingoglycolipid-co...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.039636-0

    authors: Chen H,Jogler M,Rohde M,Klenk HP,Busse HJ,Tindall BJ,Spröer C,Overmann J

    更新日期:2012-12-01 00:00:00

  • Lactobacillus pobuzihii sp. nov., isolated from pobuzihi (fermented cummingcordia).

    abstract::Twenty-one homofermentative lactic acid bacteria were isolated from fermented cummingcordia (pobuzihi), a traditional food in Taiwan. The isolates had identical 16S rRNA gene sequences that were distinct from those of other lactobacilli, and their closest neighbours in the 16S rRNA gene sequence phylogenetic tree were...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.016873-0

    authors: Chen YS,Miyashita M,Suzuki KI,Sato H,Hsu JS,Yanagida F

    更新日期:2010-08-01 00:00:00

  • Roseibacterium elongatum gen. nov., sp. nov., an aerobic, bacteriochlorophyll-containing bacterium isolated from the west coast of Australia.

    abstract::A novel aerobic, chemoheterotrophic, bacteriochlorophyll-containing bacterium, strain OCh 323T, was isolated from sand at Monkey Mia, Shark Bay, located on the west coast of Australia. The cells were Gram-negative, non-motile rods of variable length; one or both cell poles was narrower. Bacteriochlorophyll a was synth...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.02094-0

    authors: Suzuki T,Mori Y,Nishimura Y

    更新日期:2006-02-01 00:00:00

  • Reclassification of Phycicola gilvus (Lee et al. 2008) and Leifsonia pindariensis (Reddy et al. 2008) as Microterricola gilva comb. nov. and Microterricola pindariensis comb. nov. and emended description of the genus Microterricola.

    abstract::The taxonomic positions of Microterricola viridarii JCM 15926T, Phycicola gilvus DSM 18319T and Leifsonia pindariensis JCM 15132T were re-examined. Phylogenetic analysis and 16S rRNA gene sequence similarities revealed that all three strains are closely related with each other and form a monophyletic cluster with high...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002019

    authors: Dhotre DP,Rajabal V,Sharma A,Kulkarni GJ,Prakash O,Vemuluri VR,Joseph N,Rahi P,Shouche YS

    更新日期:2017-08-01 00:00:00

  • Pontibacter amylolyticus sp. nov., isolated from a deep-sea sediment hydrothermal vent field.

    abstract::A Gram-stain-negative, short rod-shaped bacterium, designated 9-2T, was isolated from a sediment sample collected from a hydrothermal vent field on the south-west Indian Ridge. It formed red colonies, produced carotenoid-like pigments and did not produce bacteriochlorophyll a. Strain 9-2T was positive for hydrolysis o...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000944

    authors: Wu YH,Zhou P,Jian SL,Liu ZS,Wang CS,Oren A,Xu XW

    更新日期:2016-04-01 00:00:00

  • Enterococcus hermanniensis sp. nov., from modified-atmosphere-packaged broiler meat and canine tonsils.

    abstract::Isolates 302, 334, 356, 377 and 379, detected in modified-atmosphere-packaged broiler meat, together with strains LMG 12317T and LMG 13617, detected in dog tonsils, were analysed in a polyphasic taxonomy study, including numerical analysis of ribopatterns and whole-cell protein patterns, 16S rRNA gene sequence analysi...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63112-0

    authors: Koort J,Coenye T,Vandamme P,Sukura A,Björkroth J

    更新日期:2004-09-01 00:00:00

  • Haloarchaeobius salinus sp. nov., isolated from an inland salt lake, and emended description of the genus Haloarchaeobius.

    abstract::The halophilic archaeal strain, YC82(T), was isolated from Yuncheng salt lake in Shanxi, PR China. Cells from strain YC82(T) were Gram-stain negative, pleomorphic rods, which lysed in distilled water and formed light-red colonies on solid media. Strain YC82(T) grew at 25-50 °C (optimum 37 °C), in 1.4-4.8 M NaCl (optim...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.000038

    authors: Yuan PP,Zhang WJ,Han D,Cui HL

    更新日期:2015-03-01 00:00:00

  • Actinomadura rhizosphaerae sp. nov., isolated from rhizosphere soil of the plant Azadirachta indica.

    abstract::A novel actinomycete, strain SDA37T, belonging to the genus Actinomadura, was isolated from rhizosphere soil collected from Udon Thani Province, Thailand. The taxonomic position of the strain was characterized using a polyphasic approach. Meso-diaminopimelic acid, glucose, ribose, galactose and madurose were detected ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002940

    authors: Malisorn K,Kanchanasin P,Phongsopitanun W,Tanasupawat S

    更新日期:2018-09-01 00:00:00

  • Devriesea agamarum gen. nov., sp. nov., a novel actinobacterium associated with dermatitis and septicaemia in agamid lizards.

    abstract::Five bacterial isolates were recovered from dermatitis or organ lesions of five agamid lizards. Three strains were recovered from Uromastyx species with dermatitis or septicaemia. All five isolates were Gram-positive small rods that could not be identified using conventional phenotypic systems. They grew on sheep bloo...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65478-0

    authors: Martel A,Pasmans F,Hellebuyck T,Haesebrouck F,Vandamme P

    更新日期:2008-09-01 00:00:00

  • Brevibacterium daeguense sp. nov., a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture.

    abstract::A Gram-reaction-positive, non-spore-forming, aerobic actinobacterial strain (2C6-41(T)) was isolated from the activated sludge from an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.038141-0

    authors: Cui Y,Kang MS,Woo SG,Jin L,Kim KK,Park J,Lee M,Lee ST

    更新日期:2013-01-01 00:00:00

  • Nomenclature and taxonomy of the genus Salmonella.

    abstract::The nomenclature of the genus Salmonella has reached an unsatisfactory state of affairs, with two systems of nomenclature in circulation. One system, proposed in the 1980s by Le Minor and Popoff, has received wide acceptance, although it does not conform to the rules of the Bacteriological Code. The other system, whic...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 评论,杂志文章

    doi:10.1099/ijs.0.63580-0

    authors: Tindall BJ,Grimont PAD,Garrity GM,Euzéby JP

    更新日期:2005-01-01 00:00:00

  • Flavobacterium luticocti sp. nov., isolated from wastewater.

    abstract::A Gram-stain-negative, non-spore-forming, rod-shaped and non-motile bacterium, designated strain xz20T, was isolated from the wastewater of a municipal wastewater treatment plant in Xuzhou, China. The taxonomic status of strain xz20T was determined using a polyphasic approach. Growth occurred at 15-40 °C (optimum, 25-...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001634

    authors: Liu H,Lu P,Jin L,Zhu G

    更新日期:2017-02-01 00:00:00

  • Chryseobacterium ginsengisoli sp. nov., isolated from the rhizosphere of ginseng and emended description of Chryseobacterium gleum.

    abstract::Strain DCY 63(T), isolated from the rhizosphere of ginseng, was a Gram-reaction-negative, strictly aerobic, non-motile, catalase- and oxidase-positive rod. 16S rRNA gene sequence analysis revealed that strain DCY 63(T) belonged to the genus Chryseobacterium and was closely related to Chryseobacterium indoltheticum LMG...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.045427-0

    authors: Nguyen NL,Kim YJ,Hoang VA,Yang DC

    更新日期:2013-08-01 00:00:00

  • Brachybacterium hainanense sp. nov., isolated from noni (Morinda citrifolia L.) branch.

    abstract::A Gram-stain-positive bacterial strain, designated as NR2T, isolated from noni (Morinda citrifolia L.) branch was investigated using a polyphasic taxonomic approach. The cells were small coccoid to ovoid, non-spore-forming and motile. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was a ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000559

    authors: Liu Y,Zhai L,Yao S,Cao Y,Cao Y,Zhang X,Su J,Ge Y,Zhao R,Cheng C

    更新日期:2015-11-01 00:00:00

  • Fervidicola ferrireducens gen. nov., sp. nov., a thermophilic anaerobic bacterium from geothermal waters of the Great Artesian Basin, Australia.

    abstract::A strictly anaerobic, thermophilic bacterium, designated strain Y170(T), was isolated from a microbial mat colonizing thermal waters of a run-off channel created by the free-flowing waters of a Great Artesian Basin (GAB) bore well (New Lorne bore; registered number 17263). Cells of strain Y170(T) were slightly curved ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.004200-0

    authors: Ogg CD,Patel BK

    更新日期:2009-05-01 00:00:00

  • Ramlibacter alkalitolerans sp. nov., alkali-tolerant bacterium isolated from soil of ginseng.

    abstract::A novel bacterial strain, designated CJ661T, was isolated from soil of ginseng in Anseong, South Korea. Cells of strain CJ661T were white-coloured, Gram-staining-negative, non-motile, aerobic and rod-shaped. Strain CJ661T grew optimally at 30 °C and pH 7.0. The analysis of 16S rRNA gene sequence of strain CJ661T showe...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002342

    authors: Lee DH,Cha CJ

    更新日期:2017-11-01 00:00:00

  • Jiella pacifica sp. nov., isolated from the West Pacific Ocean.

    abstract::A Gram-stain-negative bacterium, designated strain 40Bstr34T, isolated from a sediment sample from the West Pacific Ocean, was taxonomically characterized by using a polyphasic approach. The strain was phylogenetically close to Jiella aquimaris LZB041T and Jiella endophytica CBS5Q-3T, with 16S rRNA gene sequence simil...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004295

    authors: Xue Z,Zhu S,Chen X,Chen T,Ren N,Chen Y,Dong B,Shen X,Huang Y,Yang J,Chen J

    更新日期:2020-07-01 00:00:00

  • Vitellibacter nionensis sp. nov., isolated from a shallow water hydrothermal vent.

    abstract::A novel, Gram-stain-negative, non-motile, rod-shaped yellow bacterium, designated VBW088(T) was isolated from a shallow water hydrothermal vent in Espalamaca in the Azores. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain VBW088(T) clustered with three type strains of species of the genus Vite...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.070805-0

    authors: Rajasabapathy R,Mohandass C,Yoon JH,Dastager SG,Liu Q,Khieu TN,Son CK,Li WJ,Colaço A

    更新日期:2015-02-01 00:00:00

  • Hymenobacter psychrophilus sp. nov., a psychrophilic bacterium isolated from soil.

    abstract::A non-motile, rod-shaped, red-pigmented bacterium, designated strain BZ33r(T), was isolated from soil of an industrial site. Cells stained Gram-negative and were aerobic and psychrophilic, showing good growth at 1-20 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ33r(T) was related to ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.023465-0

    authors: Zhang DC,Busse HJ,Liu HC,Zhou YG,Schinner F,Margesin R

    更新日期:2011-04-01 00:00:00

  • Investigation of taxa of the family Pasteurellaceae isolated from Syrian and European hamsters and proposal of Mesocricetibacter intestinalis gen. nov., sp. nov. and Cricetibacter osteomyelitidis gen. nov., sp. nov.

    abstract::Eleven strains from hamster of Bisgaard taxa 23 and 24, also referred to as Krause's groups 2 and 1, respectively, were investigated by a polyphasic approach including data published previously. Strains showed small, regular and circular colonies with smooth and shiny appearance, typical of members of the family Paste...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.067470-0

    authors: Christensen H,Nicklas W,Bisgaard M

    更新日期:2014-11-01 00:00:00

  • Micromonospora sediminis sp. nov., isolated from mangrove sediment.

    abstract::The novel actinomycete, strain CH3-3T, was isolated from mangrove sediment collected from Chonburi Province, Thailand. On the basis of its spore morphology and chemotaxonomic characteristics, the strain belonged to the genus Micromonospora. It contained meso-diaminopimelic acid, glucose, mannose, xylose, ribose and rh...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001175

    authors: Phongsopitanun W,Kudo T,Ohkuma M,Pittayakhajonwut P,Suwanborirux K,Tanasupawat S

    更新日期:2016-08-01 00:00:00

  • Zavarzinia aquatilis sp. nov., isolated from a freshwater river.

    abstract::A Gram-stain-negative, strictly aerobic, catalase-positive and oxidase-positive bacterium, designated strain HR-AST, was isolated from a water sample of the Han River located in the Republic of Korea. Cells were motile rods with a polar flagellum. Growth was observed at 5-35 °C (optimum of 25 °C) and pH 6-8 (optimum o...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003214

    authors: Lee Y,Park HY,Jeon CO

    更新日期:2019-03-01 00:00:00

  • Phylogenetic relationships amongst tetrahymenine ciliates inferred by a comparison of telomerase RNAs.

    abstract::The phylogenetic relationships between ciliate species in the suborder Tetrahymenina, order Hymenostomata, was investigated by comparing their telomerase RNA (TER) sequences. This relatively small RNA is an integral part of telomerase, the ribonucleoprotein enzyme that catalyses the synthesis of telomeric DNA. Despite...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-52-6-2297

    authors: Ye AJ,Romero DP

    更新日期:2002-11-01 00:00:00

  • Proposal of 'Candidatus Frankia alpina', the uncultured symbiont of Alnus alnobetula and A. incana that forms spore-containing nitrogen-fixing root nodules.

    abstract::The members of the genus Frankia are, with a few exceptions, a group of nitrogen-fixing symbiotic actinobacteria that nodulate mostly woody dicotyledonous plants belonging to three orders, eight families and 23 genera of pioneer dicots. These bacteria have been characterized phylogenetically and grouped into four mole...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004433

    authors: Pozzi ACM,Herrera-Belaroussi A,Schwob G,Bautista-Guerrero HH,Bethencourt L,Fournier P,Dubost A,Abrouk D,Normand P,Fernandez MP

    更新日期:2020-10-01 00:00:00

  • Candida olivae sp. nov., a novel yeast species from 'Greek-style' black olive fermentation.

    abstract::Two yeast strains (FMCC Y-1(T) and FMCC Y-2) were recovered during a survey of the yeast biota associated with fermenting black olives, collected from an olive tree (Olea europaea L. cv. 'Conservolea') orchard in Central Greece. Phylogenetic analysis based on rRNA gene sequences (18S, 26S, and 5.8S-ITS) indicated that...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.012955-0

    authors: Nisiotou AA,Panagou EZ,Nychas GE

    更新日期:2010-05-01 00:00:00

  • Flavobacterium collinsense sp. nov., isolated from a till sample of an Antarctic glacier.

    abstract::A novel rod-shaped, Gram-stain-negative, non-gliding and aerobic strain surrounded by a multilayer capsule, designated 4-T-2T, was isolated from a till sample of Collins glacier front, Antarctica. The bacterium formed yellow, circular, convex and smooth colonies. Growth occurred at 4-28 °C (optimum18-20 °C), at pH 7.0...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000688

    authors: Zhang Y,Jiang F,Chang X,Qiu X,Ren L,Qu Z,Deng S,Da X,Fang C,Peng F

    更新日期:2016-01-01 00:00:00

  • Halioglobus sediminis sp. nov., isolated from coastal sediment.

    abstract::A novel aerobic, Gram-stain-negative, non-motile and rod-shaped bacterium, designated strain U0301T, was isolated from a marine sediment sample in Weihai, China. This strain grew optimally at 33 °C, pH 7.5 and in the presence of 2.0-3.0 % (w/v) NaCl. Strain U0301T shared pairwise 16S rRNA gene sequence similarities of...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003366

    authors: Han JR,Ye MQ,Wang C,Du ZJ

    更新日期:2019-06-01 00:00:00

  • Phenotypic variability and phylogenetic relationships of the genera Tolypothrix and Calothrix (Nostocales, Cyanobacteria) from running water.

    abstract::The taxonomy of heterocystous cyanobacteria belonging to the genera Calothrix and Tolypothrix has long been a matter of debate, but their phylogenetic relationships are still not well understood. Our aim was to compare the phylogeny and morphology of members of these genera, which exhibit basal-apical polarity. A phyl...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.027581-0

    authors: Berrendero E,Perona E,Mateo P

    更新日期:2011-12-01 00:00:00

  • Xuhuaishuia manganoxidans gen. nov., sp. nov., a manganese-oxidizing bacterium isolated from deep-sea sediments from the Pacific Polymetallic Nodule Province.

    abstract::A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped, manganese-oxidizing bacterial strain, designated DY6-4T, was isolated from the surface sediment of the Pacific Clarion-Clipperton Fracture Zone. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain DY6-4T formed a lineage within...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000912

    authors: Wang L,Liu Y,Shi X,Wang Y,Zheng Y,Dai X,Zhang XH

    更新日期:2016-03-01 00:00:00