Abstract:
:The mitochondrial outer membrane separates the intermembrane space from the cytosol. The whole exchange of metabolites, cations and information between mitochondria and the cell occurs through the outer membrane. Experimental evidence is reviewed supporting the hypothesis of dynamic ADP compartmentation within the intermembrane space. The outer membrane creates a diffusion barrier for small molecules (adenine nucleotides, creatine phosphate, creatine etc.) causing rate-dependent concentration gradients as a prerequisite for the action of ADP shuttles via creatine kinases or adenylate kinases. If the outer membrane becomes leaky, cytochrome c and apoptosis-inducing factor can be released, leading to apoptosis, and as a bioenergetic consequence the cytosolic phosphorylation potential decreases. Leaky outer membranes can be detected in saponin-skinned fibres with spectrophotometric and oxygraphic methods. This is of special interest in respect to acute impairment of mitochondria during ischaemia/reperfusion.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Gellerich FN,Trumbeckaite S,Opalka JR,Seppet E,Rasmussen HN,Neuhoff C,Zierz Sdoi
10.1042/bst0280164keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
164-9issue
2eissn
0300-5127issn
1470-8752journal_volume
28pub_type
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