Involvement of ArcA and Fnr in expression of Escherichia coli thiol peroxidase gene.

Abstract:

:To explore the oxygen response regulators involved in thiol peroxidase gene (tpx) expression in Escherichia coli, we constructed a single-copy tpx-lacZ operon fusion and monitored tpx-lacZ expression in various genetic backgrounds. Expression of the tpx-lacZ fusion was increased 4-fold by aerobic growth. Anaerobic expression of tpx-lacZ in either (delta)arcA or delta(fnr) strains was 2.5-fold depressed compared with that of the wild-type strain. The results of immunoblotting experiments also demonstrated that ArcA and Fnr regulatory proteins repressed thiol peroxidase gene expression during anaerobic growth. Inspection of the tpx promoter region revealed putative binding sites for ArcA and Fnr. It thus appears that ArcA and Fnr function as repressors by blocking the binding of RNA polymerase to the tpx promoter in E. coli under anaerobic growth conditions.

journal_name

IUBMB Life

journal_title

IUBMB life

authors

Kim SJ,Han YH,Kim IH,Kim HK

doi

10.1080/713803496

keywords:

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

215-8

issue

2

eissn

1521-6543

issn

1521-6551

journal_volume

48

pub_type

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