Abstract:
:To explore the oxygen response regulators involved in thiol peroxidase gene (tpx) expression in Escherichia coli, we constructed a single-copy tpx-lacZ operon fusion and monitored tpx-lacZ expression in various genetic backgrounds. Expression of the tpx-lacZ fusion was increased 4-fold by aerobic growth. Anaerobic expression of tpx-lacZ in either (delta)arcA or delta(fnr) strains was 2.5-fold depressed compared with that of the wild-type strain. The results of immunoblotting experiments also demonstrated that ArcA and Fnr regulatory proteins repressed thiol peroxidase gene expression during anaerobic growth. Inspection of the tpx promoter region revealed putative binding sites for ArcA and Fnr. It thus appears that ArcA and Fnr function as repressors by blocking the binding of RNA polymerase to the tpx promoter in E. coli under anaerobic growth conditions.
journal_name
IUBMB Lifejournal_title
IUBMB lifeauthors
Kim SJ,Han YH,Kim IH,Kim HKdoi
10.1080/713803496keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
215-8issue
2eissn
1521-6543issn
1521-6551journal_volume
48pub_type
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