Abstract:
:The metabolism of paraquat generates oxygen radicals. Paraquat has thus been suggested as a model compound to induce oxidative damage to DNA, lipids and proteins in different cells and tissues, although experimental data are inconsistent. In order to explore the possibilities for an animal model of oxidative DNA damage in vivo, rats were treated with 20 mg/kg paraquat or vehicle i.p. One and five days later we measured DNA oxidation in terms of 7-hydro-8-oxo-2'-deoxyguanosine (8-oxodG) in the liver and lung as well as the urinary excretion of 8-oxodG. No significant effects on the level of 8-oxodG in the liver, the lung or the urinary excretion, could be distinguished following paraquat treatment. We found, however, a significant correlation (r = 0.69; p<0.0002) between the 8-oxodG level in the lung and the urinary excretion, but no significant correlation between the level in the liver and the urinary excretion or between the levels in the liver and the lung. During the experiment the rats were clearly affected by the paraquat as they were very lethargic compared to the controls. Accordingly, even at toxic doses, paraquat did not cause detectable oxidative damage to DNA. The data do not support the use of paraquat as a model compound in experiments investigating effects or prevention of oxidative damage to DNA.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Sørensen M,Loft Sdoi
10.1080/10715760000300421keywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
423-8issue
5eissn
1071-5762issn
1029-2470journal_volume
32pub_type
杂志文章abstract::Proteins constitute the major 'working force' for all forms of biological work. Their exact conformation and pattern of folding are tightly connected to their activity and function. Reactive oxygen and nitrogen species (ROS and RNS) are formed during normal metabolism and in higher fluxes under pathological conditions...
journal_title:Free radical research
pub_type: 杂志文章,评审
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journal_title:Free radical research
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journal_title:Free radical research
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journal_title:Free radical research
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doi:10.1080/10715760902942824
更新日期:2009-07-01 00:00:00
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journal_title:Free radical research
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更新日期:2012-10-01 00:00:00
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journal_title:Free radical research
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更新日期:2004-08-01 00:00:00
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doi:10.1080/10715760000300681
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doi:10.1080/10715760903164998
更新日期:2009-10-01 00:00:00
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doi:10.3109/10715769609088034
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journal_title:Free radical research
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更新日期:1995-04-01 00:00:00
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journal_title:Free radical research
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更新日期:2007-11-01 00:00:00
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