Abstract：
AIM:To investigate whether there is loss of the p27Kip1 protein in developing cervical cancer and whether p27Kip1 immunoreactivity has any relation to the proliferative indicator Ki-67. METHODS:The expression of p27Kip1 and Ki-67 was assessed by immunohistochemistry in serial sections from normal epithelium (13), low grade (27) and high grade (19) squamous intraepithelial lesions (LSIL, HSIL), and invasive cervical cancer (23). In the SIL cases the presence of human papillomavirus (HPV) genomic sequences was assessed by in situ hybridisation. The results were evaluated by image analysis, and reported as mean score of the percentage of p27Kip1 and of Ki-67 positive cells in each histological group. RESULTS:In general, p27Kip1 immunostaining was related to squamous differentation, and was intense in normal epithelium (47%), while it was reduced in SIL lesions as an effect of the decreased number of differentiating cells. However, decrease in the p27Kip1 expression was more evident in LSIL (36%) than in HSIL (39%); in the latter, p27Kip1 had a different intraepithelial distribution in that the staining extended to the basal cells. The average levels of p27Kip1 were similar in SIL lesions associated to low, intermediate, and high risk HPV types. Compared with normal epithelium and dysplasia, invasive cancer showed significantly lower p27Kip1 levels (23%). There was no relation between p27Kip1 and Ki-67 labelling indices in any of the histological groups examined. CONCLUSIONS:A reduction in p27Kip1 protein occurs in cervical cancer independently of the proliferative status. The changes in p27Kip1 expression may be related to the unregulated kinetics of developing cervical cancer.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Troncone G,Vetrani A,de Rosa G,Gerbasio D,Palombini Ldoi
10.1136/jcp.52.12.880keywords:
subject
Has Abstractpub_date
1999-12-01 00:00:00pages
880-7issue
12eissn
0021-9746issn
1472-4146journal_volume
52pub_type
杂志文章abstract：:Synovium was collected from 15 patients who were undergoing joint surgery. Two groups were defined by clinical diagnosis: patients with primary osteoarthritis (n = 4); and those with rheumatoid arthritis (n = 11). The synovium was studied using histological and morphometric techniques. In agreement with previous studi...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.41.8.847
更新日期：1988-08-01 00:00:00
abstract：:A case of Cryptococcus neoformans meningitis is described in an HIV negative patient with undiagnosed systemic sarcoidosis. The patient presented with signs of meningitis together with generalised lymphadenopathy and hepatosplenomegaly. Cryptococcal meningitis was diagnosed on lumbar puncture. She was treated with int...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.52.12.928
更新日期：1999-12-01 00:00:00
abstract：:One hundred and seventyone antenatal patients were examined for the presence of ;large colony' mycoplasmas in the vagina, and for complement-fixing antibody to Mycoplasma hominis. In 25 patients the findings before and after delivery were compared. In patients from whom M. hominis was grown, antibody was twice as comm...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.20.4.633
更新日期：1967-07-01 00:00:00
abstract：AIMS:To determine the causes of ovarian granulomatous inflammation and to discuss the differential diagnoses. METHODS:The pathological features of all ovarian granulomas identified by pathology SNOMED coding in Northern Ireland over a 13 year period were reviewed. Case notes of patients were also reviewed. RESULTS:Th...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.50.4.324
更新日期：1997-04-01 00:00:00
abstract：:A unique case of a metastatic non-desmoplastic sarcomatoid malignant melanoma in an axillary lymph node showing smooth muscle differentiation in a 54 year old woman is described. The tumour cells exhibited alpha-smooth muscle actin, HHF-35 and desmin positivity but were negative for S100 protein and HMB-45. Ultrastruc...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.49.11.950
更新日期：1996-11-01 00:00:00
abstract：:A latex enzyme immunoassay (LEIA) for detecting IgG class antibody to rubella virus was compared with the latex agglutination test (LA) and a haemagglutination inhibition assay (HI). Of 243 sera tested, four discrepant results were observed among all three techniques, corresponding to borderline values. Except for one...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.43.9.766
更新日期：1990-09-01 00:00:00
abstract：:Incomplete expression of human haemoglobin beta-chains at birth may lead to difficulty in the early demonstration of an inherited beta-chain variant. In this case, the rare unstable variant, Hb Tacoma beta30 (B12) arg leads to ser, although not present in cord blood in sufficient amounts to be easily detected by routi...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.31.9.883
更新日期：1978-09-01 00:00:00
abstract：AIMS:Several studies have reported conflicting and inconclusive results concerning the clinical relevance of mucin expression in gastric carcinoma. This study investigated the correlations between aberrant expression of mucins in gastric carcinoma and patient clinicopathological features. METHODS:The expression of MUC...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.56.5.378
更新日期：2003-05-01 00:00:00
abstract：:Rabbit antiserum, elicited by the intravenous injection of a strain of Campylobacter jejuni heated to 100 degrees C, cross reacted strongly with all other thermophilic campylobacters tested as well as with "C pyloridis" and could be detected by indirect fluorescence with labelled anti-rabbit serum. Antisera to formali...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.37.9.1007
更新日期：1984-09-01 00:00:00
abstract：:One hundred cases of transitional cell carcinoma of the bladder were studied to determine whether squamous metaplasia and other histological features within the bladder can be of value in predicting outcome of treatment with radiotherapy. Sixty cases showed the changes of squamous metaplasia, and of this group 46 (78%...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.42.3.250
更新日期：1989-03-01 00:00:00
abstract：:Myelomatosis was diagnosed in a 64 year old man on the basis of a serum paraprotein band (type IgG lambda, 42 g/l), plasma cell infiltration of bone marrow, and multiple lytic lesions evident on skull x ray picture. Blood specimens taken into plain glass tubes showed bulky gelatinous clot formation with minimal clot r...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.47.3.266
更新日期：1994-03-01 00:00:00
abstract：:A patient with multiple myeloma had antigenically related monoclonal Fc-gamma fragments and complete IgG-kappa molecules in the serum. The urine contained only Fc-gamma fragments in the absence of Bence-Jones protein. The two distinct M-components in the serum showed electrophoretic identity but could be separated by ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.33.10.936
更新日期：1980-10-01 00:00:00
abstract：:The occurrence of a severe generalized haemorrhagic state in an obstetric case due to a heparin-like anticoagulant is described. This appeared in the post-partum period apparently following a compatible blood transfusion. The pattern of results found in conventional laboratory tests for elucidating acute blood coagula...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.16.2.108
更新日期：1963-03-01 00:00:00
abstract：:An indirect method of an enzyme-linked immunosorbent assay (ELISA) is described to assess the reactivity of antisera used for the identification of peptide hormone producing cells by immunocytochemistry. Compared with radioimmunoassay and immunodiffusion, the ELISA method has the advantages of simplicity and sensitivi...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.36.4.406
更新日期：1983-04-01 00:00:00
abstract：INTRODUCTION:Cellular blue naevi (CBN) measure 1-2 cm in diameter and affect the dermis, occasionally extending into the subcutaneous fat. The case of a 14-year-old boy with a giant CBN (GCBN) involving the right half of the face, the jugal mucosa and the lower eyelid with a tumour that had infiltrated the bone and the...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2005.029488
更新日期：2007-01-01 00:00:00
abstract：AIM:The evaluation of allelic losses at the FHIT and the BRCA1 genes and at three other loci at the 17q region in a series of 34 sporadic breast cancer cases from Southern Brazil. METHODS:The samples were evaluated for loss of heterozygosity (LOH) at the FHIT and the BRCA1 genes and at three other microsatellite marke...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2003.013490
更新日期：2004-04-01 00:00:00
abstract：:Three strains of Past. septica were isolated in Hong Kong. The clinical histories and the detailed bacteriological and serological studies are presented. ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.17.2.107
更新日期：1964-03-01 00:00:00
abstract：AIMS:Accurate assessment of HER2 status is essential for selection of patients for HER2-targeted treatment such as trastuzumab. This study investigated the hypothesis that delayed fixation impairs HER2 assessment. METHODS:9 carcinomas were received fresh, and samples were fixed immediately or put in fixative at time i...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2013-201978
更新日期：2014-07-01 00:00:00
abstract：AIM:To assess the topographical relation between gastric glands, using the minimum spanning tree (MST), to derive both a model of neighbourhood and quantitative representation of the tissue's architecture, to assess the characteristic features of gastric atrophy, and to assess the grades of gastric atrophy. METHODS:Ha...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.51.12.895
更新日期：1998-12-01 00:00:00
abstract：:Renal endocrine tumours are extremely rare, and carcinoid tumoral elements in renal cell carcinoma have never been reported. This is the first report of a composite renal cell carcinoma containing a clear cell renal cell carcinoma associated with carcinoid tumoral elements, in a patient with synchronous metastatic dis...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2009.070292
更新日期：2010-02-01 00:00:00
abstract：AIMS:To investigate proliferative activity in leukaemic and lymphomatous bone marrow infiltrates and to assess the feasibility of transport of specimens among institutions. METHODS:Proliferative activity in bone marrow trephine cryosections from 99 patients with non-Hodgkin's lymphoma (NHL), 23 patients with acute mye...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.47.3.209
更新日期：1994-03-01 00:00:00
abstract：:Twenty-two known strains of the Bacteroides fragilis group of organisms and 67 clinical specimens from a variety of sites were examined by fluorescent antibody test (IFA) using two different antisera fro the rapid detection of B fragilis group of organisms. A previously reported Barts' pooled antisera was compared wit...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.35.3.304
更新日期：1982-03-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:There has been a steady decline in the overt teaching of many basic and pathology sciences in the medical curriculum worldwide. As interns are the doctors most likely to request and act on tests, an assessment of their confidence in dealing with laboratory investigations was undertaken. METHOD...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,多中心研究
doi：10.1136/jcp.2009.064824
更新日期：2009-07-01 00:00:00
abstract：:An immunohistochemical study of the tissue CEA content of 82 epithelial neoplasms of the ovary has shown that mucinous tumours contain more of this substance than do their serous counterparts; otherwise a knowledge of tissue CEA content appears to be of little value in the differential histological diagnosis of this g...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.32.9.918
更新日期：1979-09-01 00:00:00
abstract：:Sera obtained from 34 men with Chlamydia trachomatis positive non-gonococcal urethritis, 34 men with C trachomatis negative non-gonococcal urethritis, 42 women with acute salpingitis, 38 healthy women, and 34 healthy men were studied for the presence of specific serum C trachomatis IgA and IgG antibodies. Serological ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.37.6.686
更新日期：1984-06-01 00:00:00
abstract：AIMS:To determine the sensitivity of commercially available diagnostic assays for Chlamydia trachomatis using a simple method. METHODS:Nine commercial assays and an "in-house" polymerase chain reaction (PCR) were evaluated using serial dilutions of a laboratory grown H serovar--four of them using a laboratory grown E ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.46.5.408
更新日期：1993-05-01 00:00:00
abstract：:The urinary excretion of the renal proximal tubular enzyme, gamma-glutamyltransferase (gamma-GT), has been studied in 41 patients receiving tobramycin, gentamicin or streptomycin for a variety of infections. All patients receiving tobramycin or gentamicin have shown increased excretion of gamma-GT in the urine. Only 4...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.30.5.432
更新日期：1977-05-01 00:00:00
abstract：:The indirect immunofluorescence technique was used to study the expression of the blood group A and H antigens in fresh-frozen and formalin-fixed paraffin-embedded sections of the bladder mucosa of 21 patients with non-neoplastic diseases of the bladder. Reliable assessment of these antigens could only be made using c...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.36.8.873
更新日期：1983-08-01 00:00:00
abstract：:This report describes the case of a 76 year old man who suffered from febrile ulceronecrotic Mucha-Habermann disease (FUMHD). Despite this patient's typical clinical and histological findings, the fulminating course led to death. Polymerase chain reaction (PCR) analysis of the skin lesions showed that the infiltrating...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.56.10.795
更新日期：2003-10-01 00:00:00
abstract：:Seven commercially available screening tests for rheumatoid factor and two quantitative tests have been compared with the standard Rose-Waaler test in a series of 477 sera from patients with various joint diseases. Markedly different results were obtained, and on the basis of our findings suggestions are made as to wh...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.27.5.368
更新日期：1974-05-01 00:00:00
