Improved safety and titre of murine leukaemia virus (MLV)-based retroviral vectors.

Abstract:

:Many retroviral vectors based on murine leukaemia virus (MLV) contain the first 420 nucleotides of the gag gene, as this was reported to increase vector titre by increasing the efficiency of RNA packaging. In this study, deletion of this gag sequence from its original location did not decrease the titre of two retroviral vectors, pBabe puro and MFG-S-. The two vectors could be improved by replacing the gag sequence with a CTE from Mason-Pfizer monkey virus (MPMV). This substitution improved vector titre, while eliminating a region of homology between vector and packaging constructs. Gene Therapy (2000) 7, 300-305.

journal_name

Gene Ther

journal_title

Gene therapy

authors

Zhao Y,Low W,Collins MK

doi

10.1038/sj.gt.3301081

keywords:

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

300-5

issue

4

eissn

0969-7128

issn

1476-5462

journal_volume

7

pub_type

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