Abstract:
:Many retroviral vectors based on murine leukaemia virus (MLV) contain the first 420 nucleotides of the gag gene, as this was reported to increase vector titre by increasing the efficiency of RNA packaging. In this study, deletion of this gag sequence from its original location did not decrease the titre of two retroviral vectors, pBabe puro and MFG-S-. The two vectors could be improved by replacing the gag sequence with a CTE from Mason-Pfizer monkey virus (MPMV). This substitution improved vector titre, while eliminating a region of homology between vector and packaging constructs. Gene Therapy (2000) 7, 300-305.
journal_name
Gene Therjournal_title
Gene therapyauthors
Zhao Y,Low W,Collins MKdoi
10.1038/sj.gt.3301081keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
300-5issue
4eissn
0969-7128issn
1476-5462journal_volume
7pub_type
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